Labshake search
Citations for Zymo Research :
451 - 500 of 6307 citations for PCR Product Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2019Quote: ... The reaction was incubated 10 minutes at 55°C followed by purification with DNA Clean & Concentrator-5 kit (Zymo Research) and elution in 21 µL of water ...
-
bioRxiv - Cell Biology 2021Quote: ... Total RNA from all samples was isolated by column purification (Direct-zol RNA Microprep kit, #R2061, Zymo Research, Irvine, CA) and converted to cDNA (qScript ...
-
bioRxiv - Genetics 2020Quote: ... Genomic DNA was purified from WT and Chr4-cl KO ES cells using the Quick-gDNA purification kit (Zymo Research) and bisulfite-converted with the NEXTflex™ Bisulfite-Seq Kit (Bio Scientific ...
-
bioRxiv - Zoology 2020Quote: ... RNA purification and DNAse treatment were conducted following the Direct-zolTM RNA MiniPrep Kit protocol (Zymo Research, Irvine, CA, USA) with slight modifications ...
-
bioRxiv - Genomics 2021Quote: ... RNA was extracted from inactivated swab samples using the Quick DNA/RNA Viral Magbead kit from step 2 of the DNA/RNA purification protocol (Zymo) (files.zymoresearch.com/protocols/_r2140_r2141_quick-dna-rna_viral_magbead.pdf).
-
bioRxiv - Cancer Biology 2023Quote: Total RNA samples isolated from PLC/PRF/5 cells with or without TARBP1 depletion were first subjected to small RNA purification using the Zymo Research RNA Clean & Concentrator-25 kit (Zymo). The isolated small RNAs were heated at 75°C for 2 min to denature tRNA and then incubated with ALKB-D135S protein in buffer containing 300 mM KCl ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA was isolated using phenol-chloroform extraction followed by purification with Zymo Direct-zol RNA Miniprep Kit (Zymo Research R2052). Genomic DNA removal and cDNA synthesis were performed with iScript gDNA clear cDNA synthesis kit (Bio-Rad 1725035 ...
-
bioRxiv - Biochemistry 2024Quote: Total RNA was extracted using hot acid phenol method followed by purification using Direct-zol RNA Miniprep Kit (Zymo Research). The RNA was treated with DNaseI ...
-
bioRxiv - Cancer Biology 2023Quote: ... These spike-in samples then undergone bisulfite conversion and purification by EZ DNA Methylation-Gold Kit (Zymo Research, D5006, USA). DNA library was prepared from bisulfite-converted DNA samples using xGen Methyl-Seq DNA Library Prep Kit (Integrated DNA Technologies ...
-
bioRxiv - Bioengineering 2019Quote: ... These PCRs were purified using the DNA Clean & Concentrator kit (Zymo Research) according to manufacturer’ instructions ...
-
bioRxiv - Immunology 2021Quote: ... PCR amplicons were purified using the DNA Clean & Concentration kit (Zymo Research), and dsRNAs were synthesized using the MEGAscript RNAi kit (Life Technologies) ...
-
bioRxiv - Genetics 2023Quote: ... treated with the Zymo PCR Inhibitor Removal kit (Zymo Research, Irvine CA) to remove melanin ...
-
bioRxiv - Genetics 2024Quote: ... PCR amplicons were purified using DNA Clean & Concentrator-5 kit (Zymo Research) following the manufacturer’s recommendations ...
-
bioRxiv - Genomics 2024Quote: ... followed by cleanup with the OneStep PCR Inhibitor Removal Kit (Zymo Research). qPCR quantification was carried out in duplicate for each replicate with primers PrymF2239 (5’-CACATCCGATCGTGTCTGC-3’ ...
-
bioRxiv - Biochemistry 2021Quote: ... Cloning product was first subcloned in NEB-5α strains and then purified using ZymoPURE plasmid miniprep kits (Zymo Research). The sequence of the expression construct was verified with Sanger sequencing by Sequetech ...
