Labshake search
Citations for Zymo Research :
251 - 300 of 437 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR products were purified with ZYMO DNA Clean and Concentrator column (ZYMO Research Cat# D4004). The concentration was measured by Qubit dsDNA HS kits with appropriate dilutions (Invitrogen Q32851) ...
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bioRxiv - Synthetic Biology 2022Quote: ... and PCR products were purified using the DNA Clean and Concentrator Kit (Zymo Research, Irvine, CA). Plasmids generated in this work are listed in Table 1.
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bioRxiv - Synthetic Biology 2024Quote: ... DNA fragment was generated by PCR amplification and purified by DNA Clean & Concentrator Kit (Zymo Research). gRNA plasmids were constructed according to the ‘gRNA-tRNA Array Assembly’ methods described in the Multiplex MoClo Toolkit29 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR product was purified using the DNA Clean and Concentrator-5 kit (Zymo Research, #D4004) and transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR products were pooled and column purified using a DNA Clean and Concentrate Kit (Zymo Research) and quantified using a Qubit 3.0 fluorometer.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The PCR products obtained were purified using DNA clean and ConcentratorTM-25 (Zymo Research, CA, USA). The purified products were further treated with T4 DNA polymerase ...
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bioRxiv - Genetics 2023Quote: ... and PCR products were cleaned up using the DNA Clean & Concentrator-5 kit (Zymo Research #D4014). We later quantified the concentration of PCR fragments using a Nanodrop (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: PCR products of candidate genes were purified using the Zymoclean Gel DNA Recovery kit (Zymo Research) and then cloned into pENTR/D/SD entry vector using the In-Fusion cloning kit (Takara) ...
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bioRxiv - Genetics 2023Quote: ... leftover PCR product was purified using the DNA Clean & Concentrator™-5 kit (Zymo Research #D4067). The attB PCR product was cloned into an empty pDONR™221 vector ...
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bioRxiv - Cell Biology 2023Quote: ... and the PCR amplicon was purified using the DNA Clean & Concentrator-5 (Zymo Research, Irving, CA). The amplicon was incubated in 10X T4 ligase buffer (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... and further cleaned up by a OneStep PCR Inhibitor Removal Kit (D6030; Zymo Research, Irvine, CA) before PCR amplification ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative RT-PCR: RNA extraction was carried out with the ZR RNA MiniPrep kit (ZYMO RESEARCH) and cDNA was synthesized from 10 ng/µl RNA using the High Capacity cDNA Reverse Transcription Kits (Life Tech) ...
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bioRxiv - Microbiology 2024Quote: ... PCR fragments and the fused construct were column purified (Zymo DNA Clean-Up and Concentration kit). The purified construct was phosphorylated with T4 polynucleotide kinase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were cleaned and concentrated using a DNA Clean and Concentrator-25 kit (Zymo Research). DNA concentrations were determined using a NanoDrop spectrophotometer (ThermoFisher ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA fragment was generated by PCR amplification and purified by DNA Clean & Concentrator Kit (Zymo Research). gRNA plasmids were constructed according to the ‘gRNA-tRNA Array Assembly’ methods described in the Multiplex MoClo Toolkit11.
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bioRxiv - Systems Biology 2024Quote: ... PCR samples were cleaned up using the Zymo DNA Clean and Concentrator kit (Zymo Research, USA) and a second PCR was performed to add a second index and flow cell attachment sequence ...
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bioRxiv - Biochemistry 2024Quote: ... PCR product was then purified using a Zymo Genomic DNA Clean & Concentrator kit (Zymo Research D4011) and the DNA concentration was determined using a Nanodrop One (Thermo Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... The resulting PCR products were purified using the DNA Clean & Concentrator Kit (Zymo Research, Irvine, USA) and cloned via Gibson assembly into pPREx2 plasmid digested with NdeI and NheI 96 ...
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bioRxiv - Genetics 2021Quote: ... 100µl of the gDNA solution was purified using the Zymo Genomic DNA Clean and Concentrator –10 kit (Zymo Research #ZD4010) following the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: Two hundred microliters of lysozyme solution (50 U/μL Ready-lyse lysozyme [Lucigen, WI, USA], 2 U/mL zymolyase [Zymo Research ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were scraped into solution and total RNA was purified using Zymo Quick-RNA Miniprep kit (Zymo Research, R1054). Of the isolated total RNA ...
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bioRxiv - Neuroscience 2023Quote: ... and RNA was isolated by miRNeasy Mini Kit solutions (Qiagen217004) and Zymo-Spin IIICG Columns (Zymo Research C1006-50-G) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... The solution was diluted to 10% RNALater by the addition of 270 µL RNase-free water (Zymo Research, Irvine, CA), vortexed gently ...
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bioRxiv - Biochemistry 2022Quote: ... the protein solutions were applied to a spin column containing Strep-Tactin XT Superflow suspension (Zymo Research, Irvine, CA, USA). After washing the column in a buffer containing 50 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Genetics 2023Quote: ... 100μl of the gDNA solution was purified using the Zymo Genomic DNA Clean and Concentrator –10 kit (Zymo Research #ZD4010) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... High melanin content was removed with OneStep™ PCR Inhibitor Removal Kit (Zymo Research Corporation, Irvine, CA). Direct-zol™ RNA MiniPrep w/ Zymo-Spin™ IIC Columns (Zymo cat # D4019 ...
