Labshake search
Citations for Zymo Research :
101 - 150 of 1170 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080; Zymo Research). The kit columns were substituted with Zymo-Spin IC Columns (C1004-50 ...
-
bioRxiv - Neuroscience 2022Quote: ... the resulting solution was incubated with 3 volumes of TRI-reagent at room temperature for 2 hours (R2050-1-50; Zymo Research). The mix was then transferred out of the BSL-3 facilities and nucleic acids were purified using the Direct-zol DNA/RNA miniprep kit (R2080 ...
-
bioRxiv - Genetics 2022Quote: ... cells were synchronized in G1 phase by addition of 4 µg/mL α-factor (Zymo research, mating hormone peptide) for 2 h ...
-
bioRxiv - Plant Biology 2020Quote: Genomic DNA was extracted from aerial parts of 3-week-old seedlings using Quick-DNA Plant/Seed Miniprep Kit (Zymo research, D6020) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: A PCR of all constructs using forward primer pTD2F (5’-TTGGCACCAAAATCAACGGG–3’) and reverse primer SP6 was carried out with SuperFi DNA Polymerase (ThermoFischer Scientific, USA) and agarose gel purified (Zymo Research, USA). These primers amplify the gene insert ...
-
bioRxiv - Zoology 2024Quote: ... Clean-up used a combination of two kits: the beads from the Select-a-size Magbead kit (Zymo Cat# D4084-3-10) and the buffers from Genomic DNA Clean & Concentrator -25 (Zymo Cat# D4064) ...
-
bioRxiv - Microbiology 2023Quote: ... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...
-
bioRxiv - Immunology 2021Quote: ... Samples were incubated at 37°C for 60 minutes and purified using the Zymo DNA Clean and Concentrator-5 Kit (Zymo). Fragments were amplified according to the previously published protocol ...
-
bioRxiv - Synthetic Biology 2023Quote: ... PCR reactions were treated with 1 μL of DPNI for 3 h at 37°C and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s protocol (Zymo CN# D4004).
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated for 30 min at 37°C and purified using the RNA Clean & Concentrator-5 kit (Zymo Research).
-
bioRxiv - Biophysics 2021Quote: ... Samples were then transferred in 4 ml tubes and processed for total RNA extraction with RNA micro kit (ZYMO REAGENT) according to company protocol ...
-
bioRxiv - Cancer Biology 2023Quote: Tumors were then harvested 4 weeks post-injection and processed using Quick-DNA midiprep plus kit (Zymo Research Cat. #D4075). For each processed tumor ...
-
bioRxiv - Cancer Biology 2021Quote: ... genomic DNA and RNA were extracted from fresh frozen tumor (n=2) and normal (n=3) samples using the ZR-Duet DNA/RNA MiniPrep extraction kit (ZYMO RESEARCH, Irvine, CA). Quantification was performed with a NanoDrop One™ spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... the aqueous phase was diluted back in TRizol reagent (1:3) to proceed with RNA purification by zymodirect RNA kit (Zymo research, cat. R2051). The quality of RNA samples was assessed using Agilent 2200 TapeStation (Agilent Technologies ...
-
bioRxiv - Physiology 2020Quote: ... from all the experimental groups (n=3/group) was subjected to bisulphite treatment using the EZ DNA methylation™ kit (Zymo Research, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... one set of replicates (total of 3 replicates per treatment) was harvested for DNA and RNA using ZR-Duet DNA/RNA MiniPrep (Plus) kit (Zymo Research Cat # D7003) following manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... purified PCR product was digested with EcoRI-HF and BamHI-HF at 37°C for 1.5 hours and purified again using a Zymo DNA Clean and Concentrator-5 (Zymo Research D4013). For the cloning backbone ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... We performed DNA extractions from two 1.5 ml pellets for each assay-timepoint from our YPD 30°C fitness assays and from four 1.5 ml pellets from our SC 37°C fitness assays using Protocol I from the Yeastar Genomic DNA Kit (Zymo Research), as described previously (Johnson et al. ...
