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Citations for VWR :
201 - 250 of 558 citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... 2 Glass Coverslips (# 48382-085) were purchased from VWR. RNeasy mini prep kit (# 74106 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and human keratinocyte growth supplement (HKGS) 100X (S-001–5) from Cascade Biologist were procured from VWR Corporation (Missouri ...
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bioRxiv - Microbiology 2020Quote: ... We washed the pelleted biomass 1 – 2 times and resuspended it with 50 µL 1x PBS from which we fixed 2 µL on solidified agarose (VWR, Solon, OH, USA) (1% w/v) ...
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bioRxiv - Plant Biology 2020Quote: ... and all protein estimations were done using Bradford reagent (VWR Inc.).
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to a PVDF membrane (VWR, Cytiva, #10061-492), blocked with 5% powdered milk in TBST (50 mM Tris-HCl pH7.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.1% Rho-associated protein kinase (ROCK) inhibitor (Y-27632; VWR). Each EHT construct consisted of 1.0 x106 cells ...
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bioRxiv - Immunology 2020Quote: Cell protein was isolated using RIPA lysis buffer (VWR, Ontario, Canada) supplemented with protease inhibitor cocktail (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.1 mg/mL bovine serum albumin protein (VWR, 97062-508), then 1 hour with Pierce™ Protein A/G agarose beads (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred to nitrocellulose blotting membranes (10-6000-01, VWR) in Harlow buffer at 350mV and 4°C for 2 hours ...
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bioRxiv - Bioengineering 2019Quote: ... then wrapped them with 2” parafilm (VWR, cat no. 52858) and stored at room temperature for 1 day ...
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bioRxiv - Neuroscience 2019Quote: ... then transferred to 2% dimethyl sulfoxide (DMSO: VWR, Radnor, PA) solution for cryoprotection for 1-2 days ...
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bioRxiv - Evolutionary Biology 2019Quote: ... all media contained 2% dextrose (BD, VWR catalog #90000-904), 0.67% YNB with nitrogen (Sunrise Science ...
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bioRxiv - Biochemistry 2020Quote: ... HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (VWR, Radnor, PA), NaCl (VWR) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were plated on No.2 VistaVision cover glasses (VWR). For hydraulic chamber experiments and immunofluorescence ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... After centrifugation (500g, 2 min) (Centrifuge 5417C, Eppendorf, VWR, Belgium) to remove large particles ...
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bioRxiv - Cell Biology 2021Quote: ... plus 2% glutamine (BioWhittaker) and 10% bovine calf serum (VWR). Cos-7 cells (a gift of Dr ...
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bioRxiv - Microbiology 2022Quote: ... 1-BuOH ANALAR and 2-propanol HPLC grade from VWR international GmbH ...
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bioRxiv - Systems Biology 2023Quote: ... containing 2% formic acid (FA; ≥99%. VWR International, Vienna, Austria). The eluted samples were dried using a gentle nitrogen stream at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... a coverslip (VWR, No. 2, 181×8 mm, #48366-045) was coated with Poly-D-lysine (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... at 0.5 mg/mL in PBS + 2% sucrose (VWR, #27480.294). Coverslips were placed on a piece of parafilm ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were selected with 2 μg/ml puromycin (VWR, CAYM13884) for five days and subsequently split into the different screening condition arms ...
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bioRxiv - Immunology 2023Quote: ... The samples were stored in 2 ml ethanol (VWR, 20821.330P). Clean and fire-sterilized scissors and forceps were used for each intestinal section to minimise bacterial DNA contamination ...
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bioRxiv - Bioengineering 2023Quote: ... and the photoabsorber 2-isopropylthioxanthone (ITX, no. TCI0678; VWR International) (0.8 w/w) ...
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bioRxiv - Bioengineering 2024Quote: ... filtered with a 2 μm cellulose filter (VWR, #514-0061) and supplemented with 2 % fluorinated surfactant FluoSurf neat (Emulseo ...
