Labshake search
Citations for VWR :
551 - 600 of 680 citations for Fumarase from porcine heart since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from the enrichment beads using IBI scientific TRI-Isolate RNA Pure Kit (VWR cat# 76006-526) and eluted into 50 μL nuclease-free water ...
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bioRxiv - Bioengineering 2024Quote: ... approximately 5×106 cells were removed from the bioreactor and centrifuged at 200 x g for 2 min (VWR). The supernatant was discarded and the cell pellet was frozen in liquid nitrogen and stored at -80°C for further analysis.
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bioRxiv - Biophysics 2024Quote: ... All electrolyte solutions used in bilayer experiments were buffered with 10 mM phosphate and filtered through 0.22 µm pore size PES vacuum filters from VWR International (Radnor ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg RNA from each condition was used to generate cDNA with qScript cDNA SuperMix (VWR Cat#101414-104) according to manufacturing protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... Single-cell suspensions of immune cells from the liver were obtained by mechanical disaggregation through a 70µm cell strainer (VWR) and washed through with 10% FBS in RPMI ...
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bioRxiv - Biophysics 2021Quote: ... The strains were first passed from agar plates (stored at 4oC) to 5 ml medium (round bottom polystyrene tubes, VWR) until early stationary phase ...
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bioRxiv - Cell Biology 2020Quote: 150 µL of extract and 500 µL of 25 mM OPD in 2M HCl (made from o-Phenylenediamine, 98%; VWR) were combined ...
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bioRxiv - Cell Biology 2020Quote: ... This step was repeated twice and the supernatant collected were vacuum centrifuged at 30°C for 45 min Samples were then reconstituted in 64 µL of 200 mM ammonium acetate (made from; ammonium Acetate, 98%; VWR) and transferred to amber glass UPLC vials (Waters) ...
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bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
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bioRxiv - Cell Biology 2020Quote: ... immediately prior (within 10 seconds) to two 400 ms sonication pulses from a Branson Sonifier 450 (VWR International, 47727-492) with a 1/8” tapered microtip (VWR International ...
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bioRxiv - Microbiology 2020Quote: ... thetaiotaomicron 7330 tdk-strain was generated by UV mutagenesis by exposing a liquid culture of 7330 to 320 nm ultraviolet light from a VWR-20E transilluminator (VWR) for 60 seconds and plating onto BHIS-Blood agar supplemented with 200 micrograms/mL of 5-fluoro-2’-deoxyuridine (FUdR) ...
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bioRxiv - Synthetic Biology 2020Quote: Constructs were inoculated from -80C freezer stocks into 750 µL of appropriate media in 96-deep-well plates (VWR International) and incubated at 37 °C for 36 hours with shaking at 250 rpm ...
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bioRxiv - Cancer Biology 2021Quote: The 501Mel human melanoma line (Gift from Dr. Boris Bastian, CVCL_4633) were cultured in RPMI with 10% Fetal Bovine Serum (FBS, VWR, S107G), 1% Penicillin-Streptomycin (Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... The 40 O-rings were made from the PDMS sheet with a 10 mm puncher (VWR, Cat# CA-95039-098) for the outer perimeter and a 2 mm biopsy puncher for the inner perimeter ...
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bioRxiv - Bioengineering 2021Quote: ... sorted RBCs were removed from the microfluidic device and transferred to a 96-well flat-bottom plate (VWR International, LLC). Samples of sorted cells from each outlet were divided evenly and placed into two separate wells to provide cell images captured with variations in well location distribution and automatically determined imaging parameters (e.g ...
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bioRxiv - Microbiology 2020Quote: 250 μL of virus transport medium (VTM) from flocked NP swabs were adjusted to 1.0 mL with 0.1% Triton X-100 (proteomics grade, VWR: 97063-864) diluted in 1X phosphate-buffered saline (PBS ...
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bioRxiv - Genomics 2021Quote: Tissue sections were prepared from formalin-fixed paraffin embedded tissue blocks and cut to 4 micrometers serial sections and mounted on Superfrost Plus (VWR). The procedure for multiplex immunohistochemistry (mIHC ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: Tissue specimens were transported directly from surgical theatre to the laboratory in ice-cold sterile Phosphate Buffered Saline (VWR, UK). LVs were identified and isolated along with a small amount of surrounding tissue under a dissection microscope within a sterile laminar flow cabinet (Fig 1 A-C) ...
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bioRxiv - Bioengineering 2022Quote: ... channels were gently removed from the adhesive carrier sheet using fine-tipped tweezers and sonicated in ethanol (VWR, PA, USA) for 5 min ...
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bioRxiv - Immunology 2020Quote: Feces from donor mice were diluted (30-50 mg - 1:10 w/vol) and homogenized in reduced sterile Ringer solution (VWR) containing L-Cysteine (0.5 g/L - Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.4 µm TC Plate Insert with a PC Membrane 6 well plate (thinserts) was purchased from VWR (Radnor Pennsylvania, USA). PD-L1 ...
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bioRxiv - Biochemistry 2022Quote: ... The handling of this liquid and the titration of the metals from a 96-well deep block (VWR, #82051-260), was performed by a EvoWare v2.7 scripted Tecan Freedom Evo 100 base equipped with a Tecan fixed-tip liquid handling arm ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was prepared from excised root tips (tip growth gained after seedling transfer) by using the peqGOLD Plant RNA Kit (VWR). One biological replicate represents 40-60 pooled root tips ...
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bioRxiv - Cell Biology 2020Quote: ... dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We removed nearly all dissection/fixation solution from the sample and picked up the coverslip with a HistoBond microscope slide (VWR). Samples were then frozen on dry ice ...
