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Citations for VWR :
351 - 400 of 558 citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: Quadriceps from P10 pups were dissected and total proteins extracted using radioimmunoprecipitation assay (RIPA) buffer (VWR, Radnor, PA) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific) ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... The sheared DNA was then pre-cleared using magnetic beads (Sera-Mag Protein A/G SpeedBeads, VWR 17152104010150) for 1 hour to remove non-specific binding ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, catalog # 97062-376, VWR) (5 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... then tumors were measured every 2-3 days using a digital caliper (VWR, Radnor, PA, USA). Tumor volume was calculated with the formula 0.5 × L × W2 where L is the longest diameter and W is the diameter perpendicular to L ...
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bioRxiv - Neuroscience 2019Quote: ... The tissue was homogenized in 2 ml lysis buffer using a Dounce tissue grinder (7ml, VWR). This suspension was supplied with a 1.8M sucrose solution (4 mL) ...
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bioRxiv - Cancer Biology 2020Quote: ... Beads were washed 4X in Kischkel buffer + 2 μg/ml Aprotinin (VWR, Radnor, Pennsylvania, 97062-752) + 1 μg/ml Pepstatin (VWR 97063-246 ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Caco-2 cells were cultured in EMEM (ATCC, 30-2003) with 20% FBS (VWR 89510-186), 1% Pen/Strep (Life Technologies 15140122) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Supernatant was supplemented with 2 mM calcium chloride and filtered by a glass microfiber filter (VWR). Samples were then loaded by gravity flow onto 5 ml anti-protein C antibody affinity resin ...
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bioRxiv - Cell Biology 2019Quote: ... while covered with 1.5 ml of imaging media and 2 ml of white mineral oil (VWR). An objective heater (Bioptech ...
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bioRxiv - Microbiology 2020Quote: ... in antibiotic-free LB and 2 mL-deep-96-well plates in checkered pattern (VWR International). Plates were incubated at 37°C with 700 rpm shaking (Microplate Shaker TiMix 5 ...
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bioRxiv - Immunology 2020Quote: ... were separately stored in 2 mL pre-filled 2.4mm metal beads tubes (VWR International, Radnor, PA), and quickly snap-frozen in liquid nitrogen (40) ...
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bioRxiv - Cell Biology 2022Quote: ... glucose was replaced with 2% (w/v) galactose (ITW reagents) or 1.5% (w/v) raffinose (VWR), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... mice expressing hM4D(Gi) were intraperitoneally injected with 2 mg/kg CNO (89160; VWR International, LLC) and returned to their home cages ...
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bioRxiv - Cell Biology 2023Quote: ... cells grown in 2- or 4-well chamber slides (model 1 German borosilicate; Lab-Tek; VWR) were fixed with freshly prepared 4% formaldehyde in PBS solution (using 16% Methanol-free Formaldehyde ...
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bioRxiv - Neuroscience 2024Quote: ... pre-chilled 2 ml Pre-Filled Bead Tubes with 2.8 mm Ceramic Beads (VWR, Radnor, PA), and weighed ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were prepared according to the manufacturer’s instructions and proteins were separated in 10 % Next Gel SDS-PAGE (VWR). Carbonylated proteins were detected with Immobilon Western Chemiluminescent HRP Substrate (MilliporeSigma).
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bioRxiv - Immunology 2022Quote: ... The protein fraction was isolated by combining four times the volume of ice cold acetone (VWR Chemicals, PA, USA) to one volume of reconstituted PBMCsec ...
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bioRxiv - Systems Biology 2020Quote: ... Protein and crosslink digestion was carried out by adding 50 μL freshly prepared 20 mg/mL Proteinase K (VWR, freeze-dried powder suspended in 10 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins production was induced with 1 mM [F1] IPTG 3h [F2] treatment (Isopropil-β-D-tiogalattopiranoside, VWR, Pennsylvania, USA). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... cultured in 150mm dish (Alkali scientific TDN0150) for protein experiment and in 6-well culture plate (VWR, 100062-892) for total RNA experiment and transfected at around 50-60% confluency ...
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bioRxiv - Bioengineering 2023Quote: ... stably expressing enhanced green fluorescent protein (eGFP) were generated as described before[45] and cultured on 0.1% gelatin (VWR) pre-coated surfaces in MCDB-131 medium (Gibco ...
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bioRxiv - Genetics 2021Quote: ... Amplified emulsion mixture was broken and purified by adding 1 mL of 2-butanol (VWR, AA43315-AK), 50 μL of AMPure XP SPRI (Beckman Coulter ...
