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Citations for Biotium Inc. :
51 - 100 of 227 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... postsynaptic nicotinic receptors (nAChRs) were labeled using α-Bungarotoxin (BTX) conjugated with CF405 (4 μg/mL; Biotium; cat ##00002) for 45 min in PBS ...
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bioRxiv - Systems Biology 2021Quote: Genomic DNA was quantified using Sybr Green fluorescence assay with a 6-point DNA standard curve (0, 0.5, 1, 2, 4, 6 ng/μL Biotium). 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1X SYBR Green (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... was loaded by immersing the tissue in a Tyrode’s solution containing the voltage-sensitive indicator/dye Di-4-ANEPPS (10 µM; Biotium) for at least 30 min at room temperature (20–22 °C) ...
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bioRxiv - Cell Biology 2020Quote: ... 4 μl of the digested DNA was mixed with 2 μl 1:100 in DMSO diluted GelRed™ (Biotium) dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
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bioRxiv - Molecular Biology 2021Quote: RNA was biotinylated using 40 μl (~40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium #90066-1) in the following reaction ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
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bioRxiv - Biochemistry 2023Quote: ... DNA binding experiments using plasmid-length substrates were performed under the same conditions except with 100 ng DNA per reaction and were run on 0.8% agarose gel at 4 °C and post-stained with GelRed (Biotium).
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bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
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bioRxiv - Bioengineering 2023Quote: The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
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bioRxiv - Developmental Biology 2023Quote: ... to obtain single-cell suspensions.68,86 Cells were stained with a far-red cell membrane-permeant nuclear dye (RedDot™1, Biotium) to specifically select live cells and DAPI to exclude dead cells ...
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bioRxiv - Cell Biology 2023Quote: Cell growth quantification was performed using the ViaFluor 488 SE cell proliferation dye in minCMV reporter HEK293T cells (Biotium 30086) or CellTrace Far-Red proliferation dye in pEF reporter TC28a2 cells (Thermo C34564 ...
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bioRxiv - Cell Biology 2022Quote: ... at a 1:2000 dilution in PBS with 4% BSA was added and incubated for 30 min together with CF405M-Phalloidin (Biotium) at a 1:100 dilution to label F-actin ...
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bioRxiv - Microbiology 2021Quote: ... at 80°C for 10 minutes in 10−4 final dilution of commercial stock as described earlier (35) or with GelRed nucleic acid gel stain (Biotium,10−4 final dilution of commercial stock ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated at 4°C for 4 h with CF488-conjugated anti-mouse IgG (1:2000) and CF568-conjugated anti-rabbit IgG (1:2000) antibodies (Biotium) diluted in PBS containing 2% FBS as secondary antibodies ...
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bioRxiv - Immunology 2021Quote: ... the inserts were transferred to a fresh 24-well plate with black walls containing 500 μl of 4 μM Calcein AM (Biotium) in mHBSS per well ...
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bioRxiv - Genomics 2019Quote: ... Cells were loaded with MembriteTM Fix 488/515 cell surface staining kit (Biotium) per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the Biotium XTT Cell Viability Kit (Biotium 30007).
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bioRxiv - Biochemistry 2020Quote: ... 16 xg at 4°C and the pellet resuspended and incubated in WGA488 stain (Biotium, 5 μg/mL, diluted in HBSS) for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times to remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... antibodies were used at 1μg/mL overnight at 4°C and were subsequently detected with STORM-appropriate secondary antibodies (Biotium; 20836 and 20811). STORM acquisition was facilitated with an N-STORM system (Nikon Instruments ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was assessed after 5 days by XTT Cell Proliferation Assay (Biotium, Fremont, CA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... cells were stained with ViaFluor® 488 live cell microtubule stains (#70062, Biotium, Fremont, CA) following manufacturer’s instructions ...
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FlhE functions as a chaperone to prevent formation of periplasmic flagella in Gram-negative bacteriabioRxiv - Microbiology 2024Quote: Cells were stained with the Viability / Cytotoxicity Assay Kit for Bacteria Live & Dead Cells (Biotium). Cells were stained according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2021Quote: Viability of cardiac PCs and CAECs exposed to 1 μg/mL (5.8 nM) S protein or PBS vehicle was evaluated using the Viability/Cytotoxicity Assay Kit (Biotium #30002), according to manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... one mg of eluted protein at a concentration of 50-150 µM was incubated with a 2× molar excess of CF660R (Biotium) or Cy3B (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ... multi-shank neural probe (NeuroNexus A4×4-3mm-100-125-177-A16, Ann Arbor, MI, USA) pre-coated with DiI (Biotium, Fremont, CA, USA) for fluorescent detection of recording sites was advanced dorsoventrally at 5 µm/s through the IC using a hydraulic micropositioner (Model 650 ...
