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Citations for Zymo Research :
1 - 50 of 1170 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 and 4 by lysing cells in the 6-well plate in RNA lysis buffer (Zymo) and freezing at −80 °C until extraction ...
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bioRxiv - Developmental Biology 2024Quote: ... using the following two primer sequences (T7 Forward: 5’-TAATACGACTCACTATAGCGTCATC-3’; Reverse: 5’-TTGTCGCACGTTCGGTGTCG-3’) and purified with DNA Clean and Concentrator-5 Kit (Zymo Research, 11-302). dsDNA was converted to RNA with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Genomics 2022Quote: ... followed by max speed centrifugation at 4 °C for 5 minutes and DNA purification by column purification (Zymo) prior to sample preparation for next-generation sequencing.
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bioRxiv - Genomics 2020Quote: ... The genomic DNA of JLat cells and CD4 T cells from individuals #3 and #4 were extracted with a Quick-DNA Miniprep Plus kit (Zymo Research #D4068) according to the product protocol ...
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bioRxiv - Immunology 2024Quote: ... and 3×104-5×104 cells were collected for RNA isolation (RNA Clean & Concentrator-5, Zymo # R1013). The isolated RNA was hybridized with a nCounter custom probe set (108 probes ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl RNA from total or immunopurified ribosomes were 3’ dephosphorylated and 5’ phosphorylated as described above 30 and was selectively recovered with RNA Clean and Concentrator-5 (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... into the 20 μl ligation reaction for incubation with 30 minutes at 30°C and 3 minutes at 65°C to inactivate the enzymes followed by column purification (Zymo Research, R1016). Before the reverse transcription (RT) ...
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bioRxiv - Microbiology 2023Quote: ... ∼4-6×109 cells were concentrated and suspended in 500 μl of DNA/RNA Shield (Zymo Research) in 2 mL screw cap tubes ...
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bioRxiv - Biochemistry 2024Quote: ... 4 °C) and flowed over 1.1 mL volume streptactin column (Streptactin XT superflow, Zymo Research, ZRE160702). Flow through was collected and added to the column a second time ...
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bioRxiv - Genetics 2023Quote: ... Microinjections were performed with 4-5 µg/µl (diluted in nuclease free water, Zymo Research) fas5 and GFP dsRNA on a Femtojet microinjector (Eppendorf ...
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bioRxiv - Genetics 2024Quote: ... GMSM-K - 6 non-risk and 7 risk genotype for rs11119348 and 4 each genotype for rs661849) using Quick-DNA/RNA Miniprep kit (Zymo Research #D7001) followed by DNase treatment using manufacturer’s instructions (Thermo Fisher Scientific #AM1907) ...
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bioRxiv - Molecular Biology 2024Quote: ... 4°C) and DNA contained in the supernatant was extracted using the DNA Clean & Concentrator kit (Zymo D4013). The fragment sizes were assessed by Agilent BioAnalyzer to choose the appropriate MNase concentration yielding a 90/10 monomer/dimer ratio.
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bioRxiv - Genomics 2024Quote: ... worms were thawed at 4°C and subsequently preserved in DNA/RNA Shield (Zymo Research, Irvine, CA, USA). Adults of T ...
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bioRxiv - Physiology 2020Quote: Total RNA was extracted from late third instar fat bodies (n = 4-6 pooled/sample) using a Direct-zol RNA MicroPrep kit (Zymo Research). DNAse-treated total RNA (1 µg ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA from 105 days old CorticOs (N = 4; n = 6-8 per genotype) was extracted using the Direct-zol RNA Miniprep Plus kit (Zymo Research) following the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2024Quote: 20 zebrafish embryos per biological replicate were collected at 4 or 6 hpf and snap-frozen in 300 µl of DNA/RNA Shield (#R1100-50, Zymo Research). Total RNA then was extracted using Direct-zol RNA Miniprep Kit (#R2050 ...
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bioRxiv - Neuroscience 2023Quote: OE from 3 controls and 3 sFlt1 mice was dissected and immediately placed in RNA lysis buffer (Zymo Research R1051) without vortexing for 5min and further processed according to manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: ... and stored at -20°C until (≤ 3 days) the DNA was extracted via the Zymo Quick-DNA Fecal/Soil Microbe kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... 4 ml of urine was added to 4 ml of 2X DNA/RNA Shield® (Zymo, USA). Serum and urine samples were stored and transported on ice to the Institute of Microbiology at Universidad San Francisco de Quito and thereafter maintained at −20°C until DNA extraction.
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA from six whole 20-21 dpf offspring (3 control offspring, 3 treatment offspring) was extracted using a Zymo Duet extraction kit (Zymo, NZ). The integrity of RNA samples was determined using an Agilent RNA 6000 Nano chip on an Agilent 2100 Bioanalyzer to check that the samples had an RNA Integrity Number (RIN ...
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bioRxiv - Immunology 2021Quote: ... Samples were stored in RNAlater (Invtrogen) for 24 hours at 4°C and then homogenized in TRI Reagent (Zymo Research) with a beadbeater ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl DNase I (6 U/μl) (Zymo Research) and 75 μl DNA Digestion Buffer (Zymo Research ...
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bioRxiv - Microbiology 2020Quote: ... and mRFP1 was PCR amplified using primers listed in Table 3 and purified using DNA Clean and Concentrator-5 kit (Zymo Research). pUC19 was digested with PstI and pBAD18 was digested with EcoRI and HindIII ...
