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Citations for VWR :
251 - 300 of 558 citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Caco-2 cells were seeded into 24-well Transwell plates (VWR) at a density of 1.5×105 cells/cm2 and grown at 37°C in 5% CO2 for 14 or 21 days for confluency ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred onto a 0.2μM nitrocellulose membrane (VWR: 27376-991). Membranes were blocked in 5% nonfat dairy milk in TBS-T ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... A vial of human primary nasal epithelial cells was thawed and aliquoted into multiple T25 cell culture flasks (VWR, UK). Cells were maintained in AECGM until confluent ...
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bioRxiv - Cell Biology 2021Quote: 3,000 ESCs/well mixed with 0.3 μg Wnt3a-beads/well were seeded onto human fibronectin (10 μg/mL, cat. um. 7340101, VWR/SLS) coated 8-well slides (cat ...
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bioRxiv - Neuroscience 2020Quote: ... Human neuronal cells SH-SY5Y (ATCC, Manassas, VA, #CRL-2266) were cultured in DMEM medium (VWR, Radnor, PA, #0101-0500) supplemented with 10% FBS (Gemini Biosciences West Sacramento ...
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bioRxiv - Microbiology 2019Quote: ... strains [69], were maintained in human foreskin fibroblasts (ATCC; Manassas, VA) in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA) supplemented with 10% fetal bovine serum (VWR) ...
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bioRxiv - Immunology 2020Quote: Cells of human or murine origin (0.2-1×106) were lysed in Trizol (peqGOLD TriFast™; VWR International, Radnor, USA) and total RNA was extracted by use of the DirectZol® RNA MiniPrep (Zymo Research ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were enriched by negative selection using StemCell RosetteSep Human Monocyte Enrichment Cocktail (StemCell 15068; 75μl per ml of blood) and Ficoll-Paque (VWR 17144003). Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034 ...
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bioRxiv - Microbiology 2023Quote: Toxoplasma strain TATi-RHΔku80 [41] was cultured in human foreskin fibroblasts in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA, USA) supplemented with 10% fetal bovine serum (VWR) ...
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bioRxiv - Genetics 2021Quote: ... Resulting PCR products were loaded on a 2% agarose (VWR, 97062-250) gel ...
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bioRxiv - Immunology 2021Quote: ... 2 mL of HEPES-buffered saline pH 7.0 (2X for transfection) (VWR) was added dropwise to the previous mixture ...
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bioRxiv - Microbiology 2019Quote: ... cracked corns (2 g) were put in 20 mL scintillation vials (VWR) and rehydrated with 1 mL sterilized water overnight followed by autoclaving ...
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bioRxiv - Microbiology 2022Quote: ... samples were stored in PBS containing 2 % formaldehyde (VWR Life Science AMRESCO). Acquisition of all samples was done on a BD FACSCelesta flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... samples were stored in PBS containing 2 % formaldehyde (VWR Life Science AMRESCO).
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bioRxiv - Microbiology 2022Quote: ... samples were stored in PBS containing 2 % formaldehyde (VWR Life Science AMRESCO). For the intracellular staining of infected U87.ACE2+ cells ...
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bioRxiv - Microbiology 2022Quote: All compounds (see supplemental table 2) were dissolved in DMSO (VWR V0231) and dispensed using an HP D300e digital dispenser in a 384-well plate format using a 2-fold dilution series ...
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bioRxiv - Bioengineering 2020Quote: ... Slices were then stained in Gill 2 hematoxylin solution (VWR: 10143-146) for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... 1-2 × 105 cells were plated in transwell inserts (VWR, Radnor, PA) coated with 0.3 mg/mL Corning™ Matrigel™ Membrane Matrix (Corning ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were permeabilized and blocked in 2% bovine serum albumin (BSA) (VWR) and 0.1% triton X (VWR ...
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bioRxiv - Genomics 2021Quote: ... to a 2 ml Phase Lock Gel Heavy tube (713-2536, VWR). The mixture was incubated for 5 min at 30°C at 1100 rpm ...
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bioRxiv - Immunology 2023Quote: ... overnight onto 96-well Immunlon-2 plates (VWR Scientific, Mississauga, ON, Canada). Recombinant protein antigens included SARS-CoV-2 FLS glycoprotein trimer [50 ng/well ...
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bioRxiv - Microbiology 2022Quote: ... samples were stored in PBS containing 2 % formaldehyde (VWR Life Science AMRESCO). Acquisition of all samples was done on a BD FACSCelesta flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... and crosslinked for 2 minutes using a UV Crosslinker (VWR, Radnor, PA). The blots were incubated with γ32-P 5’-labelled probe at 42°C overnight and imaged using a phosphor screen on a Typhoon FLA 7000 Variable Mode Imager System (GE Healthcare ...
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bioRxiv - Developmental Biology 2023Quote: ... We confirmed the amplification using 2% Agarose I™ (VWR, Philadelphia, PA) and gel electrophoresis ...
