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Citations for Zymo Research :
301 - 350 of 437 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Most of the supernatant was removed and 750 μl of ZymoBIOMICS Lysis Solution and 19 μl of proteinase K (D3001-2-20/D3001-2-5, Zymo Research) were added to the remaining 200 μl of the samples and incubated for 30 min at +55 °C ...
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bioRxiv - Neuroscience 2022Quote: ... the resulting solution was incubated with 3 volumes of TRI-reagent at room temperature for 2 hours (R2050-1-50; Zymo Research). The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080 ...
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bioRxiv - Molecular Biology 2024Quote: Liver samples (∼15mg) collected at euthanasia were placed in 0.75mL of 1X DNA/RNA Shield™ solution (Zymo Research, Irvine, CA), shipped to Zymo Research ...
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bioRxiv - Microbiology 2023Quote: Fecal pellets were collected for individual mouse in each group at the same day and stored in DNA/RNA shield solution (Zymo Reasearch) at −20°C until further processing ...
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bioRxiv - Biochemistry 2024Quote: ... Frozen cell pellets were thawed and suspended in 200 µl of solution P1 and treated with 7 μL Zymolyase (0.2 units/μL; Zymo Research #E1005) at 37°C for 1.5 hr ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The samples were subjected to mechanical grinding at 2700 rpm for 20 minutes in a ZR BashingBead Lysis Tube 2,0 mm with 750 µL of lysis solution (Zymo Research, Germany) on a Vortex Genie 2 (Scientific Industries) ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was isolated from whole cells for qRT-PCR using Quick RNA miniprep plus kit from Zymo Research following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... the probe library was generated with PCR amplification and recovery by DNA Cleanup kit (D4014, Zymo research, U.S.A.). Then Tn5-FISH probes were amplified by a second PCR with fluorescence-tagged primers ...
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bioRxiv - Cancer Biology 2020Quote: Approximately 1.4kb of genomic fragment spanning SALL4 5’ UTR - exon 1 - intron 1 was PCR amplified (Zymo Research) and cloned into the pGEM-T Easy vector ...
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bioRxiv - Biochemistry 2020Quote: ... Products of the first-round PCR were pooled and purified by a DNA clean and concentrator kit (Zymo) and diluted to 2 ng/μL ...
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bioRxiv - Molecular Biology 2020Quote: ... and the Gaac.1826 target locus was PCR amplified and purified using DNA Clean & Concentrator™ (Zymo Research) and Sanger sequencing was performed (Retrogen) ...
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bioRxiv - Systems Biology 2020Quote: ... 2 μL of PCR products from each well were pooled and purified using the DNA Clean & Concentrator (Zymo) and eluted in water ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA PCR was conducted after extraction of genomic DNA using a Zymobead genomic DNA kit (Zymo Research). Primer pair amplifying hGAA and His-hGAA (GAA gD F1 ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR pool cleanup was done by column purification with a DNA Clean and Concentrator kit (Zymo Research). The purified samples were sent for EZ-amplicon sequencing using Illumina-based technology (2×250 bp reads ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from whole cells for qRT-PCR using Quick RNA miniprep plus kit from Zymo Research following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Clones of interest were identified by PCR on genomic DNA harvested with the Quick-DNA Miniprep Kit (Zymo) from expanded cell lines using primers flanking exon 2 followed by Sanger sequencing of amplicons by Genewiz ...
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bioRxiv - Genomics 2022Quote: ... the amplified PCR products were purified using the Zymo dna clean and concentrator kit (Zymo Research, Cat. D4014) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA purification after the PCR and gel electrophoresis was performed using the DNA Clean & Concentrator Kit (Zymo Research) and Zymoclean Gel DNA Recovery kit (Zymo Research) ...
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bioRxiv - Cell Biology 2024Quote: ... After purifying both PCR products using a 1% agarose gel and a gel purification kit (Zymo, no. D4007), the two fragments were assembled using a Gibson assembly mix (NEB ...
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bioRxiv - Microbiology 2024Quote: ... the PCR master mix has been treated with 0.02% (v/v) of DNAse I 1 U/mL (Zymo) at room temperature for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR product was then cleaned up using a DNA Clean and Concentrator kit-5 (Zymo Research Cat. #D4003), and eluted in 15μL H2O ...
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bioRxiv - Biochemistry 2024Quote: ... These PCR products were then purified using a Zymo DNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA). Finally ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was prepared by using ISOGEN (Nippon Gene, Japan) and PCR Inhibitor Removal Kit (Zymo Research, USA). The RNA was reverse transcribed to cDNA by using Superscript III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... linear PCR products were purified from agarose gel (1%) with a Zymoclean-Gel DNA Recovery kit (Zymo Research) and circularized with DNA Ligation Kit ‘Mighty Mix’ (Takara) ...
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bioRxiv - Genomics 2023Quote: ... PCR was performed for 13 cycles and libraries were purified with column-based DNA purification (Zymo Research, D4014) followed by 0.8x Ampure bead cleanup ...
