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Citations for Biotium Inc. :
1 - 50 of 227 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cells were fixed with 4% paraformaldehyde (Biotium, 22023). For lysosomal staining ...
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bioRxiv - Cancer Biology 2023Quote: Cells were fixed in 4% paraformaldehyde (PFA) in PBS (Biotium 22023) for 15 minutes at room temperature followed by pre-cooled (-20°C ...
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bioRxiv - Molecular Biology 2024Quote: Cells were fixed with 4% paraformaldehyde and stained with Phalloidin-CF594 conjugate (Biotium), DAPI (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were fixed with 4% PFA and stained with CF532 Wheat Germ Agglutinin (WGA) Conjugates (Biotium) and DAPI and imaged under a confocal microscope (Leica SP8) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were pre-incubated with Fura-2·AM or Fluo-4·AM (30 minutes, 1 μM, Biotium) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were exposed to 10 μM SynaptoRed™ C2 (Equivalent to FM®4-64; Biotium; 70027) in Stimulation Buffer consisting of 31.5 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... Cells that were imaged were labeled using CF640-conjugated streptavidin to label biotinylated proteins (CF640R Streptavidin, Biotium cat 29041). Affinity purification was performed using streptavidin-conjugated magnetic beads (Pierce ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
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bioRxiv - Biophysics 2022Quote: ... The protein band was stained by One-Step Blue Protein Gel Stain (Biotium, Fremont, CA).
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bioRxiv - Microbiology 2021Quote: ... fixed with paraformaldehyde (PFA) at 4% (Biotium) and glutaraldehyde 1% (Sigma Aldrich/Merck ...
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bioRxiv - Physiology 2023Quote: ... di-4-ANEPPS (7.5 µM, Biotium, CA) and positioned to center the anterior descending artery within the field of view ...
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bioRxiv - Bioengineering 2022Quote: ... 4-chlorobenzenesulfonate salt) dye was purchased from Biotium and dissolved in DMSO at 10 mg/mL prior to use ...
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bioRxiv - Neuroscience 2023Quote: ... organoids were fixed in 4%PFA (Biotium, 22023) for 3h at RT and then blocked for 1.5h at RT in 0.3% Triton-X and 10% horse serum (catalog#H0146 ...
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bioRxiv - Genomics 2022Quote: ... Total protein level of each lane was normalized by VersaBlot™ Total Protein Normalization Kits (Biotium Cat. # 33025).
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bioRxiv - Cancer Biology 2021Quote: ... 4 µl 5x HOT FIREPol EvaGreen qPCR Supermix (Biotium), 0.5 µl forward (10 µM stock ...
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bioRxiv - Developmental Biology 2020Quote: ... Labeling of protein carbonyl groups was obtained by overnight incubation of 20μg protein extracts with CF555DI hydrazide (Biotium, Fremont, CA, USA, #92165) at 4°C under medium agitation ...
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bioRxiv - Bioengineering 2020Quote: ... The voltage-sensitive dye di-4-ANEPPS (Biotium, Catalog number #61010) was loaded into the heart via the coronary circulation ...
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bioRxiv - Microbiology 2021Quote: ... containing 0.5 μg/ml DAPI (4’,6-diamidino-2-phenylindole, Biotium). Images were acquired on an LSM 700 confocal microscope (Zeiss ...
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bioRxiv - Microbiology 2021Quote: ... These samples were fixed with 1.2 ml of paraformaldehyde 4% (Biotium) for 30 minutes ...
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bioRxiv - Physiology 2019Quote: ... the heart was loaded with di-4-ANEPPS (Biotium, Inc., Hayward, California). A charge-coupled device (CardioCCD ...
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bioRxiv - Cell Biology 2021Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (10µg/ml, Biotium, cat. number 40043) was used for nuclear staining ...
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bioRxiv - Cell Biology 2023Quote: ... After nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (40043, Biotium) in IF buffer at a final concentration of 0.5 µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... After nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (40043, Biotium) in IF buffer at a final concentration of µg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... and DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate, Biotium, 60014-10mg) were dissolved in 100% ethanol (Koptec ...
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bioRxiv - Immunology 2021Quote: ... stained with one-step blue protein gel stain (Biotium, ref. 21003), and by SEC-HPLC with an xbridge protein BEH SEC (Waters ...
