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Citations for VWR :
1001 - 1050 of 1068 citations for 3 4 Fluorophenyl 5 fluorobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The 5 L bioreactors were grown for one to two weeks pl shredded (VWR 200 Homogenizer on high for one minute) and transferred to 12L bioreactors ...
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bioRxiv - Immunology 2020Quote: ... diluted to 5 µg/ml in PBS containing 2% (w:v) Fraction V BSA bovine serum albumin (PBS-BSA) (VWR, Radnor, PA). Slides were then washed in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... one holes were bored into the lid of a Drosophila bottle and inserted a 5-μl glass capillaries (VWR, 53432-604) which filled with 20% sucrose ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and C were filled with 25 µl of 40 µM oligomycin for a final in-well concentration of 5 µM (“Oligo,” VWR Scientific), 4.5 µM carbonyl cyanide-p-trifluoromethoxyphenylhydrazone for a final in-well concentration 0.5µM (“FCCP,” VWR Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... we added another 0.5 ml PBS to the tubes that later were sonicated in the water bath of an ultrasonic cleaner (USC200TH, VWR International) for 5 min at 45 kHz ...
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bioRxiv - Cancer Biology 2024Quote: A total of 5 × 106 MM.1Sgfp+/luc+ cells were seeded in T-25 flasks (Avantor/VWR, Cat. No. 10861-568) and treated with the vehicle (DMSO ...
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bioRxiv - Developmental Biology 2023Quote: ... The 18 cryovials were tightly capped and placed in a 5 cm square cardboard cryobox that was immediately placed in a LN dewar (VWR CryoPro).
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bioRxiv - Immunology 2023Quote: ... brains were isolated and placed into a 50 mL conical tube (Fisher Healthcare) containing 5 mL of RPMI (VWR International LLC). The RPMI containing the brains of the mice were then transferred to a 7 mL glass Tenbroeck dounce homogenizer (Pyrex ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred to a PVDF membrane for 1.5 H at 400 mA and blocked using 5 % BSA (VWR, 103219-864) rocking at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a ratio of 1:5 (chloroform:trizol) for aqueous separation of RNA using heavy gel phase-lock tubes (VWR, 10847-802). Library preparation and RNA-sequencing was performed by Genewiz (Azenta Life Sciences) ...
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bioRxiv - Genetics 2023Quote: ... Starved flies were transferred to vials containing 600μl of Paraquat solution or 5% sucrose (mock treatment) as well as a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed three times with 1X PBS (5 minutes each) and mounted on SuperFrost Plus micro slides (VWR 48311-703). Slices were covered with a 22 mm x 50 mm 1.5 thickness coverslip using ProLong Glass Antifade Mountant media (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... A 5 mg aliquot of each sample was subjected to extraction using methanol (HPLC - gradient grade, VWR International LLC, Randor, USA). The extraction process involved homogenization with 10 ceramic balls at a velocity of 6.5 m/s for 30 seconds ...
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bioRxiv - Physiology 2024Quote: ... permeabilized with 3% Triton X 100 solution for 15min (Carl Roth, Germany) and blocked with 5% Goat Serum (BioWest, France) and 1% Bovine serum (VWR, USA) for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... which consists of three steps: 1) Cells were seeded into uncoated T75 flasks (4×106 viable cells/flask, VWR, Cat# 734-2788, Radnor, PA) for 18 hrs to remove fibroblasts ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were extracted and fixed for a minimum of 24 hours in PFA at 4 °C and transferred to a 2 % dimethyl sulfoxide solution (VWR International, Radnor, PA, USA) prepared in PB for at least 24 hours at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... One-day-old biofilms were grown on the surface of a 4-well Permanex slide (Lab-Tek® Chamber Slide™ System, VWR, Debrecen, Hungary). The preformed biofilms were washed three times with sterile physiological saline ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Viable embryos at the 2-cell stage (0.75 hours post fertilization (hpf)) were sorted into 4 replicates of 10 embryos within 24-well plates (VWR international, LLC Radnor, PA, USA). Replicates were exposed to 1 mL of vehicle (0.1% DMSO ...
