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Citations for VWR :
901 - 950 of 1068 citations for 3 4 Fluorophenyl 5 fluorobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... the sulfhydryl groups were derivatized by transferring the final supernatant to 1.55 M NaOH and Na2B4O7·10H2O (125 mM with 4 mM EDTA, pH 9.5, VWR, Radnor, PA, USA) solutions ...
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bioRxiv - Immunology 2024Quote: ... Plaque assays were developed at 4 days post infection by removal of overlay medium and staining/fixation using 10% neutral buffered formalin (VWR, #89370) and 0.25% crystal violet (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... overnight at 4°C followed by a goat anti-rabbit IgG secondary antibody conjugated to HRP (VWR, cat. no. 102645-182). F1β signal was then captured using the same method.
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bioRxiv - Biophysics 2024Quote: ... at 4°C overnight in a 35 mm Mat-Tek glass coverslip bottom dish (VWR; Catalog No. P35G-0-14-C). After the poly-D lysine coating ...
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bioRxiv - Cell Biology 2024Quote: ... cells were harvested and sonicated with a Branson SFX250 Sonifier with 102-C Converter and a 3/4" High-Gain Horn in cold lysis buffer containing 50 mM sodium phosphate-NaOH pH 7.5 (Oakwood #099682, VWR #97064-480), 150 mM NaCl (Millipore #1.06404) ...
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bioRxiv - Genetics 2024Quote: ... individuals were fixed for 1 hour in 1×PBS and 4% paraformaldehyde at room temperature, washed with BBT (1×PBS, 0.1% Tween 20 (VWR, ref:28829.296), 0.1% BSA (Euromedex ...
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bioRxiv - Bioengineering 2020Quote: ... bacteria were removed from media by centrifugation at 12,000g for 5 min and the supernatants were transferred to black flat-bottomed microtiter plates (VWR).
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bioRxiv - Bioengineering 2022Quote: ... 50/15 mL Falcon tubes and 50/5/2 mL DNA/Protein loBind Eppendorf tubes were purchased from VWR. D02-E100-05-N and C02-E100-05-N tangential flow filtration filters were purchased from Repligen ...
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bioRxiv - Microbiology 2021Quote: ... MD) and maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS, VWR) and 1% penicillin-streptomycin-glutamine (PSG ...
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bioRxiv - Synthetic Biology 2020Quote: ... centrifuged for 5 min at 4000 x g-1 and filtered over a 0.2 µm cellulose acetate filter (VWR). 1 ml aliquots were concentrated in a speed-vac (Eppendorf ...
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bioRxiv - Neuroscience 2020Quote: ... into the LC (AP:−5.4 mm, ML: ±1.2 mm, DV, −4.0 mm) with a 5 µL Hamilton syringe (VWR, Radnor, PA) and stereotaxic injector pump (Stoelting ...
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bioRxiv - Cancer Biology 2020Quote: ... through injection of 5×105 67NR murine luminal breast cancer cells in 100 μL of serum-free RPMI (VWR). 67NR cells are locally invasive but non-metastatic ...
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bioRxiv - Neuroscience 2020Quote: ... Leads were connected to the animal’s front paws and right hind paw via Skintact FS-TB1-5-Gel ECG Electrode (VWR) as shown in Figure 1.
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then blocked with 5% nonfat dairy milk in Tris-buffered saline containing 0.1% Tween 20 (VWR, 0777), and incubated overnight with the following primary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were blocked for 90 minutes at room temperature in a humidified chamber with a blocking buffer consisting of 0.1% Triton X-100 and 5% Bovine Serum Albumin (VWR). All the antibodies used in this study and their concentrations are listed in File S6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Later blocking was done for 1hour at RT on a rocker with a blocking buffer consisting of 0.1% Triton X-100 and 5% goat serum (VWR). The organoids were incubated overnight in the primary antibodies (File S6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Later blocking was done for 1hour at RT in a humidified chamber with a blocking buffer consisting of 0.1% Triton X-100 and 5% Bovine Serum Albumin (VWR). The organoids were incubated in primary antibodies (File S6 ...
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 5% (vol/vol) fetal bovine serum (FBS, VWR) and 1% penicillin–streptomycin–glutamine (PSG ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 million cells were seeded on a 15cm plate containing one 25 mm plastic coverslip (Thermanox, VWR 100500-878). At 24 hours after seeding ...
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a 1:5 volumetric ratio (chloroform:Trizol) for aqueous separation of RNA via centrifugation in heavy gel phase-lock tubes (VWR). Aqueous phase was transferred into isopropanol followed by RNA purification using a Qiagen RNeasy Mini Kit as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Benzonase nuclease was purchased from Sigma (E1014-25KU) and streptavidine sepharose high performance beads (5 mL) was purchased from VWR (GE Healthcare 17-5113-01). OMIX C18 tips (100 µL ...
