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Citations for VWR :
1101 - 1150 of 1155 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... 8 μl of the final polyacrylamide mix were pipetted on 35 mm glass bottom dishes (Cell E&G LLC VWR, USA) which were pretreated with 0.1 M NaOH ...
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bioRxiv - Cancer Biology 2024Quote: A total of 5 × 106 MM.1Sgfp+/luc+ cells were seeded in T-25 flasks (Avantor/VWR, Cat. No. 10861-568) and treated with the vehicle (DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentivirus-containing medium was collected from HEK293T cells 48h and 72h post-transfection and added to recipient cancer cells after filtration using a 0.45 µM filter (VWR-Corning, 431220). 48h post infection ...
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bioRxiv - Neuroscience 2024Quote: ... We obtained HEK-293 cells from Jong Yun Lab (Penn State College of Medicine) and cultured them in DMEM (VWR International) supplemented with 10% fetal bovine serum (Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... 5-day differentiated BMDMs isolated from control LysM-Cre+ or LysM-Cre+ Atg16L1loxp/loxp mice were plated at 10 x 106 cells per 15-cm non-TC treated dish (Petri dish, VWR, 25384-326) in BMDM media ...
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bioRxiv - Developmental Biology 2021Quote: ... for duck fibroblasts (i.e., CCL-141 cells) supplemented with 10% Fetal bovine serum (FBS, 97068-085, Lot# 283K18, VWR, Radnor, PA, USA) and 1X penicillin-streptomycin (15140122 ...
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bioRxiv - Biochemistry 2019Quote: ... 1 x 106 cells were pelleted and resuspended in a 1:2.5:1 mixture of H2O: methanol: chloroform (both VWR, Radnor PA, USA) pre-cooled to −20 °C ...
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bioRxiv - Systems Biology 2020Quote: ... cells were removed from their liquid media by flowing the liquid cultures that contained cells through a 0.45−μm pore filter (VWR, cellulose-acetate membrane). To ensure and verify that there were no cells remaining in the filtered media ...
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bioRxiv - Systems Biology 2020Quote: ... all subpools were combined into the final mutant collection pool by pipetting the subpools through a 100 μm cell strainer (VWR 10054-458). The final pool was mixed using a magnetic stir bar ...
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bioRxiv - Cancer Biology 2020Quote: 1.2 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cancer Biology 2020Quote: 3 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-742). The next day ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Neuroscience 2021Quote: ... After which cells were washed once with 1x PBS with 1% BSA before being filtered through 40um FlowMi Tip Strainers (VWR, 10032-802). Cells were then diluted with 1x PBS with 1% BSA and loaded onto the Chromium 10x Controller according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and myotubes from one 10 cm plate (∼5 million cells) with >90% viability were lifted as described above and pelleted in 15 mL polypropylene falcon tubes (VWR, 89039-670) by centrifuging for 5 minutes at 1500 RPM ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 2000 cells per well in a volume of 100 µl were seeded on flat-bottomed 96-well plates (VWR, Radnor, USA) and incubated in a 95% humidified atmosphere with 5% CO2 at 37 °C for 24 h ...
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bioRxiv - Developmental Biology 2020Quote: ... for duck fibroblasts (i.e., CCL-141 cells) supplemented with 10% Fetal bovine serum (FBS, 97068-085, Lot# 283K18, VWR, Radnor, PA, USA) and 1X penicillin-streptomycin (15140122 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Each plant was sprayed for one minute and the collected flow through from each plant were first strained using a 40 μm cell strainer (VWR 76327-098) to remove large microorganisms and large particles ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed twice with mitochondrial assay solution (220mM mannitol [Sigma-Aldrich, M4125], 70mM sucrose [Sigma-Aldrich, S7903], 10mM KH2PO4 [VWR, 470302] ...
