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Citations for Zymo Research :
51 - 100 of 293 citations for 6 N CARBETHOXY 3 CHLORO 7 8 DIHYDRO 5H PYRIDO 4 3 C PYRIDAZINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl RNA from total or immunopurified ribosomes were 3’ dephosphorylated and 5’ phosphorylated as described above 30 and was selectively recovered with RNA Clean and Concentrator-5 (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... the total RNA was extracted from active and tun samples (3 replicates) using Direct-Zol RNA MicroPrep Kits (Zymo Research), and the sequencing library was constructed using SMARTer v4 Ultra Low Input RNA Kit for Sequencing (Clonetech ...
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bioRxiv - Developmental Biology 2019Quote: ... The Pgap2 exon 3 variant was Sanger sequenced using the Zymo DNA clean & Concentrator kit (Zymo Research Corporation, Irvine, CA).
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bioRxiv - Genetics 2022Quote: Yeast colonies containing bait and prey constructs that grew on Trp−/Leu−/His− + 25 mM 3-Amino-1,2,4-triazole (3AT) selection plates were extracted using the Zymoprep Yeast Plasmid Miniprep II (Zymo Research, D2004) followed by Sanger sequencing for gene identification ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: DNA libraries for Nanopore sequencing were prepared by extracting plasmid DNA from ~1 × 109 cells (input library, 30 ml culture) or ~3 × 107 cells (output populations, 1 ml culture) using Zymoprep Yeast Plasmid Miniprep (Zymo). One freeze-thaw cycle (at −80°C ...
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bioRxiv - Genomics 2023Quote: ... 3 colonies were randomly selected and further expanded for plasmid DNA purification (Zyppy™ Plasmid Purification Kit; Zymo research #D4019). Extracted DNA was then sequenced to verify the integrity of the cloned fragments.
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bioRxiv - Plant Biology 2023Quote: RNA was extracted from 3 biological replicates of 100mg of frozen tissue of each accession using the Quick-DNA/RNA Miniprep kit (Zymo) following manufacturer’s instructions with the following modifications ...
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bioRxiv - Microbiology 2020Quote: ... and mRFP1 was PCR amplified using primers listed in Table 3 and purified using DNA Clean and Concentrator-5 kit (Zymo Research). pUC19 was digested with PstI and pBAD18 was digested with EcoRI and HindIII ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
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bioRxiv - Genetics 2019Quote: 25-30 eyes were dissected from both WT and pitx2ΔCE5-7 eye zebrafish embryos at 3 dpf in Ringer buffer and genomic DNA was purified using Quick-DNA microprep Plus kit (ZYMO Research). Eyes were resuspended in Solid Tissue buffer containing Proteinase K and incubated at 55°C for 6 hours ...
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bioRxiv - Microbiology 2019Quote: ... at 48 hpe cells were washed 3 times with PBS to eliminate extracellular virus and total RNA was extracted using Direct-zol RNA MiniPrep Plus (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Individual 50 μL reactions were run on a 3% agarose gel and libraries were purified using the Zymoclean Gel DNA Recovery kit (D4007, Zymo Research). Sequencing was performed using standard Illumina instructions ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 10 cm plates of SKMEL5 cells with 3 biological replicates per condition (DMSO, 100 nM binimetinib, 1 μM binimetinib) using Direct-zol RNA miniprep kit (Zymo Research), as previously described (24) ...
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bioRxiv - Genomics 2021Quote: ... The nascent RNA was purified by adding 3 volumes of TRIzol and processed with the Direct-zol RNA Miniprep kit (Zymo Research) with addition of 1/100 volume of 100 mM DTT to each supplied wash buffer.
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bioRxiv - Microbiology 2021Quote: ... and genomic DNA was extracted from 3 mL of overnight (TSB) broth culture using the ZR Duet DNA/RNA MiniPrep Plus kit (Zymo Research) following manufacturer’s protocol for the “suspended cells” option ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
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bioRxiv - Microbiology 2022Quote: ... The high molecular weight bands were excised with a razor and dissolved in 3 volumes of Zymo Agarose Dissolving Buffer (Zymo D4001) at 50°C ...
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bioRxiv - Neuroscience 2022Quote: ... The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080; Zymo Research). The kit columns were substituted with Zymo-Spin IC Columns (C1004-50 ...
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bioRxiv - Neuroscience 2022Quote: ... the resulting solution was incubated with 3 volumes of TRI-reagent at room temperature for 2 hours (R2050-1-50; Zymo Research). The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080 ...
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bioRxiv - Cancer Biology 2023Quote: ... change of reverse transcription primers for compatibility with standard Illumina sequencing primers 3) cDNA clean-up prior to library amplification performed with the DNA Clean & Concentrator-5 kit (Zymo, #D4013) 4 ...
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bioRxiv - Immunology 2021Quote: ... Samples were stored in RNAlater (Invtrogen) for 24 hours at 4°C and then homogenized in TRI Reagent (Zymo Research) with a beadbeater ...
