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Citations for VWR :
1401 - 1445 of 1445 citations for Ciprofloxacin Hcl 100 Ug Ml In Methanol 2 3 Carboxyl 13C3 99%; Quinoline 15N 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... the surface of the glass coverslip serving as chamber bottom was incubated with 1% (10 mg/ml) bovine serum albumin (BSA; VWR, Radnor, PA) in phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2019Quote: ... 2-3 × 105 single cells EpiSCs were seeded on 5 μg/10 cm2 fibronectin-coated 6-well plates in media containing (1 μl/ml) ROCK inhibitor (InSolution™ Y-276320, Cat.#688001-500UG, VWR Calbiochem). In the morning of transfection ...
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bioRxiv - Bioengineering 2022Quote: ... pCD microparticles were suspended in 10 mg/mL of DOX in DMSO and incubated on an end over end mixer (VWR, Radnor, PA) for 3 days at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was changed and the incubation continued for another 24 hours before addition of 1-5 µg/mL puromycin (VWR 97064-280). Incubation with puromycin continued for several days until untransfected control wells were depleted of cells ...
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bioRxiv - Biophysics 2022Quote: ... as diluent and were filtered through a 0.1 µm PES filter mounted onto a 50 mL centrifuge tube (#514-0366, VWR, Radnor, PA, USA) before use ...
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bioRxiv - Cancer Biology 2022Quote: Splenocytes were isolated from the spleen of 6-10-week-old OT-I male mice and pulsed with 2ug/ml of OVA peptide SIINFEKL (VWR, H-4866.0001BA) for 4 h in T cell culture media composed of RPMI1640 (GE Healthcare ...
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bioRxiv - Genomics 2021Quote: ... and myotubes from one 10 cm plate (∼5 million cells) with >90% viability were lifted as described above and pelleted in 15 mL polypropylene falcon tubes (VWR, 89039-670) by centrifuging for 5 minutes at 1500 RPM ...
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bioRxiv - Cell Biology 2021Quote: Aggregation was induced by agitating 5 mg/mL α-synuclein at 37 °C for 7 days 1200 rpm (VWR, Cat#12620-942). Fibril formation was evaluated by electron microscopy (see next section for details) ...
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bioRxiv - Immunology 2020Quote: One mL of BMDM from lipin-1mKO and littermate control mice at a concentration of 1×106/mL was placed into falcon tubes (VWR-60818-500). Cells were either treated with or without IL-4 (40ng/mL ...
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bioRxiv - Bioengineering 2022Quote: ... cells for both batches were seeded onto the material at a confluence comprised between 50% and 60% on fibronectin coated (15 μg/ml in PBS buffer solution) 18-mm round glass coverslip (VWR, Radnor, USA). The cells were maintained in incubator at 37°C and 5% CO2.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then washed 3x for 5 minutes in PBS and exchanged into a permeabilization buffer consisting of PBSM + 5 mg/mL BSA (VWR, 0332-25G) + 0.1% Triton-X100 (Millipore Sigma ...
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bioRxiv - Microbiology 2022Quote: ... containing 1.5 ml of brain–heart infusion broth ([BHI]; Becton Dickinson, Franklin Lakes, NJ) supplemented with 0.1% Tween 80 (VWR Chemicals, Solon, OH) and were incubated at 37°C with 5% CO2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each fin bud was first homogenized in 50 μL of Buffer RLT (supplemented with β-mercaptoethanol per kit recommendation) for 1 min at RT in a 1.5 mL centrifuge tube using a cordless motor (VWR Catalog # 47747-370) and pestle (USA Scientific Catalog # 1415-5390) ...
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bioRxiv - Plant Biology 2023Quote: ... from accessions grown in UC Davis or UC Riverside greenhouses and floated overnight in 1 mL deionized water in a 24-well tissue culture plate (VWR #10062-896) with a plastic lid to prevent evaporation of the water ...
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bioRxiv - Cancer Biology 2023Quote: ... Each well is washed with 1 mL of phosphate buffered saline (PBS) prior to fixation with 500 µL of 10% buffered formalin (VWR, 89379-094). After at least 24 hours of fixation ...