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bioRxiv - Immunology 2024Quote: ... cell viability was quantified using the ATP-Glo™ Bioluminometric Cell Viability Assay (Biotium #30020-2) on a CLARIOstar plate reader.
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bioRxiv - Cell Biology 2020Quote: ... ethidium homodimer (necrotic cells) (Biotium, Inc), and Draq5 (nuclei ...
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bioRxiv - Neuroscience 2023Quote: ... all proteins were used at a final concentration of 5 µM for this assay and GloMelt™ Thermal Shift Protein Stability Kit (Biotium) was used at final concentration of 0.5X ...
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bioRxiv - Genomics 2023Quote: ... cells were washed with DPBS and lysed directly in Qiazol reagent using a sterile miniature cell scraper (Biotium). Cell lysate was collected and stored for subsequent RNA processing and analysis.
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bioRxiv - Synthetic Biology 2023Quote: ... Both treated cells and control cells were stained with wheat germ agglutinin (WGA) conjugates CF568 (Biotium 29077-1) to visualize the plasma membrane ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with MitoView red (Biotium) and the mitochondrial length and area were measured by using Image J software (detailed protocol in Supplemental Materials and Methods).
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bioRxiv - Cancer Biology 2022Quote: ... Cell nuclei were visualized with Hoechst (Biotium) and sections were mounted using Fluoromount G (Southern Biotech) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell nuclei were visualized with Hoechst (Biotium) and sections were mounted using Fluoromount G (Southern Biotech).
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with CF405M Phalloidin (Biotium) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were stained with LysoView 488 (Biotium) for 1 h at 37°C according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Unconjugated primary antibodies CD23 and IgM were conjugated with Mix-n-Stain fluorescent protein tandem dyes antibody labeling kit for APC-CF750T (Biotium, Fremont, CA, USA) and Mix-n-Stain cf dye antibody labeling kit for CF405M (Biotium ...
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bioRxiv - Cell Biology 2019Quote: ... freshly thawed cells were diluted at 1×106 cells/mL and CellBrite TM NIR790 Cytoplasmic Membrane Dye (Biotium, cat.30079) was added at the concentration of 1µM and incubated at 37°C for 20 minutes with frequent agitation ...
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bioRxiv - Biochemistry 2021Quote: ... The pHi of ArchT transfected RPE cells and control RPE cells were directly calculated using 2’,7’-bis(carboxyethyl)-5(6)-Carboxyfluorescein (BCECF, Biotium). BCECF is a ratiometric dual excitation/single-emission pH biosensor (405 ex/525 em for pH insensitive fluorescence ...
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bioRxiv - Bioengineering 2022Quote: ... according to the manufacturer’s protocol and purified as described previously 45.The ACE2 or DnbALFA proteins were labeled by CF®640R succinimidyl ester dye (Biotium, USA, cat. 92108) in 0.1 M bicarbonate buffer using a 1:4 protein to dye ratio ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed in 2.5% paraformaldehyde (PFA) (Biotium) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were stained with CF633 (Biotium, USA) fluorescently labeled antibodies at 8 µg/ml in 1 % bovine serum albumin in PBS supplemented with 0.1 % Tween-20 for 1–3 h at room temperature in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with CF568-conjugated phalloidin (Biotium) and DAPI (Biotium ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were separately labelled using CellBrite™ (Biotium, USA) Red and Orange dyes for two-color experiments and CellBrite™ (Biotium ...
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bioRxiv - Genetics 2021Quote: ... Cells were incubated with 150 nM TMRE (Biotium, 70005) for 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated in LipidSpot488 (300-1000×, 70065, Biotium) and SYBR Gold (300-1000× ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were supplemented with NucView (Biotium #10402, 1 μM) and the indicated drugs (crizotinib (1 μM) ...
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bioRxiv - Microbiology 2020Quote: ... cells were mounted with Everbrite Hardset Mounting Medium (Biotium) overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were permeabilized using Permeabilization Buffer (Biotium #22016) for 10 minutes at room temperature ...