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Citations for Biotium Inc. :
151 - 200 of 227 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cells were centrifuged again and stained with staining solution - propidium iodide (20μg/ml, Biotium), RNase A (1mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with primary antibodies diluted in TrueBlack® IF Blocking Buffer (Biotium) for 2 hours at RT ...
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bioRxiv - Immunology 2024Quote: All bacteria-cell lysates were treated with propidium monoazide (PMA, Biotium, Fremont, CA, USA), a photoreactive dye that effectively eliminates PCR amplification of DNA from dead cells ...
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bioRxiv - Immunology 2024Quote: ... cells were first stained with Live-or-Dye™ 405/545 (Biotium, Fremont, CA) followed by staining with PE anti-human CD69 (clone ...
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bioRxiv - Cell Biology 2020Quote: ... Cell populations deposited onto each slide were stained with GelGreen (P/N 41005; Biotium, Inc.) following manufacturer’s protocols ...
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bioRxiv - Bioengineering 2022Quote: Viability of encapsulated cells was measured by staining with Live/Dead viability assays (Biotium, USA), a cellular membrane integrity assay that stains live cells with green fluorescence and dead cells with red fluorescence ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were analyzed using the Steady-Luc™ Firefly HTS Assay Kit (Biotium, Freemont, CA). Luminescence was measured using a Synergy2 plate reader (Biotek Inc ...
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bioRxiv - Immunology 2022Quote: ... Tumor cells were labeled with CellBrite® Green membrane dye (Biotium Inc, Fremont, CA, USA) (1:2000 ...
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bioRxiv - Pathology 2021Quote: ... Cell surface area was measured by rhodamine conjugated wheat germ agglutinin (WGA) (1:1500, Biotium) staining using ImageJ5 ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were then incubated with 10 kDa CF640R-labeled dextran (100 ug/mL, Biotium #80115) for 15 minutes at 37°C in the presence of DMSO or SAR405 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with secondary antibodies diluted in TrueBlack® IF Blocking Buffer (Biotium) for 1 hour at RT ...
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bioRxiv - Physiology 2024Quote: ... cells were loaded with 1 μM Ca2+-specific dye Rhod2-AM (50024, Biotium, Fremont, CA) that preferentially localizes in mitochondria ...
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bioRxiv - Microbiology 2023Quote: ... The cells were lysed using lysis buffer supplied with Renilla Luciferase Assay Kit 2.0 (Biotium). 20 µl of cell lysate was mixed with 100 µl of freshly prepared working solution and the luminescence was recorded using a TECAN spectrophotometer.
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bioRxiv - Immunology 2023Quote: ... cells were stained in DMEM 1% penicillin/streptomycin with 5 nM MitoView 533 (Biotium, #70055). Plates were incubated at 37°C for 30 min protected from light ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were replaced with medium containing 5 µM NucView 488 caspase 3 substrate (Biotium Inc., #10403). After 30 min incubation at room temperature ...
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bioRxiv - Cell Biology 2020Quote: Mitochondrial membrane potential was assessed in live cells using the fluorescent dye tetramethylrhodamine (TMRM; Biotium 70017). Briefly ...
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bioRxiv - Physiology 2021Quote: ... [Ca2+]m was measured in cells loaded with Ca2+ specific dye Rhod2-AM (Biotium, Cat #50024) (1 μM ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were stained with Lysotracker red (Thermo) or Alexa594-labeled cholera toxin subunit B (CTB, Biotium) according to manufacturer’s protocols ...
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Proteasomal degradation of human SERINC4: a potent host anti-HIV-1 factor that is antagonized by NefbioRxiv - Microbiology 2020Quote: ... cells were lysed and intracellular luciferase activities were determined using Firefly Luciferase Assay Kit 2.0 (Biotium). These luciferase activities were used to calculate viral infectivity.
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bioRxiv - Microbiology 2023Quote: ... the cells were treated with 1 µg ml-1 nuclear stain NucSpot 650/665 (#41034, Biotium) for 30 min at RT ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were then mounted onto glass slides with EverBrite mounting medium with DAPI (Biotium Cat #23002), and ready for imaging ...
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bioRxiv - Bioengineering 2023Quote: Organoids were assayed for cell viability using a live/dead reagent kit (Biotium, Catalog #30002-T). Please see Sup ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed cells were additionally stained with membrane marker CellBrite (dilution 1:200, Biotium, Fremont, CA, USA) and actin marker Phalloidin (dilution 1:1000 ...
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bioRxiv - Biophysics 2024Quote: ... the cells were incubated in AnV buffer (1:175 dilution of the fluorescently tagged AnV (Biotium) in Hank’s balanced salt solution ...
