Labshake search
Citations for VWR :
851 - 900 of 1068 citations for 3 4 Fluorophenyl 5 fluorobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... The cortex was dissected and homogenized into a single cell suspension using a tissue homogenizer (5 cm3, VWR) and filtered m strainer (Falcon) ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were again washed in PBS for 6 × 5 mins before mounting onto polylysine-coated slides (Polysine, VWR). Mounted slices were left to dehydrate overnight before applying coverslips using either Aqua-Poly/Mount (Polysciences Inc. ...
-
bioRxiv - Neuroscience 2021Quote: ... In short, free-floating sections were blocked (5% Goat Serum (BioWest, France) and 1% Bovine serum (VWR, USA) and then incubated with different primary antibodies ...
-
bioRxiv - Zoology 2021Quote: ... the samples were passed through a 5–15 μm pore size paper membrane (VWR, Cat No. 516-0813) by gravity filtration to avoid damaging the cells– M ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... A 5 mL syringe was connected to a swivel using Tygon* tubing (AAQ04103; VWR; West Chester, PA, USA). Each swivel was coupled with a spring leash (C313C ...
-
bioRxiv - Microbiology 2023Quote: ... Mat samples (approximately 5 g) were homogenized in 15 mL disposable tissue grinders (VWR International, Radnor, PA, USA). Once ground ...
-
bioRxiv - Bioengineering 2022Quote: ... Devices were washed three more times with PBS and blocked in PBS 5% bovine serum albumin (BSA, VWR) overnight at 4 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cell pellet was lysed with 500 μL of Cell Lysis Buffer [5 mM PIPES (VWR, 0169-100g), 85 mM KCl ...
-
bioRxiv - Microbiology 2022Quote: ... gondii co-cultures were maintained at 37°C with 5% CO2 in D10 medium (Dulbecco’s Modified Eagle Medium, 4.5 g/L glucose, VWR) supplemented with 10% Cosmic Calf serum (HycloneTM ...
-
bioRxiv - Cell Biology 2022Quote: Cells were fixed with methanol for 5 seconds and stained with Giemsa (1:10 dilution with MiliQ, VWR) for 17 min ...
-
bioRxiv - Genomics 2022Quote: RNAscope assay was performed on lung 5 μm FFPE sections on Superfrost Plus microscope slides (#631-9483, VWR) cut from depths consecutive to the sections mounted on Visium slides ...
-
bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated in blocking solution [5% milk in PBST (PBS (pH 7.4) with 0.1% Tween-20 (VWR))] for 2 h and probed overnight with the following primary antibodies ...
-
bioRxiv - Cancer Biology 2024Quote: ... permeabilized in 0.5% Triton X-100 (MilliporeSigma) for 10 min and blocked with 5% bovine serum albumin (VWR) for 30 min ...
-
bioRxiv - Microbiology 2024Quote: ... Supernatant was diluted 1:5 in PBS and added to 384 well ELISA plates (VWR, cat. CA62409-064) that had been previously coated with 1 𝜇g/mL NeutrAvidin (Thermo Fisher ...
-
bioRxiv - Cell Biology 2020Quote: ... After 1h binding on a wheel at 4°C beads were washed 3x in PBS supplemented with 0.02% Tween20 (VWR, M147) and 1% SDS and eluted with NuPAGE LDS buffer supplemented with 50 mM DTT and 1 mM biotin at 95 °C for 10 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were centrifuged at 14000 rpm at 4°C and the supernatants clarified with a 0.2 μm membrane syringe filter (VWR International) before proceeding with a two-step purification protocol ...
-
bioRxiv - Systems Biology 2020Quote: ... 4°C) and the soluble fraction was collected and protein precipitated using ice-cold acetone (25% final concentration, VWR, USA) for 1h at - 20°C followed by centrifugation (400x g ...
-
bioRxiv - Microbiology 2020Quote: ... and 1 volume of phenol (pH 4.3):chloroform (1:1), followed by centrifugation (16000 x g, 4 °C, 10 min) in a phase lock tube (Quantbio, VWR) to separate the aqueous and organic phases ...
