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Citations for VWR :
751 - 800 of 1100 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... in antibiotic-free LB and 2 mL-deep-96-well plates in checkered pattern (VWR International). Plates were incubated at 37°C with 700 rpm shaking (Microplate Shaker TiMix 5 ...
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bioRxiv - Immunology 2020Quote: ... were separately stored in 2 mL pre-filled 2.4mm metal beads tubes (VWR International, Radnor, PA), and quickly snap-frozen in liquid nitrogen (40) ...
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bioRxiv - Neuroscience 2024Quote: ... pre-chilled 2 ml Pre-Filled Bead Tubes with 2.8 mm Ceramic Beads (VWR, Radnor, PA), and weighed ...
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bioRxiv - Cell Biology 2022Quote: ... glucose was replaced with 2% (w/v) galactose (ITW reagents) or 1.5% (w/v) raffinose (VWR), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... mice expressing hM4D(Gi) were intraperitoneally injected with 2 mg/kg CNO (89160; VWR International, LLC) and returned to their home cages ...
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bioRxiv - Microbiology 2024Quote: ... 2 mL of DMEM infection media containing 0.2% w/v bovine serum albumin (BSA; VWR International) and 1 μg/mL L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... with media changes every 2-3 days before expansion to a T-75 flask (CellStar, VWR).
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bioRxiv - Cell Biology 2024Quote: ... 2 μl cell suspension was loaded under a 22 × 22 mm glass coverslip (VWR, thickness: 1.5). Fluorescence images in Figures 1b ...
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bioRxiv - Microbiology 2021Quote: ... ‘Fast’ shaking was established by orbital rotation of flasks at setting 4 on an orbital shaker (Model 3500, VWR, Radnor, PA), which corresponds to ∼200 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrigel containing organoid samples were removed from multi-well tissue culture dishes (using a 3.5 mL disposable transfer pipette and placed in 45 mL of cold PBS (4°C) in 50 mL capacity polypropylene centrifuge tubes (VWR Scientific). Matrigel was washed away by 3-4 rounds of suspension in cold PBS and centrifugation using a refrigerated centrifuge set to 1,000 pm ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted at 5,000 rpm for 15 minutes at 4°C and resuspended in 10 mL of lysis buffer [300 mM NaCl (VWR BDH9286), 20mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... mice were deeply anaesthetized with isoflurane (4% flow rate of 0.5–0.7 l/min) and transcardially perfused with cold phosphate-buffered saline (PBS) followed by 4% PFA (VWR International Inc, 15710). Brains were extracted and placed in PFA for 24 h and then transferred to 30% sucrose/PBS solution at 4 °C ...
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bioRxiv - Physiology 2023Quote: ... the sulfhydryl groups were derivatized by transferring the final supernatant to 1.55 M NaOH and Na2B4O7·10H2O (125 mM with 4 mM EDTA, pH 9.5, VWR, Radnor, PA, USA) solutions ...
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bioRxiv - Bioengineering 2023Quote: ... conditioned media was centrifuged at 3000 RPM for 10 minutes at 4°C and the supernatant was filtered using 0.2 μm syringe filter (VWR, 28143-310). Sample placed in ultracentrifuge tubes (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... the transgenic gametocytes were pelleted at 1,400 xg for 3 minutes and fixed in 4% v/v paraformaldehyde (VWR, Cat# PI28908) and 0.0075% v/v glutaraldehyde (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... After overnight plating cells were treated with different doses of CDDP for 24 hours or with MMS for 8 hours and fixed with 4% PFA (VWR, AA47377) for 20 minutes and stored in PBS at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... tubes with mAb were incubated on a rotator at 4 °C overnight on a rotator (Model 13916- 822, VWR, United States). These mAb included anti-histone H3 (1:50 ...
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bioRxiv - Physiology 2023Quote: ... between the starting and ending levels of the removed capillary tubes were measured using a digital caliper (Traceable Carbon Fiber Caliper-4″, VWR International) to determine the volume consumed (after adjusting for evaporative losses) ...
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bioRxiv - Molecular Biology 2024Quote: ... overnight at 4°C followed by a goat anti-rabbit IgG secondary antibody conjugated to HRP (VWR, cat. no. 102645-182). F1β signal was then captured using the same method.
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bioRxiv - Immunology 2024Quote: ... Plaque assays were developed at 4 days post infection by removal of overlay medium and staining/fixation using 10% neutral buffered formalin (VWR, #89370) and 0.25% crystal violet (VWR ...
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bioRxiv - Biophysics 2024Quote: ... at 4°C overnight in a 35 mm Mat-Tek glass coverslip bottom dish (VWR; Catalog No. P35G-0-14-C). After the poly-D lysine coating ...
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bioRxiv - Cell Biology 2024Quote: ... cells were harvested and sonicated with a Branson SFX250 Sonifier with 102-C Converter and a 3/4" High-Gain Horn in cold lysis buffer containing 50 mM sodium phosphate-NaOH pH 7.5 (Oakwood #099682, VWR #97064-480), 150 mM NaCl (Millipore #1.06404) ...
