Labshake search
Citations for VWR :
951 - 1000 of 1100 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Gryphon cells were cultivated according to manufacturer’s instructions at 5% CO2 37 °C in 10 cm tissue culture-treated dishes (VWR Canada, Mississauga, ON) in DMEM-high glucose (Life Technologies ...
-
bioRxiv - Cell Biology 2022Quote: ... The 40 μL solution of the lipid mixture was spread on a 5% w/v PVA film dried on a 25 x 25 mm coverslip (VWR, Radnor, PA) and then put under vacuum for at least 2 h to form a dry lipid film ...
-
bioRxiv - Molecular Biology 2022Quote: ... the medium was changed and the incubation continued for another 24 hours before addition of 1-5 µg/mL puromycin (VWR 97064-280). Incubation with puromycin continued for several days until untransfected control wells were depleted of cells ...
-
bioRxiv - Genomics 2021Quote: ... and myotubes from one 10 cm plate (∼5 million cells) with >90% viability were lifted as described above and pelleted in 15 mL polypropylene falcon tubes (VWR, 89039-670) by centrifuging for 5 minutes at 1500 RPM ...
-
bioRxiv - Cell Biology 2021Quote: Aggregation was induced by agitating 5 mg/mL α-synuclein at 37 °C for 7 days 1200 rpm (VWR, Cat#12620-942). Fibril formation was evaluated by electron microscopy (see next section for details) ...
-
bioRxiv - Physiology 2020Quote: ... 5-μm sections were stained with hematoxylin and eosin (H&E) using the Leica Autostainer and mounted in DePeX (VWR, Lutterworth, UK). Adipocyte diameter and pancreatic β-cell islet number and size were quantified using ImageJ software as previously described (7 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The RNA/DNA hybrid was tagmented in TD reaction buffer (10 mM Tris-Cl pH 7.6, 5 mM MgCl2, 10% DMF) supplemented with 3.4% PEG8000 (VWR Life Science, Cat.No.97061), 1 mM ATP (NEB ...
-
bioRxiv - Cell Biology 2021Quote: ... decalcification solution for 14 days and then paraffin embedded for sectioning with a Leica RM2235 microtome at 5 μm per section to generate histology slides (VWR 631-1553). Sections were processed and stained with TRAP solution and counterstained with 0.2% methyl green solution (Bioenno Lifesciences ...
-
bioRxiv - Biochemistry 2022Quote: ... Individual yeast colonies were picked into 4mL of pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter - 20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific, Radnor, PA), 5 g Casamino Acids (VWR) ...
-
bioRxiv - Biochemistry 2022Quote: The eluates of SILAC “heavy” and “light” were combined for each SA+ and SA-group respectively followed by reduction in 5 mM TCEP (VWR, #97064-848) for 1 hour and alkylation in 10 mM iodoacetamide (VWR ...
-
bioRxiv - Biophysics 2022Quote: ... peptides were resuspended in 20 μL of resuspension buffer (5:95 ACN:MilliQ H2O, 0.1% TFA) and solubilized in a bath sonicator (VWR, 35 kHz, 10 min). A ZipTip was placed on a P10 pipettor set to 10 μL ...
-
bioRxiv - Microbiology 2022Quote: ... 1 cell line was cultured at 37°C with 5% CO2 in DMEM with high glucose and L-glutamine (VWR 16777-129) supplemented with 10% heat inactivated FBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1-2 preps of in vitro transcribed RNA and 5-10µg N gene RNA were mixed with the prepared cell suspension in a 4mm electroporation cuvette (VWR International # 89047-210) and pulsed in a BioRad GenePusler using the exponential protocol with the following settings ...
-
bioRxiv - Microbiology 2022Quote: ... containing 1.5 ml of brain–heart infusion broth ([BHI]; Becton Dickinson, Franklin Lakes, NJ) supplemented with 0.1% Tween 80 (VWR Chemicals, Solon, OH) and were incubated at 37°C with 5% CO2 ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were put into 5’polystyrene chromatography columns with coarse filters (Evergreen, 208-3383-060) containing DEAE Sephadex A-25 resin (VWR, 95055-928). The columns were equilibrated in high salt buffer (20mM Tris-HCL ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were then loaded into 5’polystyrene chromatography columns with coarse filters (Evergreen, 208-3383-060) containing DEAE Sephadex A-25 resin (VWR, 95055-928). Once the sample completely flowed through the column ...
