Labshake search
Citations for Zymo Research :
1001 - 1050 of 1309 citations for Genz 644282 CAS 529488 28 6 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... S4) and cells were collected by filtration for RNA purification using the RNA Clean and Concentrator kit (Zymo Research, Irvine CA). rRNA depletion was accomplished with the Ribo-Zero rRNA Removal Kit (Epicentre ...
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bioRxiv - Biochemistry 2022Quote: ... which were then grown in liquid culture overnight and purified using the ZymoPure endotoxin-free midiprep kit (Zymo Research, Irvine, CA). Transient expression of individual variants for western blots was carried out using a distinct set of expression vectors encoding the untagged CFTR variant cDNAs in the context of a pCDNA5 expression vector ...
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bioRxiv - Cancer Biology 2022Quote: One microgram of genomic DNA was treated with sodium bisulfite using the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... was added to extract total RNA using the Direct-zol RNA Mini Prep Plus kit (Zymo Research, Irvine, CA, United States). The integrity and quality of extracted RNA were checked using an Experion electrophoresis system (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: The samples were processed and analyzed with the ZymoBIOMICS®Shotgun Metagenomic Sequencing Service for Microbiome Analysis (Zymo Research, Irvine, CA).
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bioRxiv - Plant Biology 2022Quote: ... DNA extracted from NIa-N and PR leaves were treated with bisulfite (EZ DNA methylation gold, Zymo Research, Orange, CA, USA) before PCR amplification of the 35S promoter region of the CaMV ...
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bioRxiv - Genetics 2022Quote: ... Leukocyte genomic DNA (500 ng) was treated with bisulfite using the DNA EZ Methylation-Gold methylation kit (Zymo Research, CA, USA), following the recommendations stipulated by the manufacturer.
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bioRxiv - Bioengineering 2022Quote: cDNA samples were again purified via column purification using a Zymo Research DNA Clean and Concentrator-25 kit (Zymo, Irvine, CA). Select 200-300 bp regions were then amplified using specific primers designed against various hotspot regions found in the patient-sample derived consensus sequence (Supplementary Table 5) ...
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bioRxiv - Evolutionary Biology 2022Quote: RNA was extracted from 30 μL of each virus inoculum using a Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... the DNA fragments were treated twice with bisulfite using an EZ DNA Methylation-Gold™ Kit (Zymo Research, Orange, CA, USA). The libraries were then prepared according to the Illumina standard DNA methylation analysis protocol.
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bioRxiv - Synthetic Biology 2024Quote: RNA was extracted from callus and protoplast cultures using the ZR Plant RNA Miniprep™ kit (R2024, Zymo Research, CA, USA) and TRIzol™ Reagent (15596026 ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from 1.5 mL cultures of Bacteroides strains using the Zymobionics DNA Miniprep kit (Zymo Research, Irvine, CA) and used as input for IS-Seq as described previously 19 ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted from these samples as previously described (19) using a ZymoBiomics DNA Miniprep kit (Zymo Research, Irvine, CA USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Further purification steps were done according to the manufacturer’s guidelines with an on-column DNase I treatment (Zymo Research, Irvine, CA). The RNA was eluted in 40 μL DEPC-treated RNase-free water and stored at -80°C ...
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bioRxiv - Biochemistry 2024Quote: ... purified DNA was subjected to bisulfite modification by means of the EZ DNA Methylation-GoldTM Kit (Zymo Research, Orange, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... 0.15-0.25 grams of stool samples were extracted using the Quick-DNA Fecal/Soil Microbe kit (Zymo Research, Irvine, CA, USA). Negative extraction controls were included to ensure that no exogenous DNA contaminated the samples ...
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bioRxiv - Microbiology 2024Quote: A whole-stranded transcriptome RNA library was generated using the Zymo-Seq RiboFree Total RNA kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... Two micrograms of DNA were subjected to sodium bisulfite conversion using an EZ DNA Methylation Kit (Zymo Research, Irvine, CA, USA). Genomic regions were subsequently amplified using EpiTaq HS (Takara Bio ...
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bioRxiv - Microbiology 2024Quote: DNA was isolated from freshly-grown colonies using the Zymo Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, Irvine, CA, USA). Libraries were prepared using the Nextera DNA Flex Library Prep as recommended by the manufacturer (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... The nested PCR products were then cleaned using a Zymo 96 well Clean and Concentrator Kit (Zymo Corp., Irvine, CA, USA).
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bioRxiv - Pharmacology and Toxicology 2024Quote: Genomic DNA was extracted from the hippocampus using the Quick-DNA/RNA Miniprep Plus kit (Zymo Research, Irvine, CA, United States) and checked for purity and quantity using a NanoDrop spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... DNA extraction was done using a Quick-DNATM fungal/bacterial miniprep kit following the manufacturer’s protocol (Zymo Research, Irvine, CA, USA). The extracted DNA quality was checked by loading 5 µl DNA + 2 µl 6× loading dye with gel red on a 1% agarose gel and separated at 100 V for 40 min ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from TRAP and input samples with TriZol and the Direct-Zol RNA MicroPrep Kit (Zymo Research, Orange, CA).
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bioRxiv - Microbiology 2024Quote: Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16STM NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene ...
