Labshake search
Citations for Zymo Research :
6601 - 6650 of 6691 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Swabs were placed in cryotubes containing 1 mL of DNA/RNA Shield (Zymo Research, Irvine, CA, USA) for inactivation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: RNA was isolated from the cell culture supernatant of SARS-CoV-2-infected cells using the Quick-RNA Viral Kit (Zymo, California, USA cat no. R1035) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: 200 ng of mouse E14 genomic DNA sheared to 15 kb fragments were treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA, USA Research), or an EpiTect Bisulfite Kit (QIAGEN ...
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bioRxiv - Biochemistry 2020Quote: ... Genomic DNA of bacterial samples as well as eukaryote cells was purified using the Quick-DNA™ Miniprep Plus Kit (Zymo Research, Irvine, California, US) using the recommendations of the manufacturer.
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bioRxiv - Microbiology 2019Quote: ... The Ribo-Zero rRNA Removal Kit (Bacteria) (illumina®, San Diego, CA) was used for mRNA enrichment and the RNA Clean & Concentrator™ −5 (Zymo Research, Irvine, CA) for final purification ...
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bioRxiv - Microbiology 2020Quote: ... The final pool volume was reduced to concentrate the sequencing library using the DNA Clean and Concentrator™-5 kit (Zymo Research, Irvine, CA, USA). The pooled library concentration was determined using the Quant-iT™ High-Sensitivity DNA Assay Kit (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: The PCR products were checked on an agarose gel stained with ethidium bromide before gel extraction (Zymoclean™ Gel DNA Recovery Kit, Zymo Research, Irvine, CA, USA). The resulting pure amplicons were ligated into pCR™-Blunt II-TOPO® (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was then transferred to a new tube and further processed on columns using the Direct-zol™ Kit (Zymo Research, Irvine, CA, USA). RNA was treated in-column with DNAse and eluted in nuclease-free water before being stored at −80°C ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from the filtered membranes and from 150–200mg of the nonhuman fecal samples using the ZR Fecal DNA MiniPrep™ Kit (Zymo Research, Irvine, CA, USA), as previously described (Kongprajug et al. ...
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bioRxiv - Molecular Biology 2024Quote: A total of 1μg of extracted genomic DNA was bisulfite-converted with the EZ DNA Methylation Kit (D5001) and modified according to the manufacturer’s recommendations (Zymo Research, CA USA & Illumina, CA USA). These samples were run on the Infinium Mouse Methylation BeadChip Array following the Illumina Infinium HD Methylation protocol ...
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bioRxiv - Genomics 2022Quote: ... total RNA was extracted from peripheral white blood cells using the Zymo Research Direct-zol™ DNA/RNA Miniprep kit (Zymo Research Corporation, Irvine, CA), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The barcoded PCR products were further pooled and gel-extracted using Zymo gel extraction kit and DNA clean & concentrator (Zymo research 11-301, 11-303) and quantified by Qubit 1× dsDNA HS assay kits (ThermoFisher Q32851) ...
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bioRxiv - Microbiology 2023Quote: ... A total of 500 ng of genomic DNA from cells in co-cultures were bisulfite-treated using the ZymoResearch EZ DNA Methylation kit (Zymo Research Corp, Irvine, CA, USA). The Infinium HD Methylation Assay (bisulfite modification ...
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bioRxiv - Plant Biology 2023Quote: The bisulfite conversion of sonicated genomic DNA fragments was carried out based on the instructions provided in the EZ DNA-methylation lightning Kit (Zymo Research Corp., Irvine, CA, USA). The libraries were made using the Accel-NGS Methyl-Seq DNA library kit (SWIFT Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... The parasites were washed twice with PBS prior to RNA extraction and purification using the Quick-RNATM MiniPrep kit (Zymo Research, Irvine, CA, United States) according to manufacturer specifications ...
