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Citations for Expression Systems :
301 - 350 of 358 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... First passage (P1) recombinant baculovirus was generated by transfecting Sf9 cells (Expression Systems) with bacmid DNA complexed using linear polyethylenimine (PEI MAX ...
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bioRxiv - Cell Biology 2021Quote: ... Sf9 cells were maintained in suspension culture with ESF-921 medium (Expression Systems) in a shaking incubator at 27°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... using the BestBac baculovirus system (Expression Systems) according to manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The Tni insect cells were grown to a density of 4 million cells/ml in ESF 921 serum free media (Expression systems) before infection with a 4:2:1 ratio of CCK1R:DNGαS:Gβ1γ2 baculoviruses ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Gβ1γ2 were co-expressed in the Tni insect cell line (Expression systems) using the Bac-to-bac baculovirus system (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... BestBac 2.0 Δ v-cath/chiA Linearized Baculovirus DNA and Expres2 TR Transfection Reagent (Expression Systems) to produce the final recombinant baculovirus.
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bioRxiv - Biochemistry 2021Quote: ... and High Five cells were maintained in ESF 921 Insect Cell Culture Medium (Expression System). Human embryonic kidney 293T cells (HEK-293T ...
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bioRxiv - Biophysics 2020Quote: ... 1μg of recombinant bacmid DNA was combined in 1 mL of ESF-921 media (Expression Systems, Cat. #96-001-01) with 20 μL of Cellfectin II (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... Established BMEC lines were transduced with GFP-tagged RNAi lentivirus (BLOCK-iT HiPerform Lentiviral Pol II miR RNAi Expression system, Invitrogen Cat# K4934-00) targeting Raptor or Rictor ...
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bioRxiv - Cell Biology 2020Quote: ... Blue colonies were screened for the presence of inserts by colony PCR using pUC/M13 Forward and Reverse oligos (Bac-to-Bac® Baculoviruses Expression System_invitrogen user guide). To generate baculoviruses ...
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bioRxiv - Biochemistry 2020Quote: ... Sf21) or from Expression Systems (Hi5) and used as identified by the vendor ...
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bioRxiv - Molecular Biology 2020Quote: ... Spodoptera frugiperda (Sf9) were grown in suspension in ESF921 medium (Expression Systems, California), infected at a density of 2-3 × 106/mL with a single baculovirus encoding all three subunits (Gαi1β1γ1) ...
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bioRxiv - Molecular Biology 2020Quote: ... All plasmids were co-transfected with linearized 2.0 DNA (deficient in v-cath/chiAgenes) (Expression Systems, CA, USA) into Sf9 insect cells (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... according to the protocol provided by the manufacturer (Expression Systems). The transfection supernatant was harvested and amplified 2 times to obtain a high titer of the recombinant viruses ...
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bioRxiv - Molecular Biology 2020Quote: ... were cultured in ESF921 medium (Expression Systems) and infected with recombinant virus at an multiplicity of infection (MOI ...
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bioRxiv - Molecular Biology 2020Quote: ... were subcloned into pAC8-derived vectors34 and baculovirus was generated in Spodoptera frugiperda Sf9 cells grown in ESF921 medium (Expression systems) following the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2020Quote: ... either control or Per2 shRNA as well as viral packaging vectors were transfected into HEK293/T17 cells according to the manufacturer’s instruction (ViraPower Lentiviral Expression System, Life Tech). Cell culture media was collected after 48 hours ...
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bioRxiv - Biochemistry 2020Quote: Sf9 insect cells (Expression Systems) were grown in suspension in ESF 921 media (Expression Systems ...
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bioRxiv - Biochemistry 2020Quote: ... were grown in suspension in ESF 921 media (Expression Systems) to a density of 1×106 cells per ml and then infected with baculovirus encoding the same full-length mouse CST construct as described above at a ratio of 40 μL virus per ml of media (this ratio was empirically determined from titer trials in order to optimize protein expression) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell cultures were grown in ESF 921 serum-free medium (Expression Systems) to a density of 2-3 million cells per ml and then infected with three separate baculoviruses at a ratio of 1:2:2 for nsp12 ...
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bioRxiv - Biochemistry 2020Quote: ... Baculovirus was generated using Spodoptera frugiperda Sf9 insect cells (unauthenticated and untested for mycoplasma contamination, Expression Systems 94-001F) and the construct was expressed in Spodoptera frugiperda Sf9 insect cells ...
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bioRxiv - Cell Biology 2020Quote: ... Sf9 insect cells were grown in ESF921 media (Expression Systems) plus antibiotic-antimycotic at 28°C ...
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bioRxiv - Biochemistry 2020Quote: ... A recombinant baculovirus for Gαs subunit was prepared using the BestBac™ (Expression Systems) strategy ...
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bioRxiv - Biochemistry 2020Quote: ... cell suspension cultures adapted to grow in a serum-free medium (ESF, Expression Systems). Transfections were performed using linear polyethylenimine (PEI MAX ...
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bioRxiv - Biochemistry 2020Quote: All NT and Agrin constructs were produced via transient transfection of SFM-HEK293 (Expression Systems) cell suspension cultures adapted to grow in a serum-free medium (ESF ...