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bioRxiv - Microbiology 2022Quote: ... 1 cell line was cultured at 37°C with 5% CO2 in DMEM with high glucose and L-glutamine (VWR 16777-129) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-2 preps of in vitro transcribed RNA and 5-10µg N gene RNA were mixed with the prepared cell suspension in a 4mm electroporation cuvette (VWR International # 89047-210) and pulsed in a BioRad GenePusler using the exponential protocol with the following settings ...
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bioRxiv - Cell Biology 2022Quote: The lipidomics solubilization buffer used to resuspend whole-cell lysates and GolgiTAG immunoprecipitates was made of LC/MS grade chloroform (VWR. Cat# 22711.260) and Optima LC/MS grade methanol (Fisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... when confluence was reached and counting/replating using Trypan blue exclusion dye in an automatic cell counter (EveTM, NanoEnTek distributed by VWR, Milan, Italy) in accordance with the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... After a PBS wash 500 µl TRIzol was added to the cells followed by the addition of 300 µl chloroform in a heave gel phase-lock tube (VWR, 10847-802) for phase separation using centrifugation ...
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bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were seeded at 1×106 cells/well in 24-well plates and maintained in X-VIVO-15™ media (CA12001-988, VWR, USA). Four treatment groups were used ...
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bioRxiv - Bioengineering 2024Quote: ... The cells collected from the BM and spleen were filtered through 70 μm mesh and enriched for haemopoietic lineage cells by density centrifugation using Lympholyte-Mammal (VWR, Lutterworth, UK), centrifuging the cell suspension for 20 min at 625 g at RT ...
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bioRxiv - Genomics 2024Quote: Total RNA of minigene-transfected HEK293T cells and patient-derived fibroblasts was extracted using the peqGOLD Total RNA Kit (VWR Life Science). One microgram of RNA was treated with 1 U of DNaseI (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: Concentration and quality of total RNA isolations using a phenol-chloroform extraction with Isol-RNA lysis reagent (5’Prime, Hamburg, Germany) from endothelial cells were verified by a NanoDrop™ 1000 (Peqlab VWR, Radnor, PA) and an Agilent 2100 bioanalyzer (Agilent ...
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bioRxiv - Bioengineering 2019Quote: ... and β3TUB is a tubulin thought to be specifically involved during differentiation of neuronal cell types [69].) Antibodies were diluted in antibody dilution buffer (PBS containing 1% bovine serum albumin (VWR, Radnor, PA, USA) and 0.3% Triton X-100 (Sigma-Aldrich)) ...
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bioRxiv - Biophysics 2022Quote: ... Cells were trypsinized and seeded on the substrate at a density of 30,000 cells cm-2 in a glass-bottom microscopy dish (VWR, 734-2906H, Barcelona, Spain) in chondrogenesis-inducing medium (ATCC ...
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bioRxiv - Cell Biology 2019Quote: ... Single cells were sorted into 200 μL fresh culture medium in one well of a 96-well flat bottom plate (VWR Catalog #29442-054).
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bioRxiv - Biophysics 2021Quote: ... the fluidic sample cells were constructed using two pieces of double-sided tape sandwiched between a microscope slide and glass coverslip (VWR 22×30 mm). Each microscope slide had a hole on each of two ends ...
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bioRxiv - Cell Biology 2020Quote: ... The cultivation media used for each cell line was recommended by the supplier with an addition of 10% Fetal Bovine Serum (FBS, VWR, Radnor, PA, USA). For immunostaining ...
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bioRxiv - Microbiology 2021Quote: ... Vero cells were seeded at 60% confluence in 24-well plates equipped with 13 mm round glass coverslips (VWR, cat n. 631-0150). Cells were washed once with PBS before incubation with BetaCoV/Australia/VIC01/2020 diluted in PBS at an MOI=5 ...
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bioRxiv - Immunology 2020Quote: ... Fused cells were then seeded on 96 well flat bottom plates onto mouse peritoneal cells as feeder layer in hypoxanthine-aminopterine-thymidine (VWR, Radnor, Pennsylvania, USA) medium for the selection process.
