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Citations for Zymo Research :
51 - 100 of 1170 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... After 3 h of growth cells were arrested with 25 μM α-factor (Zymo Research) for 3 h ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted from ∼3×106 cells by using Quick-DNA Kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified from a 3 mL overnight liquid culture of a single colony (Zymo #D4016).
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bioRxiv - Genomics 2023Quote: ... Libraries were eluted in DNA Elution Buffer (Zymo Research, Cat #D3004-4-10). The concentration and average size of the libraries was verified using the TapeStation DNA 5000 Assay Reagents and Screen Tapes (Agilent ...
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bioRxiv - Genomics 2023Quote: ... The DNA pellet was eluted in elution buffer (D3004-4-10 Zymo Research) and incubated at 50°C for 10 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... incubated at 65 °C for 6 hours and extracted with ChIP DNA Clean&Concentrator kit (Zymo Research, USA).
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bioRxiv - Synthetic Biology 2022Quote: ... at a ratio of 1:3 (DNA : Zymo Shield / 20 µl DNA:60 µl Zymo Shield). O volume of 5µl of the PCR product was loaded on a gel for confirmation of the reaction ...
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bioRxiv - Synthetic Biology 2022Quote: ... at a ratio of 1:3 (DNA : Zymo Shield / 20 µl DNA:60 µl Zymo Shield). O volume of 5µl of the PCR product was loaded on a gel for confirmation of the reaction ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from Calu-3 lysates using Direct-zol RNA Miniprep Plus Kit (Zymo Research) and reverse transcribed using High-Capacity cDNA RT kit (Applied Biosystems ...
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bioRxiv - Genetics 2024Quote: CO (n=3) were pooled together for RNA extraction using Quick-RNA Miniprep Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The DNA pellet was eluted in an elution buffer (D3004-4-10 Zymo Research) and incubated at 50°C for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted from 3 x 105 Raji cells using Direct-zolTM RNA MiniPrep kit (Zymo Research) according to manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 aliquots were collected in 1 ml stabilization buffer tubes (DNA/RNA Shield, Zymo Research, Irvine, California) using sterile swabs ...
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bioRxiv - Physiology 2024Quote: ... and KI-TTL mice (N=3 per group) using the Direct-zol RNA Microprep Kit (Zymo Research) followed by a DNase digestion step ...
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bioRxiv - Molecular Biology 2024Quote: ... The band at roughly 3 kb was extracted using a Zymoclean Gel DNA Recovery Kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Pellets were then resuspended in Zymo DNA elution buffer (Zymo, Cat No: D3004-4-10), and placed on the heat block at 65°C for 1 hour to completely dissolve the genomic DNA.
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bioRxiv - Genomics 2024Quote: ... Pellets were then resuspended in Zymo DNA elution buffer (Zymo, Cat No: D3004-4-10), and then incubated at 65°C for 1 hour to completely dissolve the genomic DNA.
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bioRxiv - Bioengineering 2021Quote: Genomic DNA was extracted from 3×106 HEK293_LPCD19/BiTE cells using Quick-DNA midiprep kit (#D4075, Zymo Research). Gene specific primers annealing to the signal sequence (5’-CTGGTCCTGCATCATCCTGTTTC-3’ ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... We then lysed 3 dpf larvae individually by rigorously pipetting in 55 μl DNA/RNA Shield (Zymo Research) at RT followed by overnight incubation at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and were filled with 3 µl of the RNA Lysis Buffer (Quick-RNA Microprep Kit, Zymo, Irvine, Ca). The pipette was mounted on a micromanipulator and used to aspirate 2– 10 corpuscles from duck bill skin by applying negative pressure using a high-speed pressure clamp system (HSPC-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Infectious viral stocks were inactivated by a 1:3 dilution with TRI Reagent® (Zymo, R2050-1-200) immediately prior to RNA extraction ...
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bioRxiv - Plant Biology 2024Quote: Genomic DNA was extracted from 3-week-old seedlings with Quick-DNA Plant/Seed Miniprep Kit (ZYMO, D6020). 200ng fragmented genomic DNA (200bp-500bp ...
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bioRxiv - Molecular Biology 2023Quote: ... cfRNAs were then purified and concentrated into 6 µl using an RNA Clean and Concentrator-5 kit (Zymo, R1016). Plasma extracellular vesicles (EVs ...
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bioRxiv - Developmental Biology 2024Quote: ... total RNA was isolated at 4 hpf using Direct-zol RNA Miniprep Kit (#R2050, Zymo Research) following manufacturer’s instructions and quantified using the Qubit fluorometric quantification (#Q10210 ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA products were separated on a 3% agarose gel and then extracted using gel extraction kit (ZYMO research). In vitro transcription with T7 RNA polymerase and in vitro Dicer cleavage assays were performed as previously described (Sheng et al ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from 3×105 cells per replicate using Direct-Zol RNA Miniprep Kit (Zymo Research, USA) according to the manufacturer’s protocol and shipped in dry ice to the MDC/BIH Genomic Core Facility for quality assessment using TapeStation (Agilent Technologies) ...
