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Citations for VWR :
1001 - 1050 of 1155 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... cells were seeded at a density of 1.0×106 cells on day 0 in fifteen tissue culture dishes (10 cm diameter, ref 25382-166, VWR) in DMEM High Glucose ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were further dissociated with 3ml TrypLE Express, washed with Wash media (DMEM, 0.1% BSA) + 1mg/ml DNase (VWR) and centrifuged at 300 rcf for 3 mins ...
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bioRxiv - Cancer Biology 2020Quote: ... through injection of 5×105 67NR murine luminal breast cancer cells in 100 μL of serum-free RPMI (VWR). 67NR cells are locally invasive but non-metastatic ...
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bioRxiv - Cell Biology 2020Quote: Cells were washed twice with ice-cold PBS containing 50 μg/mL of cycloheximide (CHX) (VWR, 94271, Radnor, PA) for 3 minutes ...
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bioRxiv - Immunology 2021Quote: ... washed with AIMV medium without FCS and settled at a number of 2.5×104 cells per well into a 96-well black plate with clear bottom (#734-2480, VWR) for around 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... 2.5 × 104 target cells per well were pipetted into a 96-well black plate with clear-bottom (#7342480, VWR). After a short rest (15 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were transfected with hTRPC6 (cloned into pcDNA3) or rat TRPV1 using jetPRIME ® transfection reagent (VWR, Lutterworth, UK). Cells were assayed 48 hours after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... HT-1080 cells were purchased from ATCC and cultured with DMEM supplemented with 10% fetal bovine serum and 0.1% Gentamycin (VWR). SUM149 (SUM149PT ...
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 5% (vol/vol) fetal bovine serum (FBS, VWR) and 1% penicillin–streptomycin–glutamine (PSG ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 million cells were seeded on a 15cm plate containing one 25 mm plastic coverslip (Thermanox, VWR 100500-878). At 24 hours after seeding ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed once with warm PBS and changed to a medium of DMEM without glucose (VWR, 97060-876), 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Ramos cells were washed twice and 8.0 x 105 were aliquoted into thirteen 12×75 mm glass reagent tubes (VWR) for each test antibody ...
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bioRxiv - Immunology 2023Quote: ... Cell pellets were diluted in 1 mL PBS and they or BALF were shaken in a glass vial (VWR) with 2 mL chloroform and 1 mL methanol containing internal standards (13C16-palmitic acid ...
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bioRxiv - Bioengineering 2023Quote: The cell culture plate inserts (transwells) for 24 wells (PET membrane, 3.0 µm pore size) were purchased from VWR International.
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bioRxiv - Immunology 2023Quote: ... a total of 107 cells were resuspended in 80μl PBS buffer supplemented with 0.5% BSA (0332, VWR Life Science) and 2mM EDTA (E57040 ...
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bioRxiv - Developmental Biology 2023Quote: ... we used a brush to release the eggs from the agar and transferred them to a cell strainer with 100µm nylon mesh (VWR). To remove the chorion ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were typically grown in 6-well plates after placing round coverslips in the bottom of the wells (VWR), or in PhenoPlates 96-well (Perkin Elmer) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed in PBS and fixed/stained in crystal violet/methanol solution (25% (v/v) methanol (VWR #20847.307) + 5% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...
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bioRxiv - Developmental Biology 2024Quote: ... which were adjusted to a concentration of 1.25 × 106 cells/mL in iPS Brew + 10 µM ROCK inhibitor (Y-27632, VWR). 800 µL of cell suspension (approximately 1 million cells ...
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bioRxiv - Evolutionary Biology 2024Quote: ... HEK293T cells were seeded at a density of 0.6 x 106 cells on day 0 in 60 mm culture dishes (ref 25382-100, VWR) in DMEM High Glucose ...
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bioRxiv - Biophysics 2020Quote: ... The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Briefly, HEK293T cells (ATCC catalog #CRL-3216, RRID:CVCL_0063) were grown in DMEM supplemented with 10% FBS (VWR, catalog #97068-085) and 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... 10 μL of the labeled cell suspension was gently pipetted onto 22 x 22 mm No.1 cover glass (VWR) and sealed onto glass slides with clear acrylic nail polish for confocal imaging or onto Lab-Tek chambered #1 cover glass (Thermo Fischer Scientific)(for widefield fluorescence) ...
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bioRxiv - Genomics 2020Quote: ... Cells expressing Cas9 were selected either by sorting for GFP10 or selection with 350 ng/ml Puromycin dihydrochloride (VWR J593) for three consecutive days starting one day after electroporation17 ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C on a rotator ...
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bioRxiv - Immunology 2019Quote: ... cells were plated at a density of 1 x 105 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Neuroscience 2020Quote: ... penicillin/streptomycin and amphotericin B (1X Anti/Anti, #15240062, TFS) (referred to as ‘SHM-10’) on uncoated cell culture flasks (VWR). Cultures were maintained in a sterile incubator (MCO-20AIC ...
