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Citations for VWR :
1851 - 1900 of 1957 citations for 8 BROMO 4 METHYLTHIO 7 PHENYLPYRAZOLO 1 5 A 1 3 5 TRIAZIN 2 AMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... The mixture was then incubated at 4°C for 30 minutes with agitation at 1500 rpm using a thermomixer (VWR, Thermal Shake lite).
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bioRxiv - Neuroscience 2023Quote: ... and cells were incubated with primary antibodies against the pre- and post-synaptic markers of interest overnight at 4°C in antibody incubation buffer (150 mM NaCl, 50 mM Tris-Base (VWR, Cat# 101174-856), 1% BSA ...
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bioRxiv - Neuroscience 2024Quote: ... SH-SY5Y cells transfected with vector-only control or A30P αSyn with and without AcNPs were fixed using a 4% paraformaldehyde (VWR, Cat# ALFAJ61899.AK) in PBS solution for 20 min and washed with PBS and 0.1% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... Escherichia coli (Migula) Castellani and Chalmers (ATCC® 35150™) was cultured overnight in 3 mL of brain heart infusion (BHI) growth medium (VWR International, LLC, 95021-488) using a shaker-incubator at 37 °C ...
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bioRxiv - Genomics 2020Quote: ... 13.4 g/ l (1.34% (w/v)) Yeast Nitrogen Base (Sunrise Science #1501-500) and 2% glycerol (VWR #AA36646-K7).
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments were separated in 2% (w/v) agarose gels and purified using the peqGOLD Gel Extraction Kit (VWR). Heat-shock transformation of chemically competent Escherichia coli DH5α cells were performed for all vectors followed by selection on LB-agar plates with ampicillin as a selection marker ...
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bioRxiv - Cell Biology 2021Quote: ... The stable cell lines were plated at 2×106cells/ 35mm cell culture dish (VWR, Radnor, PA, cat # 82050-538). After 24hrs ...
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bioRxiv - Bioengineering 2020Quote: ... MiliQ) to make 50 mL of solution and the pH was adjusted to 7.2 using 2 M NaOH (VWR). Sodium alginate (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were diluted to 2 M urea / 0.1 M NH4HCO3 with 140 μl of HPLC grade water (VWR). Digest with mass-spec grade trypsin (Serva ...
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bioRxiv - Neuroscience 2022Quote: After MRI scanning the shanks were carefully removed and brains were transferred to 2% dimethyl sulfoxide (DMSO, VWR, USA) for cryoprotection for 1-2 days prior to cryosectioning ...
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bioRxiv - Cancer Biology 2020Quote: ... which is prepared by dissolving 2% wt/vol sodium alginate (Protanal LF200S) in water and by adding 0.5mM SDS surfactant (VWR International ...
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bioRxiv - Cell Biology 2020Quote: ... Fat droplets were stained through a freshly filtered solution of 0.3 % (w/v) Oil Red O dissolved in 60% (v/v) 2-propanol (VWR). Afterwards ...
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bioRxiv - Plant Biology 2022Quote: ... The phosphoproteome samples were resolubilized in 50 µL of 0.05% TFA in H2O/ACN 98/2 (v/v) filtered through 10 kDa MWCO PES membrane spin filters (VWR). The filtrate was also transferred to HPLC vials and injected into the LC-MS/MS instrument.
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bioRxiv - Plant Biology 2022Quote: ... The reference proteome sample was resolubilized in 30 µL of 0.05% TFA in H2O/ACN 98/2 (v/v) filtered through 10 kDa MWCO PES membrane spin filters (VWR). The filtrate was transferred to HPLC vials and injected into the LC-MS/MS instrument.
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bioRxiv - Neuroscience 2024Quote: ... was added to 2ml tubes containing Precellys Steel beads (2.8 mm ‘reinforced’ 2 ml tubes, pre-filled with steel beads purchased from VWR International Ltd ...