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bioRxiv - Systems Biology 2023Quote: ... 3 μl of reaction mix were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Bioengineering 2024Quote: Cell number was directly assessed as a function of total isolated DNA at experimental Days 1 and 7 using a Zymo Quick-DNA Microprep Plus Kit (n=5, Zymo Research Crop, Irvine, CA). A standard curve at experimental Day 0 was generated using known quantities of hMSCs and following the “Biological Fluids & Cells” protocol as directed ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated at 37 °C for 30min and column purified using the RNA Clean and Concentrator-5 (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... according to the manufacturer’s protocol (for more than 4×106 cells) or Quick-RNA MicroPrep kit (Zymo research R1051 ...
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bioRxiv - Genomics 2020Quote: ... the total RNA was extracted from active and tun samples (3 replicates) using Direct-Zol RNA MicroPrep Kits (Zymo Research), and the sequencing library was constructed using SMARTer v4 Ultra Low Input RNA Kit for Sequencing (Clonetech ...
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bioRxiv - Genetics 2022Quote: Yeast colonies containing bait and prey constructs that grew on Trp−/Leu−/His− + 25 mM 3-Amino-1,2,4-triazole (3AT) selection plates were extracted using the Zymoprep Yeast Plasmid Miniprep II (Zymo Research, D2004) followed by Sanger sequencing for gene identification ...
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bioRxiv - Plant Biology 2023Quote: RNA was extracted from 3 biological replicates of 100mg of frozen tissue of each accession using the Quick-DNA/RNA Miniprep kit (Zymo) following manufacturer’s instructions with the following modifications ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: DNA libraries for Nanopore sequencing were prepared by extracting plasmid DNA from ~1 × 109 cells (input library, 30 ml culture) or ~3 × 107 cells (output populations, 1 ml culture) using Zymoprep Yeast Plasmid Miniprep (Zymo). One freeze-thaw cycle (at −80°C ...
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bioRxiv - Genomics 2023Quote: ... 3 colonies were randomly selected and further expanded for plasmid DNA purification (Zyppy™ Plasmid Purification Kit; Zymo research #D4019). Extracted DNA was then sequenced to verify the integrity of the cloned fragments.
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from a single leaf of 3-week-old T4 Setaria lines using the Directzol RNA kit (Zymo) including on-column DNAse treatment ...
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bioRxiv - Developmental Biology 2024Quote: ... at 37°C for 30 minutes and treated with the RNA Clean and Concentrator-5 kit (Zymo Research). The purity and concentration of the resulting RNA were assessed using Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... at 37°C for 30 minutes and treated with the RNA Clean and Concentrator-5 kit (Zymo Research). A total of 1 µg of RNA was used as a template for cDNA synthesis using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV RT ...
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bioRxiv - Molecular Biology 2023Quote: ... for 30 min at 37 °C and column-purified using the RNA Clean & Concentrator-5 kit (Zymo Research), eluting in 12.5 μL RNase-free water ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for 30 minutes at 37 °C and products were further cleaned with RNA clean & concentrator 5 (Zymo Research) and eluted with 8-10 µl RNase free water ...
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bioRxiv - Evolutionary Biology 2024Quote: ... for 1h at 37°C and then purified with the Zymo DNA Clean and Concentrator-5 kit (Zymo). Illumina sequencing adaptors and barcodes were added to the DNA fragments ...
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bioRxiv - Microbiology 2024Quote: ... hydrophila MAH-4 was pelleted and genomic DNA obtained using Zymo DNA Miniprep (Zymo Research, Irvine, CA, USA). Long reads and short paired end reads (2 x 151 bps ...
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bioRxiv - Genetics 2023Quote: ... individual array bearing worms were isolated and lysed in 4µl of EB (Zymo, Cat. No.: D3004-4-16) buffer with 1mg/ml proteinase K (NEB) ...
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bioRxiv - Genetics 2023Quote: ... we extracted DNA from 2–4 dpf whole larvae using the Quick-DNA Microprep Kit (D3021, Zymo Research). We then performed a PCR for GFP as well as a ‘junction PCR’ to amplify a region including both mitfa and GFP to ensure integration at the intended locus.
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bioRxiv - Cancer Biology 2022Quote: ... Individual 50 μL reactions were run on a 3% agarose gel and libraries were purified using the Zymoclean Gel DNA Recovery kit (D4007, Zymo Research). Sequencing was performed using standard Illumina instructions ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 10 cm plates of SKMEL5 cells with 3 biological replicates per condition (DMSO, 100 nM binimetinib, 1 μM binimetinib) using Direct-zol RNA miniprep kit (Zymo Research), as previously described (24) ...
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bioRxiv - Genomics 2021Quote: ... The nascent RNA was purified by adding 3 volumes of TRIzol and processed with the Direct-zol RNA Miniprep kit (Zymo Research) with addition of 1/100 volume of 100 mM DTT to each supplied wash buffer.
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bioRxiv - Microbiology 2021Quote: ... and genomic DNA was extracted from 3 mL of overnight (TSB) broth culture using the ZR Duet DNA/RNA MiniPrep Plus kit (Zymo Research) following manufacturer’s protocol for the “suspended cells” option ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
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bioRxiv - Microbiology 2022Quote: ... The high molecular weight bands were excised with a razor and dissolved in 3 volumes of Zymo Agarose Dissolving Buffer (Zymo D4001) at 50°C ...