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bioRxiv - Plant Biology 2020Quote: Genomic DNA was extracted from aerial parts of 3-week-old seedlings using Quick-DNA Plant/Seed Miniprep Kit (Zymo research, D6020) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... to block the 3’-ends from ligation and were then size-selected to 200 ≥ nt (DNA Clean & Concentrator™-5, Zymo Research). 5’ adaptors were attached using T4 RNA ligase (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: A PCR of all constructs using forward primer pTD2F (5’-TTGGCACCAAAATCAACGGG–3’) and reverse primer SP6 was carried out with SuperFi DNA Polymerase (ThermoFischer Scientific, USA) and agarose gel purified (Zymo Research, USA). These primers amplify the gene insert ...
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bioRxiv - Genetics 2023Quote: ... Cells were cultured for 3-5 days post-transfection after which genomic DNA was extracted using the Quick-DNA Microprep Kit (Zymo Research Corporation).
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bioRxiv - Genetics 2023Quote: ... 30 samples were collected (5 timepoints x 2 genotypes x 3 replicates) and RNA was extracted using a Direct-zol RNA Miniprep (Zymo Research R2061) using TRIzol Reagent (ThermoFisher Cat # 15596026 ...
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bioRxiv - Zoology 2024Quote: ... Clean-up used a combination of two kits: the beads from the Select-a-size Magbead kit (Zymo Cat# D4084-3-10) and the buffers from Genomic DNA Clean & Concentrator -25 (Zymo Cat# D4064) ...
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bioRxiv - Physiology 2024Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick-RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
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bioRxiv - Microbiology 2023Quote: ... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...
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bioRxiv - Physiology 2023Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick- RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
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bioRxiv - Microbiology 2020Quote: ... Samples were centrifuged 1 min at 12000 g at 4°C and RNAs were extracted from supernatants with Direct-zol RNA Mini Prep kit (Zymo Research), following manufacturer’s protocol and including the optional DNAse treatment step ...
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bioRxiv - Bioengineering 2023Quote: ... The lysates were centrifuged at 5000 x g for 10 min at 4 °C and supernatant added to gDNA extraction columns (Zymo Research). The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research ...
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bioRxiv - Molecular Biology 2022Quote: ... the aqueous phase was diluted back in TRizol reagent (1:3) to proceed with RNA purification by zymodirect RNA kit (Zymo research, cat. R2051). The quality of RNA samples was assessed using Agilent 2200 TapeStation (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... one set of replicates (total of 3 replicates per treatment) was harvested for DNA and RNA using ZR-Duet DNA/RNA MiniPrep (Plus) kit (Zymo Research Cat # D7003) following manufacturer’s protocol ...
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Phage-centric ecological interactions in aquatic ecosystems revealed through ultra-deep metagenomicsbioRxiv - Microbiology 2019Quote: ... were cut in small pieces (≅ 3–5mm) using sterile scissors and processed for DNA extraction using the ZR Soil Microbe DNA MiniPrep kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) and 3 swabs (2 for metagenomic DNA isolation, 1 for storage) moistened with DNA/RNA shield (Zymo Research, Cat. No. ZYR.R1100-250). Swabbing was performed for 2 min in each site and stored in respective storage liquids (i.e ...
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bioRxiv - Plant Biology 2022Quote: ... Then, samples were centrifuged (14000 g, 3 min) and the supernatants were transferred to silica columns to bind DNA (Zymo Research, Irvin, California, USA). After 1 min of incubation ...
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bioRxiv - Microbiology 2019Quote: ... total DNA or total RNA was extracted from 1−3 × 106 cells using the Quick-DNA or Quick-RNA Miniprep Kits (Zymo Research, Irvine, CA USA) respectively ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mL of suspended biomass was collected in triplicate from the mixed tank and PBR effluent and stored in 5 mL polypropylene transport tubes (FisherbrandTM, USA) prefilled with 3 mL DNA/RNA ShieldTM (Zymo Research corp., CA, USA). The samples were briefly vortexed and stored at -20 °C until they were shipped to University at Buffalo (UB ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then treated with Turbo DNAse 2 to 3 times and then purified with the RNA Clean and Concentrator-5 Kit (Zymo Research Cat#: 11-325) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and 7) ZymoBIOMICS™ DNA Microprep kit (Zymo Research, California, USA) with modifications ...
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bioRxiv - Systems Biology 2022Quote: ... the second sample was stored at RT for 3 – 5 days and the third sample was collected in a Zymo research tube (Zymo Research Inc., Irvine, CA, USA) containing 9ml DNA/RNA shield and stored at RT for 3 – 5 days before storage at −80 °C ...
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bioRxiv - Microbiology 2020Quote: DNA from the meconium samples (N = 25) and corresponding negative controls (N = 11) were extracted using ZymoBIOMICS DNA Miniprep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The Methylated-DNA IP Kit from Zymo (Cat. N° D5101) was applied according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... cells collected by centrifugation (6000 xg, 10 min, 4°C) and DNA extracted using the ZR Fungal/Bacterial DNA Kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... with Zymo-Spin IC Columns (Zymo Research, Cat. N: C1004-250).
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bioRxiv - Genomics 2020Quote: ... sheared and biotinylated fully methylated pUC19 (Zymo Research, Cat. N: D5017) and unmethylated lambda DNA (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... 8 were performed with the YeaStar RNA Kit (Zymo Research) following the instructions of the given protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 0.2μL of 25mg/mL RNAse A (Zymo research #E1008-8) was used for each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... or DNA/RNA Shield (Zymo Research; Cat: R1100-8-S) and stored at −80°C until further use.