-
bioRxiv - Genomics 2021Quote: Tissue sections were prepared from formalin-fixed paraffin embedded tissue blocks and cut to 4 micrometers serial sections and mounted on Superfrost Plus (VWR). The procedure for multiplex immunohistochemistry (mIHC ...
-
bioRxiv - Physiology 2021Quote: ... on a shaker at 4°C in tris-buffered saline with 1% tween 20 (TBS-T; VWR Amresco ProPure M147) and 0.1 g ml-1 skim milk powder (Kroger ...
-
bioRxiv - Plant Biology 2021Quote: ... one of tissue piece (50 mg) was ground using a 4-mm steel ball in a 2-ml Eppendorf tube in a bead beater (VWR) for 1 min at 30 revolutions·s−1 ...
-
bioRxiv - Biophysics 2020Quote: ... The cell lysate was separated from insoluble material by centrifugation at 40,000 g for 60 minutes at 4 °C and subsequently incubated with a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C on a rotator for affinity chromatography via the 6xHis-tag ...
-
bioRxiv - Plant Biology 2022Quote: ... 0.4 g of poly(FATRIFE-ter-PEGMA-ter-NBMA) terpolymer was dissolved in 25ml methyl-ethyl ketone (25642.325, VWR, UK) in a 500 ml rotary evaporator flask containing 20 g of FEP at 40 °C to coat FEP particles ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated at 4℃ to maintain their viability and passed through a 40 µm cell strainer (VWR, 76327-098) to dissociate single cells from clumps ...
-
The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
-
bioRxiv - Cell Biology 2021Quote: ... After solidification of the gelatin block for 6h at 4°C it was embedded in a larger OCT block (Qpath, VWR) and subjected to cryosectioning (10 µm ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
-
bioRxiv - Cell Biology 2020Quote: Melanocytes were cultured in 10-cm dishes and fixed in situ with Karnovsky’s fixative [4% paraformaldehyde (VWR Scientific, Radnor, PA), 4 mM calcium chloride ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... Blood plasma was obtained by centrifuging at 5000 rpm for 10 minutes at 4 °C and stored in cryotube vials (VWR Micro-centrifuge tubes with graduation 2.0 mL ...
-
bioRxiv - Molecular Biology 2023Quote: Recombinant Jag1-Fc was immobilized by overnight incubation at 4°C in individual wells of non-tissue culture-treated 6 or 12 well plates (VWR) at a final concentration of 2 µg/mL in DPBS containing 10 µg/mL poly-D-lysine (ThermoFisher Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: ... washed once in ice-cold 1× PBS with Ca2+/Mg2+ and then fixed in 4% paraformaldehyde (PFA, VWR: 100504-940) for 30 mins at room temperature ...
-
bioRxiv - Biophysics 2023Quote: HeLa cells were fixed using 4% paraformaldehyde and permeabilized using Triton-X100 (VWR, Radnor, PA, USA, Cat. No. M143-1L) 12 hours after TTLL6-YFP transfection ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were fixed by adding 100 µl 8% PFA for 20 min at RT (final concentration of PFA is 4%; PFA from VWR). All media was then gently removed and cells were washed 3 times for 5 minutes with 150 µl PBS ...
-
bioRxiv - Physiology 2024Quote: ... were then exposed to cold treatments of -15°C for up to 48 hours (in increments of 4, 8, 12, or 48 hours) in a cooling circulator (VWR Scientific 1167P Heating/Cooling Recirculating Water Bath ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris precast gels (Fisher Scientfic, WG1402BOX) and then transferred to 0.45μM nitrocellulose membranes (VWR, 10120-006). Membranes were then blocked with 5% nonfat dairy milk in Tris-buffered saline containing 0.1% Tween 20 (VWR ...
-
bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
-
bioRxiv - Microbiology 2024Quote: ... then pelleted at 3200 x g for 10 minutes at 4°C and washed twice with pre-chilled blood bank saline (89370-096, VWR). Pellets were resuspended in 0.8 mL 3:1:0.004 acetonitrile:methanol:formic acid + 10 nM verapamil (V105 ...
