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Citations for Zymo Research :
751 - 800 of 946 citations for Dengue Virus Serotype 1 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... RNA from either CD34+ cells sorted after transduction or bulk cells at day 12 of culture was extracted using Direct-Zol RNA MicroPrep Kit (Zymo Research) and reverse transcription was performed with SuperScript Vilo cDNA Synthesis Kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: Experiments to determine whether CxNV1 has DNA forms were initiated by extracting RNA from CT cells using Direct-Zol RNA MicroPrep (Zymo Research), and separately ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were harvested in TRIzolTM LS reagent (Invitogen) and total RNA was extracted using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... 4sU-labelled mouse and Drosophila cells were mixed and RNA was extracted using the Direct-zol DNA/RNA Miniprep kit (Zymo Research) as per the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using a YeaStar genomic DNA purification kit (Zymo Research). Equal quantities of DNA from four spontaneous canavanine-resistant clones derived from strain 2299 were combined ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from biological triplicates of HMLE-Twist and HMLE-pBp cells was extracted (12 biological samples in total) with Quick-RNA Miniprep kit (Zymo Research) according to manufacturer protocol ...
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bioRxiv - Genetics 2019Quote: ... genomic DNA was isolated from a small aliquot of mixed mouse and fly cells using Quick-DNA Miniprep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2020Quote: ... the cells were washed with PBS three times and RNA was extracted using an RNA extraction kit (Zymo, Irvine, CA, USA). In a parallel experiment ...
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bioRxiv - Microbiology 2020Quote: ... were added to media-removed cell monolayers per single well of 12 well plates followed by on-column DNA digestion and purification (Zymo Research). For RNA purification from RNP capture sample ...
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bioRxiv - Microbiology 2021Quote: ... as well as two replicates of sorted yeast display library based on RBD-binding and expression were extracted from sorted yeast cells using a Zymoprep Yeast Plasmid Miniprep II Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... The remaining cells were lysed with Zymo QuickRNA lysis buffer and isolated with the Zymo QuickRNA micro kit (Zymo, Cat #R1050) using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Total RNA from cultured NSC-34 cells or primary motor neurons was isolated using DirectZole RNA Purification Kit (Zymo Research, R2052), and reverse transcribed using qScript cDNA Synthesis Kit (Quanta Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... human islets or EndoC-βH5 cells using a commercial column-based purification with DNAse I treatment (Direct-Zol RNA MicroPrep with Trizol Reagent, Zymo Research). RNA was quantified by Nanodrop spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: Cells were sorted by FACS as described above and RNA were extracted by using Quick- RNA Microprep Kit (Zymo Research, R1050) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The extracted DNA (100-1000 ng) from anal cells was bisulfite conversion by using EZ kit Gold Bisulfite Conversion Kit (Zymo Research) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: RNA was extracted from cells following CTS or control treatments using the ZR-Duet™ DNA/RNA MiniPrep kit (Zymo D7001). RNA concentration and quality were measured using the Agilent 2100 Bioanalyzer ...
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bioRxiv - Immunology 2021Quote: ... 5µl of each reaction including a negative control without scFv was subsequently transformed into 100 µl Fast Transformation of Mix & Go Competent Cells (Zymo T3001) each and plated on LB plates containing Ampicillin ...
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bioRxiv - Molecular Biology 2020Quote: 15 µl of UltraPure water was added to each assembly reaction on ice and 2.5 µl of these diluted reactions were then transformed into 50 µl chemically competent DH5α cells (prepared in house, Zymo T3001) on ice ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the ligation products were used to transform 20μl of zymo mix&go 10B competent cells (Zymo Research, T3020). Transformed cells were grown in 2ml 2XLB media overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from biological triplicates of HMLE-Twist and HMLE-pBp cells was extracted with Quick-RNA Miniprep kit (Zymo Research) according to manufacturer protocol ...
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bioRxiv - Neuroscience 2022Quote: Viral RNA in supernatants of infected cells and intracellular RNA were extracted using Quick-RNA™ Viral 96 Kit (Zymo Research). RT-qPCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... ATAC-seq libraries from young and old cells were amplified with 11-14 PCR cycles and then purified using the Zymo DNA Clean & Concentrator kit (Zymo, D4014) and eluted in 15 μL of nuclease-free H2O ...
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bioRxiv - Cancer Biology 2023Quote: RNA was extracted from cell lysates using the Quick-RNA MiniPrep kit with on-column DNase I treatment (Zymo Research, USA) according to the mnufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from human iPSC-FBs and mouse Raw 264-7 cells using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Competent cells of these strains were prepared using the Frozen EZ Yeast Transformation II Kit™ (Zymo Research, Irvine, CA, USA) and transformed with a multigene plasmid carrying DlCYP87A4 and ATR2 or human P450scc ...
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bioRxiv - Developmental Biology 2023Quote: Sw.71 cells pellets were lysed and RNA was extracted using the Zymo QuickRNA Microprep kit (Zymo Research, Irvine, CA USA). Whole placentas were equilaterally quartered and homogenized in RNA Lysis buffer and RNA was extracted using the Zymo QuickRNA miniprep kit (Zymo Research ...
