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Citations for VWR :
1501 - 1550 of 1821 citations for 8 BROMO 4 METHYLTHIO 7 PHENYLPYRAZOLO 1 5 A 1 3 5 TRIAZIN 2 AMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Individual CNSs were sonified with five 0.3 s pulses at 1 Hz using a Branson Sonifier 450 (VWR Scientific, Winchester, PA) in Laemmli buffer (Bio-Rad ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Coating was performed for 1 h with 15 μg/ml of fibronectin or with 3.5 μg/cm2 Cell-Tak (VWR, 734-1081) diluted in 0.1 M sodium bicarbonate ...
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bioRxiv - Genetics 2022Quote: ... The slide was then transferred to 37°C for 1 min before being immersed and fixated in methanol (VWR EU, 20847.307) at -20°C for 30 min ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck). For this purpose ...
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bioRxiv - Bioengineering 2022Quote: ... containing 20% FBS (Wisent, 80450, lot 115714), 1% Penicillin-Streptomycin solution (Wisent, 450-201-EL) and 2,5 ng/mL bFGF (VWR, 10821-962) in a 37°C ...
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bioRxiv - Immunology 2020Quote: ... the monocytes were harvested and the pellet resuspended in differentiation media (RPMI-1640 supplemented with 1% FBS H.I., Penicillin/Streptomycin (P/S, VWR, Radnor, PA) and 100 ng/mL phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Genomics 2020Quote: Single cell suspensions in PBS with 2% FBS were stained by incubating for 15 minutes at room temperature protected from light with anti-human PTPRC (CD45) monoclonal antibody conjugated to FITC (1:200 dilution, VWR #ABNOMAB12230), anti-human EPCAM antibody conjugated to PE (1:50 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in 20 µL of [1 mg/mL] 100T zymolyase (cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR) and incubated at room temperature for 6-7 minutes ...
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bioRxiv - Biophysics 2023Quote: ... The cell constants for individual electrode devices and buffer conductivities were measured using a lock-in amplifier as previously described and a 500 μS cm−1 conductivity standard (VWR, UK).14 The electrophoresis data was analysed using software written in Python.17 Linear fits to the slope of the plots of velocity against electric field ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mL of supernatant was transferred to a borosilicate glass culture tube pre-cleaned with HPLC-grade methanol (VWR International). Then 1mL of EA was added to the remaining sample (to maximize extraction efficiency) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cells were then washed with 25 mL of ice cold STM buffer (pH = 7.5, 270 mM sucrose, Millipore Sigma, 1.07651.5000, 10 mM Tris HCl, Alfa Aesar, J67233, 1 mM MgCl2, VWR, E525-100ML) and spun down again at 4°C for 5 minutes at 1500 x g ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were visualised on a 1% agarose gel (run for 30 minutes at 135 V) with GelGreen (VWR, 730-1535) using an ImageQuant™ LAS 500 (GE Lifesciences) ...
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bioRxiv - Biophysics 2023Quote: ... and 1%(v/v) ammonium persulfate (APS) for 60 minutes at RT on plasma cleaned glass cover slips (No.1, 25mm Ø, VWR) that were pre-treated with 3-aminoproyltriethoxysiliane (APTES ...
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bioRxiv - Genetics 2023Quote: ... hTERT RPE-1 cells (ATCC, CRL-4000) were maintained in DMEM:F-12 media (ATCC, 30-2006) supplemented with 10% FBS (VWR, 97068-091) and 1% Pen-Strep (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... then incubated on rollers at room temperature for 1 hour with an anti-mouse secondary antibody conjugated to horseradish peroxidase (VWR, NXA931). After four further washes for 10 min each (2 x blocking buffer ...
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bioRxiv - Biophysics 2022Quote: ... Cell aggregates on top of the droplets were transferred to an electroporation cuvette (4 mm gap, VWR). Three square wave pulses of 1 kV for 70 μs were applied with 1 s intervals between each pulse using an ECM 2001 Electro Cell Manipulator (Harvard Apparatus ...
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bioRxiv - Microbiology 2019Quote: ... The antioxidant solution was prepared as follow: a stock solution containing 4% ascorbic acid (VWR, Leuven, Belgium), 0.4% glutathione and 2% Uric Acid (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: ... and lungs were excised en-bloc and fixed in 4% formaldehyde (v/v in PBS) (VWR, UK) for 2 hrs at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... in 1X PBS at 4°C before embedding them in Optimal Cutting Temperature (OCT) Compound (VWR Chemicals) and snap freezing them with isopentane on dry ice ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 48-hour incubation at 4°C in 50% optimal cutting temperature (OCT; VWR, #C361603E)/30% sucrose/phosphate buffer solution prior to freezing in 100% OCT using liquid nitrogen-cooled 2-methylbutane (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in cold PBS/0.04% BSA and fixed by adding 4 volumes 100% Methanol (VWR) and incubation for 30 min at -20 °C ...
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bioRxiv - Bioengineering 2022Quote: ... VWR Spinbar Micro Stir Bars and 4-ml Amber Borosilicate Glass sample vials were purchased from VWR.
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bioRxiv - Systems Biology 2023Quote: ... The inoculum was then streaked onto XLT-4 containing 64 µg/mL nalidixic acid (VWR; Lot:21J285302) and incubated overnight at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed using 4% paraformaldehyde for 10 minutes on the rocker (VWR analog rocker 10127-876). Fixed cells were washes once with PBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Organoids were fixed in 4% PFA and embedded in Tissue-Tek® O.C.T (VWR, cat # 25608-930). Slides of cryosectioned organoids were blocked in 5% normal donkey serum (Jackson Immuno Research ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a centrifugation (10 minutes, 16.100 x g, 4°C, MicroStar 17R centrifuge, VWR, Darmstadt, Germany) for sample clearance ...