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bioRxiv - Microbiology 2023Quote: DNA extraction was performed from 4g of frozen bedding samples (-80°) or from teat skin swabs with the Quick-DNA™ Fecal/Soil Microbe 96 kit (Zymo Research, Irvine, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... An aliquot of each lung homogenate (300µL) was added to 100µL of TRIReagent (Zymo Research R2050-1-200) and stored at −80°C ...
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bioRxiv - Immunology 2022Quote: ... 500μL of nasal wash was added to 500μL of TRIzol reagent (Zymo Research Catalog No R2050-1-200) and the remainder of the nasal wash was stored for serological analysis ...
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Macrobdella decora: Old World Leech Gut Microbial Community Structure Conserved in a New World LeechbioRxiv - Microbiology 2019Quote: ... 1) Amplification of a dilution series of a ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Irvine, CA U.S.A.), 2 ...
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bioRxiv - Microbiology 2024Quote: ... the PCR master mix has been treated with 0.02% (v/v) of DNAse I 1 U/mL (Zymo) at room temperature for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 70∼80% cells were positive for Thy1.1 after sorting and lysed with TRI reagent (Zymo Research #R2050-1) for RT-qPCR.
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bioRxiv - Plant Biology 2020Quote: ... RNA was isolated using the TRIzol® Reagent and RNA was purified through spin columns of Direct-zol™ RNA MicroPrep kit (Zymo Research, Irvine, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Viral pellets were then resuspended in 200 μl PBS and viral RNA was extracted using Quick-RNA Viral Kit (Zymo Research, CA. Cat. No. R1034 & R1035) following the manufacturer’s protocol and eluted in 10 μl RNase-free water ...
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bioRxiv - Zoology 2021Quote: ... The supernatant was collected and added to 700 μL of 95% ethanol for total RNA recovery using the Direct-zol™ RNA mini-prep kit (Zymo Research®, Irvine, CA, USA). The solution was transferred to filter columns and centrifuged (16,000 g × 30 s) ...
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bioRxiv - Microbiology 2022Quote: ... a negative control to account for microbial contaminants present in DNA extraction kits or introduced during the extraction (Eisenhofer et al., 2019) and a microbial community standard (Zymo Research, cat. no. D6300, Irving, CA). The DNA concentration was quantified with Qubit 3 using the high sensitivity dsDNA kit (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and libraries were pooled and purified twice after gel extraction with the Zymo gel extraction kit and DNA Clean and Concentrator (Zymo Research #11-301 and #11-303). Pooled amplicons were then sequenced on an Illumina MiniSeq system (300 cycles ...
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bioRxiv - Microbiology 2023Quote: ... we generated a mock microbiota community suspension using two commercially available reference standards: the first was the ZymoBIOMICS™ faecal reference kit with TruMatrix™ technology (D6323; Zymo Research, Irvine CA, USA); DNA content ...
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bioRxiv - Molecular Biology 2022Quote: ... The barcoding PCR reactions were further pooled and gel-extracted using Zymo gel extraction kit and DNA clean & concentrator (Zymo research Cat. #: 11-301 and 11-303) and quantified by Qubit 1x dsDNA HS assay kits (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... RNA extractions were performed from 1 ml LB culture using the Direct-zol RNA MiniPrep Plus (Zymo Research, ZR2073). Total RNAs were reverse-transcribed into cDNAs using iScript™ cDNA Synthesis Kit (BioRad ...
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bioRxiv - Systems Biology 2022Quote: ... The flow through was mixed with 1 volume of ethanol then transferred to a Zymo Spin IIC column (Zymo) and centrifuged ...
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bioRxiv - Biochemistry 2020Quote: ... The entire PCR reaction is run on 1% agarose gel and the DNA at 230bp was purified (Zymo Research). See Supplemental Figure S2.
