Labshake search
Citations for VWR :
1901 - 1950 of 1957 citations for 8 BROMO 4 METHYLTHIO 7 PHENYLPYRAZOLO 1 5 A 1 3 5 TRIAZIN 2 AMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Single-species cultures were obtained by picking colonies using 1 µL sterile inoculation loops and transferred to 800 µL liquid isolation medium in 96 deep well 2 mL plates (VWR) which were incubated for at least 2 weeks on a bench top shaker (RT ...
-
bioRxiv - Biochemistry 2022Quote: ... 50 µL of each was added to 2 mL cultures of attached cells in 6-well plates (#10861-554, VWR) containing 0.2 mM CaCl2 ...
-
bioRxiv - Biochemistry 2022Quote: Each <3 kDa FBS extracted material was added to >30 kDa FBS fraction in 2 mL FBS-free media in the well of a 6-well plate (#10861-554, VWR) at roughly 10X the concentration they would exist in the standard growth media ...
-
bioRxiv - Synthetic Biology 2023Quote: Sender strains and receiver strains were inoculated in 1 mL of rich M9 medium supplemented with the appropriate antibiotics in a 2 mL deep-well 96-well plate (VWR), covered with a “Breathe Easier” membrane (Sigma) ...
-
bioRxiv - Plant Biology 2023Quote: ... Flash chromatography was carried out using a 2 mL glass pasteur pipette loaded with 200 mg of P60 silica gel (SiliCycle) with hexane (HPLC grade; VWR) as the initial mobile phase ...
-
bioRxiv - Cell Biology 2024Quote: ... This was followed by perfusion with Solution 2 consisting of DMEM supplemented with collagenase type I (0.8 mg/mL) (VWR International) for 7–8 mins at the same flow rate ...
-
bioRxiv - Neuroscience 2023Quote: ... for cryoprotection and allowed to sink at 4°C prior to being cryosectioned at 20 μm and placed on charged slides (VWR Superfrost® Plus, 48311-703).
-
bioRxiv - Neuroscience 2024Quote: To fix the tissue for post-hoc analysis, mice were transcardially perfused with phosphate buffered saline (PBS, Fisher BioReagents, CAT 10051163) followed by 4% formaldehyde (VWR Chemicals BDH®, CAT 9713.1000). The brains were dissected out and post-fixed in 4% formaldehyde overnight at 4°C ...
-
bioRxiv - Biochemistry 2019Quote: ... The column was held under isocratic flow for 10 mins prior to developing a linear gradient from 0 to 2 M NaCl (HPLC grade; VWR, UK) over 60 mins ...
-
bioRxiv - Bioengineering 2021Quote: ... cell suspensions were prepared at a concentration of 300,000 cells/mL in 1X phosphate buffered saline (PBS) solution supplemented with 2% fetal bovine serum (FBS, VWR 89510-186) to reduce cell-cell and cell-PDMS adhesion ...
-
bioRxiv - Microbiology 2020Quote: ... Pigment-expressing GBS was concentrated in 50 µL of PBS and a 2 µL droplet was aliquoted and sandwiched between two cover slides (48393-230, VWR International). Changes in pigment presence were determined by measuring the change in Raman peak amplitudes associated with conjugated double bonds located at the 1126 cm-1 and 1510 cm-1 before and after light treatment ...
-
bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int. LLC, Cat# 23486.297, and glycerine, VWR, Cat# 24387.292) in a refrigerator until sectioning ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were plated in 6-well plates at 1 million cells per well and the following day were transfected with 2 μg DNA using 6 μl of JetPrime (VWR International) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 and heated for 2 hrs at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001), (3 ...
-
bioRxiv - Cancer Biology 2022Quote: ... was added to an empty tube and heated for 2 hr at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001).
-
bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 (prepared from trace metal grade HNO3) and heated for 2 hrs at 90°C in a loosely capped 15 ml centrifuge tube (metal free, VWR, #490001), (2 ...
-
bioRxiv - Molecular Biology 2019Quote: ... cDNA was then mixed with gene-specific primers (2 μM) and Perfecta SYBR green SuperMix reagent (Cat # MP9505402K, VWR, Radnor, PA) for PCR amplification using a DNA Engine Opticon 2 system (MJ Research ...
-
bioRxiv - Developmental Biology 2020Quote: ... 10% formamide and 2x saline sodium citrate (SSC) supplemented fresh each experiment with 0.1% Tween-20 and 2 μg/mL nuclease-free BSA (VWR, 0332-25G)) ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... each chamber was filled with 100μl food medium containing a mix of 2% agarose (VWR life science; type number: 97062-250), 2% sucrose (Merck KGaA ...
-
bioRxiv - Microbiology 2019Quote: ... colonies were pooled by suspending the cells in 2 mL 0.9% NaCl per plate with a cell scraper (Corning®, VWR, Germany).
-
bioRxiv - Immunology 2020Quote: ... and the filter was washed with sterile 1x phosphate buffer saline (PBS) supplemented with 2% v/v fetal bovine serum (FBS, VWR, USA). Cell density was determined through trypan blue exclusion ...
-
bioRxiv - Biochemistry 2020Quote: ... The 168 h incubation samples were sonicated for 2 minutes in a water bath sonicator (VWR® Symphony™ Ultrasonic Cleaner). Circular dichroism (CD ...
-
bioRxiv - Immunology 2022Quote: ... 2-2.5 × 106 frozen or freshly isolated monocytes were seeded in 10 cm non-tissue culture treated dishes (VWR, #734-2796) in 10 mL RPMI 1640 (Biowest ...
