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Citations for Zymo Research :
251 - 293 of 293 citations for 6 N CARBETHOXY 3 CHLORO 7 8 DIHYDRO 5H PYRIDO 4 3 C PYRIDAZINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at 37°C for 1 hour and then purified with Zymo DNA Clean & Concentrator-5 (Zymo Research) following the PCR product protocol and eluting DNA into 10.5 μL of water ...
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bioRxiv - Molecular Biology 2023Quote: ... for 30 min at 37°C and further purified by using a Zymo DNA Clean and Concentrator column (Zymo Research, D4033).
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bioRxiv - Microbiology 2023Quote: ... Prototrophic colonies were grown in uracil dropout medium (MilliporeSigma) at 30 °C and 250 rpm and the plasmid was purified using the Yeast Plasmid MiniPrep II Kit (Zymo Research). The plasmid was transformed into TransforMax EPI300TM E ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× RNase H buffer overnight at 37 °C before immunoprecipitation and recovered using the Oligo Clean & Concentrator kit (Zymo Research). For m1A-DIP ...
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bioRxiv - Molecular Biology 2024Quote: ... treated with 1.25 units of RQ1 DNase for 30 minutes at 37°C and RNA recovered using an RNA Clean & Concentrator-25 spin column kit (Zymo Research), following manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... at 37°C for 1 hr and purified again by illustra microspin G-50 column or Direct-zol RNA Miniprep kits (Zymo Research) before transfection (Supplemental Fig ...
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bioRxiv - Genetics 2023Quote: ... Transposition reactions were incubated for 30 min at 37°C while shaking at 1,000 rpm and cleaned using DNA Clean & Concentrator-5 (Zymo Research, D4003). Transposed libraries were amplified in 50 μl PCRs (0.5 μl Q5 DNA polymerase ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatant was reverse-crosslinked overnight (12-16 hours) at 65°C prior to ChIP DNA isolation using Zymogen ChIP DNA Clean and Concentrator Kit (Zymo Research, D5205). For ChIP-seq ...
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bioRxiv - Genomics 2022Quote: ... The corresponding amount of polyA RNA were incubated with 1X fragmentation buffer at 95 °C for 1 minute to generate ~300 nt of RNA fragment followed by column purification (Zymo Research, R1016) as described before [23] ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 30 min and concentrated to 8μL using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA). Full protocol for nucleic acid extraction and subsequent cDNA generation is available at https://www.protocols.io/view/sequence-independent-single-primer-amplification-o-bckxiuxn.
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then pelleted and stored at −20°C for at least six hours prior to extraction using Zymo Yeast Plasmid Miniprep II (Zymo Research # D2004), following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: ... for 1 h at 37°C to remove free oligonucleotide and purified by ethanol precipitation/ or the DNA Clean and Concentrator 5 kit (Zymo Research, D4003T). Libraries were resolved on a 2% MetaPhor agarose (Lonza ...
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bioRxiv - Genetics 2022Quote: ... 30 ng of purified dsDNA was tagmented at 55 °C for 15 min and purified using DNA Clean & Concentrator-5 kit (Zymo Research, D4004) The tagmented DNA was PCR amplified using Nextera primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were incubated for 10 min at 37°C before quenching using a Zymo Genomic DNA Clean & Concentrator 10 (Zymo Research D4011) kit according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The transcribed product was treated with DNase I for 30 min at 37°C and then purified using RNA Clean and Concentrator Kit (Zymo Research #R1016).
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bioRxiv - Cell Biology 2023Quote: ... The crosslinking was reversed at 65 °C and the samples were purified using the ChIP DNA Clean and Concentrator kit (Zymo Research, D5205). The DynaMagTM-2 Magnet (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were fragmented to around 300nt length at 95°C for 1min and purified using RNA clean and concentrator (Zymo research, R1016). The fragmented RNA was then subjected to a 5’ dephosphorylation reaction at 37°C for 30min by adding rSAP (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 1 h and further cleaned up by using a Zymo RNA Clean and Concentrator-5 purification kit (Zymo Research, R1016). All the oligos used in the library preparation are noted in Supplementary Table 6a.
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bioRxiv - Genomics 2023Quote: ... Single colonies were used to inoculate ampicillin liquid cultures grown overnight with shaking at 37°C and plasmids were subsequently purified with the ZR Plasmid Miniprep kit (Zymo Research #D4016). The purified plasmids were screened by Sanger sequencing (GeneWiz ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 mL of an overnight culture of EC1000 grown in LB media at 37°C was transferred to 50 mL of ZymoBroth (Zymo Research #M3015) and cultured for 15–16 hours at 22°C with shaking to obtain a culture of OD600=0.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... Following phenol/chloroform and EtOH precipitation the pellets were dissolved in 300ul 10mM Tris pH7.5 and DNA was further purified using 4 Zymo columns per sample (Zymo, D4014). Each sample was eluted in 200ul total of 10mM Tris pH7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Samples were then transferred in 4 ml tubes and processed for total RNA extraction with RNA micro kit (ZYMO REAGENT) according to company protocol ...