-
bioRxiv - Molecular Biology 2021Quote: ... The DNA products were separated on a 3% agarose gel and then extracted using gel extraction kit (ZYMO research). In vitro transcription with T7 RNA polymerase and in vitro Dicer cleavage assays were performed as previously described (Sheng et al ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Products were combined at equimolar concentrations and purified using a Zymo DNA Clean and Concentrator kit (Zymo Research D4014) to create a single multiplexed library for sequencing ...
-
bioRxiv - Genomics 2019Quote: ... DNA was purified using column purification (Zymo #D4004) and size-selected by taking the unbound fraction of 0.55X AMPure XP beads (Beckman Coultier #A63880 ...
-
bioRxiv - Genomics 2022Quote: ... Decrosslinked DNA was purified by column purification (Zymo) and used for quantitative reverse transcription PCR (qPCR ...
-
bioRxiv - Genetics 2023Quote: ... and cleaned by column-based purification (Zymo Research). Concentrations and RNA quality were determined using RNA ScreenTapes and a 4200 Tapestation System (Agilent Technologies) ...
-
bioRxiv - Microbiology 2020Quote: ... PCR reactions yielding single amplicons as judged by agarose gel electrophoresis were purified using a PCR clean up kit (Zymo Research) and sent for DNA sequencing ...
-
bioRxiv - Cell Biology 2020Quote: ... and the RNA was extracted using the Direct-zol™ RNA purification kit following the manufacturer’s protocol for DNase I digestion (Zymo Research). cDNA was synthesized with Maxima Reverse Transcriptase (Thermo Scientific™ ...
-
bioRxiv - Genetics 2021Quote: ... We extracted and isolated RNA by phase separation using standard protocols followed by purification using the Direct-zol RNA microprep kit (Zymo R2060) with an on-column DNAse step per manufacturer directions ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were resolved on 2% agarose gels followed by extraction and purification of candidate DNA amplicons using Zymoclean Gel DNA Recovery Kits (Zymo Research). Purified PCR products were cloned using the TOPO TA cloning kit (ThermoFisher Scientific) ...
-
bioRxiv - Developmental Biology 2021Quote: ... the four guide RNA templates were pooled together and concentrated through purification by a DNA Clean and Concentrator kit (Zymo Research).
-
bioRxiv - Molecular Biology 2020Quote: ... The resulting product was then treated with Turbo DNase and the remaining RNA was purified with the Zymo RCC-25 column purification-25 kit (Zymo Research). The A/B RNA standards were quantified using Qubit RNA HS Assay on Qubit 2.0 Fluorometer (Life Technologies ...
-
bioRxiv - Genomics 2020Quote: ... 4-S-UTP containing RNA was eluted in 100mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research) with in-column DNAse reaction to eliminate traces of genomic DNA ...
-
bioRxiv - Microbiology 2020Quote: Dim and lit cells were sorted into 500 µl TriZol and RNA was extracted with DirectZol RNA purification kit (Zymo Research) with the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... The linear A/B standards were finally treated with Proteinase K and further purified with the Zymo ChIP DCC-25 purification kit (Zymo Research). The final A/B standards were quantified using Qubit dsDNA HS Assay on Qubit 2.0 Fluorometer (Life Technologies ...
-
bioRxiv - Genetics 2022Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using a YeaStar genomic DNA purification kit (Zymo Research). Equal quantities of DNA from four spontaneous canavanine-resistant clones derived from strain 2299 were combined ...
-
bioRxiv - Cell Biology 2022Quote: ... The samples were then placed on a magnet and the immunoprecipitated DNA samples in the supernatant along with the input samples were purified using ChIP Purification Kit (Zymo Research), according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... DNA was purified from proteinase K treated samples using a DNA purification kit following the manufacturer instructions (Zymo Research, CA, USA) and was subsequently analyzed either by running a 2% low melting agarose (APEX ...
-
bioRxiv - Molecular Biology 2019Quote: ... RNA was released by proteinase K treatment in urea and recovered by phenol chloroform extraction and column purification (RNA Clean-Up kit; Zymo Research). Input samples were dephosphorylated and 3’-linker ligated to a custom oligonucleotide primer and all samples (IPs and SMInputs ...
