Labshake search
Citations for Zymo Research :
801 - 850 of 946 citations for Dengue Virus Serotype 1 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... One of each type of swabs was placed into 1 mL DNA/RNA Shield (Zymo Research, Irvine California, USA) and the other of each into 1 mL methanol ...
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bioRxiv - Genomics 2020Quote: RNA and DNA were extracted from the same frozen cell pellets using the ZR-Duet DNA/RNA MiniPrep kit (D7001, Zymo, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Genomic DNA was extracted from cells or tumor tissues and subjected to bisulfite treatment using an EZ DNA Methylation-Gold Kit (ZYMO RESEARCH, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted from SARS-CoV-2 infected and uninfected Huh7 cells using the Direct-zol™ RNA Miniprep (Zymo Research, USA).
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bioRxiv - Biochemistry 2021Quote: ... at 10 m/s for 90 s to lyse cells and then using the Zymo Research Direct-Zol RNA MiniPrep Plus kit (Zymo, Irvine, CA) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... and genomic DNA (gDNA) of surviving cells was isolated using a genomic DNA cleanup kit according to manufacturer instructions (Zymo Research, D4065). For Illumina sequencing and screening analysis ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA from ∼106 parental or sorted CEM/R5 cells or CD4 lymphocytes was purified using Quick-gDNA MiniPrep Kit (Zymo Research, USA). The CXCR4 target regions were PCR-amplified using 200 ng of genomic DNA ...
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bioRxiv - Microbiology 2020Quote: ... RNA from SARS-CoV-2 infected and uninfected Huh7 cells and supernatent was extracted using the Direct-zol RNA Miniprep (Zymo Research, US) and quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Cell Biology 2021Quote: Total RNA from yeast cells grown into SD-Trp-Leu to mid-log phase was isolated with YeaStar RNA Kit (Zymo Research R1002). RNA was treated with 0.02 units/µl DNase I (NEB M0303 ...
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bioRxiv - Cancer Biology 2022Quote: ... and from cell lines using and Direct-zol™ RNA MiniPrep w/ Zymo-Spin™ IIC Columns (Zymo Research, Irvine, CA, USA). ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were extracted from a 10 mL culture (5 OD) of sorted cells subpopulations using the Zymoprep™ Yeast Plasmid Miniprep II (Zymo Research) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... coli cells were recovered by centrifugation and pGFP was purified using ZymoPURE™ Plasmid Maxiprep Kit (catalog #D4203, Zymo Research, Irvine, USA). The DNA concentration was measured in a NanoDrop™ 2000/2000c Spectrophotometer (ThermoScientific® ...
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bioRxiv - Bioengineering 2021Quote: ... control targeted cells (cells that underwent CRISPR/Cas9-mediated targeting and HDR of an expression cassette at a non-GSH locus) and two independent cultures of untargeted cells using Direct-zolTm RNA Miniprep kit (Zymo Research, ZYR.R2052). 1μg of RNA was converted into cDNA with Superscript IV Vilo MM (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA from individual eGFP+ cell lines was extracted from 96 well-plates using Quick-DNA Universal 96 Kit (Zymo Research, D4071) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and 250 μl of this cell suspension was added to 250 μl of lysis buffer in ZR BashingBead™ lysis tubes (Zymo Research) containing 0.1 mm and 0.5 mm beads ...
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bioRxiv - Microbiology 2022Quote: ... gondii infected-PC12 cells that were harvested on day five post-infection using the Direct-zol RNA MiniPrep Plus kit (Zymo Research, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was extracted from HBEC controls and various HBEC derivative cells (three different clones for each group) using a Quick-DNA Miniprep Plus kit (Zymo Research, D4070). DNA samples were submitted to the Van Andel Institute’s Genomics Core (Controls ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: RNA from HEK Flp-In™ T-REx™ 293 and A549 cells was extracted using Zymo III columns (Zymo, Freiburg, Germany). Reverse transcription was performed using random hexamer primers (IDT ...
