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Citations for VWR :
1451 - 1500 of 1568 citations for Ciprofloxacin Hcl 100 Ug Ml In Methanol 2 3 Carboxyl 13C3 99%; Quinoline 15N 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] solution (0.5 mg XTT/mL 1× PBS with 25 µM menadione) (XTT sodium salt, VWR) was added at 150 µl per well and incubated at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... VRE colonization levels were monitored by plating fresh stool that was serially diluted in PBS on Enterococcus selective Bile Esculin Azide Agar (BEA) media (Remel) Supplementary Fig.ed with vancomycin 6 μg/mL (VWR), erythromycin 4 μg/mL (Sigma Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... Cell pellets were diluted in 1 mL PBS and they or BALF were shaken in a glass vial (VWR) with 2 mL chloroform and 1 mL methanol containing internal standards (13C16-palmitic acid ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 mL of seed culture was used to inoculate 1 L media in a Fernbach flask (VWR 29171-854). Cells were incubated at 37 °C shaking at 210 rev/min ...
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bioRxiv - Microbiology 2023Quote: ... After spinning the aqueous phase was transferred to 1000 µL binding buffer and 10 µL magnetic beads in a round bottom molecular grade 2.2 mL 96 deep wells plate (VWR international ...
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bioRxiv - Microbiology 2023Quote: ... afra leaf material was ground and extracted in dichloromethane (DCM) (1 g:20 mL, VWR International, Radnor, PA, USA) under sonication for 30 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 500 µL of the upper phase was transferred in a new tube containing 1 mL isopropanol (VWR, 84881-320) and 2 µL f 15 mg/mL Glycoblue (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: 100 μl of cell suspension at OD 0.7 were added to 1 ml of fresh GCB medium in a 6-well plate (VWR) and incubated at 37 °C with 5% CO2 for 3 hours ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was immediately and gently mixed with 1 ml NCM medium containing 0.38 M mannitol and recovered in a round bottom tube (VWR) at 37 °C on a rotary shaker (120 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... Eluted fractions were pooled and further purified on a 1 ml HiTrap FF Q column (VWR 17-5053-01) using an AKTA FPLC system (Cytiva) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The 1.5 mL-Eppendorf tube containing the bead-PUREfrex mixture was next gently rotated on an automatic tube rotator (VWR) at 4 °C along its axis for 30 minutes for uniform liposome swelling ...
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bioRxiv - Biophysics 2021Quote: ... the lipid mixture was resuspended in 1 ml of PBS and sonicated for 10 minutes (ultrasound bath USC500TH, VWR, England) to form small unilamellar vesicles (SUVs) ...
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bioRxiv - Biophysics 2021Quote: ... The strains were first passed from agar plates (stored at 4oC) to 5 ml medium (round bottom polystyrene tubes, VWR) until early stationary phase ...
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bioRxiv - Immunology 2021Quote: ... 50 μL of mAbs diluted to 20 μg/mL in PBS were added to the first well of a 96-well U-bottom plate (VWR), and diluted two-fold in PBS for 25 μL mAb total per dilution ...
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bioRxiv - Microbiology 2019Quote: Colony morphology agar was prepared as described above and 4 mL of the mixture were poured into 35 mm×10 mm round Petri dishes (25373-041, VWR)and left to cool and solidify overnight (14 to 24 h ...
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bioRxiv - Biochemistry 2022Quote: ... The 500 mL culture (OD550 ∼0.6) was then transferred into the carboy via sterile rubber transfer line (VWR-62993-726), 18-gauge needles (Becton Dickinson 305196 ...
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bioRxiv - Genomics 2020Quote: ... Cells expressing Cas9 were selected either by sorting for GFP10 or selection with 350 ng/ml Puromycin dihydrochloride (VWR J593) for three consecutive days starting one day after electroporation17 ...