-
bioRxiv - Genomics 2020Quote: ... 4-SUTP containing RNA was eluted in 100 mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research) with in-column DNAseq reaction to eliminate traces of genomic DNA ...
-
bioRxiv - Cell Biology 2020Quote: Total RNA from cultured NSC-34 cells or primary motor neurons was isolated using DirectZole RNA Purification Kit (Zymo Research, R2052), and reverse transcribed using qScript cDNA Synthesis Kit (Quanta Biosciences ...
-
bioRxiv - Cell Biology 2021Quote: ... Mixture was incubated at 37 ℃ for 30 min followed by purification with a Zymo DNA Clean and Concentrator-5 Kit (Zymo, DP4033). Transposed DNA was eluted in 24 μl elution buffer and amplified by PCR reaction (10 μl 5 X TAB ...
-
bioRxiv - Genomics 2021Quote: ... The mixture was incubated at 37°C for 30 minutes and subsequently purified using the ChIP DNA purification kit (Zymo Research) as described by the manufacturer ...
-
bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated for 45 min (NMIA) or 5 min (1M7) at 37 or 60°C following purification using Direct-zol RNA MiniPrep Kit (Zymo Research).
-
bioRxiv - Genomics 2023Quote: Total RNA was isolated from the received fungal pellet using conventional TRIzol method followed by column purification using Quick RNA Plant MiniPrep Kit (Zymo Research). The qualities and quantities of the isolated RNA were checked on NanoDrop followed by Agilent TapeStation using High Sensitivity RNA ScreenTape.
-
bioRxiv - Bioengineering 2022Quote: cDNA samples were again purified via column purification using a Zymo Research DNA Clean and Concentrator-25 kit (Zymo, Irvine, CA). Select 200-300 bp regions were then amplified using specific primers designed against various hotspot regions found in the patient-sample derived consensus sequence (Supplementary Table 5) ...
-
bioRxiv - Cell Biology 2024Quote: ... Assembled plasmids (termed pTREP-ID vector hereafter) were then purified using a DNA Clean and Concentrator purification kit (Zymo, no. D4014) and transformed into One Shot ccdB Survival 2 T1R resistant competent cells (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from cultured cells or myocardial tissue of mice euthanized at the timepoints reported above using the Direct-zol RNA purification Kit (ZYMO Research) following the manufacturer’s protocol.
-
bioRxiv - Biochemistry 2024Quote: ... which were removed from the mutational libraries by gel purification using a Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Purified products were again transformed into either NEB 10-beta (New England Biolabs ...
-
bioRxiv - Microbiology 2024Quote: ... using bead-beating for 30 min at RT followed by purification using Direct-zol RNA Miniprep kit (Zymo Research; Tustin, CA). Eluted RNA was analyzed in a Nanodrop Lite (Thermo Scientific) ...
-
bioRxiv - Microbiology 2024Quote: ... S4) and cells were collected by filtration for RNA purification using the RNA Clean and Concentrator kit (Zymo Research, Irvine CA). rRNA depletion was accomplished with the Ribo-Zero rRNA Removal Kit (Epicentre ...
-
bioRxiv - Genetics 2019Quote: ... These products were gel purified (Zymo Research) and used as templates for the second PCR with a primer containing the MmeI restriction site ...
-
bioRxiv - Biophysics 2021Quote: ... Final products were gel purified (Zymo Research), quantified by Qubit (ThermoFisher) ...
-
bioRxiv - Genomics 2022Quote: ... The product was column purified (Zymo D4014) and PCR amplified for 2 cycles using the NEBNext master mix (NEB M0544L ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Amplified products were column purified (Zymo Research), digested with EcoR1 and BamH1 ...
-
bioRxiv - Synthetic Biology 2019Quote: ... which was purified and size-selected for 145 bp products using the Select-a-Size Clean and Concentrator kit (Zymo). The library was quantified with the Qubit dsDNA HS Assay Kit (Invitrogen ...