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Citations for VWR :
501 - 550 of 680 citations for Fumarase from porcine heart since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The embedded organoids were then removed from the Parafilm and placed in a deep bottom 10cm plate (VWR) in COD – vitamin A media ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by density gradient centrifugation with Ficoll (VWR International) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... vimentin null (V-/-) MEF nuclei were isolated from living cells with 0.05% Triton X-100 (VWR Life Science) in PBS ...
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bioRxiv - Biophysics 2024Quote: All solvents and reagents were purchased from commercial sources (Sigma-Aldrich, VWR, GoldBio, EMD, P3 Bio Systems, etc.) and used without further purification ...
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bioRxiv - Microbiology 2024Quote: ... bile was obtained from the gallbladder with a 31 G short-needle insulin syringe (Sol-M®, VWR) and aspirated into 90 μl sterile PBS ...
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bioRxiv - Microbiology 2024Quote: Total DNA was extracted from feces or biofilms using the EZNA isolation kit stool DNA (VWR for biofilms) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... We extracted DNA from stable cultures of resident communities using peqGOLD Bacterial DNA Kit (VWR #13-3450-02), then sent the obtained DNA to Eurofins for library preparation and sequencing of 16S rDNA V3-V4 region ...
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bioRxiv - Systems Biology 2024Quote: ... were curated from E.coli DH5a using a plasmid extraction kit (peqGOLD Plasmid MiniPrep Kit II; VWR, Leuven, Belgium). PA01 cells were made competent for transformation by treatment with a sucrose 300 mM solution PA01 ...
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bioRxiv - Neuroscience 2021Quote: ... the samples were resolubilized with 15 min sonication in 30 μL of 1% acetonitrile + 0.1% trifluoroacetic acid in LC-MS grade water (all from VWR). The injection volume (between 2 and 10 μL ...
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bioRxiv - Molecular Biology 2020Quote: ... Peripheral blood was drawn from mice by retro-orbital sinus puncture using heparinized micro hematocrit capillaries (VWR, Darmstadt, Germany) and plasma separated and stored at −80 °C until further analysis ...
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bioRxiv - Immunology 2021Quote: Single-cell leukocyte suspensions were prepared from spleens by mechanical homogenization of tissues between frosted glass microscope slides (VWR) and filtration through a 70µm nylon mesh ...
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bioRxiv - Bioengineering 2021Quote: ... CQ loaded nanocarriers were purified from free CQ by 10K MWCO polyethersulfone membrane spin columns (VWR; Radnor, PA, USA) at 10,000 xg for 1-2 minutes and equilibrated with PBS solution.
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bioRxiv - Molecular Biology 2020Quote: ... 67-63-0, >96% purity) and chloroform (99-99.6%, stabilized with 0.6% ethanol) used for RNA extraction from VWR (Denmark). MilliQ water was purified on a PURELAB Chorus system (ELGA LabWater ...
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bioRxiv - Neuroscience 2020Quote: ... Blood from trunk was rapidly collected in BD vacutainer tubes containing EDTA as anticoagulant (VWR, Ville Mont-Royal, Quebec).
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bioRxiv - Neuroscience 2022Quote: ... the cell strainer was removed from the testing arena and dropped into a 6-well dish (VWR 10861-554) containing 4% PFA in 1x PBT (1x PBS + 0.25% TritonX100) ...
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bioRxiv - Genetics 2022Quote: ... leaf tissues from around 200 survivors were individually collected into 1.5-mL microtubes (VWR International LLC, Radnor, PA, USA) and stored at −80 ◻ until processing ...
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bioRxiv - Bioengineering 2022Quote: ... 50/15 mL Falcon tubes and 50/5/2 mL DNA/Protein loBind Eppendorf tubes were purchased from VWR. D02-E100-05-N and C02-E100-05-N tangential flow filtration filters were purchased from Repligen ...
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bioRxiv - Systems Biology 2022Quote: ... we isolated the growth media from liquid cultures by flowing liquid cultures through a 0.2 µm pore filter (VWR). We ensured that no cells remained in the filtered media by flowing an aliquot through our flow cytometer ...
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bioRxiv - Microbiology 2021Quote: ... The sample is entered and removed from the mixing chamber based on silicone tubing (internal diameter: 0.5 mm; external diameter: 1.6 mm, VWR, Belgium) and peristaltic pumps (400FD/A1 OEM-pump ~13 rpm and 290 rpm ...
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bioRxiv - Biochemistry 2021Quote: Custom Eppendorf tubes for extract recovery were created by cutting approximately 3 mm from the bottom of a standard 0.5 mL Eppendorf tube (VWR). The tubes were then soaked in 70% ethanol to remove small molecule contaminants ...
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bioRxiv - Neuroscience 2023Quote: ... all odors in this study were presented from a 10-3 (0.1%) dilution in paraffin oil (J.T. Baker, VWR #JTS894), with the exception of movies collected from one fly ...
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bioRxiv - Molecular Biology 2023Quote: ... Benzonase nuclease was purchased from Sigma (E1014-25KU) and streptavidine sepharose high performance beads (5 mL) was purchased from VWR (GE Healthcare 17-5113-01). OMIX C18 tips (100 µL ...
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bioRxiv - Bioengineering 2023Quote: ... and sulfuric acid (95-98%; BDH3068-500MLP) and nitric acid (69-70%; BDH3044-500MLPC) were purchased from VWR (PA). The dialysis membrane tubing (MWCO ...
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bioRxiv - Neuroscience 2023Quote: ... Blood for each animal was collected from the dorsal artery using 2-3 heparin coated capillary tubes (VWR®).
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bioRxiv - Physiology 2023Quote: Post-ultracentrifugation supernatant (S2) from brain lysates were thawed on ice alongside 1 mM Thioflavin T (VWR 103802-652). Thioflavin T (ThT ...