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bioRxiv - Genetics 2021Quote: ... 40 μl of 1X 4’,6-diamidino-2-phenylindole (DAPI; Biotium; Reagent Table) was added and the slides were sealed with a coverslip and clear nail polish.
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bioRxiv - Biophysics 2022Quote: ... growth medium was replaced with 4%PFA in PBS (Biotium, 200ul per well) for 20 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... 2016) using 40 μl (∼40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium) in the following reaction ...
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bioRxiv - Cell Biology 2021Quote: Staining of vacuoles: SynaptoRed™ C2 (Equivalent to FM®4-64) (Cat. # 70021, Biotium) was added to the growth media of cells (1 × 10^6 cells/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... 2015) using 40 μl (∼40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium) in the following reaction ...
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bioRxiv - Cell Biology 2021Quote: ... and 4 uL of 1 mM of SGC5 (Cat. number 70057, Biotium Inc., Fremont, CA) was injected into the AC of an enucleated mouse eye ...
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bioRxiv - Genomics 2023Quote: ... 64 using 40 μl (∼40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium) in the following reaction ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated for 20 hours at 4°C with anti-IgG (CF640R, Biotium Cat# 20177 ...
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bioRxiv - Neuroscience 2022Quote: ... Human neuronal cultures were fixed on coverslips in 4%PFA (catalog#22023, Biotium, Fremont, CA, USA) for 1h at RT and then blocked for 1.5h at RT in 0.1% Triton-X and 10% horse serum (catalog#H0146 ...
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bioRxiv - Biophysics 2023Quote: ... we fixed the samples using a fixative buffer composed of 4% Paraformaldehyde (PFA, Biotium, Freemont, CA) supplemented with 2.5% of Glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μg of RNA was biotinylated with 4 μg of MTSEA Biotin XX (Biotium Cat# 90066). The biotinylated RNA was isolated by binding to 80 μl of a Dynabeads MyOne Streptavidin C1 bead suspension (Invitrogen Cat# 65001 ...
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bioRxiv - Cell Biology 2021Quote: ... Protein gels were transferred onto nitrocellulose membranes and subsequently stained with Ponceau S (Biotium) before blocking in 5% BSA (Sigma ...
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bioRxiv - Biochemistry 2022Quote: Thermal melt measurements were using a GloMelt Thermal Shift protein stability kit from Biotium on a Qiagen Rotor-gene Q qPCR instrument ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... protein expression of the FRHΔ100 plasmid was induced with 0.2 mM IPTG (Biotium, 10021), at 18°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... the gels were re-stained with One-Step Lumitein™ Protein Gel Stain (Biotium) as per manufacturer’s directions and imaged again as before.
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bioRxiv - Genetics 2023Quote: ... Total protein stain was then conducted by diluting CF568-labeled succinimidyl ester (Biotium, 92131) at 1:100,000 in water containing 0.1 M NaHCO3 and 25 mM Na2CO3 ...
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bioRxiv - Neuroscience 2019Quote: ... then diluted to 6x) and run on agarose gels (1-2%) with 4% GelRed (Biotium, California, USA).
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... and incubated overnight at 4°C primary with antibodies diluted in TrueBlack® Antibody Dilutent (Biotium, 23013) with 0.2% Tween-20 ...
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bioRxiv - Biophysics 2023Quote: Conduction velocity (CV) was quantified by optical voltage mapping using di-4 ANEPPS (15 μM, Biotium #61010) as previously described.28 Briefly ...
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bioRxiv - Microbiology 2023Quote: For LipidSpot fluorescence imaging, live cells (XH001 cells, XH001/TM7x cells) were stained with LipidSpot (Biotium) at a 1×working concentration for 3-5 hours ...
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bioRxiv - Evolutionary Biology 2023Quote: ... pH 7.9) and subjected to SDSCPAGE followed by Lumitein Protein Gel Stain (Biotium, Hayward, CA, USA) and immunoblotting ...
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bioRxiv - Bioengineering 2023Quote: The melting temperature of purified nanoCLAMPs was determined using GloMelt Thermal Shift Protein Stability Kit (Biotium) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
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bioRxiv - Biophysics 2019Quote: FM 4-64 (SynaptoRed™C2) and FM 2-10 (SynaptoGreen™C2) were purchased from Biotium (Fremont, CA) to use as membrane probes ...