-
bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated for 45 min (NMIA) or 5 min (1M7) at 37 or 60°C following purification using Direct-zol RNA MiniPrep Kit (Zymo Research).
-
bioRxiv - Microbiology 2022Quote: ... were incubated in a total volume of 20 µL for 30 min at 37°C and subsequently column-purified using RNA Clean and concentrator 5 (Zymo Research).
-
Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... The reaction was ended with EDTA for 5 min at 65°C followed by cleanup using the RNA Clean and Concentrator-25 kit (Zymo Research) as described above ...
-
bioRxiv - Microbiology 2023Quote: ... The mixture was incubated at 37°C for 1 hour and then purified with Zymo DNA Clean & Concentrator-5 (Zymo Research) following the PCR product protocol and eluting DNA into 10.5 μL of water ...
-
bioRxiv - Molecular Biology 2024Quote: ... The resulting RNA pellets were resuspended in water and digested with 2 U of DNAse I for 30 min at 37 °C followed by purification using RNA clean & concentrator 5 columns (Zymo Research). mRNA levels in every fraction was quantified by RT-qPCR as described above ...
-
bioRxiv - Molecular Biology 2024Quote: ... The resulting RNA was digested with 2 U of DNAse I for 30 min at 37 °C and further purified using RNA clean & concentrator 5 columns (Zymo research). About 1.5 μg of total RNA was retrotranscribed with hexamers using Superscript VI retrotranscriptase (Invitrogen) ...
-
bioRxiv - Genomics 2020Quote: ... DNA in the initial 20-μL nucleic acids solution was digested by adding 16-μL DNase I (1 U/μL) plus 4-μL 10X DNase I buffer (DNase I set; Zymo Research), incubating for 15 min at room temperature ...
-
bioRxiv - Genomics 2020Quote: ... 4-S-UTP containing RNA was eluted in 100mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research) with in-column DNAse reaction to eliminate traces of genomic DNA ...
-
bioRxiv - Microbiology 2020Quote: Total RNA was isolated from Vero cells after 4 days post infection using the Direct-zol RNA isolation kit (Zymo Research). 1 μg of RNA isolate was subjected to rRNA removal with the NEBNext rRNA Depletion Kit (NEB ...
-
bioRxiv - Bioengineering 2022Quote: The isolated yeast populations obtained after round 4 were subjected to colony screen and plasmid recovery by using Zymoprep kit (Zymo Research). The plasmids were transformed into E ...
-
bioRxiv - Genomics 2020Quote: ... 4-SUTP containing RNA was eluted in 100 mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research) with in-column DNAseq reaction to eliminate traces of genomic DNA ...
-
bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from rosette tissue harvested at ZT 23 on day 25 post imbibition (n = 4 biological replicates) using the Direct-zol-96 MagBead RNA kit (Zymo Research). RNA was checked for degradation using a Tapestation 4150 (Agilent ...
-
bioRxiv - Microbiology 2024Quote: Genomic DNA was isolated from the fresh feces collected from UT1- or PBS-gavaged mice (female, n = 4/group) using a ZymoBIOMICS DNA Miniprep Kit (Zymo Research). Note ...
-
bioRxiv - Molecular Biology 2024Quote: ... The isolated yeast populations obtained after round 4 were subjected to colony screening and plasmid recovery using a Zymoprep yeast plasmid kit (Zymo Research). The plasmids were transformed into E ...
-
bioRxiv - Microbiology 2020Quote: ... pH 7.2) and 3 swabs (2 for metagenomic DNA isolation, 1 for storage) moistened with DNA/RNA shield (Zymo Research, Cat. No. ZYR.R1100-250). Swabbing was performed for 2 min in each site and stored in respective storage liquids (i.e ...
-
bioRxiv - Plant Biology 2022Quote: ... Then, samples were centrifuged (14000 g, 3 min) and the supernatants were transferred to silica columns to bind DNA (Zymo Research, Irvin, California, USA). After 1 min of incubation ...