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bioRxiv - Developmental Biology 2023Quote: ... animals were soaked in 20 mg/mL 5-bromo-2’-deoxyuridine (VWR) with 3% DMSO in a 6 g/L Instant Ocean salt solution for 2 hours followed by a 4-hour chase prior to processing using the rapid protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... This mixture was transferred into a 2 mm sterile electroporation cuvette (VWR) chilled on ice ...
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bioRxiv - Cell Biology 2023Quote: ... samples were blocked with 2% bovine serum albumin (BSA, VWR, cat#97061), 1% DMSO ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was then transferred into a 2 mm electroporation cuvette (VWR) and pulsed at 21 kV/cm ...
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bioRxiv - Microbiology 2023Quote: All compounds (see supplemental table 2) were dissolved in DMSO (VWR V0231) and dispensed using an HP D300e digital dispenser in a 384-well plate format using a 2-fold dilution series ...
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bioRxiv - Microbiology 2024Quote: ... mechanically homogenized and afterward fixed with 2% Paraformaldehyde (10 min, RT; VWR). To minimize clumps ...
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bioRxiv - Biophysics 2023Quote: ... desalting with an ultracentrifuge filtration device (Vivacon 2 kD cut-off, VWR) was performed to remove residual Li+ cations and acetone.
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bioRxiv - Developmental Biology 2024Quote: ... with 2-4 coverslips (22 mm x 22 mm; VWR, 16004-094) on either side of the sample to avoid sample compression ...
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bioRxiv - Bioengineering 2024Quote: ... A cover glass (22 x 22 mm, #2; VWR, Radnor, PA, USA) offset with double-sided tape spacer ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins in the pellet were solubilized by sonication (incubation in Ultrasonic Cleaner bath, VWR) for 10 min followed by incubation for 60 min at 4 °C under rotation ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration throughout the study was determined via Bradford assay (catalogue number-VWR M172).
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using Amersham ECL Prime Western Blot Detection Reagent (VWR, CA89168-782) and a ChemiDoc Imaging System (Bio-Rad Laboratories).
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bioRxiv - Biochemistry 2023Quote: ... protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG, #437145X, VWR) at 16 °C overnight ...
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bioRxiv - Genomics 2020Quote: Single cell suspensions in PBS with 2% FBS were stained by incubating for 15 minutes at room temperature protected from light with anti-human PTPRC (CD45) monoclonal antibody conjugated to FITC (1:200 dilution, VWR #ABNOMAB12230), anti-human EPCAM antibody conjugated to PE (1:50 dilution ...
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bioRxiv - Microbiology 2023Quote: ... A surface capture lawn was prepared with 50 ug/mL of goat anti-Human IgG Fc secondary antibody (VWR, 103255-066) in 10 mM sodium acetate (pH 4.5)/0.01% Tween ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was removed and boiled with SDS (2% (w/v)) (VWR chemicals) to denature the protein ...
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bioRxiv - Immunology 2021Quote: ... and was stopped by addition of 2 M sulfuric acid (BCH7500-1, VWR) at 1:1 volume ratio ...
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bioRxiv - Molecular Biology 2021Quote: ... YPD medium uses YP medium components supplemented with dextrose (2% w/v, VWR). YPRaf medium uses YP medium components supplemented with raffinose (2% w/v ...
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bioRxiv - Bioengineering 2020Quote: ... chambers were put on a 2-dimensional rocking platform (VWR, Breda, The Netherlands) with a speed rate of 1 rotation per minute at an angle of 10° ...
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bioRxiv - Bioengineering 2020Quote: ... The devices were plasma bonded to 3 x 2 in glass slides (VWR) for drop makers and 25 x 25 mm type 0 coverslips (VWR ...
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bioRxiv - Microbiology 2021Quote: ... The grid was stained 3x with 5 μL of 2% uranyl acetate (VWR) with blotting immediately after application for the first two rounds of staining ...
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bioRxiv - Microbiology 2022Quote: ... This mixture was transferred to a 2 mm electroporation cuvette (89047-208, VWR), and electroporated at 2500 V ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently incubating the substrates in a 60% (v/v) 2-propanol (VWR) for 5 min ...
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bioRxiv - Genetics 2021Quote: ... Staining was performed with 10µM H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate, VWR #89138-260) for 1 hour ...
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bioRxiv - Biophysics 2020Quote: ... Before digestion the samples were diluted 1:2 with MS grade water (VWR). Samples were digested overnight at 37 °C with 1 µg trypsin (Promega).
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bioRxiv - Microbiology 2023Quote: ... and placed between the metal plates of a 2 mm electroporation cuvette (VWR). Three micrograms of p2NIL/ΔsecA2 GOAL was UV irradiated (exposed to 1000 μJ/cm2 in a CL-1000 Ultraviolet Crosslinker ...