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bioRxiv - Microbiology 2021Quote: ... The entire amount of plasma (about 180 µL) was filtered using 0.2 µm centrifugal tube from VWR (5 min, 10°C, 10000xg). Next ...
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bioRxiv - Cell Biology 2020Quote: Gastrocnemii were homogenized with 25 strokes using a 5 mL PTFE tissue grinder with clearance 0.15-0.25 mm (VWR 89026-392, 89026-404) at 3,000 rpm in RIPA lysis buffer (VWR 97063-270 ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 50 ng/ml recombinant murine macrophage-colony stimulating factor (rmM-CSF, Genentech)] and plated in 15-cm non-TC treated dishes for 5 days (Petri dish, VWR, 25384-326). Fresh BMDM media was added on day 3 without removal of original media ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.4 μl of each primer and 5 μL VWR Red Taq DNA polymerase Master mix (VWR Chemicals, Denmark, Cas nr: 733-2547). The PCR reaction conditions for amplification of DNA were as follows ...
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bioRxiv - Physiology 2021Quote: ... incubated at 37°C for 120 min and inactivated at 85°C for 5 min in a thermal cycler (VWR UNO 96). cDNA was then stored at −20°C until further use ...
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bioRxiv - Microbiology 2021Quote: ... Gryphon cells were cultivated according to manufacturer’s instructions at 5% CO2 37 °C in 10 cm tissue culture-treated dishes (VWR Canada, Mississauga, ON) in DMEM-high glucose (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... The 40 μL solution of the lipid mixture was spread on a 5% w/v PVA film dried on a 25 x 25 mm coverslip (VWR, Radnor, PA) and then put under vacuum for at least 2 h to form a dry lipid film ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was changed and the incubation continued for another 24 hours before addition of 1-5 µg/mL puromycin (VWR 97064-280). Incubation with puromycin continued for several days until untransfected control wells were depleted of cells ...
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bioRxiv - Biophysics 2021Quote: ... and subsequently incubated at 37 °C and 5% CO2 for 2 h before seeded and imaged on an 18 mm diameter microscope cover glass (VWR, Darmstadt, Germany) in PBS containing Ca2+ (Gibco ...
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bioRxiv - Genomics 2021Quote: ... and myotubes from one 10 cm plate (∼5 million cells) with >90% viability were lifted as described above and pelleted in 15 mL polypropylene falcon tubes (VWR, 89039-670) by centrifuging for 5 minutes at 1500 RPM ...
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bioRxiv - Cell Biology 2021Quote: Aggregation was induced by agitating 5 mg/mL α-synuclein at 37 °C for 7 days 1200 rpm (VWR, Cat#12620-942). Fibril formation was evaluated by electron microscopy (see next section for details) ...
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bioRxiv - Physiology 2020Quote: ... 5-μm sections were stained with hematoxylin and eosin (H&E) using the Leica Autostainer and mounted in DePeX (VWR, Lutterworth, UK). Adipocyte diameter and pancreatic β-cell islet number and size were quantified using ImageJ software as previously described (7 ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA/DNA hybrid was tagmented in TD reaction buffer (10 mM Tris-Cl pH 7.6, 5 mM MgCl2, 10% DMF) supplemented with 3.4% PEG8000 (VWR Life Science, Cat.No.97061), 1 mM ATP (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... decalcification solution for 14 days and then paraffin embedded for sectioning with a Leica RM2235 microtome at 5 μm per section to generate histology slides (VWR 631-1553). Sections were processed and stained with TRAP solution and counterstained with 0.2% methyl green solution (Bioenno Lifesciences ...