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bioRxiv - Genetics 2023Quote: ... The amplicons were treated with DpnI restriction enzyme to remove the plasmid template and transformed into XL10 gold competent cells prepared by ‘Mix and Go!’ transformation kit (Zymo Research, T3001). The sequence of the isolated plasmids was confirmed using nanopore sequencing (Plasmidsaurus ...
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bioRxiv - Microbiology 2023Quote: ... we evaluated different commercial DNA extraction kits and procedural modifications by extracting Lehman Caves samples as well as “spike in” experiments in which cells from either an Acidithiobacillus thiooxidans culture or a microbial community standard (ZymoBIOMICS Microbial Community Standard, Zymo Research Corp.) were added to low biomass gypsum collected from the Frasassi Caves.
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from HL-7702 cell lines and 2 μg DNA was treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (ZYMO Research, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from GFP+/DAPI-FAC-sorted transient transfected HEK293T cells with ZR-Duet DNA/RNA MiniPrep Kit (Zymo Research, D7001). RNA-seq libraries were prepared using SMARTer Stranded total RNA-seq Kit v3 (Clontech ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from the flash-frozen cell pellets using the Zymo dual DNA/RNA extraction kit (D7001, Zymo, Irvine, CA, USA). Extractions were performed in batches of 12 where all treatments and timepoints per individual were extracted in the same batch ...
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bioRxiv - Molecular Biology 2023Quote: ... between 400 million to 1.2 billion cells (two to six flash-frozen pellets) were lysed and extracted using the YeaStar Genomic DNA kit (Zymo 11-323), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 40 IVO blastocysts or 80 8-cell embryos were digested and bisulfite converted according to the manufacturer’s instruction of EZ DNA Methylation-Direct Kit (Zymo, Irvine, CA, USA). Bisulfite-specific primers were designed with the online Methyl Primer Express ...
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bioRxiv - Genetics 2023Quote: ... Bead-bound cells were placed on a magnetic stand and DNA-containing supernatant was cleaned using ChIP DNA Clean & Concentrator (Zymo Research, D5201). Transposed libraries were amplified in 50 μl PCRs (25 µl NEBNext HiFi 2× PCR master mix ...
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bioRxiv - Molecular Biology 2024Quote: ... was used to purify genomic DNA from the cells followed by bisulfite conversion using 50 ng DNA along with the EZ DNA Methylation- Gold kit (Zymo Research D5005). 10 ng of bisulfite-converted DNA was used to generate the whole genome bisulfite sequencing (WGBS ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from snap-frozen cell pellets using the Quick-RNA Micro Prep Kit (Zymo Research, Irvine, CA, USA, R1050) and DNase digestion as per manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... RNA and DNA were simultaneously extracted from the cell samples using the Zymo Quick-DNA/RNA Microprep Plus Kit (Zymo Research, D7005) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 1(10)6 lymphocytes were harvested into an equal volume of Trizol and DNA/RNA shield (Zymo Research R110-250). Total RNA was extracted using using Zymo Directzol Microprep (Zymo Research R2060 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Then 1 mL of the culture was removed for DNA extraction using the Zymoprep yeast Plasmid Miniprep II (Zymo Research) kit ...
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bioRxiv - Bioengineering 2020Quote: ... at 37°C for 1 hour to remove the gblock template then purified using a RNA spin column (Zymo Research). Purified RNA was then treated with 200 units of calf intestinal phosphatase (New England Biolabs ...
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bioRxiv - Bioengineering 2021Quote: 1 μg of genomic DNA was hydrolyzed overnight at 37 ºC with 5 U of DNA Degradase Plus (Zymo Research) following the manufacturer’s protocol in 50 μl volume ...