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bioRxiv - Microbiology 2023Quote: ... The AB food consisted of standard media containing 200 mg/ mL of ampicillin and kanamycin (VWR Life Science; both dissolved in water), 50 mg/ mL of tetracycline (EMD Millipore Corp. ...
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bioRxiv - Biochemistry 2022Quote: ... from the working seed bank into high intensity perfusion CHO (HIP-CHO) medium (Thermo Fischer Scientific, Waltham, MA) into a 125 mL unbaffled shake flask (VWR, Radnor, PA) with a 40 mL working volume to a seed density of 0.5×106 cells/mL containing 0.1% anticlumping agent (Thermo Fischer Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The animals were sacrificed 72h after the Hypoxisense 680 measurement with Dolethal (Pentobarbital Sodico) (200 mg/ml, Vetoquinol, Aartselaar, Belgium) and the tumors were fixed in 4% paraformaldehyde (PFA, Klinipath, VWR, PA, USA). Only histological procedures were performed on these tumors.
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bioRxiv - Biochemistry 2023Quote: ... Waltham, MA) containing 0.1% anticlumping agent (Thermo Fischer Scientific, Waltham, MA) in a 125 mL unbaffled shake flask (VWR, Radnor, PA, USA) with a 40 mL working volume ...
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bioRxiv - Cell Biology 2023Quote: Lyophilized liver samples were homogenized in 10 mM phosphate buffer at pH 6 (28 mg tissue per ml buffer) on a bead beater (VWR, Radnor, PA) for 1 min ...
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bioRxiv - Cell Biology 2023Quote: ... washed cells were suspended at a concentration of 1 × 106 cells/mL in the growth medium and filtered using a 40 µm cell strainer (VWR: 10199- 655). Cells were then sorted into 15 mL collection tubes using a sterile 100 µm chip (Sony ...
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bioRxiv - Microbiology 2024Quote: ... 1 mL of M9 was added to the plate and the bacterial lawns were scraped with a sterile L spreader (VWR 490007-358). Bacterial slurry was pipetted and centrifuged at 13000 x g for 4 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... to get a concentration of 25 mg/mL and was then sterile-filtered using a syringe with a filter insert (VWR, Cat# CA28145-501). This solution was diluted 1000 times in culture media to achieve a final DMSO concentration that was lower than 0.1% ...
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bioRxiv - Plant Biology 2020Quote: Leaf tissue was collected 7 days post infection, transferred to an extraction bag (Bioreba, Switzerland) and mixed with 5 ml of phosphate buffered saline (VWR, West Chester, PA), hand homogenized using a hand-held tissue homogenizer (Agdia ...
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bioRxiv - Microbiology 2020Quote: ... The liquid and solid fractions of a sample were separated by filtration through a combination of three layers of sterile cheesecloth that had been wrapped separately and baked at 175°C and then transported in sealed 15 ml conical Falcon tubes (catalog number: 62406-200, VWR International, Radnor, PA) on dry ice to the laboratory and stored at −80°C ...
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bioRxiv - Developmental Biology 2021Quote: ... were prepared and stored in 0.2 mL snap strip PCR tubes with dome caps (490003-692, GeneMate – BioExpress, VWR International, Brisbane, CA, USA) at -20 °C prior to qRT-PCR plates preparation ...
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bioRxiv - Physiology 2023Quote: ... Each brain tissue was carefully collected from the cranium individually and snap-frozen in a 1.5 mL microcentrifuge tube (VWR International, Radnor, PA, USA) chilled with liquid nitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Adjusted cultures were then mixed in a 2.5:97.5 ratio of fluorescent:non-fluorescent cells and nine ml of this mixture was added to 13×100mm culture tubes (VWR, Cat no. 10545-936) and grown in 25°C incubator for three days ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were stimulated with p25 peptide-pulsed (sequence: FQDAYNAAGGHNAVF from Genescript) iBMDMs or FLAMs that were irradiated with mitomycin (25 µg/mL) (VWR, Cat no. TCM2320) and had been pre-treated with DMSO ...
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bioRxiv - Physiology 2023Quote: ... fish were acclimatized for 4–5 days prior to the assay in a cylindrical assay chamber (325 mL glass dish, 10 cm × 5 cm, VWR, Radnor, PA, USA) filled with conditioned water (pH 6.8–7.0 ...