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bioRxiv - Immunology 2023Quote: ... pEXG2 suicide vector containing 700-bp sequences of the flanking regions of the selected gene was directly inserted into competent SM10λpir (Mix&Go competent cells, Zymo Research Corporation) and subsequently selected on LB-Agar supplemented with 50 μg/mL kanamycin /15 μg/mL gentamicin ...
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bioRxiv - Genomics 2022Quote: ... At the indicated time points we harvested cells and extracted RNA using the Direct-zol-96 RNA kit (Zymo #11-331H). Samples were then sent to Novogene for bulk RNA-seq.
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from 2-5 x107 cells grown in different nutrients (see above) with the YeaStar RNA Kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 350 ng of each extracted sperm DNA and 350 ng of control human NA12878 lymphoblastoid cell line genomic DNA (Coriell Institute) were bisulfite-converted using the Zymo EZ DNA Methylation kit (Zymo Research). The loci were amplified by PCR using the following primer sets ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids used for transient transfections in HEK293T cells were prepared and purified using the Zymopure Midiprep Kit (Zymo Research, cat #D4201). Mutations to create the CFTR ΔPRF constructs were chosen using RNA structure predictions performed by the ViennaRNA RNAfold Web Server.
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bioRxiv - Biochemistry 2023Quote: A standard whole-cell mixture consisting of 21 representative strains from 17 species of the gut microbiota (Zymo Research, ref D6331) was divided into 10 aliquots and stored in the storage solution provided by the manufacturer at −80 °C ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from cultured cells or myocardial tissue of mice euthanized at the timepoints reported above using the Direct-zol RNA purification Kit (ZYMO Research) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... were grown at 28°C to stationary phase and cell pellets from 5 mL cultures were collected for total RNA extraction using a Direct-zol RNA miniprep kit (Zymo Research). 10 μg of isolated total RNA was subjected to DNaseI treatment with 20 units of Turbo DNaseI (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... S4) and cells were collected by filtration for RNA purification using the RNA Clean and Concentrator kit (Zymo Research, Irvine CA). rRNA depletion was accomplished with the Ribo-Zero rRNA Removal Kit (Epicentre ...
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bioRxiv - Microbiology 2024Quote: The infected cells or homogenized lungs were lysed in Trizol to prepare total RNAs using Direct-Zol RNA Microprep kit (Zymo Research) with DNase treatment according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The bacmid encoding Halo-Ku80/Ku70 complex was obtained by electroporating pAM086 into DH10EMBacY electro-competent cells and purified using ZR BAC DNA miniprep kit (Zymo Research).
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bioRxiv - Genomics 2024Quote: ... secondary plant compounds hampering the activity of the pores in the Oxford Nanopore Technologies (ONT) flow cells were removed with the Quick-DNA HMW MagBead Kit (Zymo Research). Sequencing libraries were prepared with the LSK-109 ligation library preparation kit and sequencing was performed on MinION R.9.4.1 flow cells ...
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bioRxiv - Immunology 2024Quote: ... Genomic DNA was isolated from previously frozen cell pellets and bisulfite converted using the EZ DNA methylation-direct kit (Zymo Research). Two PCRs were performed with the PyroMark PCR kit (Qiagen ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were run on 1% agarose gel and purified using the Zymoclean gel DNA recovery kit (Zymo Research).
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bioRxiv - Microbiology 2022Quote: ... RNA extractions were performed from 1 ml LB culture using the Direct-zol RNA MiniPrep Plus (Zymo Research, ZR2073). Total RNAs were reverse-transcribed into cDNAs using iScript™ cDNA Synthesis Kit (BioRad ...
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bioRxiv - Systems Biology 2022Quote: ... The flow through was mixed with 1 volume of ethanol then transferred to a Zymo Spin IIC column (Zymo) and centrifuged ...
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bioRxiv - Biochemistry 2020Quote: ... The entire PCR reaction is run on 1% agarose gel and the DNA at 230bp was purified (Zymo Research). See Supplemental Figure S2.
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bioRxiv - Immunology 2021Quote: ... Cxcl1 promoter regions #1 and #2 were PCR amplified from sodium bisulfite converted gDNA using ZymoTaq Polymerase (Zymo Research) and purified by gel extraction using the QIAquick Gel Extraction Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... cerevisiae eby100 was retransformed using 1 µg of pGal1 – Aga2p_eGFP following the protocol of the EZ yeast transformation kit II (Zymo). 100 µL of cells were selected on SDCAA plates ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant and pellet samples on non-denaturing 1% agarose gel we extracted RNA with TRI reagent® (ZYMO Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant and pellet samples on non-denaturing 1% agarose gel we extracted RNA with TRI reagent® (ZYMO Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteinase K is diluted 1:19 in water and added directly to the column matrix (Zymo-I = 20μL, Zymo-II = 50μL ...
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bioRxiv - Plant Biology 2023Quote: RNA was purified from seedlings Col-0 and cpk32-1 using the Direct-zol RNA MiniPrep Kit (Zymo Research). The reverse-transcriptase cDNA synthesis reaction was carried out by the RevertAid Reverse Transcriptase (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: Bacterial RNA was extracted from ∼1 × 108 CFU after AMP exposure (above) using Direct-zol RNA Miniprep kit (Zymo) as per manufacturer’s instructions ...