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bioRxiv - Microbiology 2023Quote: ... XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] solution (0.5 mg XTT/mL 1× PBS with 25 µM menadione) (XTT sodium salt, VWR) was added at 150 µl per well and incubated at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... after which they were permeabilized in 0.5% Triton X-100 (MilliporeSigma) for 10 minutes and blocked with 5% bovine serum albumin (VWR) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µl of a blocking/permeabilization buffer (5% BSA and 0.2% Triton in PBS; BSA from Sigma; TritonX-100 from VWR) was added and cells were incubated for 1h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... TGIRT was dissociated from the RNA-DNA duplexes by addition of 1 μL 5 M NaOH (VWR BDH7225-1) with incubation at 95 °C for 3 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... serial dilutions were made for each suspension ranging from 10-3 to 10-7 and 100 μL of each of these were streaked on a 5% sheep’s blood in tryptic soy agar plate (VWR) and incubated at 37°C for 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... 2 ml of the culture was collected through centrifugation (5 min, 8000 rpm) and the supernatant filtered (0.22-μm filter; VWR). Secreted proteins in the supernatant were then precipitated using 10% ice cold trichloroacetic acid (TCA ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then blocked in a solution containing 5% skimmed milk powder in Tris-buffered saline with 0.1% Tween-20 (VWR) for 1 hour at ambient temperature.
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bioRxiv - Neuroscience 2024Quote: ... a 15%wt of CNT (<8 nm OD, 2–5 nm ID, length 0.5–2 µm, VWR, Inc., USA) was mixed with the PDMS ...
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bioRxiv - Bioengineering 2024Quote: ... approximately 5×106 cells were removed from the bioreactor and centrifuged at 200 x g for 2 min (VWR). The supernatant was discarded and the cell pellet was frozen in liquid nitrogen and stored at -80°C for further analysis.
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bioRxiv - Microbiology 2021Quote: ... The cells were washed off the filter with an ice-cold freshly prepared mixture of methanol/ethanol/chloroform (1:3:1) (ethanol LiChroSolv©, Merck, Darmstadt, Germany; chloroform HiPerSolv©, VWR, Dresden, Germany). Extracts were treated in an ultrasonic bath for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... Four sets of dilutions (100 to 10-3) of the filtered lysates were created in standard 96 well plates (VWR, cat. no. 25381-056), then arrayed in quadrants of a single 384-well plate ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and cells were resuspended in 65μL RNAase A solution consisting of 10 mg/mL RNAase A (VWR Life Science, 9001-99-4) dissolved in 10 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... Then the tissues were washed in PBS and stored at 4°C with 0.025% (w/v) sodium azide (VWR, Cat#786-299). For cryoprotection ...
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bioRxiv - Biophysics 2020Quote: VLPs suspended in PBS buffer were mixed at 4°C with KIF5B-spycatcher in (buffer) for 7-8 hours on a nutating mixer (VWR, Radnor, PA) to ensure Spy Tag-Spy Catcher binding ...
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bioRxiv - Neuroscience 2021Quote: ... Rats were then perfused intracardially with 100ml of 0.9% phosphate saline buffer (PBS) and 100ml of ice-cold 4% paraformaldehyde (PFA, BDH, Prolabo, VWR International, Lovaina, Belgium). Then ...
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bioRxiv - Physiology 2021Quote: ... Tissue samples were embedded in paraffin and sections of 4 μm were coated with 43–45°C Kodak NTB emulsion (VWR, Herlev, Denmark) diluted 1:1 with 43–45°C water ...
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bioRxiv - Neuroscience 2022Quote: ... Then incubated in 10% and 30% sucrose in PBS for 24 hr sequentially at 4 °C and embedded in the OCT compound (cat# 4583; VWR, Randor, PA). Coronal sections were cut at 20 or 50µm on a cryostat (CM3050S ...
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bioRxiv - Neuroscience 2022Quote: ... Rats were then perfused intracardially with 100 ml of 0.9% phosphate saline buffer (PBS) and 100 ml of ice-cold 4% paraformaldehyde (PFA, BDH, Prolabo, VWR International, Louvain, Belgium). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with retroviral Gag-pol (8 ug) and VSV-G (4 ug) packaging plasmids and pBabe vector (8 ug) using PEI Max transfection reagent (VWR, 75800-188). After 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... mice were acclimated in a custom clear acrylic behavioral chamber (4” x 2” x 6”) with a filter paper bottom (VWR #28321-113) for 10 minutes prior to intranasal treatment ...
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bioRxiv - Cell Biology 2022Quote: ... embedded in paraffin at least 12 hours and sliced (4 μm) using a Leica microtome on Superfrost Plus slides (VWR, 631-0108) prior to drying at 60°C for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The tumor and all the major organs (heart, lungs, spleen, kidney and liver) were fixed in 4% paraformaldehyde (PFA, Klinipath, VWR, PA, USA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The animals were sacrificed 72h after the Hypoxisense 680 measurement with Dolethal (Pentobarbital Sodico) (200 mg/ml, Vetoquinol, Aartselaar, Belgium) and the tumors were fixed in 4% paraformaldehyde (PFA, Klinipath, VWR, PA, USA). Only histological procedures were performed on these tumors.
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bioRxiv - Cell Biology 2023Quote: Human AT biopsies were washed twice in PBS to remove excess blood and divided in 50 to 100 mg for fixation in 4% PFA (paraformaldehyde, electron microscopy grade (VWR #100504-858)) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were mounted non-sequentially (such that sections on the same slide were at least 4 apart) onto SuperFrost Plus slides (VWR, Lutterworth, UK) and air- dried ...
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bioRxiv - Cancer Biology 2023Quote: ... we let the frozen slides reach –20 °C before transferring them directly to 1X PBS/4% paraformaldehyde (PFA) (VWR, cat. no. 10047105). We incubated the sections in PFA for 10 min at room temperature ...