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bioRxiv - Immunology 2021Quote: ... Cxcl1 promoter regions #1 and #2 were PCR amplified from sodium bisulfite converted gDNA using ZymoTaq Polymerase (Zymo Research) and purified by gel extraction using the QIAquick Gel Extraction Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant and pellet samples on non-denaturing 1% agarose gel we extracted RNA with TRI reagent® (ZYMO Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant and pellet samples on non-denaturing 1% agarose gel we extracted RNA with TRI reagent® (ZYMO Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteinase K is diluted 1:19 in water and added directly to the column matrix (Zymo-I = 20μL, Zymo-II = 50μL ...
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bioRxiv - Genomics 2023Quote: ... One of each type of swabs was placed into 1 mL DNA/RNA Shield (Zymo Research, Irvine California, USA) and the other of each into 1 mL methanol ...
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bioRxiv - Neuroscience 2024Quote: ... brains were transferred to tubes containing digestion buffer (0.05% Collagenase D Sigma-Aldrich Cat. C0130-500MG; 0.1µM TLCK, Sigma-Aldrich Cat. T7254-100MG, 10µg/mL DNAse 1, Zymo Cat ...
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bioRxiv - Microbiology 2022Quote: ... 1(10)6 lymphocytes were harvested into an equal volume of Trizol and DNA/RNA shield (Zymo Research R110-250). Total RNA was extracted using using Zymo Directzol Microprep (Zymo Research R2060 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Then 1 mL of the culture was removed for DNA extraction using the Zymoprep yeast Plasmid Miniprep II (Zymo Research) kit ...
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bioRxiv - Bioengineering 2020Quote: ... at 37°C for 1 hour to remove the gblock template then purified using a RNA spin column (Zymo Research). Purified RNA was then treated with 200 units of calf intestinal phosphatase (New England Biolabs ...
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bioRxiv - Bioengineering 2021Quote: 1 μg of genomic DNA was hydrolyzed overnight at 37 ºC with 5 U of DNA Degradase Plus (Zymo Research) following the manufacturer’s protocol in 50 μl volume ...
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bioRxiv - Synthetic Biology 2021Quote: ... The pQE60::trpCp variants were transformed into the ΔtrpD JW1255-1 strain (BW25113) from the KEIO collection 47 using the Mix & Go! protocol from Zymo Research (Zymo Research ...
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bioRxiv - Systems Biology 2021Quote: ... at which point a 200 µL aliquot was removed and preserved in 1 mL of DNA/RNA Shield (Zymo Research) following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2020Quote: ... except the first step started from yeast plasmid prepared from 5×107 to 1×108 cells by Zymoprep (Zymo Research). Illumina adapters and 6-bp pool-specific barcodes were added in the second qPCR step ...
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bioRxiv - Biophysics 2021Quote: ... Sufficient amounts of DNA were obtained growing 1 L of transfected XL1 Blue Escherichia coli cells overnight and performing a gigaprep (Zymo). For protein expression ...
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bioRxiv - Evolutionary Biology 2019Quote: 208 female beetles (Table 1) p stored at −80°C were homogenized in Tri-Reagent® (Zymo Research, California, USA) using an electric bead mill ...
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bioRxiv - Genetics 2020Quote: ... total RNA from approximately 1 × 108 cells was extracted using the Quick-RNA Fungal/Bacterial Miniprep (Zymo Research, Irvine, CA). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... Swabs were vortexed for 10 seconds in 300 μL of 1 × DNA/RNA Shield™ (Zymo Research, Cat. No. R1100) and centrifuged for 5 minutes at 5,000 × g ...
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bioRxiv - Molecular Biology 2024Quote: ... the aqueous phase was transferred to a fresh tube and 1 volume of 100% ethanol was added and mixed thoroughly before binding to an RNA Clean & Concentrator (Zymo). RNA was DNase I digested on-column (Zymo) ...
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bioRxiv - Genomics 2024Quote: ... tagmentation was performed at 55°C for 5 min after the addition of 1/50uL of N7-adaptor loaded Tn5 and subsequent quenching with DNA binding buffer (Zymo) for 5 min at room temperature ...