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bioRxiv - Biochemistry 2019Quote: HDL samples prepared by adding 15 μL 3 mg/mL spin-labeled apoA-I probe to 45 μL 1 mg/mL HDL and drawn into an EPR-compatible borosilicate capillary tube (VWR) (32) ...
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bioRxiv - Bioengineering 2020Quote: ... ⅘ of the 40% layer and ⅕ of the 54% layer were extracted from the polyallomer Quick-seal ultracentrifuge tube (Fisher) with an 18-gauge needle (Fisher) attached to a 10-mL syringe (VWR). The collected virus fraction was diluted in an equal volume of PBS + 0.001% Tween-20 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell suspension was filtered through a 35µm cell strainer into a 5 ml falcon round bottom tube (VWR #21008-948) for sorting using a MoFlo XDP cell sorter fitted with a 100µm nozzle ...
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bioRxiv - Bioengineering 2020Quote: ... either D-luciferin (Fisher #LUCK-2G; 150 µg / mL read concentration) or Rluc substrate (VWR # PAP1232; 17 µM read concentration). Maximum cytotoxicity was defined as luminescent signal from wells containing only media while no cytotoxicity was defined by wells containing only target cells ...
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bioRxiv - Biophysics 2020Quote: ... the diluted protein was loaded to BSA (200 μl of 1 mg/ml for 30 seconds) treated coverslips (no. 1.5H precision cover slides, VWR Marienfeld), minimizing fluorophore interactions with the glass slide ...
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bioRxiv - Cell Biology 2021Quote: 3,000 ESCs/well mixed with 0.3 μg Wnt3a-beads/well were seeded onto human fibronectin (10 μg/mL, cat. um. 7340101, VWR/SLS) coated 8-well slides (cat ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Flies were then returned to the fridge at 11 °C in individual containers (OD: 33mm, 60 ml, VWR international, USA) with 50 µl of distilled water on piece of filter paper.
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bioRxiv - Immunology 2022Quote: ... mAbs diluted to 50 μg/mL in PBS were added to the first well of a 96-well U-bottom plate (VWR), and diluted five-fold in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... overnight media was replaced either with EtBr-containing media (DMEM + 10% FBS + 0.2 μg/ml EtBr (VWR life science, X328) + 50 μg/ml uridine ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then subcultured 1:1000 into 7 mL of MSgg in a 6-well polyvinyl plate (VWR, PA, USA). Plates were incubated in static conditions at 30°C for 3 days and were then imaged using a Leica MZ10F macroscope and Leica DMC2900 camera ...
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bioRxiv - Neuroscience 2022Quote: ... clear-bottomed, 96-well plates (Falcon, Switzerland) at a density of 200,000 cells/mL or onto coverslips (CS) (VWR, Switzerland) at a density of 250,000 cells/mL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... yeast cultures were diluted to OD600=0.67 in 5 mL SGDCAA (6.7 g/L YNB without amino acid VWR #90004-150), 5 g/L ammonium sulfate (Sigma-Aldrich #A4418) ...
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bioRxiv - Biochemistry 2019Quote: ... microalgae were harvested via centrifugation at 13G (9000 rpm) for 20 mins 4°C using sterile 250 ml centrifuge tubes (VWR) and concentrated in a 50 ml sterile tubes (VWR ...
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bioRxiv - Immunology 2022Quote: Cryopreserved PBMCs were thawed and washed in RPMI before resuspension in buffer (0.2% FBS in PBS) at concentration of 1 × 106 cells/ml in FACS tubes (Falcon® Brand, VWR). Before addition of the cocktail of 21 monoclonal antibodies ...
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bioRxiv - Microbiology 2022Quote: ... All discs were subsequently placed into 1 mL of neutralising buffer (Becton Dickinson, Detroit, MI, USA) and vortexed with glass beads (2.5 - 3.5 mm; VWR, Leicestershire, UK) for 1 min to disrupt the biofilm ...
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bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated overnight at 23°C in HL-5 medium supplemented with 200 µM sodium palmitate ...
