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Citations for Expression Systems :
251 - 300 of 358 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: Spodoptera frugiperda (Sf9) insect cells (Expression Systems) were cultured in ESF 921 serum-free medium (Expression Systems ...
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bioRxiv - Biochemistry 2021Quote: ... Sf9 cells were cultured at 27 °C in 1 L culture flasks with ESF-921 insect cell culture medium (Expression systems). Next ...
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bioRxiv - Cell Biology 2021Quote: His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...
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bioRxiv - Biochemistry 2021Quote: ... BestBac 2.0 Δ v-cath/chiA Linearized Baculovirus DNA and Expres2 TR Transfection Reagent (Expression Systems) to produce recombinant baculovirus ...
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bioRxiv - Biochemistry 2021Quote: ... Protein expression was induced by baculovirus infection of 6-8 liter cultures of Sf9 cells in ESF921 medium (Expression Systems) at a density of ~2 × 106 cells/ml ...
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bioRxiv - Biophysics 2021Quote: Baculovirus expressing SSTR2KappaICL3-GFP at P2 was used to infect Sf9 insect cells (Expression Systems) at a density of 3–4 million cells/ml and incubated at 28C with shaking ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were produced using in vitro transcription and translation system using wheat germ extract following manufactures’ instructions (SP6 High Yield Expression System from Promega). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... Both wild-type and kinase-dead (D286A) Sgk3 were expressed in baculvirus-infected Sf9 insect cells grown in ESF 921 medium (Expression Systems) at 27°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... This receptor construct was expressed in Sf9 insect cells (Expression Systems) using the BestBac baculovirus system (Expression Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... The wild-type BTK construct was cloned in the base vector (Lenti-X Expression System Version EF1-a; Takara Bio). STable over-expression of BTK in ibrutinib-resistant DLBCL cells was performed by using lentiviruses expressing human wild-type BTK as described previously.[15]
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bioRxiv - Biophysics 2021Quote: ... and the virus was prepared using the BestBac system (Expression System, LLC). N-terminal Flag-tagged human Gβ1 ...
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bioRxiv - Biochemistry 2021Quote: ... and sNrk was expressed in Sf9 cells grown in ESF921 medium (Expression Systems). Conditioned medium was collected 3 days after infection ...
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bioRxiv - Biochemistry 2021Quote: Recombinant baculoviruses encoding Drl family ECRs were used to infect Sf9 cells (T. ni cells for sDrl-2 for crystallization) grown in ESF921 medium (Expression Systems). Conditioned medium was harvested three days after infection and subjected to extensive dialysis at 4°C against 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Cell cultures were grown in ESF 921 serum-free medium (Expression Systems) to a density of 2-3 million cells per ml and then infected with four separate baculoviruses at a suitable ratio ...
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bioRxiv - Biophysics 2021Quote: ... and scFv16 were co-expressed in Trichoplusia ni Hi5 insect cells using the baculovirus method (Expression Systems). Cell cultures were grown in ESF 921 serum-free medium (Expression Systems ...
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bioRxiv - Biophysics 2021Quote: ... Cell cultures were grown in ESF 921 serum-free medium (Expression Systems) to a density of 2-3 million cells per ml and then infected with four separate baculoviruses at a suitable ratio ...
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bioRxiv - Biophysics 2021Quote: ... and Gγ2 were co-expressed in Trichoplusia ni Hi5 insect cells using the baculovirus method (Expression Systems). Cell cultures were grown in ESF 921 serum-free medium (Expression Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... insect cells were grown in ESF 921 culture medium (Expression Systems) to a density of 3.2 × 106 cells/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... were cultured in ESF 921 serum-free medium (Expression Systems) at 27 C and 120 rpm.
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bioRxiv - Plant Biology 2021Quote: ... Plasmid-DNA was isolated with the ZymoPURE Plasmid Midiprep (ZymoGenetics, Seattle, Washington, United States The HY5 protein was expressed with TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... C2 and C3 in Hi5 cells (Expression systems). After 60 hours of expression ...
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bioRxiv - Molecular Biology 2021Quote: ... insect cells were grown in ESF 921 culture medium (Expression Systems) to a density of 3.2 × 106 cells/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... GIPR and Gs heterotrimer were co-expressed in High Five™ cells grown in ESF 921 culture medium (Expression Systems) to a density of 3.2 × 106 cells/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... and High Five™ insect cells (Expression Systems) were cultured in ESF 921 serum-free medium (Expression Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... were cultured in ESF 921 serum-free medium (Expression Systems) at 27°C and 120 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... His6-tagged human Gβ1and Gγ2 were expressed in Tni insect cells (Expression systems) using baculovirus as previously described ...
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bioRxiv - Biochemistry 2021Quote: ... Cell cultures were grown in ESF 921 serum-free media (Expression Systems) to a density of 4 million cells/ml and then infected with three separate baculoviruses at a ratio of 2:2:1 for GLP-1R ...