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bioRxiv - Cell Biology 2021Quote: ... then lysed cells in 1mL of ice-cold nuclear isolation buffer in a 2mL Dounce homogenizer (VWR, cat. #62400-595). We next filtered the homogenate through a 40µm Flowmi cell strainer (WVR ...
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bioRxiv - Biochemistry 2021Quote: For transductions VeroE6 or HEK cells were seeded into greiner CELLSTAR® 96-well plates or 6-well plates (VWR) containing lentivirus in DMEM containing 2 % FBS and 1 μg/mL polybrene ...
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bioRxiv - Microbiology 2020Quote: ... Cell debris and unbroken cells were removed by centrifugation and supernatants were passed through a 0.22 μm filter unit (VWR International). Soluble protein suspension was loaded onto the Bio-Scale Mini Profinity IMAC Cartridge (5 mL ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... 20 μl of cells were put to 180 μl of TYI-S-33 media in 96-wells Tissue Culture Plate (VWR) and the same dilution step was performed 5 times ...
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bioRxiv - Immunology 2022Quote: ... We washed the plate with PBST (Applied Cells, Inc.) five times and added 100 μl 5% BSA (VWR Life Sciences) into each well and incubated at 37°C for 90 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Cells on D-poly-Lysine coated coverslips were then fixed with pre-warmed 3.7% formaldehyde (v/v; VWR, VW3408-1), permeabilized with ice-cold methanol ...
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bioRxiv - Neuroscience 2020Quote: ... Human neuronal cells SH-SY5Y (ATCC, Manassas, VA, #CRL-2266) were cultured in DMEM medium (VWR, Radnor, PA, #0101-0500) supplemented with 10% FBS (Gemini Biosciences West Sacramento ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... PC3-GFP KPC and NIH-3T3 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, cat. no. 16777-129, VWR international, LLC.) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then subcultured 1:1000 into 7 mL of MSgg in a 6-well polyvinyl plate (VWR, PA, USA). Plates were incubated in static conditions at 30°C for 3 days and were then imaged using a Leica MZ10F macroscope and Leica DMC2900 camera ...
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bioRxiv - Neuroscience 2022Quote: ... clear-bottomed, 96-well plates (Falcon, Switzerland) at a density of 200,000 cells/mL or onto coverslips (CS) (VWR, Switzerland) at a density of 250,000 cells/mL ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) and were mounted onto Superfrost Plus microscope slides (VWR) in VECTASHIELD antifade mounting medium (Vector Laboratories) ...
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bioRxiv - Genetics 2019Quote: ... Four million S2 cells that were stably transduced with RasV12Common and RasV12Rare plasmids were seeded into coated tissue culture dishes (60×15mm, VWR) with 2 ml of Schneider’s media (without FBS) ...
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bioRxiv - Physiology 2020Quote: ... containing 10% serum and centrifuged at 50 g for 5 min to enrich hepatocytes and passed through a 100-micron nylon cell strainer (10199-659, VWR) multiple times ...
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bioRxiv - Cell Biology 2020Quote: ... we fixed 250 μL of cells in 1000 μL of ice-cold methanol in a 2 mL plastic tube (16466-060, VWR). For wounded cells ...
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bioRxiv - Immunology 2020Quote: Cells of human or murine origin (0.2-1×106) were lysed in Trizol (peqGOLD TriFast™; VWR International, Radnor, USA) and total RNA was extracted by use of the DirectZol® RNA MiniPrep (Zymo Research ...
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bioRxiv - Cell Biology 2021Quote: ... Cell suspension was filtered through 35 μm nylon filter cap on 12x75 mm round bottom tubes (VWR/Falcon 21008- 948). Flow cytometric measurements were performed using a BD LSR Fortessa ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were seeded on 24-well plate (0.5 x 105 cell/well) in growth medium (DMEM medium; Thermo Fisher Scientific, 10569010, fetal bovine serum; VWR, 97068-085 ...
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Single-cell assessment of trophoblast stem cell-based organoids as human placenta-modeling platformsbioRxiv - Developmental Biology 2022Quote: ... The supernatant from the second and third digestions were then pooled and strained through a 100 μm cell strainer (VWR). Pooled and digested villous suspensions were then centrifuged at 4°C at a speed of 1200 rpm for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: B16-F1 cells were transfected with 0.5 µg EGFP-β-actin and 1 µL JetPrime transfection reagent (VWR, #114-07) per well in 6-well plates according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Cryopreserved PBMCs were thawed and washed in RPMI before resuspension in buffer (0.2% FBS in PBS) at concentration of 1 × 106 cells/ml in FACS tubes (Falcon® Brand, VWR). Before addition of the cocktail of 21 monoclonal antibodies ...
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bioRxiv - Neuroscience 2022Quote: The coverslip seeded with the transfected cells was placed in a custom glass-bottom chamber based on Chamlide EC (Live Cell Instrument) with glass bottom made with a 24 × 24 mm #1 coverslip (89082-270, VWR). Cells were continuously perfused with the external solution at ∼ 4 mL/min with a peristaltic pump (505DU ...