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Citations for VWR :
651 - 680 of 680 citations for Fumarase from porcine heart since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl, pH 7.2, 5 mM EGTA, 100 mM KCl, 1% Triton-X100; all chemicals from VWR; Radnor, PA, USA). Samples were homogenized using tight-fitting pestles and centrifuged at 3,000 g for 30 minutes at 4ºC ...
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bioRxiv - Neuroscience 2024Quote: ... an unsorted sample was taken from the input chamber of the cartridge using a 200 µl gel loading tip (VWR, 732-2671). The sort was performed in the MACSQuant Tyto cell sorter using software 1.0.0.
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bioRxiv - Neuroscience 2024Quote: ... Flies were then mechanically sleep-deprived 3 sec every min from ZT12-ZT0 using a vortexer (VWR DVX-2500 Multi-tube vortexer). Flies were then taken out of the individual tubes between ZT23 and ZT0 and placed into standard imaging chambers using a mouth pipette ...
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bioRxiv - Molecular Biology 2024Quote: ... SC-islets were then shipped from Vancouver to Ottawa and maintained in Stage 6 media [CMRL-1066 (VWR, #CA45001-114; Radnor, PA) containing 20 g/L bovine serum albumin (Sigma-Aldrich #10775835001) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell harvesting involved a PBS wash followed by 1 mL of a homemade lysis buffer containing a base buffer (50 mM Tris-HCl in DEPC-treated water, pH 7.4; 250 mM KCl; 25 mM MgCl2; all ingredients from VWR, Radnor, PA, USA) and the following chemicals ...
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bioRxiv - Immunology 2024Quote: Single cells prepared from tumor tissues were counted and 2-5 million cells were plated on a 96-well plate (VWR, round botom) and washed with PBS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The active fractions from the NC-MT assay were separated in a 10% SDS gel and stained with coomassie blue (InstantBlue, VWR Chemicals, Darmstadt, Germany) or silver stain kit for mass spectrometry (Pierce/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Bone marrow was collected via centrifugation from the femur and tibia of 8-week-old male C57BL6/J mice and cultured in αMEM (VWR, Radnor, PA, USA) supplemented with 10% fetal bovine serum VWR ...
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bioRxiv - Cell Biology 2020Quote: Concentration and quality of total RNA isolations using a phenol-chloroform extraction with Isol-RNA lysis reagent (5’Prime, Hamburg, Germany) from endothelial cells were verified by a NanoDrop™ 1000 (Peqlab VWR, Radnor, PA) and an Agilent 2100 bioanalyzer (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... Membranes that were electrospun from 15 wt% of PVDF without dyes onto a layer of PP fabrics (VWR® Basic Protection Face Mask) were also fabricated for comparison (i.e. ...
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bioRxiv - Microbiology 2020Quote: ... Synthesis of the first-strand cDNA from the extracted total RNA was conducted using a QuantaBio qScript cDNA synthesis kit (VWR Canada, Edmonton, AB). Primers and gBlocks were synthesized by Integrated DNA Technologies (Coralville ...
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bioRxiv - Microbiology 2021Quote: GC-376 (CAS: 1416992-39-6) was purchased from Enovation chemicals (Green book, NY) and dissolved in deionized water (HyClone, VWR, West Chester, PA) to the desired concentration.
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bioRxiv - Microbiology 2020Quote: ... We washed the pelleted biomass 1 – 2 times and resuspended it with 50 µL 1x PBS from which we fixed 2 µL on solidified agarose (VWR, Solon, OH, USA) (1% w/v) ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants collected from aliquots in the CFU growth arrest assay described above were filtered with 0.45um Supor® Membrane filters (VWR, Cat# 28143-352). 1:10 serial dilutions were carried out and 2ul of each dilution was plated onto the top agar containing RN4220 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... A single colony of bacteria from each plate was cultured under anaerobic conditions (Anaerobic Hungate Culture Tubes with air displaced by CO2, VWR, Radnor, PA, USA), shaking at 35°C for 3 nights and was then retained at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted from the eluted beads with phenol: chloroform: iso–amyl alcohol (25:24:1) (v/v/v) (VWR, 136112– 00–0), followed by precipitation with 1:1 (v/v ...
