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Citations for VWR :
1201 - 1250 of 1445 citations for Ciprofloxacin Hcl 100 Ug Ml In Methanol 2 3 Carboxyl 13C3 99%; Quinoline 15N 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 2-2.5 × 106 frozen or freshly isolated monocytes were seeded in 10 cm non-tissue culture treated dishes (VWR, #734-2796) in 10 mL RPMI 1640 (Biowest ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% dextrose [VWR #90000– 904] ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
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bioRxiv - Microbiology 2023Quote: ... A total of 20 μg of proteins were mixed with 6x SDS loading dye containing 10% 2-mercaptoethanol (VWR Life Sciences) and boiled at 100 °C for 10 min before loading ...
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bioRxiv - Evolutionary Biology 2021Quote: ... dried extracts were reconstituted in 30 μl of 11.4 mg/ml MOC in anhydrous pyridine (VWR), vortexed for 10 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Completed DMEM was added to inactivate trypsin and cells were collected into 15 mL tubes (VWR). The tubes were centrifuged at 300 rcf for 5 minutes to pellet cells and total protein was extracted by resuspending each pellet in 1 mL of RIPA buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Trypsin was then quenched by adding 4 mL MEF media (Dulbecco’s Modified Eagle Medium (DMEM, VWR) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... medium was replaced by 60 μL of 5 mg/mL Collagen Type I (VWR, 734-1085) in 1M NaOH and 1X DMEM/F12 ...
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bioRxiv - Pathology 2022Quote: ... dried extracts were reconstituted in 30 μL of 11.4 mg/ml MOC in anhydrous pyridine (VWR), vortexed for 10 min ...
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bioRxiv - Bioengineering 2022Quote: ... 350μL of a 40mg/mL−1 solution of bovine serum albumin (BSA) (AAJ64100, VWR, PA, USA) in PBS (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... Five ml of each strain suspension were distributed in 6-well plates (VWR tissue culture plate) containing a sterile borosilicate coverslip in the bottom ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Cells expressing Cas9 were selected by 350 ng/ml Puromycin dihydro-chloride (VWR International GmbH, #J593) for three consecutive days starting one day after electroporation (Steyer et al. ...
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bioRxiv - Biophysics 2021Quote: Peripheral blood monocytes were isolated from human blood using 15 Ml Ficoll-Paque (VWR, Darmstadt, Germany) centrifugation gradient ...
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bioRxiv - Molecular Biology 2022Quote: ... We then transferred the column to a labeled DNA LoBind Eppendorf 1.5 mL tube (VWR # 022431021), added 20 uL nuclease-free H2O ...
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bioRxiv - Evolutionary Biology 2019Quote: 1 ml of each lysate was first treated with 12.5 μl 1M MgCl2 (VWR Life Science), 0.4 μl DNAse 1 (2000 U/ml ...
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bioRxiv - Plant Biology 2019Quote: ... weighed tissue samples were digested in 2.5 ml of concentrated nitric acid (AR Select Grade, VWR) with an added internal standard (20 p.p.b ...
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bioRxiv - Bioengineering 2020Quote: ... We re-suspended the phage in 10-mL Tris buffer (10 mM, pH 8.5, VWR international), and added 10 ml of a solution containing 0.2 M NaOH (VWR international ...
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bioRxiv - Cell Biology 2021Quote: ... Dissociated cells were harvested and washed with Wash medium (DMEM, 0.1% BSA) + 1mg/ml DNase (VWR) twice and centrifuged at 300 rcf for 3 mins ...
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bioRxiv - Microbiology 2021Quote: ... 4.5 ml of the supernatant was sterile filtered using 0.2 µm syringe filter (VWR 28145-501). 5 x 108 cells of luminescent E ...
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bioRxiv - Molecular Biology 2021Quote: ... rose tissue was macerated in 1 mL of phosphate buffer saline (1X PBS) (VWR, Radnor, PA), 0.05% Tween 20 ...
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bioRxiv - Microbiology 2022Quote: ... 200 μL of each overnight culture was resuspended in 20 mL of Marine Broth 2216 (VWR) in an Erlenmeyer flask in shaking incubator at 220 rpm for 3 h until each species reached an optical density of 0.15 ...
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bioRxiv - Microbiology 2022Quote: ... We filter-sterilized the media using 150-mL 4-μm vacuum filtration tops (VWR: 10040-444) after adjustment ...
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bioRxiv - Evolutionary Biology 2022Quote: ... EBY100 cells were thawed by inoculating 5 mL YPD (1% Bacto yeast extract (VWR #90000-726), 2% Bacto peptone (VWR #90000-368) ...
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bioRxiv - Microbiology 2022Quote: ... and supplemented with 10% sheep defibrinated blood (Hemostat) with 20 μg/mL streptomycin (VWR Life sciences) or classical LB broth (Difco ...
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bioRxiv - Synthetic Biology 2023Quote: ... then plated onto YPM supplemented with 60 µg mL-1 5-fluorocytosine (5-FC) (TCF0321, VWR). Colonies that emerged were then transferred onto a new YPM 5-FC agar plate for clonal isolation ...