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bioRxiv - Synthetic Biology 2021Quote: For changing cation concentration from 1 to 0 we utilized the ssDNA clean-up kit (ssDNA/RNA clean/concentrator D7010) from Zymo Research such that all the extended ssDNA synthesized in the initial part of the experiment was retained on the column and the TdT ...
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bioRxiv - Synthetic Biology 2021Quote: ... The pQE60::trpCp variants were transformed into the ΔtrpD JW1255-1 strain (BW25113) from the KEIO collection 47 using the Mix & Go! protocol from Zymo Research (Zymo Research ...
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bioRxiv - Systems Biology 2021Quote: ... at which point a 200 µL aliquot was removed and preserved in 1 mL of DNA/RNA Shield (Zymo Research) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Metagenomic libraries were diluted to 1 nM using the Select-a-Size DNA Clean and Concentrator MagBead Kit (Zymo Research) and pooled for further sequencing on the Illumina MiniSeq platform ...
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bioRxiv - Genomics 2022Quote: ... The DNA was digested with 0.5 mg/ml Proteinase K for 1 hour at 65°C and then purified using the ChIP DNA Clean & Concentrator kit (Zymo) and quantified with QUBIT ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted by vortexing for 30 min in elution buffer (1% SDS and 0.1 M NaHCO3) and DNA was purified using a ChIP DNA Clean & Concentrator kit (Zymo Research). For each ChIP ...
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bioRxiv - Evolutionary Biology 2019Quote: 208 female beetles (Table 1) p stored at −80°C were homogenized in Tri-Reagent® (Zymo Research, California, USA) using an electric bead mill ...
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bioRxiv - Microbiology 2022Quote: ... Swabs were vortexed for 10 seconds in 300 μL of 1 × DNA/RNA Shield™ (Zymo Research, Cat. No. R1100) and centrifuged for 5 minutes at 5,000 × g ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR product sizes were verified on a 1% agarose gel prior to PCR clean up (DNA Clean & Concentrator-5, Zymo). A DNA fragment for transcription of lpp (lpp sequence preceded by aforementioned T7 transcription-enabling sequence ...
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bioRxiv - Developmental Biology 2024Quote: ... The DNA was digested with 0.5 mg/ml Proteinase K for 1 hour at 65°C and then purified using the ChIP DNA Clean & Concentrator kit (Zymo) and quantified with QUBIT ...
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bioRxiv - Molecular Biology 2024Quote: ... the aqueous phase was transferred to a fresh tube and 1 volume of 100% ethanol was added and mixed thoroughly before binding to an RNA Clean & Concentrator (Zymo). RNA was DNase I digested on-column (Zymo) ...
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bioRxiv - Genomics 2024Quote: ... tagmentation was performed at 55°C for 5 min after the addition of 1/50uL of N7-adaptor loaded Tn5 and subsequent quenching with DNA binding buffer (Zymo) for 5 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... a small piece of gel (∼100 μl volume) was dissolved in 400 μl TRI-reagent (Zymo Research, R2050-1-200) and the RNA was purified using an RNA isolation kit (Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted and DNAseI-treated from DAC-treated AF2 cells using the ZR-Duet DNA/RNA MiniPrep (Plus) kit (Zymo Research Cat # D7003). This RNA was converted to cDNA using SuperScript III First-Strand Synthesis System (Invitrogen Cat# 18080051 ...
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bioRxiv - Microbiology 2019Quote: ... Total RNA was extracted from the estradiol-grown strain DHT3 cells using the Direct-zol RNA MiniPrep Kit (Zymo Research; Tustin, CA, USA). The crude total RNA was further purified using Turbo DNA-free Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from the sorted cells as described (Hrvatin et al., 2014a) and concentrated using the RNA Clean & Concentrator-5 kit (Zymo Research, Cat# R1013). Complementary DNA (cDNA ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was isolated and extracted from 4IC tumor samples and TU-BcX-4IC adherent cells using Quick-RNA MiniPrepTM (Zymo Research, Irvine, CA) according to manufacture protocol ...