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bioRxiv - Physiology 2023Quote: ... Each brain tissue was carefully collected from the cranium individually and snap-frozen in a 1.5 mL microcentrifuge tube (VWR International, Radnor, PA, USA) chilled with liquid nitrogen ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were stimulated with p25 peptide-pulsed (sequence: FQDAYNAAGGHNAVF from Genescript) iBMDMs or FLAMs that were irradiated with mitomycin (25 µg/mL) (VWR, Cat no. TCM2320) and had been pre-treated with DMSO ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... Transwells were removed from their supports with a scalpel and mounted on glass slides with VECTASHIELD® Antifade Mounting Medium (VWR, VECTH-1000). Images were acquired with a Carl Zeiss LSM 510 Confocal Laser Scanning Microscope (40× magnification ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was extracted from lysed samples using the Quick Extract DNA Extraction Solution (Catalog: QE09050, Bio-Search Technologies, VWR, Radnor, PA, USA), following the protocol provided by the manufacturer ...
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bioRxiv - Genetics 2024Quote: ... Chondrocytes were given 4 days of recovery from digestion and maintained in cell culture medium consisting of DMEM/F12 supplemented with 10% FBS (VWR Seradigm; #97068-085) before experiments ...
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bioRxiv - Genomics 2024Quote: Serial sections of 10 μm thickness were obtained from the frozen tissue samples and mounted onto glass microscope slides (VWR SuperForst 48311-703). Using a Leica ST5010 Autostainer XL (Leica Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads are removed from glass and resuspended in TE-TW (TE buffer: Sigma-Aldrich, Inc., 8910-1L; Tween-20: VWR International, LLC, 100216-360) and subjected to 2 TE-TW washes followed by centrifugation for 2min at 3000rcf ...
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Exploring the interspecific interactions and the metabolome of the soil isolate Hylemonella gracilisbioRxiv - Microbiology 2022Quote: ... was added to the surface of the 1/10th TSBA plates and grown bacterial cells were suspended from the plate surface with a disposable cell scratcher (VWR international B.V., the Netherlands). For total RNA extraction the obtained cell suspension was transferred to a tube containing RNA Protect Bacteria Reagent (Qiagen ...
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bioRxiv - Bioengineering 2022Quote: ... Component 1 contains fluidic access ports to the underside channel that create sealed fits against P20/P200 pipette tips purchased from VWR (76322-516, Radnor, PA).
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bioRxiv - Developmental Biology 2021Quote: ... Transfected cells from 2 wells (technical duplicates) were seeded into 250 mL cell culture flasks with vented caps (10062-860, VWR International, Brisbane, CA, USA).
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bioRxiv - Evolutionary Biology 2022Quote: ... Single colonies were grown overnight in 10 mL YPGal+Dex and genomic DNA was isolated from 1 mL aliquots using the VWR® Life Science Yeast Genomic DNA Purification Kit (VWR 89492-616, us.vwr.com).
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bioRxiv - Synthetic Biology 2022Quote: ... 1 μL volumes are taken from this tray using a multipipettor and used to inoculate wells of a 96-well plate (VWR, catalog number 10062-900) with 200μL volumes of H media ...
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bioRxiv - Biophysics 2020Quote: ... Confocal Microscopy—HeLa cells grown in 35 mm imaging dishes (made in-house from Corning 35 x 10 mm dishes with VWR 18 x 18 mm #1.5 cover slips) were imaged 48 hours post transfection to maintain consistency with the FACS measurements ...
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bioRxiv - Biophysics 2020Quote: ... U2OS cells were grown in 35 mm imaging dishes (made in-house from Corning 35 x 10 mm dishes with VWR 18 x 18 mm #1.5 cover slips). All CyTERM constructs were transiently transfected for 18–24 hours using Lipofectamine 3000 (Invitrogen ...