Labshake search
Citations for Zymo Research :
1251 - 1300 of 1309 citations for Genz 644282 CAS 529488 28 6 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: The soil microbiome DNA was extracted using 200 mg of soil from each pot and the Zymo Quick-DNA™ Fecal/Soil Microbe Miniprep Kit (Zymo Research, Irvine, CA). DNA purity was assessed using spectrophotometry (NanoDrop model ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from fixed and sorted βHI or βLO using the Zymo Research Quick-DNA Microprep Plus Kit (Zymo Research, Irvine, CA USA), according to manifacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was also performed on three no-sample added (negative) and three ZymoBIOMICS Microbial Community Standard (positive) controls (Zymo Research, Irvine, CA, USA). DNA from an additional eight GWSS had been previously extracted using the Blood and Tissue kit (Qiagen ...
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bioRxiv - Genomics 2023Quote: ... Three DNA libraries from the same DNA extraction were prepared with further purification and concentration using the Genomic DNA Clean and Concentrator kit (Zymo Research, Irvine, CA, USA). The DNA was then selected for high molecular weight DNA using the PacBio Short Read Eliminator kit (PacBio ...
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bioRxiv - Microbiology 2023Quote: ... Pith samples had low concentrations of DNA and were subsequently cleaned and concentrated into 35 µl using the Zymo Clean & Concentrate-25 kit (Zymo Research Corporation, Ca, USA). For all samples ...
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bioRxiv - Developmental Biology 2023Quote: ... Whole placentas were equilaterally quartered and homogenized in RNA Lysis buffer and RNA was extracted using the Zymo QuickRNA miniprep kit (Zymo Research, Irvine, CA USA) according to manufacturers recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Large-scale DNA plasmid was amplified in Escherichia coli DH5α and purified by using a plasmid giga prep kit (Zymo Research, Costa Mesa, CA). DNA sequencing was performed to confirm the plasmid sequence’s authenticity.
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bioRxiv - Neuroscience 2023Quote: DNA from each pool of 5-HT+ or 5-HT-cells was isolated and bisulfite-converted using the EZ DNA Methylation-Direct Kit (Zymo Research, Irvine, CA, USA) with the following adaptations ...
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bioRxiv - Microbiology 2023Quote: vIL-6/THP-1 and parental THP-1 cells were infected with or without r219.KSHV for 72h and RNA was purified using the Quick-RNA miniprep kit (Zymo Research, Irvine, CA, USA). Indexed ...
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bioRxiv - Microbiology 2023Quote: ... we then tested the fidelity of this kit by extracting DNA from gypsum spiked with cells from a mock community (ZymoBIOMICS Microbial Community Standard, Zymo Research, Irvine, CA, USA). We added 25 μL of this standard (∼4.7×108 cells ...
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bioRxiv - Bioengineering 2024Quote: ... 1 mL of suspended biomass was collected in triplicate from the mixed tank and PBR effluent and stored in 5 mL polypropylene transport tubes (FisherbrandTM, USA) prefilled with 3 mL DNA/RNA ShieldTM (Zymo Research corp., CA, USA). The samples were briefly vortexed and stored at -20 °C until they were shipped to University at Buffalo (UB ...
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bioRxiv - Microbiology 2024Quote: ... total RNA was extracted using the Direct-zolTM RNA MiniPrep kit according to the instructions of the manufacturer (Zymo Research Corp, Irvine, CA, USA). DNA was digested by adding 8 µl of 10X DNase buffer ...
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bioRxiv - Zoology 2024Quote: ... These amplified products were then purified as above and restriction enzyme digested CCHa1R and CCHa2R sequences were ligated into pcDNA3.1+ and pBudCE4.1 mammalian expression vectors followed by bacterial transformation and plasmid DNA purification to obtain a large quantity of receptor constructs using ZymoPURE II Plasmid Midiprep Kit (Zymo Research, Tustin, CA, USA) following the manufacturer recommendation ...
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bioRxiv - Genetics 2024Quote: ... RNA samples were then processed with either Sureprep RNA Cleanup and Concentration Kit (Fisher BioReagents, Fair Lawn, NJ, USA) or RNA Clean and Concentrator (Zymo Research, Irvine, CA, USA). 200ng RNA was reverse transcribed using QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... 750 µl of lysis solution (ZymoBiomics Lysis Solution) and 19 µl 20 mg/ml proteinase K solution in storage buffer (Zymo Research, Irvine, CA, USA) were added into each tube and mixed ...