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bioRxiv - Biophysics 2023Quote: ... The PCR reactions were ethanol precipitated before being purified using a DNA Clean and Concentrator Kit (Zymo Research). The concentration of the DNA was determined by UV Vis ...
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bioRxiv - Systems Biology 2023Quote: ... 2 μL of PCR products from each well were pooled and purified using the DNA Clean & Concentrator (Zymo) and eluted in water ...
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bioRxiv - Bioengineering 2023Quote: ... PCR amplicons were purified directly or gel purified using Zymoclean Gel DNA Recovery Kit (Zymo Research Cat. #D4007) and then Sanger sequenced ...
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bioRxiv - Genomics 2024Quote: The PCR reaction contained 2 µl of bisulfite treated DNA sample (EZ DNA Methylation-Gold kit, Zymo Research), 2.5 µl of buffer + 0.05 µl of Taq Polymerase (PCRBIO Classic Taq ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR product was run on an agarose gel and purified by gel DNA recovery kit (Zymo Research), followed by cloning into pGEM-T vector by pGEM-T easy vector system (Promega) ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR fragments were purified using DNA Clean & Concentrator-5 kits as per the manufacturer (Zymo Research, Irvine, CA), eluted in 20 μL or RNase-free water ...
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bioRxiv - Neuroscience 2024Quote: ... The PCR product was then purified using the Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA, USA) and subsequently cloned into the pGL3-promoter vector downstream of the firefly luciferase reporter gene by Gibson Assembly (New England Biolabs ...
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bioRxiv - Genetics 2024Quote: ... PCR reactions of the same samples were pooled and concentrated using the DNA Clean & Concentrator Kit (Zymo Research). Lastly ...
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bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant was transferred to a new 15 mL falcon tube containing 3.25 mL of ammonium bisulfite solution (Zymo Research, product #5030) and heated to 98°C for 10 minutes ...
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bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant was transferred to 15 mL falcon tube containing 3.25 mL of ammonium bisulfite solution (Zymo Research, product #5030) and shaken in a thermomixer at 98°C for 10 minutes (15s on/30s off) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the total RNA was extracted from the clear aqueous phase of the solution using Direct-zol™ RNA MiniPrep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... were pretreated with a solution of β-mercaptoethanol plus 60 mM EDTA and then incubated with 6 U/mL zymolyase (Zymo Research) in Buffer I to remove the cell wall ...
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bioRxiv - Bioengineering 2020Quote: Total DNA was extracted from fecal and mucosal samples preserved in DNA/RNA Shield (DRS) solution (R1100-250, Zymo Research, Irvine, CA, USA) or fresh using ZymoBIOMICS DNA Miniprep Kit (D4300 ...
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bioRxiv - Genetics 2024Quote: ... A sample of sporulation culture with approximately 108 cells/mL was mixed 9:1 with a 5U/µL solution of Zymolyase (Zymo Research, cat. E1004) to a final zymolyase concentration of 0.5U/µL and incubated at 30°C for 5 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR reactions were performed according to manufacturer’s instructions and cleaned up using clean and concentrate kits (D4004-Zymo Research). 600 nM of each amplified transcriptional unit library was transformed using the lithium acetate protocol described above ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were run on 1% agarose gel and purified using the Zymoclean gel DNA recovery kit (Zymo Research).
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR reactions were then collected and concentrated using the Zymo DNA clean concentrator kit (Zymo research®, D4032). The concentration was performed according to the manufacturer protocol and the libraries were eluted in 2,800 μl of RNase/DNase free water ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR and digestion products were recovered from agarose gels following electrophoresis using the Zymogen Gel Recovery Kit (Zymo Research). DNA assembly was performed using NEBuilder 2x Master Mix (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... and 50 uL of the pooled PCR product was purified with the DNA Clean and Concentrator kit (Zymo Research). The final BarSeq library was eluted in 40 uL water ...
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bioRxiv - Biochemistry 2020Quote: ... The entire PCR reaction is run on 1% agarose gel and the DNA at 230bp was purified (Zymo Research). See Supplemental Figure S2.
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bioRxiv - Immunology 2021Quote: ... Cxcl1 promoter regions #1 and #2 were PCR amplified from sodium bisulfite converted gDNA using ZymoTaq Polymerase (Zymo Research) and purified by gel extraction using the QIAquick Gel Extraction Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... and 50 μL of the pooled PCR product was purified with the DNA Clean and Concentrator kit (Zymo Research). The final BarSeq library was eluted in 40 μL water ...
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bioRxiv - Microbiology 2020Quote: ... and 50 ul of the pooled PCR product was purified with the DNA Clean and Concentrator kit (Zymo Research). The final BarSeq library was eluted in 40 ul water ...
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR products were run on agarose gels and purified by Zymoclean Gel DNA Recovery Kit (Zymo Research, CA) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were isolated and concentrated using the Zymoclean Large Fragment DNS Recovery Kit (Zymo Research, Irvine, CA, USA) and DNA Clean & Concentrator-5 (Zymo Research ...