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bioRxiv - Biophysics 2021Quote: ... Cell cultures were grown in ESF 921 serum-free medium (Expression Systems) to a density of 3~4 × 106 cells/ml ...
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bioRxiv - Biochemistry 2021Quote: ... Infected cells were typically grown for 48 hours at 27°C in ESF 921 Serum-Free Insect Cell Culture medium (Expression Systems, Cat# 96-001-01). Cells are harvested by centrifugation ...
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bioRxiv - Biochemistry 2021Quote: ... insect cells plated for 24 hours in a Corning 6-well plastic dish (Cat# 07-200-80) containing 2 mL of ESF 921 Serum-Free Insect Cell Culture media (Expression Systems, Cat# 96-001, Davis, CA.). All media contains 1x concentration of Antibiotic-Antimycotic (Gibco/Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... were cultured in Express Five™ SFM (Serum-Free Media) medium (Gibco #B85502 Expression Systems) at a cell density of 0.5 x 106 cells/mL and infected with recombinant virus ...
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bioRxiv - Biochemistry 2021Quote: Spodoptera frugiperda Sf9 cells were maintained as suspension cultures at 27 °C in ESF-921™ Insect Cell Culture Medium (Expression Systems). Isolation of bacmid DNA ...
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bioRxiv - Biophysics 2021Quote: ... Sf9 cells were cultured in ESF 921 medium (Expression Systems, Davis, CA) and P1 virus was generated from cells transfected with Escort IV reagent (MillaporeSigma ...
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bioRxiv - Biochemistry 2021Quote: ... The virus titers were measured by flow cytometry assay (Expression Systems).
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bioRxiv - Biochemistry 2021Quote: One or two liters of Trichoplusia (Tni) cells (Expression System) were infected with recombinant baculoviruses at a cell density of 1.5-2.0 × 106 cells per mL ...
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bioRxiv - Biochemistry 2021Quote: The Gβ1γ2 heterodimer was expressed in Tnchoplusia ni (Hi5) insect cells (Expression Systems, unauthenticated and untested for mycoplasma contamination ...
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bioRxiv - Biochemistry 2021Quote: ... in Spodoptera frugiperda (Sf9) insect cells (Expression Systems 94-001F ...
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bioRxiv - Biochemistry 2021Quote: ... A single bicistronic baculovirus encoding the human Gβ1 subunit with a N-terminal 6x His-tag and rhinovirus 3C protease site and untagged human Gγ2 subunit was generated using the BestBac method (Expression systems) in Spodoptera frugiperda (Sf9 ...
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bioRxiv - Molecular Biology 2021Quote: ... BriSΔ was produced with the MultiBac baculovirus expression system (Geneva Biotech)43 in Hi5 cells using ESF921 media (Expression Systems Inc.). Supernatants from transfected cells were harvested 3 days post-transfection by centrifugation of the culture at 1000g for 10 min followed by another centrifugation of supernatant at 5000g for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... were grown in ESF 921 serum-free medium (Expression Systems) at 27°C and 120 rpm ...
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bioRxiv - Biophysics 2021Quote: Spodoptera frugiperda 9 (Sf9) insect cells (Expression Systems) were grown in ESF 921 serum-free medium (Expression Systems ...
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bioRxiv - Biophysics 2021Quote: ... sf9 cells (Expression System) was infected with baculovirus at a density of 4×106 cells/mL and incubated for 60 hours at 27 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Sf9 cells were cultured in ESF 921 medium (Expression Systems) at 27 °C.
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bioRxiv - Cell Biology 2021Quote: ... Sf9 insect cells (Expression Systems, Davis, CA, US, 94-001F) were grown to a density of 4x106 cells/mL in ESP 921 media (Expression Systems ...
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bioRxiv - Cell Biology 2021Quote: ... were grown to a density of 4x106 cells/mL in ESP 921 media (Expression Systems, 96- 001-01) at 27 °C ...
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bioRxiv - Molecular Biology 2021Quote: Expression of recombinant RDR2 was optimized using the TEQC method (62) in which a 200 ml culture of Hi5 cells (Expression Systems, Inc) was infected at an estimated multiplicity of infection (eMOI ...
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bioRxiv - Molecular Biology 2021Quote: Spodoptera frugiperda (Sf9) insect cells (Expression Systems) were grown in ESF 921 serum-free medium (Expression Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... were grown in ESF 921 serum-free medium (Expression Systems) at 27°C and 120 rpm ...
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bioRxiv - Molecular Biology 2021Quote: HMCES-FLAG proteins were expressed in 250 mL suspension cultures of Sf9 cells (Expression Systems) by infection with baculovirus expressing rHMCES-FLAG for 72 hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... were transiently transfected with ExpiFectamine into 2.5×107 Expi293F cells in 10ml medium according to manufacturer’s protocol (Gibco Expi293 Expression System, Thermo Fisher). One day after transfection ...