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bioRxiv - Genetics 2019Quote: ... All digested products were electrophoresed on 2% agarose gels using Agarose SFR™ (VWR Life Science, USA) for 2 hours at 80V ...
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bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...
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bioRxiv - Cell Biology 2022Quote: ... Transgene expression was induced with 24-hour incubation in media supplemented with 2 μg/ml doxycycline (VWR). SiRNA-mediated depletion of endogenous ESCO2 was done with 20 nM siRNA (Dharmacon ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplified cPCR products were examined on 2% agarose gels stained with GelRed (VWR International PBI, Milan, Italy) and visualized on a GelLogic 100 gel documentation system (Kodak ...
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bioRxiv - Cancer Biology 2022Quote: ... for 2 hours at 56°C and 50 μg of RNase A (VWR International, Radnor, PA, USA) for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... spectinomycin (100 µg/ml) and 0.2% arabinose in a 2 ml 96-well deep well block (VWR), sealed with breathable film (VWR ...
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bioRxiv - Developmental Biology 2021Quote: ... stained with 4’,6-diamidino-2-phenylindole (DAPI) in PBT (1:1000; VWR, 89139-054, Radnor, USA) for 10 minutes and washed with PBT twice for 15 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... an aqueous solution of sodium dodecyl sulfate (SDS; concentrations: 2% or 4%; Cat. No. 97064-472, VWR) was instilled directly into the trachea via an inlet cannula ...
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bioRxiv - Neuroscience 2020Quote: ... Cells used for in vitro electrophysiology experiments were grown on glass coverslips (12×12mm, No.2, VWR). Patch-clamp experiments were performed in aCSF consisting of (in mM) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 x 107 cells per replicate were resuspended in lysis buffer (8 M urea (VWR, 0568-500G), 50 mM Tris-HCl pH8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... then cells were resuspended in sterile PBS with 2% FBS and filtered (70 μm; VWR, #10204-924).
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bioRxiv - Biophysics 2023Quote: ... were soaked for 1 hour at ~ 60°C in 2% v/v cleaning detergent (MICRO-90, VWR), thoroughly rinsed with 18.2 MΩ ultra-pure water ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µL cell suspension was loaded under a 22 x 22 mm glass coverslip (VWR, thickness: 1.5).
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bioRxiv - Cancer Biology 2023Quote: ... and 0.5% Triton X-100) on ice for 2 min and immediately fixed in formaldehyde 4% (VWR) for 10 min at the room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... slabs were optically cleared using a 1:2 mixture of benzyl alcohol (Cat. No. 109626; VWR, Sweden) and benzyl benzoate (BABB ...
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bioRxiv - Cancer Biology 2024Quote: ... or tissue culture-treated T-25 flasks (2×106 cells/flask; Avantor/VWR, Cat. No. 10861-568) under the growth conditions described above ...
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bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
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bioRxiv - Systems Biology 2020Quote: ... 4°C) and the soluble fraction was collected and protein precipitated using ice-cold acetone (25% final concentration, VWR, USA) for 1h at - 20°C followed by centrifugation (400x g ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The proteins were detected by adding chemiluminescence detection reagent (ECL Amersham) to the membranes and exposing onto ECL Hyperfilm (VWR) for 30m.
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bioRxiv - Biophysics 2020Quote: ... the untreated and treated bacteria (expressing H-NS-mEos3.2 fusion proteins) were filtered by 0.2 μm filters (VWR International LLC), resulting in filtrates constituting leaked cellular contents due to membrane damage ...
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bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... pHrodo™ iFL Red STP Ester was diluted to 2 mM in DMSO (VWR Life Science, 97063-136). Antibody was brought up in DPBS (2 mg/mL final concentration for Dll4 polyclonal antibody [Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μL of cell suspension were loaded under a 22 x 22 mm glass coverslip (VWR, thickness: 1.5). Spinning disk confocal images of S ...
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bioRxiv - Biochemistry 2021Quote: ... AsLPMO10B and VSAL_I0902/AsChi18Bp by PCR amplification using Red Taq DNA polymerase 2× Master mix (VWR, Oslo, Norway) according to the manufacturers protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Trifluoroacetic acid, acetonitrile and 2-propanol were from Biosolve BV (Valkenswaard, The Netherlands) and glycine was from VWR International (Radnor ...
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... Equal amounts of plasmid (2 μg total) were co-transfected with 3 μL 25kDa PEI (VWR, Radnor, PA) into 293T cells into a 24-well plate.