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bioRxiv - Genomics 2024Quote: ... one additional library per sample was constructed using the Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research, Irvine, CA, USA). Each library type was pooled and 150 bp paired-end reads generated on an Illumina NextSeq 550 instrument at the University of Saskatchewan Next-Generation Sequencing Facility ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was extracted from 12-day old gametophytes using Quick-DNA Plant/Seed Miniprep Kit (Zymo Research, Irvine, CA, USA). Primers were designed using SnapGene with elongation factor 1 (Ceric.09G081400 ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from Buffy coat fractions and subsequently underwent bisulfite conversion using the EZ DNA Methylation kit (Zymo Research, Irvine, CA). Methylation was then assayed using the Infinium HumanMethylation450 BeadChip (Illumina Inc ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was extracted from each supernatant with the Zymo Quick-DNA/RNA™ Miniprep Plus Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s protocol for tissue samples ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from EV isolation supernatants (i.e. EV-depleted media) was extracted using Zymo ZR urine RNA isolation kit (Zymo Research, Irvine, CA), following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: RNA and DNA were extracted from the same frozen cell pellets using the ZR-Duet DNA/RNA MiniPrep kit (D7001, Zymo, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... those in the correct size range were extracted and purified using a Zymoclean gel DNA recovery kit (Zymo Research, Irvine, CA, USA) and Sanger sequenced (Quintara Biosciences ...
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bioRxiv - Genomics 2020Quote: DNA was extracted using 4-10 unstained slides of FFPE tissue using the Pinpoint Slide DNA Isolation system (Zymo Research, Orange, CA). In brief ...
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bioRxiv - Plant Biology 2020Quote: ... The yeast expression vector constructs or empty vector were transformed into yeast Saccharomyces cerevisiae WAT11 (Pompon et al., 1996; Truan et al., 1993) by using Yeast Transformation II Kit (ZYMO, CA, USA) and screened on synthetic SD-dropout medium lacking uracil (SD-Ura ...
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bioRxiv - Genetics 2021Quote: ... All samples were randomized into 96-well plates for RNA extraction with the Direct-zol-96 RNA Kit (Zymo Research, Irvine, CA). Libraries constructed with the Lexogen QuantSeq 3′ mRNA-Seq Library Kit FWD (Lexogen ...
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bioRxiv - Genetics 2020Quote: 200ng of liver genomic DNA per sample was treated with sodium bisulfite using the EZ DNA Methylation kit (Zymo Research, Irvine, CA). Standards using mouse genomic DNA of known percentage methylation (0 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA was extracted from the pellets and the highly infected samples used the Zymo Quick-RNA kit (Zymo Research LLC, Irvine, CA). Three samples with unfiltered hemolymph ...
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bioRxiv - Plant Biology 2020Quote: ... As a positive control we included a mock microbial community in three wells (ZymoBiomics Microbial Community Standard, Zymo Research, Irvine, CA, USA). We used the Synergy 2.0 Plant DNA Extraction Kit (OPS Diagnostics ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Some DNA extracts that showed high levels of degradation were size-selected using Select-a-Size column or bead kits (ZYMO, Irvine, CA). High molecular weight ...
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bioRxiv - Biochemistry 2020Quote: ... The Carbon-Clarke equation was used to calculate how many colonies were needed to ensure a 99% confidence that all guides in the library was represented after each transformation (∼37,000 colonies).20 Colonies were scraped and midi-prepped (Zyppy Plasmid Midiprep Kit, Zymo Research, Irvine, CA) in preparation to be transformed into strain W.
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bioRxiv - Biochemistry 2021Quote: ... at 10 m/s for 90 s to lyse cells and then using the Zymo Research Direct-Zol RNA MiniPrep Plus kit (Zymo, Irvine, CA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial 16S primers amplified the V3-V4 region of the 16S rRNA gene using primers custom-designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity.
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bioRxiv - Genetics 2021Quote: ... The adaptor-ligated DNA samples were subjected to bisulfite conversion by the EZ DNA Methylation-Gold kit (Zymo Research, Irvine, CA, USA) and amplified by PCR with the KAPA HiFi HotStart Uracil+ ReadyMix kit (Roche ...
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bioRxiv - Microbiology 2021Quote: Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). Bacterial 16S primers amplify the V3-V4 region of the 16S rRNA gene ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 30 min and concentrated to 8μL using the RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA). Full protocol for nucleic acid extraction and subsequent cDNA generation is available at https://www.protocols.io/view/sequence-independent-single-primer-amplification-o-bckxiuxn.
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bioRxiv - Microbiology 2022Quote: ... Fungal DNA from the cultures was extracted using a ZymoBIOMICS® Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2022Quote: ... followed by selective recovery of total RNA using an RNA spin column clean-up kit (RNA Clean & Concentrator-5; Zymo, Irvine, CA). RNA was extracted from cultured cells by phase separation with chloroform followed by RNA precipitation in isopropanol as previously described [38] ...
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bioRxiv - Genomics 2022Quote: ... 500ng of high-quality genomic DNA was bisulfite converted using the Zymo EZ-96 DNA Methylation kit (Zymo Research, Irine, CA, USA). Bisulfite converted samples were then amplified ...
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bioRxiv - Microbiology 2022Quote: Samples were homogenized in an Omni Bead ruptor in TRIzol and then the RNA was extracted from 300μL of each sample using the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA). 2μL of isolated RNA from each sample was then converted to cDNA using the RevertAID first strand cDNA synthesis kit (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 300μl of the resulting coral slurry using ZymoBIOMICS™ DNA/RNA Miniprep (Zymo Research Corp., Irvine, CA, USA) following an additional 2-step enzyme incubation to increase recovery of bacterial DNA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we mixed the flasks by shaking for 1 hr and pelleted the cells for standard plasmid maxiprep (Zymo Research, Irvine, CA, D4201), from which we obtained > 90 μg of purified plasmid.