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bioRxiv - Genetics 2024Quote: ... individuals were fixed for 1 hour in 1×PBS and 4% paraformaldehyde at room temperature, washed with BBT (1×PBS, 0.1% Tween 20 (VWR, ref:28829.296), 0.1% BSA (Euromedex ...
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bioRxiv - Bioengineering 2020Quote: ... bacteria were removed from media by centrifugation at 12,000g for 5 min and the supernatants were transferred to black flat-bottomed microtiter plates (VWR).
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bioRxiv - Microbiology 2021Quote: ... MD) and maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS, VWR) and 1% penicillin-streptomycin-glutamine (PSG ...
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bioRxiv - Synthetic Biology 2020Quote: ... centrifuged for 5 min at 4000 x g-1 and filtered over a 0.2 µm cellulose acetate filter (VWR). 1 ml aliquots were concentrated in a speed-vac (Eppendorf ...
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bioRxiv - Neuroscience 2020Quote: ... into the LC (AP:−5.4 mm, ML: ±1.2 mm, DV, −4.0 mm) with a 5 µL Hamilton syringe (VWR, Radnor, PA) and stereotaxic injector pump (Stoelting ...
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bioRxiv - Cancer Biology 2020Quote: ... through injection of 5×105 67NR murine luminal breast cancer cells in 100 μL of serum-free RPMI (VWR). 67NR cells are locally invasive but non-metastatic ...
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bioRxiv - Neuroscience 2020Quote: ... Leads were connected to the animal’s front paws and right hind paw via Skintact FS-TB1-5-Gel ECG Electrode (VWR) as shown in Figure 1.
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bioRxiv - Molecular Biology 2023Quote: ... Benzonase nuclease was purchased from Sigma (E1014-25KU) and streptavidine sepharose high performance beads (5 mL) was purchased from VWR (GE Healthcare 17-5113-01). OMIX C18 tips (100 µL ...
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bioRxiv - Microbiology 2023Quote: ... XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] solution (0.5 mg XTT/mL 1× PBS with 25 µM menadione) (XTT sodium salt, VWR) was added at 150 µl per well and incubated at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Plates were then washed 5 times in PBS before addition of 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) substrate (VWR), and incubation at 37°C for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µl of a blocking/permeabilization buffer (5% BSA and 0.2% Triton in PBS; BSA from Sigma; TritonX-100 from VWR) was added and cells were incubated for 1h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... after which they were permeabilized in 0.5% Triton X-100 (MilliporeSigma) for 10 minutes and blocked with 5% bovine serum albumin (VWR) for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... TGIRT was dissociated from the RNA-DNA duplexes by addition of 1 μL 5 M NaOH (VWR BDH7225-1) with incubation at 95 °C for 3 minutes ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then blocked with 5% nonfat dairy milk in Tris-buffered saline containing 0.1% Tween 20 (VWR, 0777), and incubated overnight with the following primary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were blocked for 90 minutes at room temperature in a humidified chamber with a blocking buffer consisting of 0.1% Triton X-100 and 5% Bovine Serum Albumin (VWR). All the antibodies used in this study and their concentrations are listed in File S6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Later blocking was done for 1hour at RT on a rocker with a blocking buffer consisting of 0.1% Triton X-100 and 5% goat serum (VWR). The organoids were incubated overnight in the primary antibodies (File S6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Later blocking was done for 1hour at RT in a humidified chamber with a blocking buffer consisting of 0.1% Triton X-100 and 5% Bovine Serum Albumin (VWR). The organoids were incubated in primary antibodies (File S6 ...
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 5% (vol/vol) fetal bovine serum (FBS, VWR) and 1% penicillin–streptomycin–glutamine (PSG ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 million cells were seeded on a 15cm plate containing one 25 mm plastic coverslip (Thermanox, VWR 100500-878). At 24 hours after seeding ...
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a 1:5 volumetric ratio (chloroform:Trizol) for aqueous separation of RNA via centrifugation in heavy gel phase-lock tubes (VWR). Aqueous phase was transferred into isopropanol followed by RNA purification using a Qiagen RNeasy Mini Kit as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... serial dilutions were made for each suspension ranging from 10-3 to 10-7 and 100 μL of each of these were streaked on a 5% sheep’s blood in tryptic soy agar plate (VWR) and incubated at 37°C for 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then blocked in a solution containing 5% skimmed milk powder in Tris-buffered saline with 0.1% Tween-20 (VWR) for 1 hour at ambient temperature.
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bioRxiv - Microbiology 2024Quote: ... 2 ml of the culture was collected through centrifugation (5 min, 8000 rpm) and the supernatant filtered (0.22-μm filter; VWR). Secreted proteins in the supernatant were then precipitated using 10% ice cold trichloroacetic acid (TCA ...
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bioRxiv - Genetics 2021Quote: ... Amplified emulsion mixture was broken and purified by adding 1 mL of 2-butanol (VWR, AA43315-AK), 50 μL of AMPure XP SPRI (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...