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bioRxiv - Microbiology 2023Quote: ... from pre-cultures were taken by a Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader (VWR, cat # 89429-536). Pre- cultures were diluted to a starting OD600 of 0.02 in 5 mL 0.1x R2A ...
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bioRxiv - Neuroscience 2022Quote: ... culture media for isolated astrocytes derived from doubly transgenic TDP43-ΔNLS mice contained tetracycline-depleted FBS (VWR #97065-310) that was heat-inactivated for 30 min at 56°C in a water bath ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Microbiology 2024Quote: ... and 100 µL of supernatant from each culture was transferred to either 96-well clear plates (VWR, #10861-562) for pyocyanin quantitation or 96-well black plates (Greiner ...
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bioRxiv - Bioengineering 2024Quote: ... The pH was monitored starting from 6.49 and reducing to 3.02 using a digital pH meter (accuracy ± 0.1% ± 2 digits, VWR pH110, Belgium).
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bioRxiv - Neuroscience 2024Quote: ... was added to 2ml tubes containing Precellys Steel beads (2.8 mm ‘reinforced’ 2 ml tubes, pre-filled with steel beads purchased from VWR International Ltd ...
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bioRxiv - Microbiology 2024Quote: ... Individual colonies from the overnight culture were resuspended in 1X PBS buffer saline (E504-100ML, VWR Life Science, USA), and the optical density of each suspension was measured at 600 nm using a UV 3100PC Spectrophotometer (VWR Life Science ...
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bioRxiv - Biochemistry 2024Quote: ... were transferred to a 5 mL or 10 mL round bottom flasks manufactured from borosilicate 3.3 glass with a 14/20 Joint size (VWR) and dried to a film by streaming N2 ...
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bioRxiv - Molecular Biology 2023Quote: ... TGIRT was dissociated from the RNA-DNA duplexes by addition of 1 μL 5 M NaOH (VWR BDH7225-1) with incubation at 95 °C for 3 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... we used a brush to release the eggs from the agar and transferred them to a cell strainer with 100µm nylon mesh (VWR). To remove the chorion ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 µm sections were cut from the OCT blocks and mounted on positively charged slides (VWR superfrost Plus slides). The slides were then stored at -80° C until use.
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bioRxiv - Cancer Biology 2022Quote: ... HT-1080 cells were purchased from ATCC and cultured with DMEM supplemented with 10% fetal bovine serum and 0.1% Gentamycin (VWR). SUM149 (SUM149PT ...
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bioRxiv - Cell Biology 2022Quote: ... The sample chamber for the cell adhesion experiment is a custom-made flow chamber that consists of a 26 mm x 76 mm coverglass slide and a 1.5 thickness coverslip from VWR glued together by two melted stripes of parafilm ...
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bioRxiv - Bioengineering 2022Quote: The final microfluidic device was made by assembling the PDMS channel and the PDMS-coated glass slide (75 x 51 mm, 1.2 mm thick, purchased from VWR). The reason for using a PDMS-coated glass slide instead of the glass slide itself is because PDMS decreases the wettability of internal phase ...
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bioRxiv - Bioengineering 2023Quote: The cell culture plate inserts (transwells) for 24 wells (PET membrane, 3.0 µm pore size) were purchased from VWR International.
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bioRxiv - Microbiology 2023Quote: ... The virus containing band was extracted from the gradient and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Physiology 2024Quote: ... using a FastPrep 24 (M.P. Biomedicals, Santa Ana, CA, USA) with bead-beating tubes from (VWR, Radnor, PA, USA) and zirconium ceramic oxide beads from (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... We prepared E-media by mixing Dulbecco’s Modified Eagle Medium (DMEM) with Ham’s F12 3:1 and supplement with 10% Fetal bovine serum (FBS, from VWR), 0.5% Penicillin/Streptomycin Solution,10 µl of 25 µg/µl Hydrocortisone 16.8 µl of 0.25 µg/µl Cholera toxin ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl, pH 7.2, 100 mM KCl, 20% glycerol, 1 mM DTT, 50 mM spermidine; all chemicals from VWR), and stored at -80°C for analyses described below.
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bioRxiv - Neuroscience 2024Quote: Brains from Experiment 5 were cut into coronal slices of 16 μm using a cryostat (from bregma +1.70 to +0.70, (Paxinos and Watson, 2013)) and subsequently mounted on SUPERFROST® Plus microscope slides (VWR). 2 slices per rat (4 x NAc ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from lysed tachyzoites using the Zymo Quick-RNA MiniPrep kit (VWR, cat. no. 76020-636). RT-qPCR was conducted using primers P20 and P21 for TgIF1 ...
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bioRxiv - Microbiology 2024Quote: ... Aliquots (20 µl each) from CCS samples were added to 96-well black plates (VWR, West Chester, PA, USA), and 50 µl of SteadyGlo luciferase reagent (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... myofibril pellets were resuspended in 300 μL of ice-cold wash buffer (Buffer 2: 20 mM Tris-HCl, pH 7.2, 100 mM KCl, 1 mM DTT; all chemicals from VWR), and tubes were centrifuged at 3,000 g for 10 minutes at 4ºC ...
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bioRxiv - Bioengineering 2024Quote: ... The media collected from 3 flasks (25mL/T-175flask) was filtered through a 0.2μm filter (VWR, Cat#10040-464) followed by first round of ultracentrifugation (Thermo Scientific™ Sorvall™ WX ultraCentrifuges ...
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bioRxiv - Bioengineering 2024Quote: ... Citric acid monohydrate (Cat. No. 97062-512) and tri-sodium citrate dihydrate (Cat. No. BDH9288) were purchased from VWR. 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI) ...