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bioRxiv - Bioengineering 2024Quote: ... The pH was monitored starting from 6.49 and reducing to 3.02 using a digital pH meter (accuracy ± 0.1% ± 2 digits, VWR pH110, Belgium).
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bioRxiv - Neuroscience 2023Quote: ... the canary underwent intracardiac perfusion for 2 minutes with a PBS solution containing sodium nitroprusside (VWR chemicals, 10µg/ml) followed by 20 minutes with a perfusion solution containing 4% formaldehyde (Carl Roth Art.Nr ...
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bioRxiv - Neuroscience 2022Quote: ... the flattened cortex was removed from the microscope slides and left in a cryoprotective dimethyl sulfoxide solution (2% dimethyl sulfoxide, DMSO, in 0.125 M phosphate buffer; VWR) overnight ...
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bioRxiv - Microbiology 2023Quote: ... Inoculums of 100 µL were delivered by intra-gastric gavage using a 2-inch-long plastic feeding needle (VWR) connected to a 1 mL syringe (Becton Dickinson) ...
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bioRxiv - Microbiology 2023Quote: ... and adding sterile filter double distilled water (liquid medium) or autoclaved molten 2% DNA pure agarose (VWR, Radnor, PA) (agarose plates ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 50 μL vaccine comprising DNA formulated in PBS was administered using Electroporation (EP) (BTX Agile pulse, VWR). In the remaining three immunizations ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Tryptic peptides were resolubilized in 30 μl of 0.05% TFA and 2% ACN in H2O filtered through 10 kDa MWCO PES membrane spin filters (VWR) at 14,000×g for 15 min and transferred to HPLC vials.
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bioRxiv - Neuroscience 2023Quote: Primary microglial cells were seeded at a density of 2 x 105 cells on 12 mm glass coverslips (VWR) coated with poly-L-lysine (0.1 mg/mL) ...
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bioRxiv - Microbiology 2022Quote: ... One-day-old biofilms were grown on the surface of a 4-well Permanex slide (Lab-Tek® Chamber Slide™ System, VWR, Debrecen, Hungary). The preformed biofilms were washed three times with sterile physiological saline ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Viable embryos at the 2-cell stage (0.75 hours post fertilization (hpf)) were sorted into 4 replicates of 10 embryos within 24-well plates (VWR international, LLC Radnor, PA, USA). Replicates were exposed to 1 mL of vehicle (0.1% DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in normal growth medium then switched to cysteine- and methionine-free medium DMEM supplemented with 2 mM L-glutamine (VWR) and 100 µM HPG (Invitrogen) ...
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bioRxiv - Zoology 2019Quote: ... Agar-based assay plates were prepared by adding 70 μl of 2% Agar (Bacto Agar, VWR, Becton Dickinson Sparks, MD) and 70 μl of thiabendazole solution to each well ...
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bioRxiv - Bioengineering 2021Quote: ... An array of 2 mm holes was punched in the channel outlets using a biopsy punch (VWR, Cat# 21909-132). A hole was drilled on a plate lid (VWR ...
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bioRxiv - Immunology 2020Quote: ... or Fc.IL-2 for 15 min at 37°C in complete RPMI (RPMI (Hyclone) supplemented with 10% FBS (heat inactivated, VWR), L-glutamine ...
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bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int ...
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bioRxiv - Biophysics 2022Quote: ... Cells were allowed to recover for 48 hours before passage into new 10 cm plates containing DMEM media supplemented with 2 mg/ml Geneticin sulfate (G418; VWR). Following 7 days of selection ...
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bioRxiv - Bioengineering 2021Quote: ... well plates were washed two times with MilliQ H2O and then stained with 400 μL of 2% w/V alizarin red S (VWR) for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... we fixed 250 μL of cells in 1000 μL of ice-cold methanol in a 2 mL plastic tube (16466-060, VWR). For wounded cells ...
