Labshake search
Citations for Zymo Research :
1151 - 1200 of 1309 citations for Genz 644282 CAS 529488 28 6 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Complete rabies virus nucleoprotein and glycoprotein gene sequences were generated from rabies virus RNA extracted using Direct-zol RNA MiniPrep kit (R2052 Zymo, Irvine, CA, USA). Complete nucleoprotein and glycoprotein genes were amplified using Takara long amplicon Taq polymerase with GC buffers (RR02AG Takara Bio USA ...
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bioRxiv - Immunology 2020Quote: ... The brush is removed from the channel of the bronchoscope and the brush is placed into 200 μL of DNA/RNA Shield X1 (Cat.# R1200, Zymo Research, Irvine, CA). The bronchoscope remains in place while two aliquots of 20 ml of saline each are instilled into the bronchus and subsequently aspirated ...
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bioRxiv - Microbiology 2020Quote: ... isolates were grown shaking in SG broth at 37°C and genomic DNA isolated using the Quick-DNA Fungal/Bacterial miniprep kit (Zymo Research, Irvine, CA) according to the manufacturer's suggested protocol.
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bioRxiv - Microbiology 2020Quote: ... supernatants were collected and viral RNA in the cell supernatants were extracted by using Direct-zol RNA MiniPrep kit (Zymo research, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... DNA was extracted from the resuspended pellet using the Zymo Quick-DNA Microprep Kit (cell suspensions protocol) per manufacturer’s instructions (Zymo Research, Irvine, CA, USA). The DNA was quantified using the dsDNA kit on a Qubit 2.0 (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... The cell pellets were washed with PBS before total RNA was extracted with the Direct-zol RNA Micro-Prep method (Cat. R2063-A, Zymo Research Corp., CA), and the extracted RNA was reverse transcribed using LunaScript® RT SuperMix Kit (Cat ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from the solid and liquid aliquots using the Qiagen AllPrep PowerViral DNA/RNA kit and further purified using the Zymo OneStep PCR inhibitor removal columns (Zymo Research, Irvine, CA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μL of each reaction product were pooled and purified using the Zymo DNA Clean and Concentrator kit (Zymo Research, Irvine, CA). Sequencing was performed at the Chan Zuckerberg BioHub facility on an Illumina NextSeq 550 platform in High Output mode.
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bioRxiv - Developmental Biology 2023Quote: ... Plasmid and fosmid DNA was purified with either the Zyppy Plasmid Miniprep kit or ZymoPURE II Plasmid Midiprep kit (Zymo Research, Irvine, CA). Following restriction digests or PCR ...
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bioRxiv - Genomics 2023Quote: ... Total RNA of the parents and two bulks was isolated using the Direct-ZolTM RNA Kits (Zymo Research Co., Ltd., Irvine, CA, USA). RNA quality was determined using a NanoDrop OneC spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Total RNA was isolated from polarized murine bone marrow-derived macrophages using the Quick-RNA™ MiniPrep Kit (Zymo Research, Irvine, CA, USA). RNA quantity and quality was verified by two methods ...
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bioRxiv - Physiology 2022Quote: ... Samples thawed and then disrupted with an eppendorph tube pestle and total RNA purified using a Direct-zol plus RNA miniprep kit (Zymo Research, Irvine, CA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: HepG2 cell samples were collected into QIAzol Lysis Reagent and total RNA was extracted using Direct-zol RNA Miniprep (Zymo Research, CA, USA). Liver samples were first homogenized in QIAzol Lysis Reagent (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... 2016) Genomic DNA (500ng) from whole blood underwent bisulfite treatment with the Zymo EZ DNA Methylation kit (Zymo Research Corp, Irvine, CA, USA), and 4 µl of bisulfite-converted DNA was measured on the Illumina 450k array following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and urine samples underwent RNA extraction in the Virology Unit at the Institut Pasteur de Madagascar (IPM) using the Zymo Quick DNA/RNA Microprep Plus Kit (Zymo Research, Irvine, CA). In total ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA (500ll!ng) from whole blood was bisulfite-treated using the ZymoResearch EZ DNA Methylation kit (Zymo Research Corp, Irvine, CA, USA), following the standard protocol for Illumina 450K micro-arrays ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH5α λpir (Table 1) was prepared using the commercially available Mix and Go™ transformation kit (Zymo Research International, Irvin, CA, USA) according to the manufacturer’s indications ...