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bioRxiv - Cell Biology 2021Quote: ... Separated proteins were transferred onto 0.45 μm nitrocellulose transfer membranes (VWR International). Protein loading and transfer quality were checked by staining with Ponceau S ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was isolated using Ni-NTA Resin (VWR Cat. No. 786-940) and eluted with 2 mL of elution buffer (300 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were dialyzed in a Slide-a-lyzer (VWR, 514-0172) overnight in ATPase buffer (100mM potassium acetate ...
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bioRxiv - Microbiology 2019Quote: ... Insoluble protein pellets were resolubilized in 3 M urea (VWR, Radnor, PA) and purified using immobilized metal affinity chromatography using Ni-NTA resin (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein samples were purified using HiPPR detergent removal resin (PI88305, VWR International) and dried completely by Speedvac (Savant ...
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bioRxiv - Cell Biology 2022Quote: ... Denatured proteins were then transferred to a Nitrocellulose membrane (Amersham Protran, VWR) by applying 300 mA for at least 60 min in a wet transfer chamber with blotting buffer (25 mM Tris pH 8.3 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), mouse anti-NeuN (Sigma # MAB377 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), rabbit anti-dsRed (Takara #632496 ...
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bioRxiv - Biochemistry 2019Quote: ... spectra of native, human BACE1 (6.12 μM, 30 μl; Acro Biosystems, USA) in 50 mM sodium acetate (pH 4.0; VWR, UK) was obtained using a J-1500 Jasco CD spectrometer and Spectral Manager II software ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Human foreskin fibroblasts (HFF, kindly provided by Denise Galloway) were maintained in Minimum Essential Media-α (MEM-α; VWR) supplemented with 20% FBS (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... with the only difference that 2 ml of mineral oil (VWR) were placed on top of the sealed prep to facilitate temperature detection with a thermocouple ...
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bioRxiv - Bioengineering 2021Quote: ... 20 μL of 2:1 methanol (ACS Grade, VWR BDH1135-4LG) to water (18.2 MΩ ...
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bioRxiv - Biochemistry 2021Quote: ... the samples were diluted 1:2 with MS grade water (VWR). Proteins were digested overnight at 37 °C by addition of 3 μg trypsin (Promega ...
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bioRxiv - Microbiology 2019Quote: ... To an autoclaved 2 mL microcentrifuge tube (VWR, Radnor, PA, USA), 500 µL of the supernatant was added ...
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bioRxiv - Bioengineering 2019Quote: ... which had to be blocked in 2% (w/v) BSA (VWR) + 0,05% tween-20 in PBS to work (data not shown) ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in PBS + 2% SDS + protease inhibitor cocktail (VWR, #97063-972), and transferred to 2 mL disruption tubes (OPS Diagnostics ...
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bioRxiv - Biophysics 2021Quote: ... 2 million cells were seeded into a 10 cm dish (VWR) and transfected with 8 μg hASIC1a WT ...
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bioRxiv - Cancer Biology 2021Quote: ... The fixed sections were covered with propan-2-ol (VWR, 20842312). Following evaporation for 40 seconds ...
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bioRxiv - Biochemistry 2021Quote: ... the samples were diluted 1:2 with MS grade water (VWR). Proteins were digested overnight at 37 °C by addition of 1 µg of trypsin (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... The fungal specimen was sandwiched between 2 cover glasses (VWR international).
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bioRxiv - Neuroscience 2022Quote: The brain was transferred to 2% dimethyl sulfoxide (DMSO; VWR, USA) solution for cryoprotection for 1-2 days ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were run on a 2% agarose (VWR, Cat# MPN605-500G) and 1X Tris-Acetate-EDTA (TAE ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in 50 µl 2 M urea (VWR, 0568-500G), 50 mM ABC.
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bioRxiv - Immunology 2023Quote: ... and resuspended in 50 µl 2 M urea (VWR, 0568-500G), 50 mM ABC ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were washed 2 times with Histochoice Clearing Agent (VWR, H103) and 4 times with isopropanol (VWR,9084-03 ...
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bioRxiv - Immunology 2023Quote: ... and 2 steps at 50% with reagent alcohol (VWR BDH1156-4LP)) into water ...