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bioRxiv - Biochemistry 2023Quote: ... dried extracts were reconstituted in 30 μL of 11.4 mg/ml MOX in anhydrous pyridine (VWR), vortexed for 10 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... SPE columns were washed with icecold H2 O (5 mL, MS grade, VWR International, Vienna, Austria) and elution of analytes was carried out with ice-cold MeOH (500 μL ...
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bioRxiv - Evolutionary Biology 2023Quote: ... “deep well plates” are always 96-well 2.2 ml polypropylene deep well plates (VWR International, USA). Any 4°C storage was also in the dark.
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bioRxiv - Microbiology 2023Quote: ... Individual colonies from plates were inoculated into 5 ml of tryptic soy broth without dextrose (VWR) and shaken for 8 hours at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... and the wells were stained with 0.5 mL/well of 1x Crystal Violet (VWR, 470337-534) for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Pellet was resuspended in 1 mL of Dulbelcco’s Modified Eagle’s Medium (DMEM) (Corning; VWR 45000-304). This suspension was diluted 10-fold by adding 500 uL to 4.5 mL of DMEM to a final concentration of 1×106 cells/mL ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were harvested 24 h later using 1 mL TriFast™ (VWR, cat. no. 30-2010) per well ...
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bioRxiv - Biochemistry 2024Quote: ... The digested samples were transferred to 15 mL metal-free polypropylene vials (VWR, Randor, PA, USA) and diluted with ultrapure water to achieve 0.6 M final acid concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... cells expressing the various shRNAs were selected with 0.2 mg/ml hygromycin (cat#K547-20ml, VWR) at 72-hours post transfection and maintained at 0.8 mg/ml of hygromycin after cell line purification.
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bioRxiv - Immunology 2022Quote: Splenic T-lymphocytes were counted and plated at an optimal seeding density of 8×104 cells per 100 µL in 96 well Microtitration ImageLock plates (VWR, #4379, Radnor, PA, USA). The cells were cultured with anti-CD3 and anti-CD28 activation beads and imaged over the course of 96hrs in the Essen Bioscience IncuCyte S3 Live-Cell imager and analysis system.
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bioRxiv - Bioengineering 2021Quote: ... The cells were plated to ∼2 x 105 cells per well in a 6-well VWR tissue culture-treated plates (VWR, Suwanee, GA) and incubated ∼12 hours (overnight) ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cell Biology 2021Quote: ... the eye cups were infiltrated with a 2:1 mixture of PBS containing 30% sucrose and OCT compound (VWR International, Radnor, PA) and frozen with dry ice ...
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bioRxiv - Biophysics 2021Quote: ... and subsequently incubated at 37 °C and 5% CO2 for 2 h before seeded and imaged on an 18 mm diameter microscope cover glass (VWR, Darmstadt, Germany) in PBS containing Ca2+ (Gibco ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Transformants were plated on SDCAA-agar (1.71 g/L YNB without amino acids and ammonium sulfate [Sigma-Aldrich #Y1251], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% dextrose [VWR #90000–904] ...
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated with secondary antibodies and DAPI for 2 hr at room temperature prior to mounting in AquaPoly/Mount mounting medium (VWR, 87001-902).
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bioRxiv - Neuroscience 2019Quote: ... the flies were first sated with water and then presented with 100mM sucrose containing 2% blue dye (McCormick) filled in a graduated capillary (VWR, #53432-604). Until the flies became unresponsive to 10 serial food stimuli ...
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bioRxiv - Microbiology 2022Quote: ... Spleen and brain were homogenized in 1 ml of sterile PBS followed by culture of 10-fold dilutions of each tissue on 2X GYE agar (2% glucose (VWR™, USA), 1% yeast extract (BD™ ...
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bioRxiv - Immunology 2023Quote: ... mice were whole body perfused through the heart with 2% paraformaldehyde (diluted down in PBS from 16%; VWR Cat. No. 15710-S), then livers were harvested and placed in 2% paraformaldehyde for 24 hours at 4°C then placed in 30% sucrose (Sigma-Aldrich Cat ...
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bioRxiv - Physiology 2023Quote: ... after overnight fasting followed by administration of a saline solution of glucose (2 g/kg or 1.5g/kg, for rats or mice, respectively; VWR Chemicals, Leuven, Belgium), by gavage ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed 3x for 15 minutes with PBS-T (PBS with 0.02% Tween) and incubated for 2 hours with either anti-rabbit HRP-linked (VWR, cat. no NA934) or anti-mouse HRP-linked (VWR ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Equal volumes (5 μL) of the resulting PCR product were loaded onto a 2% biotechnology grade Agarose I (VWR International, PA, USA) gel prepared with 1X TBE buffer and 0.0025% ethidium bromide ...
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bioRxiv - Neuroscience 2024Quote: ... mice were acclimated in a custom clear acrylic behavioral chamber (4” x 2” x 6”) with a filter paper bottom (VWR #28321-113) for 10 minutes prior to intranasal treatment ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown at 21°C in shaking culture in HL5 medium (Formedium, Norfolk, England) and on SM/5 agar plates (2 g/L glucose (VWR, Solon, OH), 2 g/L bacto peptone (Becton ...