-
bioRxiv - Genomics 2024Quote: ... The suspension containing heads was poured through a 100 µm cell strainer to separate tissue from the collection sucrose buffer and separated into 4 equally aliquots that were each separately homogenized with 7 gentle strokes in a KONTES Tissue Grinder (VWR) (loose pestle ...
-
bioRxiv - Developmental Biology 2024Quote: ... 20 to 30 animals per treatment were incubated at 4°C for 1 hour and then transferred into an electroporation cuvette (Gene Pulser/Micro Pulser Electroporation Cuvette 0.4-cm gap, Bio-Rad or electroporation cuvettes 4-mm gap, VWR) and washed twice with chilled Milli-Q water ...
-
bioRxiv - Developmental Biology 2024Quote: Embryonic tissue samples from different embryonic days were fixed overnight at room temperature in 4% paraformaldehyde solution (VWR, Pennsylvania, USA), followed by dehydration and embedding in paraffin ...
-
bioRxiv - Molecular Biology 2023Quote: ... we seeded 2×104 ESCs per well of a Geltrex-coated 12-well plate for subsequent RT-PCR and RT-qPCR analyses and 1-3×103 cells per well of a 12-well plate containing a Geltrex-coated 18-mm glass coverslip (VWR, cat# 631-1580) for immunostaining ...
-
bioRxiv - Cancer Biology 2024Quote: ... Supernatant was removed and replaced with 500 μL of ACK (ammonium-chloride-potassium) lysis buffer per sample to remove red blood cell contamination (3 min incubation at 37°C; VWR cat#10128-808). Reaction was quenched with 10 mL of cold DMEM/F12-Advanced media supplemented with 10% FBS,1% L-glutamine ...
-
bioRxiv - Microbiology 2021Quote: ... ‘Fast’ shaking was established by orbital rotation of flasks at setting 4 on an orbital shaker (Model 3500, VWR, Radnor, PA), which corresponds to ∼200 rpm ...
-
bioRxiv - Cancer Biology 2021Quote: ... Matrigel containing organoid samples were removed from multi-well tissue culture dishes (using a 3.5 mL disposable transfer pipette and placed in 45 mL of cold PBS (4°C) in 50 mL capacity polypropylene centrifuge tubes (VWR Scientific). Matrigel was washed away by 3-4 rounds of suspension in cold PBS and centrifugation using a refrigerated centrifuge set to 1,000 pm ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted at 5,000 rpm for 15 minutes at 4°C and resuspended in 10 mL of lysis buffer [300 mM NaCl (VWR BDH9286), 20mM Tris pH 7.5 ...
-
bioRxiv - Neuroscience 2021Quote: ... mice were deeply anaesthetized with isoflurane (4% flow rate of 0.5–0.7 l/min) and transcardially perfused with cold phosphate-buffered saline (PBS) followed by 4% PFA (VWR International Inc, 15710). Brains were extracted and placed in PFA for 24 h and then transferred to 30% sucrose/PBS solution at 4 °C ...
-
bioRxiv - Physiology 2023Quote: ... the sulfhydryl groups were derivatized by transferring the final supernatant to 1.55 M NaOH and Na2B4O7·10H2O (125 mM with 4 mM EDTA, pH 9.5, VWR, Radnor, PA, USA) solutions ...
-
bioRxiv - Bioengineering 2023Quote: ... conditioned media was centrifuged at 3000 RPM for 10 minutes at 4°C and the supernatant was filtered using 0.2 μm syringe filter (VWR, 28143-310). Sample placed in ultracentrifuge tubes (Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... After overnight plating cells were treated with different doses of CDDP for 24 hours or with MMS for 8 hours and fixed with 4% PFA (VWR, AA47377) for 20 minutes and stored in PBS at 4 °C ...
-
bioRxiv - Immunology 2024Quote: ... tubes with mAb were incubated on a rotator at 4 °C overnight on a rotator (Model 13916- 822, VWR, United States). These mAb included anti-histone H3 (1:50 ...