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bioRxiv - Neuroscience 2024Quote: ... A negative control with the control from DNA extraction and a positive control of ZymoBIOMICS™ Microbial Community Standard (ZYMO Research, Irvine, CA) were set during amplicon library preparation ...
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bioRxiv - Microbiology 2023Quote: ... patient wound slough was removed from the ulcer base with a dermal curette and immediately stored in RNA Shield (Zymo Research, Irvine, CA) at 4°C for 24 hours before being frozen at –80°C until further processing ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from approximately 3.0 × 105 EndoC-βH1 using Direct-zol RNA Miniprep Kit (cat. R202-Zymo Research, Irvine, CA, US) following manufacturer’s instructions.
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bioRxiv - Zoology 2023Quote: ... using the manufacturer’s instructions or co-extraction of DNA and RNA was accomplished with a Quick DNA/RNA MiniPrep Plus Kit (Zymo Research, Irvine, CA). The quality and quantity of DNA and RNA were analyzed by either NanoDrop 1000 or NanoDrop One (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... Five hundred nanograms of DNA per sample were bisulfite-treated using the Zymo EZ-96 DNA methylation kit (Zymo Research, Orange, CA, USA). A bisulfite sequencing PCR approach was applied to the promoters of 12 candidate genes (Supplementary Table S3) ...
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bioRxiv - Bioengineering 2024Quote: ... Primers were custom designed by Zymo Research to ensure best coverage of the 16S gene and high sensitivity - Quick-16S™ Primer Set V1-V3 (Zymo Research, Irvine, CA). The final PCR products were quantified with qPCR fluorescence readings and pooled together based on equal molarity ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation was performed according to manufacturer’s protocol using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA, USA, #D6400). The pooled libraries were sequenced on an Illumina MiSeq using MiSeq®Reagent Kit v2 (500-cycle ...
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bioRxiv - Neuroscience 2024Quote: ... Larval and adult samples were completely homogenized in RNA Lysis buffer from the Quick-RNA miniprep Kit (R1055, Zymo Research, Irvine, CA, USA) using a pestle and micro-tube homogenizer (for 30 sec ...
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bioRxiv - Microbiology 2022Quote: ... pisum LSR1 RNA was extracted from a single aphid using the Zymo Quick RNA Miniprep Kit according to the manufacturer protocols (Zymo Research, CA, USA). The RNA was then reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: Up to 75 ng of sheared gDNA was subjected to bisulfite conversion using the EZ-96 DNA Methylation Kit (Zymo Research; Irvine, CA), with liquid handling on a Hamilton MicroLab STAR (Hamilton ...
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bioRxiv - Genomics 2022Quote: ... Genomic DNA (500□ng) from whole blood was bisulfite treated using the Zymo EZ DNA Methylation kit (Zymo Research Corp, Irvine, CA, USA), and 4□μl of bisulfite- converted DNA was measured on the Illumina 450k array[14] following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and an aliquot of 500□ng of genomic DNA was treated with sodium bisulfite using the EZ DNA Methylation Kit according to the manufacturer’s instructions (Zymo Research, Irvine, CA, USA). The Infinium Methylation EPIC BeadChip technology (Illumina ...
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bioRxiv - Genomics 2022Quote: ... 750 ng of DNA was extracted from whole blood and bisulfite converted using the EZ DNA Methylation kit (Zymo Research, Irvine, CA, USA). Methylation was measured from 160 ng of bisulfite-converted DNA using the Illumina EPIC Beadchip (Illumina Inc ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from 50-100 mg seedlings using the Quick-RNA Miniprep Kit with on-column DNase I digestion (Zymo Research, Irvine, CA). cDNA was synthesized with 2–2.5 μg total RNA using the Invitrogen SuperScript III Reverse Transcriptase and the Oligo(dT)20 primer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... was cleaned and concentrated with either sparQ PureMag beads (QuantaBio, Beverly, MA) or the Genomic DNA Clean & Concentrator-10 Kit (Zymo Research, Irvine, CA), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast cells transformed with plasmids encoding the CDM library or control variant are harvested and the plasmid DNA extracted using the Zymoprep Yeast Plasmid Miniprep I Kit (Zymo Research, Irvine, CA). DNA sequences of the variant library are then PCR-amplified using primers with NGS adapters and gel-extracted from the reaction mixture by length of the PCR product ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were then harvested by centrifugation at 3500g for 4 min and prepared for transformation using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA). For plasmid transformations ...