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bioRxiv - Genetics 2019Quote: We performed cell culture using the conditions described above in 12-well plates containing sterile 18-mm glass cover slips (VWR, Cat#48380-046). We fixed cells for 10 minutes in 4% paraformaldehyde (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The cell suspensions were filtered through a 35 μm nylon filter cap on 12 × 75 mm round bottom tubes (VWR/Falcon 21008-948). Flow cytometric measurements were performed using a BD LSR Fortessa ...
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bioRxiv - Genomics 2022Quote: ... HeLa S3 or HeLa S3 + TOP2A- Venus cell linescells were either grown or spun onto 22×22mm No 1.5 coverslips in 6-well plates (VWR Cat No 48366-277). (cell cycle analysis experiments ...
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bioRxiv - Microbiology 2023Quote: ... Adjusted cultures were then mixed in a 2.5:97.5 ratio of fluorescent:non-fluorescent cells and nine ml of this mixture was added to 13×100mm culture tubes (VWR, Cat no. 10545-936) and grown in 25°C incubator for three days ...
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bioRxiv - Molecular Biology 2023Quote: ... we seeded 2×104 ESCs per well of a Geltrex-coated 12-well plate for subsequent RT-PCR and RT-qPCR analyses and 1-3×103 cells per well of a 12-well plate containing a Geltrex-coated 18-mm glass coverslip (VWR, cat# 631-1580) for immunostaining ...
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bioRxiv - Bioengineering 2024Quote: ... PCLS were then placed on Gelfoam® for long-term culture and monitored using a WST-8 cell counting kit (VWR #89155-898) according to the kit’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Appropriate number of acinar cells were then resuspended in premade collagen solution (1 mg/ml rat tail type1 collagen (VWR, Cat. No. CACB354236), 10× Waymouth’s media (Cedarlane Labs ...
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bioRxiv - Cancer Biology 2024Quote: 5×106 MM.1Sgfp+/luc+ cells were treated with DMSO or 1.00 μM TriC for 24 h in T-25 flasks (Avantor/VWR; Cat. no. 10861-568). Cells were then harvested ...
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bioRxiv - Bioengineering 2019Quote: ... The Jurkat cell line (Clone E61, ATCC® TIB 152™) was obtained from ATCC and maintained in Complete RPMI medium: RPMI 1640 (VWR, Radnor, PA) + 25 mM dextrose ...
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bioRxiv - Cancer Biology 2020Quote: Primary OSE cultures and the OSN2 cell line (Flesken-Nikitin et al. 2003) were maintained in culture in media containing DMEM (VWR; Cat#: 10-017-CM), Hams F12 (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... was sterilized overnight again before being was sonicated in a Vibra Cell VC 750 sonicator equipped with a Cup Horn probe (VWR, Fontenay-sous-Bois, France) with the following settings ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μL cell suspension was loaded on YES agarose pad (2% agarose) and covered with 22 x 22 mm glass coverslip (VWR #631-0125, thickness: 1.5). Time-lapse imaging of mNG-cdc8 cells were performed at 30 °C with acquisition of 16 z-slices ...
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bioRxiv - Immunology 2022Quote: Splenic T-lymphocytes were counted and plated at an optimal seeding density of 8×104 cells per 100 µL in 96 well Microtitration ImageLock plates (VWR, #4379, Radnor, PA, USA). The cells were cultured with anti-CD3 and anti-CD28 activation beads and imaged over the course of 96hrs in the Essen Bioscience IncuCyte S3 Live-Cell imager and analysis system.
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bioRxiv - Microbiology 2022Quote: ... African green monkey kidney cells (Vero) were maintained in DMEM supplemented with 5% heat-inactivated fetal bovine essence (FBE) (VWR Life Sciences 10803-034), 1% Penicillin/Streptomycin (Gibco ...
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bioRxiv - Genomics 2023Quote: ... The resulting plasmid libraries were purified and concentrated via SPRI bead cleanup before being transformed into electrocompetent cells (Lucigen Endura, VWR International LLC #71003-038) for plasmid library amplification ...