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bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
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bioRxiv - Synthetic Biology 2022Quote: Single colonies were picked and cultured for 24 hours at 30 °C in 3 mL YPD liquid medium supplemented with appropriate antibiotics in 14 mL culture tubes (VWR) as seed culture ...
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bioRxiv - Bioengineering 2021Quote: ... formulations were aliquoted 150 μL per well (n = 3/group) in a clear 96-well plate and sealed with optically clear and thermally stable seal (VWR). The plate was incubated in a microplate reader (BioTek SynergyH1 microplate reader ...
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bioRxiv - Microbiology 2020Quote: ... and 3 μL of the culture was inoculated into 3 mL of MSgg liquid media in a 6-well or 12-well microtiter plate (VWR). The plates were incubated at 30°C for 2-3 days ...
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bioRxiv - Microbiology 2020Quote: ... or 0.4 µM (cesA) of each primer (Table 3) and 60 ng of DNA (measured by a MySpec spectrophotometer, VWR International). qPCR reaction conditions for the panC detection followed the master mix supplier’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed with PBS 3 times and fixed and permeabilized with 3.7% formaldehyde and 0.3% Triton-X (VWR 80503-490) in PBS for 30 minutes at room temperature ...
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bioRxiv - Genetics 2022Quote: ... Pre-denaturation was performed at 91°C for 3 min and then 60°C for 20 min on heat blocks (VWR). After plates were spun ...
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bioRxiv - Genetics 2022Quote: ... Predenaturation was performed at 91 °C for 3 minutes and then 60 °C for 20 minutes on heat blocks (VWR). After plates were spun ...
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bioRxiv - Immunology 2020Quote: Wild-type (WT) and TLR4 knockout (KO) BMDMs were thawed and cultured for 3 days in non-Stissue culture treated dishes (VWR) in RPMI 1640 medium with L-Glutamine containing 10 % FBS and 10 % CM (= complete medium) ...
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bioRxiv - Physiology 2021Quote: ... 200 µl of these fixed RBCs were re-suspended in a blocking buffer containing 3% bovine serum albumin (VWR 0332) and 1% normal goat serum (Lampire Biological Laboratories 7332500 ...
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bioRxiv - Bioengineering 2022Quote: ... formulations were aliquoted 150 μL per well (n = 3/group) in a clear 96-well plate and sealed with optically clear and thermally stable seal (VWR). The plate was incubated in a microplate reader (BioTek SynergyH1 microplate reader ...
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bioRxiv - Genetics 2022Quote: ... A nonspecific antibody binding was prevented by an incubation of the fixed embryos in 3% BSA (VWR Life Science, 422351S) in PBS-T (1h ...
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bioRxiv - Developmental Biology 2023Quote: ... we aspirated ovarian follicles 3-8mm in diameter using an 18g needle (Single-Use Needles BD Medical, VWR, Philadelphia, PA) connected to a regulated vacuum system and collection bottle containing oocyte collection medium (OCM ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips coated with 0.1% gelatin were fixed for 20 minutes in 3% paraformaldehyde (VWR, Radnor, PA) (resuspended in PBS and brought to pH 8.0 with NaOH) ...
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bioRxiv - Molecular Biology 2023Quote: Monocytes were isolated as described above and 3×106 cells/ml were plated in a 6-well plate using RPMI-1640 medium (#392-0427, VWR) supplemented with 10% FBS (#11573397 ...
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bioRxiv - Cancer Biology 2023Quote: ... the upper part of the membrane was cleaned with cotton swab and lower part was stained with DAPI and the intensity of DAPI was measured using FlexStation 3 Multimode Plate Reader (VWR).
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bioRxiv - Bioengineering 2023Quote: ... 3% (w/v) alginate in in serum-free DMEM)and containing cells was placed in a Luerlock syringe (VWR, LOCA120006IM). 488mM calcium sulfate in serum-free DMEM was loaded into another Luerlock syringe and mixed with the cell-alginate solution through a Luerlock connector ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and was subsequently propagated in a well of an unshaken flat-bottom polypropylene 96-well plate in one of three conditions: YPD (1% Bacto yeast extract (VWR #90000–726), 2% Bacto peptone (VWR #90000–368) ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... we plated a 150 μL of 1:100 diluted saturated culture of a sensitive strain (YAN563) on a single-well (VWR #46600-638) methylene blue agar plate (20 g/L peptone ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated with chemiluminesence substrate (Perkin-Elmer: NEL105001EA) for 1 min and signal was developed on GE Amersham Hyperfilm ECL (VWR: 95017-661). Membranes were stripped by 2 x 7 min incubations in stripping buffer (6 M guanidine hydrochloride (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... The films were rinsed in dH2O and covered with large coverslips (80 × 120 mm, thickness no.1, VWR International AS, Oslo, Norway). Imaging was performed with an epi-fluorescence microscope (Olympus BX51) ...
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bioRxiv - Biophysics 2022Quote: ... the surface of the glass coverslip serving as chamber bottom was incubated with 1% (10 mg/ml) bovine serum albumin (BSA; VWR, Radnor, PA) in phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2019Quote: ... 2-3 × 105 single cells EpiSCs were seeded on 5 μg/10 cm2 fibronectin-coated 6-well plates in media containing (1 μl/ml) ROCK inhibitor (InSolution™ Y-276320, Cat.#688001-500UG, VWR Calbiochem). In the morning of transfection ...
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bioRxiv - Microbiology 2020Quote: ... each well was washed three times with warm PBS and then treated with 500ul PBS with 1% Triton X-100 (AMRESCO, VWR, Randor PA) for 5 minutes ...