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bioRxiv - Microbiology 2022Quote: ... 2 extracted with tissue samples and 1 run with water samples) and 2 samples of DNA from mock bacterial communities (ZymoBIOMICS Microbial Community DNA Standard, Zymo Research, Irvine CA) were prepared and sequenced ...
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bioRxiv - Microbiology 2022Quote: ... samples were processed for RNA extraction and purification using the Zymo Direct-zol™ RNA Mini Prep kit (Zymo Research, Irvine, CA, USA) per manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were separated by electrophoresis on a 1% agarose gel and purified using the Zymoclean Gel DNA recovery Kit (Zymo Research, Irvine, CA). The purified DNA fragments were then submitted for Sanger sequencing (GENEWIZ ...
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bioRxiv - Systems Biology 2022Quote: ... The renal tubules were washed in dishes containing ice-cold DPBS by pipetting to remove contaminants before RNA extraction using a Direct-zol RNA MicroPrep kit (Zymo Research, Irvine, CA). Four to eight tubules were collected for each sample for a total length of 2 – 4 mm.
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bioRxiv - Plant Biology 2022Quote: ... The sheared DNA was cleaned up and recovered by 1.2x AMPure XP beads followed one round of sodium bisulfite conversion using the EZ DNA Methylation-Lightning Kit (Zymo Research Corporation, Irvine, CA) as outlined in the manufacturer’s instruction with 80 min of conversion time ...
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bioRxiv - Genomics 2022Quote: ... Nucleic acid was isolated with either the Quick Microprep Plus kit (Catalog D7005) or the Zymo Quick Miniprep Plus kit (Catalog D7003) (Zymo Research, Irvine, CA). Gel electrophoresis was performed as described above ...
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bioRxiv - Cancer Biology 2022Quote: ... The outer PCR products amplified using primer #1 and #2 were purified and concentrated using the DNA Clean and Concentrator 5 kit (Zymo Research, Orange, CA) and eluted in 20ul of TE buffer ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 50 mg of soybean leaf tissue using the Direct-zol RNA Miniprep Plus Kit with DNaseI treatment (Zymo Research, Irvine, CA). RNA samples were quantified using a Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... 750 ng of DNA was extracted from whole blood and bisulfite converted using the EZ DNA Methylation kit (Zymo Research, Irvine, CA, USA). Methylation was measured from 160ng of bisulfite-converted DNA using the Illumina EPIC Beadchip (Illumina Inc ...
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bioRxiv - Genomics 2024Quote: ... 250 ng of DNA was extracted from muscle tissue and bisulfite converted using the EZ DNA Methylation kit (Zymo Research, Irvine, CA, USA). Methylation was measured from 250 ng of bisulfite-converted DNA using the Illumina EPIC Beadchip (Illumina Inc ...
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bioRxiv - Genomics 2024Quote: ... 250 ng of DNA was extracted from adipose tissue and bisulfite converted using the EZ DNA Methylation kit (Zymo Research, Irvine, CA, USA). Methylation was measured from 250 ng of bisulfite-converted DNA using the Illumina EPIC Beadchip (Illumina Inc ...
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bioRxiv - Plant Biology 2024Quote: Each composite sample was placed in a 2 ml ZR BashingBead Lysis tube containing beads and ZR stabilization reagent (Zymo Research, Irvine, CA), and was mixed immediately in the field using a bead-beating TerraLyzer lysis system (Zymo Research ...
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bioRxiv - Systems Biology 2024Quote: ... Microbial genomic DNA was extracted from frozen feces (∼100 mg, or 5 pellets) with the ZymoBIOMICS 96 MagBead DNA Kit with lysis tubes (Zymo Research, Irvine, CA), according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and DNA/RNA hybrids were further purified following DNase digestion and additional purification using Zymo-SpinTM columns (ZYMO-RESEARCH CORP, Irvine, CA, USA). Extracts were treated with RNase (to remove all RNAs) ...
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bioRxiv - Microbiology 2024Quote: Libraries for mRNA sequencing were constructed from total RNA sample using the Zymo-Seq RiboFree Total RNA Library Prep Kit (Zymo Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: For DNA extraction cells were collected via filtration and filters transferred to 5 ml cryo vials containing 1 ml DNA/RNA shield (Zymo Research, Irvine, CA) as preservative ...