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bioRxiv - Microbiology 2024Quote: ... total RNA was extracted using the Direct-zolTM RNA MiniPrep kit according to the instructions of the manufacturer (Zymo Research Corp, Irvine, CA, USA). DNA was digested by adding 8 µl of 10X DNase buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA was prepared using the ZymoPure II Plasmid Midiprep Kit followed by an ethanol precipitation and linear expression templates were prepared by PCR and subsequent cleanup using the Zymo DNA Clean and Concentrator (Zymo Research, Irvine, CA, USA).
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from the third eyelid of dromedary C3 with Zymo® according to the manufacturer’s instructions (Zymo Research, Irvine, CA, USA). RNA was quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Genomics 2022Quote: ... we co-extracted DNA and RNA in parallel using a modified version of the Quick-DNA/RNA Microprep Plus Kit (Zymo Research; Irvine; CA U.S.A.). DNA and RNA library prep and sequencing were performed by a service provider (Génome Québec ...
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bioRxiv - Genomics 2022Quote: ... Mosquitoes collected in La Reunion Island were immediately processed in pools of 10 females and homogenized in DNA/RNA-shield (Zymo Research, Irvine CA, USA) with a Kimble electric pestle (DWK Life Sciences ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from three replicates of the spiked and test samples using the Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: We extracted total RNA from snap-frozen testicular tissues from all biopsies using the Direct-zol™ RNA Microprep kit (Zymo Research, CA, USA) according to manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... DNA was eluted in 60 μl elution buffer and further purified by using the OneStep PCR Inhibitor Removal Kit (ZymoBIOMICS, Zymo Research, Irvine, CA, USA).
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bioRxiv - Cancer Biology 2023Quote: DNA from 32 skull base chordomas and 4 NP samples were bisulfite converted with EZ-96 DNA Methylation kit (Zymo Research, Orange, CA, USA) and used for genome-wide DNA methylation profiling with Methylation EPIC (Illumina ...
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bioRxiv - Plant Biology 2024Quote: The DNA was extracted from 250 mg of soil using the Zymo Quick-DNA™ Fecal/Soil Microbe Miniprep Kit (Zymo Research, Irvine, CA). DNA purity was assessed using spectrophotometry (NanoDrop model ...
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bioRxiv - Genomics 2024Quote: ... DNA and RNA were extracted from wastewater samples were extracted using the Zymo Environ Water RNA Kit (Cat No. R2042, Zymo Research Corp, Irvine, CA). Briefly ...
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bioRxiv - Physiology 2024Quote: ... Complementary DNA (cDNA) was synthesized from 1 to 2 μg of RNA using High-Capacity RNA-to-cDNA™ Kit (Zymo Research, Irvine, CA). Quantitative real-time polymerase chain reaction (qPCR ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted directly from the TRI reagent using a Direct-Zol RNA Mini-Prep Kit according to manufacturer instructions (Zymo Research Corporation, Irvine, CA). RNA from all four groups was processed using a Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) ...
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bioRxiv - Cancer Biology 2024Quote: ... mRNA was isolated from the cyclin A/B RxL inhibitor (CIRc-004) and enantiomer control (CIRc-005) treated arms using Quick-RNA™ Miniprep kit (Zymo Research, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The aqueous layer of the phase separation was applied to the column of the Direct-Zol RNA Mini Kit (Zymo; Irvine, CA; Cat #: ZR2070), and isolated following manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Collections from multiple donors were pooled and homogenized in one large suspension together with the microbiome preservative reagent DNA/RNA Shield™ (Zymo Research – CA, USA) to prevent outgrowths or depletions of microbial taxa ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from 100 mg of frozen leaf tissue per sample using either Direct-zol® RNA Purification Kit or Quick-RNATM Miniprep Kit (Zymo Research, CA, USA), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... fecal samples with the ZymoBIOMICSTM DNA Miniprep Kit and the Quick-DNATM Fecal/Soil Microbe DNA MiniPrep Kit (cat. D4300 & D6010, respectively; Zymo Research, Irvine, CA, USA), in accordance with their respective protocols ...
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bioRxiv - Biochemistry 2024Quote: ... in fresh SD-CAA media at 30 °C with shacking (250 rpm) and the DNA plasmid pools extracted using Zymoprep Yeast Plasmid Miniprep II kit following manufacture instructions (Zymo Research, Irvine, CA, USA). Purified yeast-extracted DNA plasmid was then used as template to generate amplicons through two consecutive PCR reactions ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA plasmid present within the yeast cells was extracted using Zymoprep Yeast Plasmid Miniprep II kit following manufacture instructions (Zymo Research, Irvine, CA, USA).22 Purified yeast-extracted DNA plasmid was then used to transform competent E ...
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bioRxiv - Biochemistry 2024Quote: ... All DNA constructs encoding single macrocyclic peptide ligand were verified by Sanger sequencing and further used to transform new competent EBY100 cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA, USA).
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bioRxiv - Biochemistry 2024Quote: ... in fresh SD-CAA media at 30 °C with shacking (250 rpm) and the DNA plasmid pools extracted using Zymoprep Yeast Plasmid Miniprep II kit following manufacture instructions (Zymo Research, Irvine, CA, USA).22 Amplicons for next generation sequencing (NGS ...
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bioRxiv - Bioengineering 2024Quote: Cell number was directly assessed as a function of total isolated DNA at experimental Days 1 and 7 using a Zymo Quick-DNA Microprep Plus Kit (n=5, Zymo Research Crop, Irvine, CA). A standard curve at experimental Day 0 was generated using known quantities of hMSCs and following the “Biological Fluids & Cells” protocol as directed ...
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bioRxiv - Genomics 2024Quote: ... and three susceptible (n= 3) untreated seedlings were used for genomic high molecular weight DNA isolation using the Quick DNA HMW MagBead Kit (Zymo Research, Irvine, CA, USA). DNA integrity ...
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bioRxiv - Microbiology 2020Quote: ... The final pool volume was reduced to concentrate the sequencing library using the DNA Clean and Concentrator™-5 kit (Zymo Research, Irvine, CA, USA). The pooled library concentration was determined using the Quant-iT™ High-Sensitivity DNA Assay Kit (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: The PCR products were checked on an agarose gel stained with ethidium bromide before gel extraction (Zymoclean™ Gel DNA Recovery Kit, Zymo Research, Irvine, CA, USA). The resulting pure amplicons were ligated into pCR™-Blunt II-TOPO® (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... in the vagina with the other hand and rotate it for 10-15 seconds before placing the swab into the provided collection tube (FluidX tubes; 65-7534 - Brooks Life Sciences, Chelmsford, MA, USA; containing 0.8 ml DNA/RNA-shield; R1100-250, Zymo Research, Irvine, CA, USA) and breaking off the handle ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was then transferred to a new tube and further processed on columns using the Direct-zol™ Kit (Zymo Research, Irvine, CA, USA). RNA was treated in-column with DNAse and eluted in nuclease-free water before being stored at −80°C ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from the filtered membranes and from 150–200mg of the nonhuman fecal samples using the ZR Fecal DNA MiniPrep™ Kit (Zymo Research, Irvine, CA, USA), as previously described (Kongprajug et al. ...
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bioRxiv - Microbiology 2023Quote: ... and using sterile forceps placed in ZR-96 BashingBead™ lysis tubes containing 750 μl DNA/RNA Shield™ (Zymo Research Corp., Irvine, CA, USA). The samples were shaken and placed on ice until they returned to the vehicle ...
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bioRxiv - Plant Biology 2023Quote: The expression vectors were introduced into the yeast PichiaPink™ Strain 2 using Frozen-EZ Yeast Transformation II™ (Zymo Research Co., Irvine, CA, USA). Recombinant strains were initially cultured in BMGY liquid medium at 30 °C for about 24 h ...
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bioRxiv - Plant Biology 2023Quote: The bisulfite conversion of sonicated genomic DNA fragments was carried out based on the instructions provided in the EZ DNA-methylation lightning Kit (Zymo Research Corp., Irvine, CA, USA). The libraries were made using the Accel-NGS Methyl-Seq DNA library kit (SWIFT Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... The parasites were washed twice with PBS prior to RNA extraction and purification using the Quick-RNATM MiniPrep kit (Zymo Research, Irvine, CA, United States) according to manufacturer specifications ...
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bioRxiv - Microbiology 2023Quote: DNA extraction was performed from 4g of frozen bedding samples (-80°) or from teat skin swabs with the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA) according to manufacturer’s instructions ...