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Citations for Zymo Research :
1 - 50 of 437 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... followed by neutralization with HCl (5M) and cDNA purification using the Zymo Oligo Clean & Concentrator kit (Zymo Research). The sample was eluted with water and vacuum concentrated to 5 ul ...
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bioRxiv - Microbiology 2024Quote: ... 750 µl of lysis solution (ZymoBiomics Lysis Solution) and 19 µl 20 mg/ml proteinase K solution in storage buffer (Zymo Research, Irvine, CA, USA) were added into each tube and mixed ...
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bioRxiv - Cell Biology 2021Quote: ... Transfections were performed with PEI and 2-5 µg of transfection grade DNA (ZymoPure Plasmid MaxiPrep Kit, Zymo) per dish as described in above ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR product was purified by PCR purification kit (Zymo Research, D4013) and sent to Azenta Life Sciences for Sanger sequencing ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified using Zymo PCR purification kit (Zymo Research) and directly transformed into Stellar™ chemical competent cells (Takara Bio ...
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bioRxiv - Microbiology 2020Quote: ... a nucleic acid stabilizing solution from Zymo Research (R1100) ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were PCR purified using the DNA Clean and Concentrator kit (Zymo Research), then transformed into One Shot TOP10 competent cells (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2020Quote: ... PCR products were pooled and cleaned up with the Zymo PCR Cleanup Kit (Zymo) before performing 2% agarose gel separation and extraction with the Zymo Gel Extraction Kit following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR amplicons were purified using the Zymo PCR purification kit® (Zymo Research©). Plasmid constructions were performed using In-fusion® HD-cloning kit (Takara© ...
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bioRxiv - Microbiology 2024Quote: ... Correct amplicons were isolated by Zymo PCR purification kit (Zymo Research). The PCR products were cloned into a PCR linearized pEcgRNA-guide plasmid to make the pEcgRNA-guide-RT plasmid using In-fusion® HD-cloning kit (Takara Bio ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection-grade plasmid DNA was isolated from each flask using the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The second endpoint PCR amplification was subjected to a PCR DNA purification kit (Zymo Research) and the DNA was submitted for sequencing as described by Christensen and colleagues ...
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bioRxiv - Developmental Biology 2022Quote: ... GATA-3 and RORC were generated by PCR with ZymoTaq PCR Master Mix (Zymo Research) using the primers listed in Table 1 ...
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bioRxiv - Genomics 2021Quote: ... gDNA was purified prior to PCR using the Zymo OneStep PCR Inhibitor Removal Kit (Zymo), per the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... and the resulting PCR products were purified via a PCR Clean and Concentrator kit (Zymo). Cloning of TF genes into the pJET ...
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bioRxiv - Genetics 2024Quote: ... gDNA was purified prior to PCR using the Zymo OneStep PCR Inhibitor Removal Kit (Zymo), per the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2021Quote: ... and potential PCR inhibitors were removed with the OneStep-96TM PCR Inhibitor Removal Kit (Zymo Research). RNA concentrations were quantified with the Qubit RNA Assay kit (Life Technologies ...
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bioRxiv - Genetics 2020Quote: ... gDNA was purified prior to PCR using the Zymo OneStep PCR Inhibitor Removal Kit (Zymo, D6030), per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... PCR product was purified using a Zymo PCR DNA Clean and Concentrator Kit (Zymo cat# D4030), and 200 ng of annealed PCR product was subject to T7 Endonuclease I digestion for 15 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... After PCR product purification (Zymo Research), the fragments of interest were inserted into linearized ZP446 using Gibson Assembly ...
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bioRxiv - Microbiology 2023Quote: ... pellet resuspended with 2 ml Host Depletion Solution (Zymo) and transferred without the beads to two 1.5 ml tubes per sample ...
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bioRxiv - Bioengineering 2024Quote: ... After adding 700 μL of wash solution (Zymo Research), the column was centrifuged again at 16,000 ×g for 30 s to remove the solution ...