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bioRxiv - Microbiology 2021Quote: ... was added to each well of a 96-well AcroPrep filter plate with 0·2 μM wwPTFE membrane (514-1096, VWR). A100 μL of plasma sample ...
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bioRxiv - Microbiology 2020Quote: ... Transfections were conducted by electroporation using a BioRad Gene Pulser Xcell at 1.5 kV and 25 µF with 2 mm electroporation cuvettes (VWR international) in Cytomix buffer (10 mM KH2PO4/K2HPO4 (pH 7.6) ...
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bioRxiv - Genetics 2020Quote: ... HEPES pH 7.8 and 0.00004% methylene blue in autoclaved reverse osmosis water) and mounted them on coverslip bridges (2 x 22 mm square glass coverslips (16004-094, VWR) glued together on either side of a 24 x 60 mm glass cover slip (12460S ...
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bioRxiv - Neuroscience 2021Quote: ... slides were incubated with 0.25% Triton X for 20 min (in 1X TBST) and afterwards blocked with 2% normal donkey serum (NDS; VWR, USA) in 1X ...
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bioRxiv - Biochemistry 2022Quote: ... an LD50 culture was prepared by pipetting 100 μL of the diluted expression culture into each cavity of a 96-deep well plate (96 square well, 2 mL, VWR) containing 400 μL of expression media (final OD600 = 0.02) ...
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bioRxiv - Genomics 2022Quote: ... multimodal slides with leaf sections attached to the capture areas were incubated for 2 minutes at 37°C followed by a 40-minute fixation in methanol (VWR) at -20°C ...
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bioRxiv - Immunology 2022Quote: ... Biotin+ or total DCs were sorted into U bottom 96well plate containing supplemented RPMI and 2% of T-stim media (VWR). 750 naïve CD4+ OT-II CFSE-labeled T cells from the spleen were sorted into the corresponding wells ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Resistance selection using fixed concentrations of COV-siRNA1 or COV-siRNA1+2 was conducted in Vero E6 cells cultured in DMEM supplemented with 10% FBS (VWR) and 1x Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Formic acid (FA), 2-proponol, acetonitrile (ACN), and methanol (all HiPerSolv CHROMANORM, HPLC grade, BDH Prolabo) were purchased from VWR International (Helsinki ...
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bioRxiv - Plant Biology 2023Quote: ... Flash chromatography was carried out using a 2 mL glass pasteur pipette loaded with 200 mg of P60 silica gel (SiliCycle) with hexane (HPLC grade; VWR) as the initial mobile phase ...
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bioRxiv - Developmental Biology 2023Quote: ... All PCR products were separated by electrophoresis on a 2% agarose gel in TBS stained with ethidium bromide (VWR, E3050), and genotypes determined based on sizes of DNA products.
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: The brain and lung tissue were transferred to a cold room and subjected to homogenization using a 2 ml Dounce homogeniser (VWR, Tissue grinders ...
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bioRxiv - Biochemistry 2023Quote: ... were obtained from MilliporeSigma. Tris(2-carboxyethyl)phosphine (TCEP) hydrochloride (Cat. # K831, CAS: 51805-45-9) was purchased from VWR. Tris[(1-benzyl-1H-1,2,3-triazol-4- yl)methyl]amine (TBTA ...
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bioRxiv - Microbiology 2024Quote: ... The main culture was cultivated as 10x 500 mL cultures in 2 L shake flasks sealed with breathable rayon film (VWR) for reproducible aeration for 72 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... Routine culture of E.coli for cloning purposes was performed in Miller’s LB Broth (Fisher BP1426-2) in 14mL Falcon tubes (VWR 60819-761) at 37°C on a rotating drum ...
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bioRxiv - Microbiology 2023Quote: ... Lungs were transferred to a 2 mL tube pre-filled with 2.8 mm ceramic beads (VWR, Cat. No. 10158-612), flash frozen on dry ice ...