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Citations for VWR :
801 - 850 of 1100 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Transgene expression was induced with 24-hour incubation in media supplemented with 2 μg/ml doxycycline (VWR). SiRNA-mediated depletion of endogenous ESCO2 was done with 20 nM siRNA (Dharmacon ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplified cPCR products were examined on 2% agarose gels stained with GelRed (VWR International PBI, Milan, Italy) and visualized on a GelLogic 100 gel documentation system (Kodak ...
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bioRxiv - Cancer Biology 2022Quote: ... for 2 hours at 56°C and 50 μg of RNase A (VWR International, Radnor, PA, USA) for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... spectinomycin (100 µg/ml) and 0.2% arabinose in a 2 ml 96-well deep well block (VWR), sealed with breathable film (VWR ...
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bioRxiv - Neuroscience 2020Quote: ... Cells used for in vitro electrophysiology experiments were grown on glass coverslips (12×12mm, No.2, VWR). Patch-clamp experiments were performed in aCSF consisting of (in mM) ...
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bioRxiv - Cancer Biology 2024Quote: ... or tissue culture-treated T-25 flasks (2×106 cells/flask; Avantor/VWR, Cat. No. 10861-568) under the growth conditions described above ...
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bioRxiv - Cell Biology 2023Quote: ... slabs were optically cleared using a 1:2 mixture of benzyl alcohol (Cat. No. 109626; VWR, Sweden) and benzyl benzoate (BABB ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 x 107 cells per replicate were resuspended in lysis buffer (8 M urea (VWR, 0568-500G), 50 mM Tris-HCl pH8.0 ...
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bioRxiv - Biophysics 2023Quote: ... were soaked for 1 hour at ~ 60°C in 2% v/v cleaning detergent (MICRO-90, VWR), thoroughly rinsed with 18.2 MΩ ultra-pure water ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µL cell suspension was loaded under a 22 x 22 mm glass coverslip (VWR, thickness: 1.5).
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bioRxiv - Systems Biology 2024Quote: ... Genomic DNA was then normalized to 2 ng/µL by diluting in molecular grade water (VWR International) using a Tecan Evo Liquid Handling Robot.
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bioRxiv - Systems Biology 2024Quote: ... Genomic DNA was then normalized to 2 ng/µL by diluting in molecular grade water (VWR International) using a Tecan Evo Liquid Handling Robot.
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bioRxiv - Microbiology 2024Quote: ... of the sample weight for 2 minutes at maximum speed using the Stomacher® 3500 (VWR, Italy). To assess the microbial load ...
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bioRxiv - Microbiology 2024Quote: ... centrifuged at 15,000 rpm for 2 min and filtered through a 0.2 μm cellulose acetate membrane (VWR). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... blood was washed out with 2 ml PBS and 1 ml of latex dye (VWR, LL0057-1L) was slowly and steadily injected into the left ventricle with an insulin syringe ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and cells were resuspended in 65μL RNAase A solution consisting of 10 mg/mL RNAase A (VWR Life Science, 9001-99-4) dissolved in 10 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... Then the tissues were washed in PBS and stored at 4°C with 0.025% (w/v) sodium azide (VWR, Cat#786-299). For cryoprotection ...
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bioRxiv - Biophysics 2020Quote: VLPs suspended in PBS buffer were mixed at 4°C with KIF5B-spycatcher in (buffer) for 7-8 hours on a nutating mixer (VWR, Radnor, PA) to ensure Spy Tag-Spy Catcher binding ...
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bioRxiv - Neuroscience 2021Quote: ... Rats were then perfused intracardially with 100ml of 0.9% phosphate saline buffer (PBS) and 100ml of ice-cold 4% paraformaldehyde (PFA, BDH, Prolabo, VWR International, Lovaina, Belgium). Then ...
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bioRxiv - Physiology 2021Quote: ... Tissue samples were embedded in paraffin and sections of 4 μm were coated with 43–45°C Kodak NTB emulsion (VWR, Herlev, Denmark) diluted 1:1 with 43–45°C water ...
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bioRxiv - Neuroscience 2022Quote: ... Then incubated in 10% and 30% sucrose in PBS for 24 hr sequentially at 4 °C and embedded in the OCT compound (cat# 4583; VWR, Randor, PA). Coronal sections were cut at 20 or 50µm on a cryostat (CM3050S ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: For egg laying small Petri dishes (4 cm diameter) were filled with a 3% agarose (VWR life science; type number: 97062-250), 3% sucrose (Merck KGaA ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were mounted non-sequentially (such that sections on the same slide were at least 4 apart) onto SuperFrost Plus slides (VWR, Lutterworth, UK) and air- dried ...
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bioRxiv - Cancer Biology 2023Quote: ... we let the frozen slides reach –20 °C before transferring them directly to 1X PBS/4% paraformaldehyde (PFA) (VWR, cat. no. 10047105). We incubated the sections in PFA for 10 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Rats were then perfused intracardially with 100 ml of 0.9% phosphate saline buffer (PBS) and 100 ml of ice-cold 4% paraformaldehyde (PFA, BDH, Prolabo, VWR International, Louvain, Belgium). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with retroviral Gag-pol (8 ug) and VSV-G (4 ug) packaging plasmids and pBabe vector (8 ug) using PEI Max transfection reagent (VWR, 75800-188). After 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... embedded in paraffin at least 12 hours and sliced (4 μm) using a Leica microtome on Superfrost Plus slides (VWR, 631-0108) prior to drying at 60°C for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The tumor and all the major organs (heart, lungs, spleen, kidney and liver) were fixed in 4% paraformaldehyde (PFA, Klinipath, VWR, PA, USA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The animals were sacrificed 72h after the Hypoxisense 680 measurement with Dolethal (Pentobarbital Sodico) (200 mg/ml, Vetoquinol, Aartselaar, Belgium) and the tumors were fixed in 4% paraformaldehyde (PFA, Klinipath, VWR, PA, USA). Only histological procedures were performed on these tumors.