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bioRxiv - Microbiology 2021Quote: ... PCR inhibitors were then removed from the extracted DNA using the OneStep PCR inhibitor removal kit (Zymo) following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: ... 15 μl of PCR products were pooled and purified using PCR Purification Kit (D4013, Zymo Research Co.). Depending on the amplicon ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the Zymogen Clean and Concentrator PCR-cleanup kit (Zymo Research, Irvine, CA), and the recovered DNA fragments were ligated together to form closed circular DNA using T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Developmental Biology 2024Quote: Promoter fragments from the GATA2 gene were generated by PCR with ZymoTaq PCR Master Mix (Zymo Research) using the primers listed in Table 2 ...
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bioRxiv - Bioengineering 2024Quote: ... the solution was transferred to RNA extraction columns (Zymo Research), and the solvents were removed by centrifugation at 16,000 ×g for 30 s at room temperature ...
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bioRxiv - Bioengineering 2021Quote: Colony PCR was performed on bacterial cultures and the PCR product purified using the DNA Clean and Concentrator kit (Zymo). Purified PCR products were sequenced at Genewiz (USA).
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bioRxiv - Molecular Biology 2023Quote: ... PCR product sizes were verified on a 1% agarose gel prior to PCR clean up (DNA Clean & Concentrator-5, Zymo). A DNA fragment for transcription of lpp (lpp sequence preceded by aforementioned T7 transcription-enabling sequence ...
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bioRxiv - Molecular Biology 2023Quote: The PCR products containing the aforementioned various RNA constructs were cleaned using a PCR DNA clean up kit (Zymo Research), and used in Gibson assembly reactions (5x Gibson buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR amplicons (280 bp) were purified using the Clean and Concentrator PCR purification kit (Zymo Research, Irvine, CA, USA) and their quality verified on a 1.5% w/v agarose gel ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions yielding single amplicons as judged by agarose gel electrophoresis were purified using a PCR clean up kit (Zymo Research) and sent for DNA sequencing ...
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bioRxiv - Bioengineering 2022Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned 5MCS3 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the DNA template via in vitro transcription (IVT ...
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bioRxiv - Synthetic Biology 2023Quote: ... The DpnI-treated inverse PCR vector and PCR product inserts were column purified using a DNA Clean and Concentrate Kit (Zymo Research). Inserts were added with 2-fold molar excess to 75 ng of the vector ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA template for modRNA synthesis was PCR amplified from the successfully cloned modRNAc1 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research). ModRNA was synthesized from the PCR DNA template via in vitro transcription (IVT ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR were performed and PCR amplicons were used to conduct gel running and purified by DNA Clean & Concentrator kit (Zymo Research, # D4031). A-tailing were then performed on the purified PCR products following the NEB protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... we used limited-cycle PCR to amplify the oligopools (Twist Biosciences) and cleaned up the PCR product using DNA oligo purification kits (Zymo Research, D4003). Then ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were purified (Zymo DNA Clean & Concentrator kit) and then combined ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were column purified (Zymo DNA clean & concentrate) and digested with EcoRI and BamHI alongside digestion of the base plasmid ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were column purified (Zymo DNA clean & concentrate). Successful Gibson Assembly reactions occurred using a 7:1 molar ratio of insert:backbone.
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were column-purified (Zymo Research, Irvine, CA) and eluted in 30 μL ...
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bioRxiv - Genetics 2021Quote: ... The PCR was performed with ZymoTaq PreMix (ZYMO RESEARCH) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The PCR was performed with ZymoTaq PreMix (ZYMO RESEARCH) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The PCR was performed with ZymoTaq PreMix (ZYMO RESEARCH) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... PCR products were column cleaned (Gel cleanup kit, Zymo), and pooled based on relative concentration ...
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bioRxiv - Cell Biology 2021Quote: ... and purified with a PCR purification kit (Zymo Research). TRIzol™ (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... and purified using a PCR purification kit (Zymo Research), and then used for in vitro transcription with the MEGAshortscript T7 kit (Ambion) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 µl of each module (PCR product + Zymo Shield) (6 in total ...
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bioRxiv - Microbiology 2020Quote: ... Amplified insertions were PCR purified (DNA purification kit, Zymo) and subjected to Sanger sequencing by MCLAB ...
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bioRxiv - Microbiology 2022Quote: ... PCRs were purified (Zymo Research Clean and Concentrator kit) and pooled for sequencing on an Illumina MiSeq with 150 bp single end reads ...