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bioRxiv - Genomics 2023Quote: ... Cells were then incubated in 0.1 mg/ml Wheat Germ Agglutinin (WGA) CF770 conjugate (Biotium 29059) and 0.5 μg/ml DAPI (Abcam ab285390 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed twice and loaded with 25 nM TMRM and either 5 μM Nucview 488 (Biotium) or 5 μM Fluo4-AM (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed several times for 2hrs in BB and mounted on glass with EverBrite (Biotium). For primary and secondary antibodies see Immunofluorescence microscopy and antibodies section.
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bioRxiv - Bioengineering 2022Quote: ... the plasma membrane of live cells were stained with Wheat Germ Agglutinin (WGA) (CF633, Biotium, Fremont, CA). Cells were washed twice in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Microbiology 2021Quote: ... cells were stained with Wheat Germ Agglutinin (WGA) Alexa Fluor 488 Conjugate (Biotium; Catalog no. 29022-1) at a final concentration of 0.1 mg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... the plasma membrane of live cells were stained with Wheat Germ Agglutinin (WGA) (CF633, Biotium, Fremont, CA). Cells were washed twice in Hank’s balanced salt solution (HBSS ...
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bioRxiv - Cell Biology 2022Quote: ... cells were pre-treated 16-20 h with CellBrite®650 according to the manufacturers’ instructions (Biotium). For the analysis of ALIX-vesicle motility ...
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bioRxiv - Molecular Biology 2023Quote: ... Then, cells were resuspended in PI Staining Solution (3.8mM sodium citrate, 50 μg/ml PI (Biotium, USA), 40 μg/ml RNase A (Zymo Research ...
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bioRxiv - Physiology 2023Quote: ... Imaging of microtubules was performed by staining the microtubules with ViaFluor Live Cell Microtubule Stain 647 (Biotium) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were blocked for 1 hour at room temperature with 1x Fish Gelatin Blocking Agent (Biotium, #22010) in wash buffer ...
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bioRxiv - Molecular Biology 2023Quote: TUNEL assay was used to detect cell apoptosis using the CF605R TUNEL Assay Apoptosis Detection Kit (Biotium) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed twice with 1X PBS and then incubated with TrueBlack IF Background Suppressor (Biotium) for 30 minutes at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then incubated with 25 µM Alexa Fluor 568-hydroxylamine (Fluoroprobes) or CF680R-Aminooxy (Biotium) in the presence of 10 mM aniline (Sigma ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with 1 µg/mL of wheat germ agglutinin (WGA) conjugated to CF568 (Biotium; #29077-1) and 1 µg/mL anti-CD154 (CD40L ...
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bioRxiv - Plant Biology 2021Quote: ... protonemal cells were stained by the 5 µM of fluorescent dye SynaptoGreen C4 (equivalent to FM1-43, Biotium, USA ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were washed twice with PBS and incubated with PBS containing 50 μg/mL propidium iodide (Biotium, 40017) and 100 μg/mL RNase A (Omega ...
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bioRxiv - Neuroscience 2022Quote: Lysosomal activity was measured for the BV2 cell line and iMGLs using the LysoViewTM 540 reagent (Biotium, 70061) and Incucyte S3 Live-Cell imaging ...
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bioRxiv - Biophysics 2023Quote: ... After removal of excess NeutrAvidin the flow cell is incubated with 150 pM biotinylated NTA beads (#90074, Biotium) for 3 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1 h at 37°C in mKRB supplemented with native coelenterazine (5 µM, BIOTIUM), and then incubated for 15 minutes in mKRB supplemented with either TPEN (500 µM ...
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Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: ... cells were rinsed and 1:1000 CellBrite Steady 650 membrane dye and 1:1000 kit enhancer (Biotium #30108) was added in Fluorobrite DMEM +10% FBS for 30 minutes at 37℃.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then washed with annexin-binding buffer and additionally incubated with NucSpot Live 650 (1:1,000; Biotium) for 1 hr at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with PBS three times and coverslips were mounted onto glass slides in EverBrite Mounting Medium (Biotium). Cells were imaged using an EVOS FL microscope (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... The Live-or-DyeTM Zombie Aqua fixable viability staining kit was used for live cell analysis (32004-T; Biotium). For quantification ...
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bioRxiv - Biophysics 2023Quote: ... the growth medium was removed and cells were incubated with 1 μM CellBrite™ NIR680 (Biotium, Fremont, CA, USA) for 20 min ...
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bioRxiv - Immunology 2023Quote: ... Dead cells and doublets were excluded from analyses based on staining of 7-amino-actinomycin D (7-AAD) (Biotium) or Zombie yellow (catalog ...
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bioRxiv - Bioengineering 2023Quote: ... The cell viability was documented via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA).