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Citations for VWR :
951 - 1000 of 1155 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Cell pellets were then washed 1x with PBS and resuspended in RIPA lysis buffer (VWR, cat # 97063-270) supplemented with protease (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... purified cells were diluted and imaged at 100x magnification within a 20 μm counting chamber (VWR 15170-048). Images were analyzed using FIJI/ImageJ 1.52p (Java 1.8.0_172 ...
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bioRxiv - Immunology 2020Quote: ... 2.5 × 104 target cells were pipetted per well into 96-well black plates with clear-bottom (VWR/Corning) and kept in the dark for 20 min at room temperature ...
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bioRxiv - Genetics 2020Quote: ... after which the media was changed to complete growth medium and the cells expanded in T182.5 flasks (VWR) in monolayer and until confluent (between 10-14 days) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK T-REx cells were transfected with pcDNA4/TO/hTRPC3 using jetPRIME ® transfection reagent (VWR, Lutterworth, UK) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... For TPE-FLIM imaging the cells were seeded on 18 mm diameter microscope cover glass (VWR, Darmstadt, Germany) in PBS containing Ca2+ at room temperature.
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bioRxiv - Cancer Biology 2022Quote: ... the cells were then washed with PBS thrice and treated with 1.6 mM sodium metaperiodate (VWR, 13798-22) for 20 minutes at 4C for oxidation of the N-linked sugar residues ...
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bioRxiv - Cancer Biology 2022Quote: ... cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, VWR, PA, USA) assay ...
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bioRxiv - Cell Biology 2022Quote: Cells were fixed with methanol for 5 seconds and stained with Giemsa (1:10 dilution with MiliQ, VWR) for 17 min ...
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bioRxiv - Cell Biology 2022Quote: Caco-2 cells or human ileum organoids were lysed in 1 mL 20 mM Suprapur NaOH (VWR International) for 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated off and the cells were resuspended in 500 μl RiboZol RNA extraction reagent (VWR). Tubes were then vortexed and 100 μl chloroform was added to the tube ...
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bioRxiv - Plant Biology 2022Quote: ... The latter was captured in a 300 mL beaker and poured through a cell strainer (VWR, Radnor, USA) with a nominal pore size of 100 µm ...
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bioRxiv - Microbiology 2022Quote: ... cells from a rocking culture of swimmers were added to 60 mm non-treated tissue culture plates (VWR) in 2 mL of BHI ...
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bioRxiv - Biochemistry 2023Quote: Cells were seeded on a 24-well plate on 12 mm #1.0 glass coverslips (VWR, CAT# 631-1577P) in 500 µL media ...
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bioRxiv - Neuroscience 2023Quote: ... The solution containing dissociated neurons was passed through a 100 µm sterile cell strainer (VWR, Cat# 21008-950) into a 50 mL conical tube ...
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bioRxiv - Cell Biology 2023Quote: ... Swollen cells were then ruptured using pre-cooled dounce homogenizer and the tight pestle (VWR Cat. 62400-595). The ruptured cells were centrifuged at 218xg for 5 min to pellet nuclei ...
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bioRxiv - Immunology 2023Quote: ... at a density of 5 x 106 cells/mL in 100 μL of RPMI 1640 (#45000-396, VWR) supplemented with 10% fetal bovine serum (Omega Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Digested tissues were passed through 100 and 70 um nylon cell strainers (Corning, VWR# 10054-458, 89508-344). Single cell suspensions were washed by MACS buffer ...
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bioRxiv - Cell Biology 2023Quote: Frozen lentiviral stocks were generated by transfecting 70% confluent HEK-293T cells in 15cm cell culture dishes in ‘MEF medium’ together with a solution consisting of 1ml 150mM NaCl (1.06404.1000, VWR), 1ml Polyethylenimine (23966-1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were immediately flash frozen in liquid nitrogen in individual 0.2ml thin wall PCR tubes (731-0679, VWR) and stored at -80°C until further use (Supplementary Figure S1) ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: Cells and tissues were lysed by radioimmunoprecipitation assay (RIPA) buffer (0.2% IGEPAL CA-620, 0.02% SDS with VWR Life Science Protease Inhibitor Cocktail) ...
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bioRxiv - Microbiology 2023Quote: ... coli (E23) CRISPRi cell lines (details below) were grown overnight to stationary phase in LB Lenox Broth (VWR) before dilution 1:50 into fresh media in the morning ± 2 µM anhydrotetracycline ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed 3 x with Tris buffered saline (TBS) with 1% bovine serum albumin (BSA; VWR) and 0.5% Triton X-100 (Sigma ...
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bioRxiv - Physiology 2024Quote: ... cells were perfused with Ca2+-containing Krebs-Ringer’s solution supplemented with 0.1% fatty-acid free BSA (E588, VWR). To trigger ionic responses ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial cells were separated from the culture liquid by centrifugation and filtration through 0.22 µm membranes (VWR, USA). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were grown in 6-well plates after placing round coverslips in the bottom of the wells (VWR), or in PhenoPlates 96-well (Perkin Elmer) ...
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bioRxiv - Neuroscience 2024Quote: ... The solution containing dissociated neurons was passed through a 100 μm sterile cell strainer (VWR, Cat# 21008-950) into a 50 mL conical tube ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked for 1 hour in 2 ml of 3% BSA in PBS (VWR, cat#: 97063-624). Cells were then washed three times with 2 ml of PBS for 5 minutes each and mounted with Prolong Diamond Antifade Mountant (Molecular Probes ...
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bioRxiv - Biophysics 2024Quote: Experiments were done using a flow cell assembled by separating silanized hydrophobic coverslips and cleaned glass slides (VWR) by double sided tape (3M) ...
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bioRxiv - Bioengineering 2022Quote: ... the same batch of electrocompetent cells was also electroporated at various field strengths using 0.2-cm electroporation cuvettes (89047-208, VWR). One hundred microliters were pipetted into a pre-chilled electroporation cuvette ...
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bioRxiv - Immunology 2019Quote: ... cells were resuspended in 1 mL of cold sorting buffer (L15/10 mM HEPES/1 mg/mL BSA (VWR)/25 μg/mL DNase I (Roche)) ...
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bioRxiv - Cancer Biology 2019Quote: ... 100 µl of the cell suspension were injected using 25 G needles (BD Microlance TM 3. VWR 613-4952). Cell numbers and viability (required to be above 90% ...
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bioRxiv - Bioengineering 2019Quote: ... undifferentiated hPSCs were initially seeded at 1.0 million cells/1mL in ultra-low attachment 6 well plates (Corning, VWR) placed on a 9-Position stir plate (Chemglass ...
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bioRxiv - Immunology 2021Quote: Single-cell leukocyte suspensions were prepared from spleens by mechanical homogenization of tissues between frosted glass microscope slides (VWR) and filtration through a 70µm nylon mesh ...
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bioRxiv - Bioengineering 2020Quote: RAW 264.7 macrophages were seeded at 50,000 cells per well in a 96 well black glass-bottom plate (Greiner 96 well plates, 655891, VWR). Cells had been previously incubated with folic acid deficient RPMI medium (10% FBS ...
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bioRxiv - Bioengineering 2020Quote: H1299 cells were seeded at 20,000 cells per well in a 96 well black glass-bottom plate (Greiner 96 well plates, 655891, VWR). Media was replaced with media containing treatments (1% DMSO ...
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bioRxiv - Cancer Biology 2020Quote: ... MSCs were plated at 1.75×105 cells per 4.2cm2 of a 6-well transwell insert (Falcon 353090, VWR, Germany).
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed once in water followed by mounting the coverslip onto glass slides with Vectashield (VWR, 101098-042) and finally sealing the coverslip with nail polish (Electron Microscopy Science Nm ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then cell suspensions were added with 20% (w/v) glass beads (0.5 mm) and the cells were grinded with hand-powered electrical motor (VWR). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... 10 μl of the cell lysates were resolved by standard 10% SDS-PAGE gel and electroblotted onto 0.2 mm nitrocellulose membrane (Biotrace, VWR) using a semi-dry transfer unit (Bio-Rad) ...
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bioRxiv - Biophysics 2021Quote: Cells were seeded at low density (~ 10 000 cells/ml) on 24 × 50 mm coverslips with thickness of #1.5 (VWR). After a settling time to allow cells to adhere ...
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bioRxiv - Cell Biology 2021Quote: ... Japanese Collection of Research Bioresources Cell Bank) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, VWR) and an antibiotic mixture (penicillin/streptomycin (PenStrep) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed by placing the plates on a shaker after adding 250 μL 0.1 M NaOH (VWR, US). Aliquots of 150 μL were pipetted out of each well into a scintillation bottle (Perkin Elmer ...
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bioRxiv - Neuroscience 2022Quote: ... the cell strainer was removed from the testing arena and dropped into a 6-well dish (VWR 10861-554) containing 4% PFA in 1x PBT (1x PBS + 0.25% TritonX100) ...
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bioRxiv - Microbiology 2020Quote: ... and MD40HG cultures in 0.2% FLA-YM (as above) and a no cell negative control were filtered through 0.2 μm cellulose acetate membrane syringe filter (VWR). The filtrates were flash frozen and stored at −80°C until analysis ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 1% penicillin-streptomycin and 1% FBS at a density of 250,000 cells per well in 24 well plates (Nunclon) on coverslips precoated with poly-d-lysine and Matrigel (VWR) diluted 1:200 in Minimum Essential Media (HyClone) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were serum-starved 16 hours prior to plating with 1% bovine serum albumin (VWR, Cat. no. 0332-100G) added for protein supplementation ...
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bioRxiv - Microbiology 2022Quote: ... Each transformation consisted of 40μL of competent cells and 10ng of the pFAB5516 plasmid library transferred to a chilled cuvette (1mm gap, VWR). Cuvettes were electroporated using a BTX™-Harvard Apparatus ECM™ 630 Exponential Decay Wave Electroporator with the following parameters ...
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bioRxiv - Genomics 2019Quote: Cells were harvested and total genomic DNA was isolated using QuickExtract DNA Extraction Solution (VWR, Radnor, PA, cat# QE09050). PCR was prepared using 5X GoTaq Green Reaction Buffer and GoTaq DNA Polymerase (Promega ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were homogenized in approximately 200 μL of PBS using pre-filled silica bead tubes (VWR, Radnor, Pennsylvania) and a Bead Ruptor Elite homogenizer (OMNI International ...