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Citations for Zymo Research :
551 - 600 of 6307 citations for PCR Product Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and DNA was purified by spin column purification (Zymo, Inc.). DNA was eluted in 20 uL of Tris-HCl and stored at -20°C.
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bioRxiv - Synthetic Biology 2023Quote: ... followed by purification (DNA Clean & Concentrator, Zymo Research cat #D4004). Libraries were assembled using the HiFi DNA Assembly kit (NEB ...
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bioRxiv - Microbiology 2023Quote: ... before purification using an RNA Clean & Concentrator column (Zymo Research). 20 pmoles of dephosphorylated RNA was combined with 2 μl 10 x T4 Polynucleotide Kinase buffer (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... and linearized plasmid was isolated by gel purification (Zymo Research). The CDS fragments for cloning were amplified by conventional PCR from wild-type genomic DNA using oligos listed in Table S4 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by column purification (Zymo RNA Clean and Concentrator Column) according to the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by purification with RNA Clean & Concentrator-5 (Zymo research). All the subsequent steps were performed according to Illumina’s TruSeq Ribo-Profile protocol with minor modifications as described in [73] ...
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bioRxiv - Cancer Biology 2024Quote: ... ZR Plasmid Miniprep Classic plasmid purification (ZYMO Research, Lucerna-Chem), and Sanger DNA sequencing (Microsynth ...
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bioRxiv - Cell Biology 2024Quote: ... and all reactions with the appropriate molecular weight product were combined and cleaned using the DNA Clean and Concentrator 5 kit (Zymo Research D4013). 10 µg LentiCRISPRv2-opti (Addgene #163126 ...
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bioRxiv - Molecular Biology 2022Quote: ... Qiagen DNeasy Blood and Tissue Kit and post-extraction inhibitor removal with the Zymo Research OneStep™ PCR Inhibitor Removal Kit (QBT-ZYMO), 4 ...
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In vitro impact of fluconazole on oral microbial communities, bacterial growth and biofilm formationbioRxiv - Microbiology 2023Quote: ... and quantified by real-time PCR using Zymo DNA quantification kits for bacteria/fungi (Femto Bacterial and fungal DNA Quantification kit, Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: Total RNA was obtained from choroidal EC cultures and frozen RPE cells using an RNA purification kit (Direct-zol RNA MiniPrep; Zymo Research, Irvine, CA, USA), as per the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Three DNA libraries from the same DNA extraction were prepared with further purification and concentration using the Genomic DNA Clean and Concentrator kit (Zymo Research, Irvine, CA, USA). The DNA was then selected for high molecular weight DNA using the PacBio Short Read Eliminator kit (PacBio ...
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bioRxiv - Zoology 2024Quote: ... These amplified products were then purified as above and restriction enzyme digested CCHa1R and CCHa2R sequences were ligated into pcDNA3.1+ and pBudCE4.1 mammalian expression vectors followed by bacterial transformation and plasmid DNA purification to obtain a large quantity of receptor constructs using ZymoPURE II Plasmid Midiprep Kit (Zymo Research, Tustin, CA, USA) following the manufacturer recommendation ...
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bioRxiv - Microbiology 2020Quote: ... Extractions were then purified with the OneStep PCR Inhibitor Removal Kit (Zymo Research, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... The PCR amplicons were purified by Zymoclean™ Gel DNA Recovery Kit (ZYMO RESEARCH, D4008) and phosphorylated at the 5’ termini by T4 Polynucleotide Kinase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... #12 DNA were purified using the OneStep PCR Inhibitor Removal Kit (Zymo Research, CA, USA). Then ...
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bioRxiv - Microbiology 2022Quote: ... Melanin was removed from genomic DNA using the OneStepTM PCR Inhibitor Removal kit (Zymo Research). Genomic DNA was measured by Nanodrop and diluted to ∼20ng/µl ...
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bioRxiv - Microbiology 2023Quote: ... Inhibitors were removed from the DNA extracts using the OneStep PCR Inhibitor Removal Kit (Zymo) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... PCR reactions were purified from gel using the Zymoclean Gel DNA Recovery Kit (Zymo Research). Seventy nanograms of each purified fragment were used for Golden Braid domestication in pUPD2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by RNA isolation according to the TRIzol product instructions or using a Direct-zol RNA MiniPrep Plus kit (ZYMO Research Corp, 2072). DNA contamination was removed from the TRIzol-isolated RNA via enzymatic digestion with 10 U of Turbo DNase (Ambion ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were separated by electrophoresis on a 1% agarose gel and purified using the Zymoclean Gel DNA recovery Kit (Zymo Research, Irvine, CA). The purified DNA fragments were then submitted for Sanger sequencing (GENEWIZ ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μL of each reaction product were pooled and purified using the Zymo DNA Clean and Concentrator kit (Zymo Research, Irvine, CA). Sequencing was performed at the Chan Zuckerberg BioHub facility on an Illumina NextSeq 550 platform in High Output mode.