-
bioRxiv - Microbiology 2024Quote: ... The column was then washed with 200 μl of RNA wash buffer and subjected to desulfonation by adding 200 μl of RNA desulphonation buffer (Zymo Research #R5001-3-40) to the column ...
-
bioRxiv - Molecular Biology 2024Quote: ... Coprecipitated proteins were released from the beads by heating/denaturation (CBD-, s-AP) in 1 × loading buffer or eluting with Strep-Elution buffer (Strep-AP, Zymo Research, P2004-3-30) or lysis buffer containing 3xFlag peptide (Flag IP ...
-
bioRxiv - Genomics 2024Quote: ... and three susceptible (n= 3) untreated seedlings were used for genomic high molecular weight DNA isolation using the Quick DNA HMW MagBead Kit (Zymo Research, Irvine, CA, USA). DNA integrity ...
-
bioRxiv - Microbiology 2020Quote: ... and 7) ZymoBIOMICS™ DNA Microprep kit (Zymo Research, California, USA) with modifications ...
-
bioRxiv - Microbiology 2020Quote: ... at 37°C for 30 min and concentrated to 8μL using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA). Full protocol for nucleic acid extraction and subsequent cDNA generation is available at https://www.protocols.io/view/sequence-independent-single-primer-amplification-o-bckxiuxn.
-
bioRxiv - Genetics 2022Quote: ... 30 ng of purified dsDNA was tagmented at 55 °C for 15 min and purified using DNA Clean & Concentrator-5 kit (Zymo Research, D4004) The tagmented DNA was PCR amplified using Nextera primers ...
-
bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 1 h and further cleaned up by using a Zymo RNA Clean and Concentrator-5 purification kit (Zymo Research, R1016). All the oligos used in the library preparation are noted in Supplementary Table 6a.
-
bioRxiv - Genomics 2020Quote: DNA was extracted using 4-10 unstained slides of FFPE tissue using the Pinpoint Slide DNA Isolation system (Zymo Research, Orange, CA). In brief ...
-
bioRxiv - Biochemistry 2024Quote: ... at 37 °C for specific time (20 min, 1 hour, 2 hours, 4 hours, or 16 hours) followed by Oligo Clean & Concentrator Kits (OCC, Zymo Research, D4061) cleanup ...
-
bioRxiv - Immunology 2021Quote: MSP1-specific B cells that successfully expanded in culture were collected by centrifugation (5 min. at 250 × g and RT) and stored at -70°C in 50 µl Tri-Reagent (Zymo #R2050-1-200). Heavy and light chain variable regions were amplified from MSP1-specific B cells by cDNA synthesis and a series of PCR reactions ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The cells were then harvested by centrifugation at 3500g for 4 min and prepared for transformation using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA). For plasmid transformations ...
-
bioRxiv - Microbiology 2023Quote: ... control samples (n = 4, one for each extraction batch) using commercially available kits optimized for fecal-based microbial communities (Zymo fecal/soil kit), and some aliquots archived ...
-
bioRxiv - Microbiology 2024Quote: ... DNA was extracted from the PMA-treated soil samples (n = 48) and the no-template (water) controls (n = 4) using the Quick-DNA Fecal/Soil Kit (Zymo Research, Freiburg, Germany), which is designed explicitly for soil-based microbial communities ...
-
bioRxiv - Plant Biology 2024Quote: ... Samples for Figure 7 were cleaned up with RNA Clean & Concentrator (Zymo) prior to quantification and cDNA synthesis.
-
bioRxiv - Cancer Biology 2023Quote: DNA from 32 skull base chordomas and 4 NP samples were bisulfite converted with EZ-96 DNA Methylation kit (Zymo Research, Orange, CA, USA) and used for genome-wide DNA methylation profiling with Methylation EPIC (Illumina ...
-
bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were generated using the Illumina NextSeq 500 High Output Kit v2.5 (Cat. #20024908) and eluted in DNA Elution Buffer (Zymo Research, Cat. #D3004-4-10). Library concentration and average size was verified using the TapeStation DNA 5000 Assay Reagents and Screen Tapes (Agilent ...