-
bioRxiv - Synthetic Biology 2022Quote: ... boulardii cultures were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
-
bioRxiv - Synthetic Biology 2022Quote: Single and co-cultivation of (SbU-)-GFP and (SbU- + bts1Δ + thi6Δ)-mKate were cultured in 2 mL SC media YPD in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen ...
-
bioRxiv - Synthetic Biology 2022Quote: Single and co-cultivation of (SbU-)-GFP and (SbU- +bts1Δ + thi6Δ)-mKate were cultured in 2 mL SC media with and without thiamine in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 2 mL LB or BHI medium was inoculated with 20 µL preculture and grown in a 24-well plate (VWR, USA). Furthermore ...
-
bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
-
bioRxiv - Synthetic Biology 2022Quote: ... boulardii strains were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
-
bioRxiv - Microbiology 2023Quote: ... A total of 20 μg of proteins were mixed with 6x SDS loading dye containing 10% 2-mercaptoethanol (VWR Life Sciences) and boiled at 100 °C for 10 min before loading ...
-
bioRxiv - Physiology 2021Quote: ... and this portion was placed on top of a stack of 4-6 glass microscopy slides (depending on size of animal) (VWR, Radnor PA-Cat. No. 48300-026). Tissue was secured onto glass slide with a biorthogonal silicon elastomer (Kwik-Sil ...
-
bioRxiv - Bioengineering 2021Quote: ... The cells were plated to ∼2 x 105 cells per well in a 6-well VWR tissue culture-treated plates (VWR, Suwanee, GA) and incubated ∼12 hours (overnight) ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
-
bioRxiv - Developmental Biology 2020Quote: ... and then incubated with secondary antibodies and DAPI for 2 hr at room temperature prior to mounting in AquaPoly/Mount mounting medium (VWR, 87001-902).
-
bioRxiv - Neuroscience 2019Quote: ... the flies were first sated with water and then presented with 100mM sucrose containing 2% blue dye (McCormick) filled in a graduated capillary (VWR, #53432-604). Until the flies became unresponsive to 10 serial food stimuli ...
-
bioRxiv - Bioengineering 2021Quote: Plant or mammalian produced SARS-CoV-2 RBD protein were diluted in sterile 1X PBS to 2 μg/mL and coated onto a 96-well plates (VWR #62402-959) (50 μL /well ...
-
bioRxiv - Microbiology 2022Quote: ... Spleen and brain were homogenized in 1 ml of sterile PBS followed by culture of 10-fold dilutions of each tissue on 2X GYE agar (2% glucose (VWR™, USA), 1% yeast extract (BD™ ...
-
bioRxiv - Immunology 2023Quote: ... mice were whole body perfused through the heart with 2% paraformaldehyde (diluted down in PBS from 16%; VWR Cat. No. 15710-S), then livers were harvested and placed in 2% paraformaldehyde for 24 hours at 4°C then placed in 30% sucrose (Sigma-Aldrich Cat ...
-
bioRxiv - Physiology 2023Quote: ... after overnight fasting followed by administration of a saline solution of glucose (2 g/kg or 1.5g/kg, for rats or mice, respectively; VWR Chemicals, Leuven, Belgium), by gavage ...
-
bioRxiv - Neuroscience 2023Quote: ... membranes were washed 3x for 15 minutes with PBS-T (PBS with 0.02% Tween) and incubated for 2 hours with either anti-rabbit HRP-linked (VWR, cat. no NA934) or anti-mouse HRP-linked (VWR ...
-
bioRxiv - Bioengineering 2024Quote: ... was defined by completely demineralizing a 2% gelatin solution by adding an abundance of mixed bed ion exchange resin Amberlite IRN-150 (VWR, 40992.A9) to the gelatin ...
-
bioRxiv - Molecular Biology 2024Quote: ... 350 µl Iron Assay buffer and 20 µl 20% SDS were added to the liver pieces (50 mg) in 2 ml tubes for Precellys homogenisers (Ref. 432-0351, VWR/Bertin Instruments). Then ...
-
bioRxiv - Microbiology 2024Quote: ... Six clams taken from the acclimation aquarium were crushed together for 2 min at maximum speed using the Stomacher® 3500 instrument (VWR, Italy). Subsequently ...
-
bioRxiv - Plant Biology 2020Quote: Metabolite extraction was conducted by weighing out 25 mg of each freeze-dried peach mesocarp sample and placing them into clean 2 mL autosampler glass vials (VWR, Radnor, PA, USA). One mL of 80% by volume LC-MS grade methanol (MeOH ...
-
bioRxiv - Systems Biology 2021Quote: Metabolite extraction was conducted by weighing out 19 - 21 mg of each freeze-dried sorghum roots and placing them into clean 2 mL autosampler glass vials (VWR, Radnor, PA, USA). Automated control of sample extraction (i.e. ...
-
bioRxiv - Cell Biology 2020Quote: ... Columns were washed with 2 ml MS grade water and eicosanoids were eluted with 500 µL methanol (MeOH abs.; VWR International, Vienna, Austria) containing 2% formic acid (FA ...
-
bioRxiv - Biophysics 2022Quote: ... Cells were trypsinized and seeded on the substrate at a density of 30,000 cells cm-2 in a glass-bottom microscopy dish (VWR, 734-2906H, Barcelona, Spain) in chondrogenesis-inducing medium (ATCC ...
-
bioRxiv - Biophysics 2022Quote: Before MS analysis, peptides were resuspended in loading buffer (2% ACN, 0.1% FA) and completely solubilized in a bath sonicator (VWR, 35 kHz, 10 min).
-
bioRxiv - Neuroscience 2023Quote: ... the substrates and products were visualized under a UV lamp at 254 nm and placed separately in vials in 2 ml of Scintran (VWR, Radnor, PA, USA). The radioactivity in disintegrations per minute (dpm ...