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bioRxiv - Biochemistry 2020Quote: ... Sf9 and High Five cells were cultured in ESF 921 medium (Expression Systems, 96-001-01) in a shaker at 140 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... which was amplified once to give a V1 virus stock used to infect 1 l cultures of Sf9 cells (Expression Systems). Cells were grown in ESF 921 Insect Cell Culture Medium Protein Free (expression systems 96-001-01 ...
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bioRxiv - Bioengineering 2020Quote: The RBD (residues 319-541) of the SARS-Cov-2 S protein was expressed as a secreted protein in Spodoptera frugiperda Sf9 cells (Expression Systems) using the Bac-to-bac baculovirus method (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: Hi5 cells (600 mL) were grown in ESF-921 media (Expression Systems) and infected with V1 virus for full-length Chd1 (tagged with a N-terminal 6×His tag ...
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bioRxiv - Biochemistry 2020Quote: ... Expression was performed by transducing recombinant baculoviruses into Sf21 insect cells (Expression Systems). Cells were harvested by centrifugation and resuspended in 25 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... an Avi tag and XbaI and used to generate Baculovirus using the BestBac method (Expression Systems). Spodoptera frugiperda (Sf9 ...
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bioRxiv - Microbiology 2020Quote: ... and cloned into pVL1393 (Expression Systems, CA, USA) with an insect cell secretion signal ...
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bioRxiv - Microbiology 2020Quote: ... as described in the protocol of the BestBactm 2.0 Δ v-cath/ChiA Baculovirus Cotransfection Kit (Expression Systems, CA, USA). P1 baculovirus was generated with suspended Sf9 cells (2 ×106 cells/ml ...
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bioRxiv - Microbiology 2020Quote: ... Viral titer was measured using a flow cytometric assay for gp64 expression (Expression Systems, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... A recombinant baculovirus (P0) was produced in SF9 cells (Expression Systems, CA, USA) as described in the protocol of the BestBactm 2.0 Δ v-cath/ChiA Baculovirus Cotransfection Kit (Expression Systems ...
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bioRxiv - Biophysics 2020Quote: ... or alternatively in ESF media (Expression Systems) at 27°C ...
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bioRxiv - Molecular Biology 2020Quote: ... were seeded per well in a 6-well plate in protein-free ESF 921 media (Expression Systems Cat# 96-001) with no additives or antibiotics ...
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bioRxiv - Molecular Biology 2020Quote: ... 2×106 Sf9 cells (Expression Systems Cat# 94-001S) were seeded per well in a 6-well plate in protein-free ESF 921 media (Expression Systems Cat# 96-001 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and then used to infect 1 L of Sf21 cells (Expression Systems) and incubated for 48 hrs at 27°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The bacmid was transfected into Sf9 cells (Expression Systems) with Cellfectin II (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: Spodoptera frugiperda (Sf9) insect cells (Expression Systems) were co-infected at a density of ~2.0 × 106 cells/mL with HA-FLAG-GABAB2 baculovirus and either HA-FLAG-GABAB1b-His6 or HA-GABAB1b-His6 baculovirus at a multiplicity of infection (M.O.I. ...
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bioRxiv - Microbiology 2020Quote: ... ni cells grown in ESF-921 media (Expression Systems). Conditioned media was collected 60 h post-infection ...
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bioRxiv - Biophysics 2020Quote: ... were grown in ESF921 medium (Expression Systems) at 28 °C without CO2 exchange and in absence of antibiotics ...
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bioRxiv - Biophysics 2020Quote: ... Sf9 cells (Expression System) were grown in ESF921 medium (Expression Systems ...
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bioRxiv - Cell Biology 2020Quote: ... and in adherent culture on T-25 tissue culture flasks in Grace’s media (Gemini Bio-Products; Expression systems) with 10% FBS (Gemini Bio-Products ...
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bioRxiv - Cell Biology 2020Quote: Spodoptera frugiperda ovarian-derived Sf9 cells were maintained in suspension culture in ESF 921 media (Expression Systems, Davis, CA) and in adherent culture in Grace’s media (Gemini Bio-Products ...
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bioRxiv - Immunology 2020Quote: ... Plasmids for each chain were separately transfected into SF9 insect cells with BestBac 2.0 baculovirus DNA (Expression Systems; Davis, CA) and Cellfectin II reagent (Thermo Fisher ...
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bioRxiv - Biochemistry 2020Quote: Spodoptera frugiperda (Sf9) insect cells were maintained in ESF 921 media (Expression Systems) in vented Erlenmeyer flasks (Corning ...
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bioRxiv - Biochemistry 2020Quote: ... and 100 µL of transfection medium (Expression Systems), and incubated for 96 h with 300 rpm shaking at 27°C ...
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bioRxiv - Biochemistry 2020Quote: ... The percentage of cells infected by virus was quantified by flow cytometry following staining of 10 µL of cells with 10 µL of PE-conjugated anti-gp64 antibody (Expression Systems, catalog #97-201) for 20 min in the dark at 4°C ...