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bioRxiv - Microbiology 2022Quote: ... albicans were cultured at 37 °C with aeration in 5 mL of YPD (10g/L Yeast Extract (VWR J850-500G), 20g/L Peptone (VWR J636-500G) ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended with base HdB-Tyl and used as inoculum for new cultures in a total volume of 40 mL in 250 mL Erlenmeyer flasks (50 mm Ø) with starting OD600 of 0.1 and closed with breathable rayon film (VWR). The cultures were monitored using the Cell Growth Quantifier (Aquila Biolabs ...
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bioRxiv - Genomics 2023Quote: ... 5000 cfu were plated in 1 mL media in each of 21 wells of a 24-well culture plate (10861-558, VWR), and initial cultures were plated for cfu on 7H10 + OADC to confirm number of cells in initial culture ...
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bioRxiv - Microbiology 2023Quote: ... The lentivirus-containing medium was then removed and cells were allowed to recover for 72 hours whereupon cells were passaged in the presence of 1 μg/mL puromycin (VWR) and remained under selection for 3 days.
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bioRxiv - Developmental Biology 2023Quote: ... Egg chambers were dissected and manually dechorionated in isolation buffer and then incubated for 30 min in Zalokar’s buffer containing 4.5% hydrogen peroxide and 1 mg/ml horseradish peroxidase (VWR, Radnor, PA) before bleaching as above.
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bioRxiv - Immunology 2023Quote: ... Monocytes were enriched by negative selection using StemCell RosetteSep Human Monocyte Enrichment Cocktail (StemCell 15068; 75μl per ml of blood) and Ficoll-Paque (VWR 17144003). Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034 ...
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bioRxiv - Synthetic Biology 2022Quote: ... SbU- +bts1Δ and SbU- +bts1Δ + thi6Δ strains were incubated in 2.6 mL YPD in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen ...
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bioRxiv - Microbiology 2023Quote: ... PBS was added to wells (1 mL) and the base and sides of each well were scraped twice using a cell scraper (VWR) to disrupt biofilm ...
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bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 amoebae were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated at 23°C in HL-5 medium ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed and 10 ml of serum-free DMEM containing 0.2% w/v bovine serum albumin (BSA; VWR international) and 1 μg/ml L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transformed with gene-specific linear DNA and selected on Kanamycin (50 µg/mL) (USP, VWR Life Science, USA) plates for gene deletion ...
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bioRxiv - Genomics 2023Quote: ... followed by trituration of brains in 5 ml of DMEM complete media using a glass Pasteur pipette (VWR, 14672-380). The triturated tissue was resuspended of DMEM complete media and filtered through a 40 μm nylon cell strainer (Corning ...
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bioRxiv - Developmental Biology 2023Quote: ... Bulk samples including bone and bone marrow were cryo-pulverized and resuspended in 1 ml ice-cold TriFast (VWR International) and carefully vortexed (30 sec) ...
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bioRxiv - Systems Biology 2023Quote: ... The metabolite extracts were transferred into 0.3 mL HPLC autosampler vials with inserts (VWR International LLC, Cat. 9532S-1CP-RS) and stored in −80°C until analysis.
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bioRxiv - Microbiology 2023Quote: ... Then 20 μL of hemolymph per each sample were digested with 0.5 mL of 32% ultrapure hydrochloric acid (VWR Chemicals) under heating conditions (60 °C ...
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bioRxiv - Synthetic Biology 2024Quote: The variant BUD-ELMs were grown in standard conditions and then collected into a 1.5 ml Eppendorf tube and spun for 10 s at 3200 rcf using a mini centrifuge (VWR - C0803). Then the supernatant was removed ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5M EDTA) and lysed in SEMZ buffer (1M Sorbitol, 50mM EDTA, 28mM β-Mercaptoethanol and 2mg/ml Zymolyase 100T (IC320932 VWR)) ...