-
bioRxiv - Neuroscience 2023Quote: ... The PVDF membranes were blocked in 5% Nonfat dry milk (Bio- Rad Laboratories, Cat.# 1706404) in Tris-buffered saline with 0.1% Tween 20 (TBST) (VWR, Cat.# K873-4L). Samples were probed with the primary antibodies listed in Supplemental Table 1 overnight in 5% bovine serum albumin (Gold Biotechnology ...
-
bioRxiv - Genetics 2023Quote: ... 500μl of concentrated bacterial suspension or 5% (w/v) sucrose solution (mock treatment) was added to empty fly vials containing a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
-
bioRxiv - Biophysics 2023Quote: ... The 40 μL solution of the lipid mixture was spread on a 5% w/v PVA film dried on a 25 x 25 mm coverslip (VWR, Radnor, PA) and then put under vacuum for at least 2 h to form a dry lipid film ...
-
bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl, pH 7.2, 5 mM EGTA, 100 mM KCl, 1% Triton-X100; all chemicals from VWR; Radnor, PA, USA). Samples were homogenized using tight-fitting pestles and centrifuged at 3,000 g for 30 minutes at 4ºC ...
-
bioRxiv - Bioengineering 2024Quote: ... The cell suspension was then centrifuged at 300g for 5 mins and re-suspended in DMEM/F-12 with L-glutamine (VWR, Radnor, PA), 10% fetal bovine serum ...
-
bioRxiv - Bioengineering 2024Quote: ... The cells were then centrifuged at 300 x Gs for 5 min and the pellet was resuspended in DMEM/F-12 with L-glutamine (VWR, Radnor, PA), 10% fetal bovine serum ...
-
Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activitybioRxiv - Bioengineering 2024Quote: ... The sorted yeast cells were collected in a microcentrifuge tube and regrown in pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter - 20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific, Radnor, PA), 5 g Casamino Acids (VWR) ...
-
bioRxiv - Bioengineering 2024Quote: ... SIH5841.5), 1960 µL of deionized (DI) H2O (PURELAB Flex, ELGA LabWater) and 120 µL of 0.5 M HCl (VWR, catalog no. 20246.298). The solution was stirred vigorously for 1 hour ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were cultured in normal growth medium then switched to cysteine- and methionine-free medium DMEM supplemented with 2 mM L-glutamine (VWR) and 100 µM HPG (Invitrogen) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Ganglioid frozen sections and paraffin-embedded human normal colon sections were prepared as above and then washed three times in PBS and blocked for 1-2 hours in serum (10% donkey or 10% goat) with 0.5% (v/v) Triton X-100 (VWR 0694). Slides were then incubated with primary antibody diluted in serum (10% donkey or 10% goat ...
-
bioRxiv - Physiology 2020Quote: ... adi14-3-3ζKO mice were fasted for six hours and challenged with d-glucose (2 g/kg b.w.; VWR, Solon, OH) by intraperitoneal administration (34 ...
-
bioRxiv - Bioengineering 2020Quote: ... The PDMS slab was bonded to a 2-in by 3-in glass slide (VWR micro slides, cat. #48382-179) after plasma treatment (Harrick Plasma PDC-001 ...
-
bioRxiv - Bioengineering 2021Quote: ... An array of 2 mm holes was punched in the channel outlets using a biopsy punch (VWR, Cat# 21909-132). A hole was drilled on a plate lid (VWR ...
-
bioRxiv - Immunology 2020Quote: ... or Fc.IL-2 for 15 min at 37°C in complete RPMI (RPMI (Hyclone) supplemented with 10% FBS (heat inactivated, VWR), L-glutamine ...
-
bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int ...
-
bioRxiv - Genomics 2022Quote: ... The spore suspension was set to 2·108 spores mL-1 using a Bürker-Türk haemocytometer (VWR, Amsterdam, The Netherlands) and kept on ice until further use.