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bioRxiv - Cell Biology 2023Quote: Human AT biopsies were washed twice in PBS to remove excess blood and divided in 50 to 100 mg for fixation in 4% PFA (paraformaldehyde, electron microscopy grade (VWR #100504-858)) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Cancer Biology 2024Quote: FFPE tissue blocks containing human primary tumors were sectioned at 4 mm onto Superfrost Plus microscope slides (VWR International, catalog 48311-703). Automated immunohistochemistry was performed at the Oncology Tissue and Imaging Services Core of the Johns Hopkins University School of Medicine using a Ventana Discovery Ultra automated slide staining system (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... overnight growth was prepared by inoculating three colonies into 4 mL GM in round-bottom clear polystyrene culture tubes (VWR, 734-0435) and incubated at 37°C with 200 rpm shaking for 18 hours and 6 days for M ...
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bioRxiv - Bioengineering 2024Quote: Following sacrifice of the animals at day 14 post-injection eyes were enucleated and fixed in 4% stabilized formaldehyde buffer (VWR, Søborg, Denmark) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... cells were maintained in Joklik’s modified Eagle’s minimal essential medium (US Biological, M3867) supplemented to contain 5% fetal bovine serum (FBS; VWR, 97068-085), 2 mM l-glutamine ...
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bioRxiv - Biophysics 2021Quote: ... The strains were first passed from agar plates (stored at 4oC) to 5 ml medium (round bottom polystyrene tubes, VWR) until early stationary phase ...
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bioRxiv - Immunology 2021Quote: ... 5% CO2 in a 96-well U-bottom plate (Falcon-Corning U-bottom tissue culture plates, VWR, Radnor, PA, USA). In conditions where rhCD80-Fc (Abatacept ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell suspension was filtered through a 35µm cell strainer into a 5 ml falcon round bottom tube (VWR #21008-948) for sorting using a MoFlo XDP cell sorter fitted with a 100µm nozzle ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% Tween20) and were fixed in egg buffer with 1% paraformaldehyde for 5 minutes on a Superfrost Plus slide (VWR). Gonads were then flash frozen in liquid N2 and the cover slip was removed ...
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bioRxiv - Cell Biology 2022Quote: ... The integrated vector backbone was removed by growth in non-selective YT media (5 g/l yeast extract (VWR, EM1.03753.0500), 5 g/l tryptone (Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... We washed the plate with PBST (Applied Cells, Inc.) five times and added 100 μl 5% BSA (VWR Life Sciences) into each well and incubated at 37°C for 90 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) and were mounted onto Superfrost Plus microscope slides (VWR) in VECTASHIELD antifade mounting medium (Vector Laboratories) ...
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bioRxiv - Genetics 2022Quote: ... and an equal volume of 1x Sperm Salts with 3% paraformaldehyde was applied for 5 min before samples were affixed to a Superfrost Plus slide (VWR). For both protocols ...
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bioRxiv - Genetics 2022Quote: ... 0.1% Tween20) and were fixed in 1x Egg Buffer with 1% paraformaldehyde for 5 min on a SuperFrost Plus slide (VWR). Slides were then flash frozen in liquid nitrogen and the cover slip was removed before the slides were placed in ice cold methanol for 1 minute ...
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bioRxiv - Genetics 2022Quote: ... 0.1% Tween20) and were fixed in 1x Egg Buffer with 1% paraformaldehyde for 5 min on a Superfrost Plus slide (VWR). Samples prepared for SMC-5::AID*::3xFLAG visualization were dissected in 1x Sperm Salts (50mM PIPES pH7 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... yeast cultures were diluted to OD600=0.67 in 5 mL SGDCAA (6.7 g/L YNB without amino acid VWR #90004-150), 5 g/L ammonium sulfate (Sigma-Aldrich #A4418) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 - 1 million gated events were collected into a 15 mL conical tube with 5 mL of LB supplemented with 1 % D-Glucose (10117, VWR) and without antibiotics ...
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Cannabinoids differentially modulate behavioral and developmental responses to ethanol in DrosophilabioRxiv - Pharmacology and Toxicology 2021Quote: ... Eight adult male flies were transferred into a vial containing 1% agarose with four 5 μl glass capillaries (VWR, USA). The capillaries were inserted from the top of the vial via adaptors made of pipette tips (He et al. ...
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bioRxiv - Physiology 2020Quote: ... containing 10% serum and centrifuged at 50 g for 5 min to enrich hepatocytes and passed through a 100-micron nylon cell strainer (10199-659, VWR) multiple times ...
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bioRxiv - Microbiology 2021Quote: L929 cells were grown in either suspension or monolayers in Joklik’s modified Eagle’s minimal essential medium (US Biological) supplemented to contain 5% fetal bovine serum (FBS; VWR, 97068-085). CHO cells were grown in Ham’s F12 medium (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... were washed with 5% HellmanexIII (Lactan, 105513203) in ≥18 MΩ MonoQ H2O at 65°C in a table top Incu-Line oven (VWR) for 4 h and then rinsed 10 times with ≥ 18 MΩ MonoQ H2O ...