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bioRxiv - Genomics 2020Quote: ... and gel-purified the PCR products (i.e. inserts) using the Zymoclean Gel DNA Recovery Kit (Zymo Research). We used the NEBuilder HiFi DNA Assembly Kit (NEB ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products and digested plasmid were cleaned using the Zymo DNA Clean & Concentrator kit (Zymo Research) and assembled using the S ...
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bioRxiv - Genomics 2020Quote: ... The remaining PCR product was processed using a ZR-96 DNA Clean & Concentrator-5 kit (Zymo Research) and submitted to Cornell Core Biotechnology Resource Center Genomics Facility for DNA sequencing ...
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bioRxiv - Microbiology 2020Quote: ... All genomic DNA used for screening PCR was isolated using the Quick-DNA miniprep kit (Zymo Research). The resulting knockout strain was designated Variovorax CL14ΔHS33.
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bioRxiv - Biochemistry 2021Quote: 96-well PCR plates with 50 µL of DH10B ‘Mix & Go Competent Cells’ (prepared using Zymo T3001) in each well were prepared beforehand so that high-throughput transfers could be done using multichannel pipettes or the Tecan Fluent ...
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bioRxiv - Systems Biology 2021Quote: ... 2μL of PCR products from each well were pooled and purified using the DNA Clean & Concentrator (Zymo) and eluted in water ...
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bioRxiv - Microbiology 2022Quote: ... These crude extracts were subsequently purified by passage through a OneStep PCR Inhibitor Removal column (Zymo, D6030) and purity was assessed by UV-spectra ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR products were cleaned up and concentrated using a DNA Clean-up and Concentration Kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... PCR products for transformation were purified and concentrated with the Zymo DNA Clean & Concentrator kit (Zymo Research) with a final elution in molecular biology grade water (Corning).
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bioRxiv - Synthetic Biology 2020Quote: ... DNA purification/concentration following PCR and restriction digests were performed with DNA Clean & Concentrator TM-5 (Zymo). Nucleic acid concentrations were measured with a NanoDrop 2000c spectrophotometer ...
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bioRxiv - Immunology 2021Quote: ... Amplicons were purified directly from PCR reaction mix by using DNA Clean & Concentrator Kits (Zymo Research, #D4013) or recovered from agarose gel by using Zymoclean Gel DNA Recovery Kit (Zymo Research ...
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bioRxiv - Genomics 2021Quote: ... PCR products were purified with the Zymo DNA Clean & Concentrator Kit™ (Zymo Research, Irvine, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... Extracted DNA was purified using a OneStep™ PCR Inhibitor Removal kit (Zymo Research, Irvine, CA, USA) and subsequently quantified using an Qubit dsDNA HS assay (Thermo-Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... PCR products were purified using a Genomic DNA Clean and Concentrate Kit (Zymo Research, Irvine, CA, USA), then cloned using the pGEM T-Easy kit and manufacturer’s protocol (Promega ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR amplified linear template DNA sequences were purified using DNA Clean and Concentrator-25 kit (Zymo Research). The resulting purified DNA was eluted in nuclease-free ultrapure water ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were purified using a ZR-96 DNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA), and replicate products were then pooled ...
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bioRxiv - Immunology 2024Quote: ... Bisulfite conversion was confirmed by PCR using Universal Methylated Human DNA Standard with hMLH1 Primers (Zymo Research). Converted DNA was then applied to an Illumina Infinium MethylationEPIC850 BeadChip (865918 probes ...
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bioRxiv - Synthetic Biology 2023Quote: ... All PCR products were purified via gel extraction using the Zymoclean Gel DNA Recovery kit (Zymo Research). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were purified by means of the DNA Clean and Concentrator-25 spin column kit (Zymo Research Corp. ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 20 μL PCR products were then purified with DNA Clean & Concentrator-5 (Zymo Research, Irvine, CA, USA). Amplicons were eluted with 25 μL of nuclease-free water (Thermo Scientific) ...
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bioRxiv - Genomics 2023Quote: The PCR products were pooled per growth media condition and purified with DNA Clean & Concentrator (Zymo Research). The second PCR was performed using PrimeSTAR GXL polymerase (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... Emulsion PCR products were purified using a scaled-up protocol of OligoClean and Concentrator Kit (Zymo Research). The resulting PCR products were analysed by 10% polyacrylamide gel electrophoresis after staining with GelGreen® dye (Biotium ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were agarose-gel–purified and further concentrated by a DNA clean & concentrator kit (Zymo Research). DNA libraries prepared from the HindIII-treated and untreated samples of the same donor were mixed in a 3:2 ratio ...
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bioRxiv - Genetics 2024Quote: ... The PCR amplified DNA was extracted from the agarose gel using Zymoclean gel recovery kit (Zymo Research) and cloned into the TIGRE targeting plasmid using Gibson assembly to create a ‘donor homology construct’ for generation of stable cell lines ...