-
bioRxiv - Biophysics 2022Quote: ... Cells were allowed to recover for 48 hours before passage into new 10 cm plates containing DMEM media supplemented with 2 mg/ml Geneticin sulfate (G418; VWR). Following 7 days of selection ...
-
bioRxiv - Microbiology 2021Quote: ... The PDMS slab was bonded to a 2-in by 3-in glass slide (VWR micro slides, cat. #48382-179) after plasma treatment (Harrick Plasma PDC-001 ...
-
bioRxiv - Microbiology 2020Quote: A Ninjaflex 2 mm thick gasket was 3D printed onto a 50 mm × 75 mm × 1 mm glass slide (VWR) using a Makerbot 2× printer (Appendix I) ...
-
bioRxiv - Bioengineering 2021Quote: ... well plates were washed two times with MilliQ H2O and then stained with 400 μL of 2% w/V alizarin red S (VWR) for 10 min ...
-
bioRxiv - Microbiology 2021Quote: ... or lung homogenate at a concentration of 1×107conidia/ml in 2 ml of medium in glass 20ml disposable scintillation vials (VWR). Cultures were incubated at 37°C while shaking at 300 rpm ...
-
bioRxiv - Cell Biology 2020Quote: ... we fixed 250 μL of cells in 1000 μL of ice-cold methanol in a 2 mL plastic tube (16466-060, VWR). For wounded cells ...
-
bioRxiv - Microbiology 2021Quote: ... was added to each well of a 96-well AcroPrep filter plate with 0·2 μM wwPTFE membrane (514-1096, VWR). A100 μL of plasma sample ...
-
bioRxiv - Biophysics 2021Quote: ... The sonicator probe (SFX250 Sonifier with 1/2” horn and 3 mm tapered microtip, Branson Ultrasonics, VWR International, Radnor PA) was then positioned in the chamber with its tip 2 mm above the coverslip ...
-
bioRxiv - Microbiology 2020Quote: ... Transfections were conducted by electroporation using a BioRad Gene Pulser Xcell at 1.5 kV and 25 µF with 2 mm electroporation cuvettes (VWR international) in Cytomix buffer (10 mM KH2PO4/K2HPO4 (pH 7.6) ...
-
bioRxiv - Genetics 2020Quote: ... HEPES pH 7.8 and 0.00004% methylene blue in autoclaved reverse osmosis water) and mounted them on coverslip bridges (2 x 22 mm square glass coverslips (16004-094, VWR) glued together on either side of a 24 x 60 mm glass cover slip (12460S ...
-
bioRxiv - Neuroscience 2021Quote: ... slides were incubated with 0.25% Triton X for 20 min (in 1X TBST) and afterwards blocked with 2% normal donkey serum (NDS; VWR, USA) in 1X ...
-
bioRxiv - Biochemistry 2022Quote: ... an LD50 culture was prepared by pipetting 100 μL of the diluted expression culture into each cavity of a 96-deep well plate (96 square well, 2 mL, VWR) containing 400 μL of expression media (final OD600 = 0.02) ...
-
bioRxiv - Genomics 2022Quote: ... multimodal slides with leaf sections attached to the capture areas were incubated for 2 minutes at 37°C followed by a 40-minute fixation in methanol (VWR) at -20°C ...
-
bioRxiv - Immunology 2022Quote: ... Biotin+ or total DCs were sorted into U bottom 96well plate containing supplemented RPMI and 2% of T-stim media (VWR). 750 naïve CD4+ OT-II CFSE-labeled T cells from the spleen were sorted into the corresponding wells ...
-
bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Resistance selection using fixed concentrations of COV-siRNA1 or COV-siRNA1+2 was conducted in Vero E6 cells cultured in DMEM supplemented with 10% FBS (VWR) and 1x Penicillin/Streptomycin (Thermo Fisher Scientific) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Formic acid (FA), 2-proponol, acetonitrile (ACN), and methanol (all HiPerSolv CHROMANORM, HPLC grade, BDH Prolabo) were purchased from VWR International (Helsinki ...
-
bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...