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bioRxiv - Neuroscience 2023Quote: Hypothalamic progenitors differentiated from the HUES9-POMC-GFP cell line were re-plated onto 24 mm round glass coverslip (VWR) that has been pre-treated with 5 M hydrochloric acid and double coated with both 0.01% poly-L-ornithine hydrobromide (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Analytical grade ammonia solution (28%, GPR RECTAPUR®) and acetic acid glacial (AnalaR® NORMAPUR®) were obtained from VWR Chemicals.
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bioRxiv - Molecular Biology 2022Quote: ... 0.23 µl fluorescein (100X, prepared according to “Fluorescein preparation for naica® system” from Stilla Technologies) (0681-100G, VWR Chemicals), 1 µl of diluted RNA sample (or a single cell) ...
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bioRxiv - Cancer Biology 2022Quote: Peripheral blood mononuclear cells (PBMC) from 5 healthy young adult donors (mean age 28) were isolated by Ficoll-Paque (VWR) density centrifugation and stained with fixable viability dye efluor506 (eBioscience ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Formic acid (FA), 2-proponol, acetonitrile (ACN), and methanol (all HiPerSolv CHROMANORM, HPLC grade, BDH Prolabo) were purchased from VWR International (Helsinki ...
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bioRxiv - Pathology 2024Quote: Six μm sections of formalin-fixed paraffin-embedded (FFPE) tissue from the dorsolateral prefrontal cortex (Brodmann Area 9) were de-paraffinized with CitriSolv (Xylene substitute, VWR) for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed by adding 100 µl 8% PFA for 20 min at RT (final concentration of PFA is 4%; PFA from VWR). All media was then gently removed and cells were washed 3 times for 5 minutes with 150 µl PBS ...
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bioRxiv - Genetics 2023Quote: ... Both media were supplemented with 10% fetal bovine serum (FBS, Nucleus Biologics, LLC) and 1% penicillin/streptomycin (from VWR, LLC). All cells were cultured in an incubator with 5% CO2 at 37°C.
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bioRxiv - Neuroscience 2023Quote: ... von Frey filaments were obtained from Ugo Basile and were calibrated in the lab using a weigh scale (VWR, 314AC) to 0.01 grams ...
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bioRxiv - Developmental Biology 2022Quote: RNA from embryonic kidneys was extracted by the use of the peqGold Total RNA-Kit (VWR International GmbH, Darmstadt, Germany) according to the manufacturer’s instructions ...
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Single-cell assessment of trophoblast stem cell-based organoids as human placenta-modeling platformsbioRxiv - Developmental Biology 2022Quote: ... The supernatant from the second and third digestions were then pooled and strained through a 100 μm cell strainer (VWR). Pooled and digested villous suspensions were then centrifuged at 4°C at a speed of 1200 rpm for 10 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... Effluent was collected from a steam-sterilized sample valve in the bottom of the bioreactor into sterilized plastic Nalgene bottles (VWR) equipped with a gas exhaust filter ...
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bioRxiv - Neuroscience 2022Quote: ... 40 ¼m coronal sections were cryostat cut through the rostrocaudal extent from cortex to the brainstem and slide-mounted (Superfrost Plus, VWR). Mounted sections were then stored at −80 °C for subsequent immunohistochemistry (IHC ...
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bioRxiv - Plant Biology 2022Quote: ... were punched from the basal region using a sterile biopsy punch (pfm medical) and transferred to a 96-well plate (VWR). Bioluminescence was measured in a FLUOstar Omega plate reader (BMG Labtech).
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Label-free digital holotomography reveals ibuprofen-induced morphological changes to red blood cellsbioRxiv - Cell Biology 2022Quote: Whole blood was freshly obtained with the consent of a healthy donor from a finger prick with safety lancets (VWR). Sickle cell trait (SCT ...
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bioRxiv - Biophysics 2022Quote: Muscle tissue culture molds were fabricated from polymethyl methacrylate (PMMA) and glued onto a microscopy cover glass (VWR, Radnor, USA) as previously described[22] ...
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bioRxiv - Genomics 2024Quote: ... Enzymes were purchased from New England Biolabs (NEBNext Companion Module #E7180) and Omega Bio-Tek Mag-bind TotalPure NGS magnetic beads were from VWR International ...
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bioRxiv - Microbiology 2024Quote: ... pOXA-48-carrying and pOXA-48ΔlysR-carrying KPN15 and CF13 were performed in the presence of the Iron Chelator 2,2-Bipyridyl from VWR (ref. 30569.09). Concentrations of 2,2-Bipyridyl ranging from 0.016 mM to 0.5 mM were tested ...
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bioRxiv - Biochemistry 2024Quote: ... Axygen 12-Channel Through (cat#732-1390) and reagent reservoirs (cat#89094-666 and cat#89094-668) were obtained from VWR International GmbH (Bruchsal ...
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bioRxiv - Biochemistry 2024Quote: ... was supplied from an LA755 Elga purification system (Elga Lab water, High Wycombe, USA) and isopropyl alcohol (IPA) and ethanol were obtained from VWR International (Radnor ...
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bioRxiv - Bioengineering 2024Quote: Pre-cleaned conditioned media (700 x g for 5 min and 2000 x g for 10 min) from HeLa-WT and HeLa-CD81-Snorkel-tag overexpressing cells was sterile filtered using syringe (VWR) with cellulose acetate membrane filters (0.22 µm pore size ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Oxford Nanopore Rapid Barcoding Kit and R10.4 flow cell were purchased from VWR (USA, #SQK-RBK114.96 and #FLO-MIN114). For the short-reads ...