Labshake search
Citations for Zymo Research :
201 - 250 of 293 citations for 6 N CARBETHOXY 3 CHLORO 7 8 DIHYDRO 5H PYRIDO 4 3 C PYRIDAZINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... C shaker for 12h-16h prior to harvesting for plasmid purification using ZymoPURE II Plasmid Midiprep kit (Zymo D4200). Based on the colony forming units from the small volumes in the bacterial plates ...
-
bioRxiv - Molecular Biology 2024Quote: Liver samples were collected at euthanasia and stored at -80°C in 1X DNA/RNA Shield™ from Zymo Research ...
-
bioRxiv - Genetics 2023Quote: ... individual array bearing worms were isolated and lysed in 4µl of EB (Zymo, Cat. No.: D3004-4-16) buffer with 1mg/ml proteinase K (NEB) ...
-
bioRxiv - Genetics 2023Quote: ... we extracted DNA from 2–4 dpf whole larvae using the Quick-DNA Microprep Kit (D3021, Zymo Research). We then performed a PCR for GFP as well as a ‘junction PCR’ to amplify a region including both mitfa and GFP to ensure integration at the intended locus.
-
bioRxiv - Microbiology 2024Quote: ... hydrophila MAH-4 was pelleted and genomic DNA obtained using Zymo DNA Miniprep (Zymo Research, Irvine, CA, USA). Long reads and short paired end reads (2 x 151 bps ...
-
bioRxiv - Developmental Biology 2020Quote: ... and stored in −80°C until extraction of total RNA extraction using the Direct-zol™ RNA kit (Zymo Research). The optical density value of total RNA was measured using the NanoDrop spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2019Quote: ... The reaction was incubated 10 minutes at 55°C followed by purification with DNA Clean & Concentrator-5 kit (Zymo Research) and elution in 21 µL of water ...
-
bioRxiv - Bioengineering 2020Quote: ... at 37°C for 1 hour to remove the gblock template then purified using a RNA spin column (Zymo Research). Purified RNA was then treated with 200 units of calf intestinal phosphatase (New England Biolabs ...
-
bioRxiv - Genomics 2022Quote: ... The DNA was digested with 0.5 mg/ml Proteinase K for 1 hour at 65°C and then purified using the ChIP DNA Clean & Concentrator kit (Zymo) and quantified with QUBIT ...
-
bioRxiv - Immunology 2021Quote: ... Samples were incubated at 37°C for 60 minutes and purified using the Zymo DNA Clean and Concentrator-5 Kit (Zymo). Fragments were amplified according to the previously published protocol ...
-
bioRxiv - Evolutionary Biology 2019Quote: 208 female beetles (Table 1) p stored at −80°C were homogenized in Tri-Reagent® (Zymo Research, California, USA) using an electric bead mill ...
-
bioRxiv - Genomics 2020Quote: ... was added and incubated for 60 min at 50 °C and the DNA was purified using the Oligo Clean & Concentrator kit (Zymo). DNA was analyzed for oxidized 5mC modifications using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS ...
-
bioRxiv - Molecular Biology 2020Quote: ... Reactions were incubated for 16 hours at 16 °C and were terminated by cleaning with RNA Clean & Concentrator (Zymo Research). Crosslink reversal was done by irradiating the RNA on ice with 2.5 KJ/m2 254 nm UVC using a CL-1000 crosslinker (UVP).
-
TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... Crosslinks were reversed at 65°C overnight and DNA was purified with ChIP DNA Clean & Concentrator kit (Zymo Research, D5205). Sequencing libraries were prepared using the KAPA Hyper Prep kit ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 2 hours at 25°C after which the circularized RNA was purified with the Oligo Clean & Concentrator kit (D4061) by Zymo Research ...
-
bioRxiv - Genomics 2023Quote: ... at 37 °C for 2 hours and purified using Zymo ssDNA/RNA clean & concentrator kit following manufacturer’s instructions (Zymo D7011). Next ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the 100–400nt region was cut and dissolved in 1X TEN250 buffer (1X TE 7.4, 0.25M NaCl) at 80°C for 30min followed by column purification (Zymo research, R1016). For the HEK293T libraries ...
-
bioRxiv - Microbiology 2023Quote: RNA was isolated from mid-log phase protists grown at normal growth temperature or 12°C using the Direct-zol RNA Miniprep kit (Zymo). RNA libraries were generated using the KAPA Stranded mRNA Sequencing Kit (Roche) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated for 30 min at 37°C and purified using the RNA Clean & Concentrator-5 kit (Zymo Research).
-
bioRxiv - Developmental Biology 2024Quote: ... The DNA was digested with 0.5 mg/ml Proteinase K for 1 hour at 65°C and then purified using the ChIP DNA Clean & Concentrator kit (Zymo) and quantified with QUBIT ...
-
bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were treated with 1 μL of DPNI for 3 h at 37°C and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s protocol (Zymo CN# D4004).
-
bioRxiv - Genetics 2022Quote: ... cells were synchronized in G1 phase by addition of 4 µg/mL α-factor (Zymo research, mating hormone peptide) for 2 h ...
-
bioRxiv - Genomics 2020Quote: ... The 300 bp sized Micro-C library was purified via 1.5 % agarose gel electrophoresis and extracted with Zymoclean Gel DNA Recovery Kit (Zymo Research, D4002) with a final elution volume of 50 μL ...
-
bioRxiv - Developmental Biology 2021Quote: ... The reactions were incubated for 30 min at 37°C and immediately purified using ZYMO RNA Clean & Concentrator (ZYMO RESEARCH, D7011). The purified RNA samples were eluted in 20 μl of nuclease free water ...