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bioRxiv - Microbiology 2023Quote: ... 4 ml of the cultures were used for total RNA extraction using the Direct-zol RNA extraction kit (Zymo Research) and TRI Reagent (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: Tumors were then harvested 4 weeks post-injection and processed using Quick-DNA midiprep plus kit (Zymo Research Cat. #D4075). For each processed tumor ...
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bioRxiv - Immunology 2021Quote: MSP1-specific B cells that successfully expanded in culture were collected by centrifugation (5 min. at 250 × g and RT) and stored at -70°C in 50 µl Tri-Reagent (Zymo #R2050-1-200). Heavy and light chain variable regions were amplified from MSP1-specific B cells by cDNA synthesis and a series of PCR reactions ...
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bioRxiv - Microbiology 2020Quote: The respiratory samples were prospectively stored at −80°C after stabilization by mixing an equal volume of DNA/RNA Shield (2X concentrate; Zymo Research, Irvine, CA). The thawed samples were centrifuged at 20,000×g for 2 min ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Total RNA from archived (stored at -80°C) American alligator whole blood was isolated using the Quick-RNA Whole Blood Kit (Zymo Research, Irvine, CA). Samples from both LW and CFR were selected randomly based only on the availability of samples ...
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bioRxiv - Microbiology 2021Quote: ... Each strain was grown in 1 ml of LB+CA100 at 30°C for 48 hours and genomic DNA was extracted using a Quick-DNA High Molecular Weight kit (Zymo Research, cat#D6060) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... chromatin was de-crosslinked for 15 minutes at 65°C and purified using the ChIP DNA Clean & Concentrator kit (Zymo Research, Freiburg, Germany). Binding of Msh2 ...
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bioRxiv - Microbiology 2021Quote: ... The pellet was frozen at -20°C until DNA extraction using a Zymo fecal/soil DNA extraction kit according to enclosed directions (Zymo Research, Irvine, CA). After sonication ...
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bioRxiv - Microbiology 2020Quote: ... isolates were grown shaking in SG broth at 37°C and genomic DNA isolated using the Quick-DNA Fungal/Bacterial miniprep kit (Zymo Research, Irvine, CA) according to the manufacturer's suggested protocol.
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bioRxiv - Genomics 2020Quote: ... DNA in the initial 20-μL nucleic acids solution was digested by adding 16-μL DNase I (1 U/μL) plus 4-μL 10X DNase I buffer (DNase I set; Zymo Research), incubating for 15 min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... 4-S-UTP containing RNA was eluted in 100mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research) with in-column DNAse reaction to eliminate traces of genomic DNA ...
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bioRxiv - Microbiology 2019Quote: ... Approximately 1 ml of sediment slurry was serially diluted and 100 μL was plated in 10−4 dilution in triplicate on MB2216 agar plates using rattler beads (Zymo #S1001); colony forming units (CFU ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from Vero cells after 4 days post infection using the Direct-zol RNA isolation kit (Zymo Research). 1 μg of RNA isolate was subjected to rRNA removal with the NEBNext rRNA Depletion Kit (NEB ...
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bioRxiv - Bioengineering 2022Quote: The isolated yeast populations obtained after round 4 were subjected to colony screen and plasmid recovery by using Zymoprep kit (Zymo Research). The plasmids were transformed into E ...
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bioRxiv - Genomics 2020Quote: ... 4-SUTP containing RNA was eluted in 100 mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research) with in-column DNAseq reaction to eliminate traces of genomic DNA ...
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bioRxiv - Microbiology 2020Quote: ... Samples were further incubated 10 min at 75° C and quickly placed on ice before being cleaned and re-eluted using the RNA Clean & Concentrator kit (Zymo Research, Irvine, CA, USA) and stored at −80° C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Grow white colonies overnight in 5 ml LB+Carb with shaking at 225 rpm at 37°C i) Extract plasmid DNA using the Zippy Plasmid Miniprep Kit (Zymo Research Cat. # 11-308) following manufacturer protocol with elution 75 µl nuclease-free water ...
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bioRxiv - Genomics 2020Quote: DNA was extracted using 4-10 unstained slides of FFPE tissue using the Pinpoint Slide DNA Isolation system (Zymo Research, Orange, CA). In brief ...
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bioRxiv - Plant Biology 2019Quote: Total RNA from 4 weeks old Arabidopsis thaliana WT Col-0 and mta mutant plants was isolated using Direct-zol™ RNA kit (Zymo Research). RNA was quantified by Qubit RNA Assay Kit (Life Technologies ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were then harvested by centrifugation at 3500g for 4 min and prepared for transformation using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA). For plasmid transformations ...
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bioRxiv - Cancer Biology 2023Quote: DNA from 32 skull base chordomas and 4 NP samples were bisulfite converted with EZ-96 DNA Methylation kit (Zymo Research, Orange, CA, USA) and used for genome-wide DNA methylation profiling with Methylation EPIC (Illumina ...