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bioRxiv - Cancer Biology 2022Quote: ... The outer PCR products amplified using primer #1 and #2 were purified and concentrated using the DNA Clean and Concentrator 5 kit (Zymo Research, Orange, CA) and eluted in 20ul of TE buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA purification was performed using RNA Clean and Concentrator (Zymo Research) with the optional DNase I treatment ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA was purified using a Zymo DNA purification column (Zymo Research).
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bioRxiv - Microbiology 2021Quote: ... After purification with DNA Clean & Concentrator-25 (Zymo Research Corporation, D4034), the purified PCR product was respectively inserted into the three sgRNAeBAR-expressing backbones constructed above through the Golden Gate cloning strategy (47) ...
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bioRxiv - Genetics 2021Quote: ... we purified samples using Select-A-Size purification columns (Zymo D4080) with a cutoff of 150bp to remove adapter dimers to allow for efficient sequencing on patterned flow cells ...
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bioRxiv - Genomics 2023Quote: ... Purification was then done with DNA Clean & Concentrator-5 (ZYMO D4013) according to the manufacturer’s guidelines ...
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bioRxiv - Biophysics 2023Quote: ... then purified by column purification (RNA Clean & Concentrator, Zymo Research #R1017). After denaturing the RNA for 3 minutes at 90°C followed by 10 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The parasites were washed twice with PBS prior to RNA extraction and purification using the Quick-RNATM MiniPrep kit (Zymo Research, Irvine, CA, United States) according to manufacturer specifications ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR digested fragments were purified from agarose gel by Zymoclean Gel DNA Recovery Kit (Zymo Research). Heavy and light chain full IgG p3BNC expression plasmids were divided to three parts for PCR amplification ...
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bioRxiv - Microbiology 2021Quote: ... Eluted sample was cleaned-up using the OneStep PCR Inhibitor Removal Kit (Zymo Research, Irvine, California), and DNA was quantified using a Quantus Fluorometer (Promega Corporation ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA fragment was generated by PCR amplification and purified by DNA Clean & Concentrator Kit (Zymo Research). gRNA plasmids were constructed according to the ‘gRNA-tRNA Array Assembly’ methods described in the Multiplex MoClo Toolkit29 ...
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bioRxiv - Cancer Biology 2023Quote: ... and further cleaned up by a OneStep PCR Inhibitor Removal Kit (D6030; Zymo Research, Irvine, CA) before PCR amplification ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA for quantitative RT-PCR assays was isolated using the Quick-RNA Microprep kit (Zymo) with on-column DNase treatment per the manufacturer’s protocol.
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bioRxiv - Genetics 2019Quote: ... This second product was purified by silica column (Zymo Research) and digested with MmeI (NEB) ...
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bioRxiv - Genomics 2021Quote: ... 200 µL of L-Desulphonation buffer (Zymo Research, product #5030) was added to the columns for 15 minutes ...
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bioRxiv - Genomics 2021Quote: ... 200 µL of L-Desulphonation buffer (Zymo Research, product #5030) was added to the columns for 20 minutes ...
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bioRxiv - Systems Biology 2024Quote: ... The products were gel purified (Zymo Gel Clean and Concentrator), then quantified using Qubit High- Sensitivity dsDNA fluorimeter kit before submission for sequencing on Illumina HighSeq runs at NovoGene (S13).
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bioRxiv - Molecular Biology 2021Quote: ... followed by purification over the RNA Clean & Concentrator-25 columns (Zymo Research) and elution in DEPC H2O ...
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bioRxiv - Immunology 2019Quote: ... Purification of transposed DNA was completed with DNA Clean and Concentrator (Zymo) and fragments were barcoded with ATAC-seq indices36 ...
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bioRxiv - Bioengineering 2021Quote: ... we extracted total RNA from HEK293FT cells using Direct-zol purification (Zymo) and reverse transcribed 1 ug of RNA into cDNA using Revert-Aid (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by purification over the RNA Clean & Concentrator-25 columns (Zymo Research) and elution in DEPC H2O ...
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bioRxiv - Genetics 2022Quote: ... A Zymo DNA Clean & Concentrator purification step (Zymo Research, Irvine, CA, USA) was performed to remove the excess salts ...
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bioRxiv - Synthetic Biology 2020Quote: ... desalted and recovered by isopropanol precipitation or by column purification (Zymo Research). The pellet was re-suspended in water and column purified to remove any residual ATP from the previous ligation step ...
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bioRxiv - Immunology 2021Quote: ... Purification and desulphonation of converted DNA was performed with magnetic beads (Zymo) on a Bravo Workstation (Agilent) ...
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bioRxiv - Microbiology 2022Quote: ... followed by purification using Direct-zol RNA Miniprep Plus (Zymo Research #R2072) and reverse transcription using iScript cDNA synthesis kit (Bio-Rad #1708891).
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bioRxiv - Immunology 2023Quote: ... Purification of transposed DNA was completed with DNA Clean and Concentrator (Zymo) and fragments were barcoded with ATAC-seq indices ...
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bioRxiv - Molecular Biology 2023Quote: ... for 30min at 30°C followed by column purification (Zymo research, R1016). The following reverse transcription (RT ...
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bioRxiv - Synthetic Biology 2023Quote: ... the shuttle plasmid was purified using column purification (Zymo Research CN# D4013) according to manufacturer protocols.