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bioRxiv - Developmental Biology 2020Quote: RNA was extracted from at least two biological replicates for each sample of cells at days 3 and 5 of differentiation using Direct-zol RNA extraction kit (Zymo Research). mRNA was captured using NEBNext (Poly A ...
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bioRxiv - Cancer Biology 2023Quote: ... change of reverse transcription primers for compatibility with standard Illumina sequencing primers 3) cDNA clean-up prior to library amplification performed with the DNA Clean & Concentrator-5 kit (Zymo, #D4013) 4 ...
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bioRxiv - Genomics 2024Quote: Neutralization Buffer (Zymo Research, cat. no. D4027-3-50)
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bioRxiv - Systems Biology 2023Quote: ... 600 µL DNA/RNA wash buffer (Zymo, #D7010-3), and 400 µL DNA/RNA wash buffer ...
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bioRxiv - Microbiology 2020Quote: ... Samples were centrifuged 1 min at 12000 g at 4°C and RNAs were extracted from supernatants with Direct-zol RNA Mini Prep kit (Zymo Research), following manufacturer’s protocol and including the optional DNAse treatment step ...
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bioRxiv - Bioengineering 2023Quote: ... The lysates were centrifuged at 5000 x g for 10 min at 4 °C and supernatant added to gDNA extraction columns (Zymo Research). The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research ...
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bioRxiv - Genetics 2021Quote: ... we dissected embryonic tails at E12.5 (n = 4) and E14.5 (n = 4) and extracted RNA using 50 µl Direct-zol (Zymo Research) following the manufacturer’s protocol and used the same library preparation procedures as for the parental samples ...
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bioRxiv - Molecular Biology 2021Quote: ... to block the 3’-ends from ligation and were then size-selected to 200 ≥ nt (DNA Clean & Concentrator™-5, Zymo Research). 5’ adaptors were attached using T4 RNA ligase (Ambion ...
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bioRxiv - Genetics 2023Quote: ... Cells were cultured for 3-5 days post-transfection after which genomic DNA was extracted using the Quick-DNA Microprep Kit (Zymo Research Corporation).
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bioRxiv - Physiology 2023Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick- RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
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bioRxiv - Genetics 2023Quote: ... 30 samples were collected (5 timepoints x 2 genotypes x 3 replicates) and RNA was extracted using a Direct-zol RNA Miniprep (Zymo Research R2061) using TRIzol Reagent (ThermoFisher Cat # 15596026 ...
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bioRxiv - Cell Biology 2022Quote: Total RNAs were extracted from BMDMs (male mice: 4 Cre+ and 4 Cre−) using the Quick-RNA miniprep plus kit (Zymo). With a minimum of 300 ng input RNA ...
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bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Genomics 2024Quote: ... 3) human fecal microbiome (ZymoBIOMICS Fecal Reference, Zymo Cat #D6323) and 4 ...
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bioRxiv - Molecular Biology 2023Quote: ... nucleus accumbens and lung of the 4 juvenile and 4 old female mice was bisulfite converted using the EZ DNA methylation Gold kit (Cat# D5006, Zymo Research) according to manufacturer’s instructions and eluted ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mL of suspended biomass was collected in triplicate from the mixed tank and PBR effluent and stored in 5 mL polypropylene transport tubes (FisherbrandTM, USA) prefilled with 3 mL DNA/RNA ShieldTM (Zymo Research corp., CA, USA). The samples were briefly vortexed and stored at -20 °C until they were shipped to University at Buffalo (UB ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction mixture was incubated at 65 °C for 3 h and the RNA was then isolated by using the Zymo RNA clean and concentrator-5 kit (Zymo Research, cat. no. R1013) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... cells collected by centrifugation (6000 xg, 10 min, 4°C) and DNA extracted using the ZR Fungal/Bacterial DNA Kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... the 2h condition was harvested in TRI Reagent (Zymo Research, R2050-1) and the rest of infected-HIE were resuspended in BME and plated in a pre-warmed 24-well plate (3x 10uL drop/condition) ...
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bioRxiv - Systems Biology 2022Quote: ... the second sample was stored at RT for 3 – 5 days and the third sample was collected in a Zymo research tube (Zymo Research Inc., Irvine, CA, USA) containing 9ml DNA/RNA shield and stored at RT for 3 – 5 days before storage at −80 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was hydrolysed for 6 h at 37°C using DNA degradase plus (Zymo Research). Mass spectrometry quantification was performed as previously described36 in the Babraham Institute mass spectrometry facility using three individual E18.5 wild-type and three individual E18.5 Tet3 knockout embryos.
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from ∼3×105 cells using Direct-zol mini kit (Zymo) or Trizol reagent (Invitrogen ...
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bioRxiv - Synthetic Biology 2022Quote: ... The resulting product was purified using 4 spin columns (Zymo, DCC-25) to produce the round 1 product.
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bioRxiv - Pathology 2024Quote: ... and 4 tonsils) with Quick-DNA/RNA FFPE Miniprep Kit (Zymo Research). Barcoded RNA-seq library was established with NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Developmental Biology 2022Quote: ... GATA-3 and RORC were generated by PCR with ZymoTaq PCR Master Mix (Zymo Research) using the primers listed in Table 1 ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted from ∼3×106 cells by using Quick-DNA Kit (Zymo Research) according to manufacturer’s instructions ...