-
bioRxiv - Biochemistry 2022Quote: ... These sorted libraries were grown for 1 day at 30°C shaking in SD-CAA media and then 300 µl of the cultures were miniprepped (Zymo Research) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... purified PCR product was digested with EcoRI-HF and BamHI-HF at 37°C for 1.5 hours and purified again using a Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). For the cloning backbone ...
-
bioRxiv - Molecular Biology 2021Quote: ... The reactions were treated with DNAse (15 min at 37°C) and remaining RNA was recovered using Direct-zol RNA mini prep kit (Zymo research) according to the protocol ...
-
bioRxiv - Genetics 2022Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using a YeaStar genomic DNA purification kit (Zymo Research). Equal quantities of DNA from four spontaneous canavanine-resistant clones derived from strain 2299 were combined ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Prepared lysates were stored at -80°C until RNA extraction was performed using the Quick-RNA MiniPrep kit (Zymo Research #R1054), following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2019Quote: ... Digests were heat inactivated for 20 minutes at 80°C then column cleaned using the DNA Clean and Concentrator kit (Zymo Research). Dilute ligations (100-500 ng DNA ...
-
bioRxiv - Biochemistry 2019Quote: ... Beads for protein analysis were incubated with Laemmli buffer and heated at 95°C for 10 min and beads for RNA analysis were exposed to TRI reagent (Zymo Research) for RNA extraction using Direct-Zol (Zymo Research ...
-
bioRxiv - Biochemistry 2019Quote: ... the DNA samples used for 450k analysis were digested overnight at 37°C using BamHI and EcoRI and then bisulfite converted using EZ DNA Methylation-Lightning Kit (Zymo Research) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... The intermediate RNA products were removed with NaOH-EDTA solution in 95 °C for 10 minutes and the single strand DNA oligo probes were purified via DNA clean & concentrator-25 (D4033, Zymo Research). The ssDNA probes were either directly used for Oligopaint FISH or store in aliquots at −80°C.
-
bioRxiv - Cancer Biology 2020Quote: ... Transposition was carried out at 37°C for 30 minutes followed by DNA purification with DNA Clean and Concentrator-25 (Zymo Research) according to manufacturer’s recommendation ...
-
bioRxiv - Biochemistry 2019Quote: ... Elutions were subsequently de-crosslinked overnight at 65 °C and DNA was purified using ChIP DNA Clean and Concentrator (Zymo Research) and DNA was used for qPCR ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... 300µm diameter) follwing a Proteinase K digestion (at 55°C for 2 hours) using a Quick-RNA MicroPrep kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... We performed DNA extractions from two 1.5 ml pellets for each assay-timepoint from our YPD 30°C fitness assays and from four 1.5 ml pellets from our SC 37°C fitness assays using Protocol I from the Yeastar Genomic DNA Kit (Zymo Research), as described previously (Johnson et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... Reactions were incubated for 1 hour at 37 °C and DNA was purified using the DNA Clean & Concentrator-25 kit (Zymo Research) as described by the manufacturer.
-
bioRxiv - Genomics 2021Quote: ... The mixture was incubated at 37°C for 30 minutes and subsequently purified using the ChIP DNA purification kit (Zymo Research) as described by the manufacturer ...
-
bioRxiv - Biochemistry 2022Quote: ... The extracted RNA was treated with DNase I for 15 minutes at 37°C and further purified using a Quick-RNA miniprep kit (Zymo Research) according to the manufacturer’s protocol.
-
bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated for 45 min (NMIA) or 5 min (1M7) at 37 or 60°C following purification using Direct-zol RNA MiniPrep Kit (Zymo Research).
-
bioRxiv - Microbiology 2022Quote: ... were incubated in a total volume of 20 µL for 30 min at 37°C and subsequently column-purified using RNA Clean and concentrator 5 (Zymo Research).
-
bioRxiv - Microbiology 2023Quote: ... The reaction was incubated at 32°C for 4 hours before being treated with DNase for 20 minutes at 37°C then purified using an RNA clean and concentrate kit (Zymo Research). The RNA quality was checked using a MOPS/formaldehyde agarose gel electrophoresis.
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were either resuspended in 30 µL 100% RNALater and stored at -70°C or resuspended in 100 µL TRI reagent (Zymo Research). Samples were crushed with a micropestle and RNA was extracted using a Direct-zol RNA MicroPrep kit (Zymo Research).
-
bioRxiv - Immunology 2023Quote: ... incubated for 15 min at room temperature and either frozen at -80°C or directly followed by RNA isolation using a Direct-zol RNA microprep kit (Zymo Research) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2023Quote: Two hundred and fifty nanograms of linker-ligated genomic DNA were subjected to sodium bisulfite conversion for 210 min at 64°C using the EZ DNA Methylation Kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... The RNAse treatments were carried out for 20 min at 30°C followed by RNA extraction using DirectZolTM (Zymo Research®) kits as per manufacturer’s protocol ...
-
bioRxiv - Genetics 2023Quote: ... and proteinase K (2 h at 65°C) and DNA was extracted with the ChIP DNA Clean & Concentrator kit (Zymo Research). Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB ...
-
Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... The reaction was ended with EDTA for 5 min at 65°C followed by cleanup using the RNA Clean and Concentrator-25 kit (Zymo Research) as described above ...
-
bioRxiv - Cell Biology 2023Quote: ... Bacteria containing the constructs were then grown overnight at 37°C and the DNA was extracted using the Zyppy Plasmid Miniprep Kit (Zymo Research) according to the manufacturer’s protocol.