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bioRxiv - Genetics 2023Quote: ... with a scalpel on a cold block (Figure 1b) and resuspended on ice in 1 ml of lysis buffer containing 0.32 M Sucrose (VWR Catalog No. 97061-432), 10 mM Tris-HCl pH 8 (Invitrogen REF 15568-025) ...
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bioRxiv - Neuroscience 2024Quote: ... Premixed gases were delivered into the chamber through the gas inlet at a flow rate of ∼700 mL/min (controlled by a flow meter, VWR model #FR2A138VVT-VW) from gas tanks (Airgas ...
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bioRxiv - Neuroscience 2024Quote: ... Humidified gas was delivered through one port into the chamber through flexible PVC tubing at a flow rate of 30 mL/min (controlled by a flow meter, VWR model #GR60140AVB-VW) from two gas tanks fitted with valves controlled by a ValveBank TTL pulse generator ...
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bioRxiv - Cancer Biology 2023Quote: ... Appropriate number of acinar cells were then resuspended in premade collagen solution (1 mg/ml rat tail type1 collagen (VWR, Cat. No. CACB354236), 10× Waymouth’s media (Cedarlane Labs ...
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bioRxiv - Microbiology 2024Quote: ... and the spores were resuspended in 5 ml of 50% ethanol and incubated at 4°C in a tube rotator (VWR Mini Tube Rotator) running at 15 rpm for 12 h at room temperature to inactivate remaining vegetative cells ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Single colonies were grown overnight in 10 mL YPGal+Dex and genomic DNA was isolated from 1 mL aliquots using the VWR® Life Science Yeast Genomic DNA Purification Kit (VWR 89492-616, us.vwr.com).
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bioRxiv - Physiology 2023Quote: ... 30 males and 30 females from each diet were individually placed in 3.7 mL screw-top vials in sealed plastic bags and submerged in a refrigerated circulating bath (model AP28R-30; VWR international, Radnor, PA, USA) containing a 1:1 v/v mixture of ethylene glycol and water ...
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bioRxiv - Microbiology 2023Quote: ... The suspension was allowed to cool at room temperature (RT) for 10 min before adding 1 µl RNAse at 10 mg/ml (VWR cat no. 0675-250MG) and incubation at 37 for 60 min ...
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bioRxiv - Microbiology 2023Quote: ... The cell pellets were resuspended in 480 µl 50 mM EDTA and treated with 120 µl of 20 mg/ml lyzozyme (VWR cat no. 0663-5G), incubated at 37°C for 60 min in order to lyze possible gram-positive bacteria ...
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bioRxiv - Synthetic Biology 2023Quote: ... an overnight LB culture of the indicated strain and expression plasmid was used to inoculate 20 mL of AB autoinduction media with the appropriate antibiotics in a vented baffled 250mL Erlenmeyer flasks (VWR, cat. no. 89095-270). Flasks were incubated at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... Diluted cells were plated onto fresh LB plates (15 ml medium per plate, the diameter of the plate is 10 cm and the height 15 mm, VWR, US, Catalog number 25384-342), and multiple dilutions were used ...
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bioRxiv - Cell Biology 2022Quote: ... then spheroplasted in digestion mix (8.9% glusulase [part no. NEE154001EA, PerkinElmer] and 1 mg/mL 100T zymolyase [cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR] in 1.2M sorbitol-citrate) by rotating at 30°C for 4-5 hours (digestion was assessed by looking at cells on a light microscope) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Brain slices were homogenized with 50 ul of PBS in 1.5 ml Eppendorf™ tubes using a Kontes® pellet pestle (VWR™ cat. No. KT749521-1500), followed by passaging through a 17-gauge needle attached to a 1 mL syringe ...
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bioRxiv - Microbiology 2023Quote: ... Six to seven grams (6 ml) of soil was transferred to each well of the 12-well microtiter plates (VWR American cat no.:10861-556, USA), containing three one-millimeter holes in the bottom of all wells for drainage ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were then picked and inoculated into 800 μL of M9610 liquid media with ampicillin in a loose cap culture tube (VWR, 13 mL culture tube, cat #60818-667) and grown in an air shaker (New Brunswick Scientific Excella E24 M1352-0000 ...