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bioRxiv - Neuroscience 2023Quote: ... The PVDF membranes were blocked in 5% Nonfat dry milk (Bio- Rad Laboratories, Cat.# 1706404) in Tris-buffered saline with 0.1% Tween 20 (TBST) (VWR, Cat.# K873-4L). Samples were probed with the primary antibodies listed in Supplemental Table 1 overnight in 5% bovine serum albumin (Gold Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were put into 5’polystyrene chromatography columns with coarse filters (Evergreen, 208-3383-060) containing DEAE Sephadex A-25 resin (VWR, 95055-928). The columns were equilibrated in high salt buffer (20mM Tris-HCL ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then loaded into 5’polystyrene chromatography columns with coarse filters (Evergreen, 208-3383-060) containing DEAE Sephadex A-25 resin (VWR, 95055-928). Once the sample completely flowed through the column ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown at 21°C in shaking culture in HL5 medium (Formedium, Norfolk, England) and on SM/5 agar plates (2 g/L glucose (VWR, Solon, OH), 2 g/L bacto peptone (Becton ...
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bioRxiv - Biophysics 2023Quote: ... The 40 μL solution of the lipid mixture was spread on a 5% w/v PVA film dried on a 25 x 25 mm coverslip (VWR, Radnor, PA) and then put under vacuum for at least 2 h to form a dry lipid film ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Equal volumes (5 μL) of the resulting PCR product were loaded onto a 2% biotechnology grade Agarose I (VWR International, PA, USA) gel prepared with 1X TBE buffer and 0.0025% ethidium bromide ...
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bioRxiv - Biochemistry 2022Quote: The eluates of SILAC “heavy” and “light” were combined for each SA+ and SA-group respectively followed by reduction in 5 mM TCEP (VWR, #97064-848) for 1 hour and alkylation in 10 mM iodoacetamide (VWR ...
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bioRxiv - Biophysics 2022Quote: ... peptides were resuspended in 20 μL of resuspension buffer (5:95 ACN:MilliQ H2O, 0.1% TFA) and solubilized in a bath sonicator (VWR, 35 kHz, 10 min). A ZipTip was placed on a P10 pipettor set to 10 μL ...
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bioRxiv - Microbiology 2022Quote: ... 1 cell line was cultured at 37°C with 5% CO2 in DMEM with high glucose and L-glutamine (VWR 16777-129) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-2 preps of in vitro transcribed RNA and 5-10µg N gene RNA were mixed with the prepared cell suspension in a 4mm electroporation cuvette (VWR International # 89047-210) and pulsed in a BioRad GenePusler using the exponential protocol with the following settings ...
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bioRxiv - Microbiology 2022Quote: ... containing 1.5 ml of brain–heart infusion broth ([BHI]; Becton Dickinson, Franklin Lakes, NJ) supplemented with 0.1% Tween 80 (VWR Chemicals, Solon, OH) and were incubated at 37°C with 5% CO2 ...
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bioRxiv - Genetics 2023Quote: ... 500μl of concentrated bacterial suspension or 5% (w/v) sucrose solution (mock treatment) was added to empty fly vials containing a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Immunology 2024Quote: Single cells prepared from tumor tissues were counted and 2-5 million cells were plated on a 96-well plate (VWR, round botom) and washed with PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl, pH 7.2, 5 mM EGTA, 100 mM KCl, 1% Triton-X100; all chemicals from VWR; Radnor, PA, USA). Samples were homogenized using tight-fitting pestles and centrifuged at 3,000 g for 30 minutes at 4ºC ...
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bioRxiv - Bioengineering 2024Quote: ... The cell suspension was then centrifuged at 300g for 5 mins and re-suspended in DMEM/F-12 with L-glutamine (VWR, Radnor, PA), 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were then centrifuged at 300 x Gs for 5 min and the pellet was resuspended in DMEM/F-12 with L-glutamine (VWR, Radnor, PA), 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2024Quote: ... SIH5841.5), 1960 µL of deionized (DI) H2O (PURELAB Flex, ELGA LabWater) and 120 µL of 0.5 M HCl (VWR, catalog no. 20246.298). The solution was stirred vigorously for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were mounted on a thin (∼1-2x lab tape thickness) 2% agar pad on a glass slide (VWR VistaVision, 3 inches x 1 inch x 1 mm) using a hand pulled borosilicate glass capillary pipette (World Precision Instruments ...