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Citations for Expression Systems :
201 - 250 of 358 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... for 60-72 hrs maintained in ESF 921 Insect Cell Culture Medium (Expression Systems) at 27°C with ambient CO2 and gentle agitation ...
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bioRxiv - Biophysics 2022Quote: ... The protein was expressed in Trichoplusia ni ovarian cells (Expression Systems) for 60-72 hrs maintained in ESF 921 Insect Cell Culture Medium (Expression Systems ...
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bioRxiv - Biophysics 2022Quote: ... P0 virus was produced by transfection with FuGENE HD and BestBac 1.0 Linearized Baculovirus DNA (Expression Systems) followed by a viral amplification to P1 ...
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bioRxiv - Immunology 2022Quote: ... 2 μg of plasmid DNA was transfected into SF9 insect cells using BestBac 2.0 baculovirus DNA (Expression Systems; Davis, CA) and Cellfectin II reagent (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: Drosophila Schneider 2 (S2) cells (Expression Systems, 94-005F) were cultured in ESF media (ESF 921 Insect Cell Culture Medium ...
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bioRxiv - Biochemistry 2022Quote: ... were cultured in ESF media (ESF 921 Insect Cell Culture Medium, Expression Systems) with 5% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: SF9 cells growing in ESF921 media (Expression Systems) were co-transfected with linearized viral genome and the expression plasmid and selected for high infectivity ...
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bioRxiv - Biochemistry 2022Quote: ... Cell cultures were grown in ESF 921 serum-free medium (Expression Systems) to a density of 2.5 × 106 cells/mL and then infected with the UBR5 baculovirus (3%-5% v/v ...
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bioRxiv - Biochemistry 2022Quote: ... Sf9 cell cultures were grown to cell density at about 4×106 cells/ mL in ESF 921 serum-free medium (Expression Systems). Subsequently ...
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bioRxiv - Biochemistry 2022Quote: ... Isolated DNA was then transfected into Sf9 cells (Expression Systems, 94-001) via Polyethylenimine PEI 25K (Polysciences ...
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bioRxiv - Biochemistry 2022Quote: ... and analysis of the recombinant bacmid were performed following the manufacturer’s instructions (ExpiSf Expression System, Gibco). ExpiSf9 cells were cultured in ExpiSf9 CD medium to a density of 5×106 cells/mL ...
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bioRxiv - Biochemistry 2022Quote: ... When the cells grew to a density of 3.5 × 106 cells/mL in ESF 921 cell culture medium (Expression Systems), we infected the cells with five separate virus preparations at a ratio of 1:2:2:2:2 for PTH1R(27-502)-LgBiT-2MBP ...
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bioRxiv - Biochemistry 2022Quote: ... Cell cultures were grown in ESF 921 medium (Expression Systems) to a density of 3 × 106 cell/mL and then infected with the different types of baculoviruses ...
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bioRxiv - Biochemistry 2022Quote: ... High Five cells (Expression Systems) in suspension culture at a density of 1×106 cells/ml were infected with baculovirus (V1 ...
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bioRxiv - Biochemistry 2022Quote: ... Baculoviruses for respective constructs were produced by transfecting Sf9-cells (Expression Systems) with FuGENE HD (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... Omicron-SARS-CoV-2 spike RBD and ACE2 variants (CVD293 and CVD432) were expressed in high density Expi293FTM cells in Expi293TM expression media following manufacturer’s protocol (Expi293 Expression System, ThermoFisher Scientific). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... human full-length CB1 containing a N-terminal FLAG tag and C-terminal histidine tag was expressed in Spodoptera frugiperda Sf9 insect cells with the baculovirus method (Expression Systems). Receptor was extracted using 1% lauryl maltose neopentyl glycol (L-MNG ...
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bioRxiv - Biochemistry 2022Quote: ... 40 mL of a Sf9 suspension culture were prepared in ESF921 medium (Expression Systems) to a density of ∼1.5 M/mL ...
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bioRxiv - Biochemistry 2022Quote: ... Spodoptera frugiperda Sf9 insect cells were co-transfected with the constructed plasmids and linearized baculovirus DNA (Expression Systems, 91-002) using Cellfectin II (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: ... or in ESF medium (Expression Systems) at 27°C ...
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bioRxiv - Cancer Biology 2022Quote: ... larvae were bled in ice-cold ESF921 media (Expression Systems, Cat# 500302) and the hemolymph from one larva was transferred to a corresponding well in a black 384-well glass-bottom plate (CellVis ...
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bioRxiv - Biophysics 2022Quote: ... EEA1 was expressed in SF9 cells growing in ESF921 media (Expression Systems) as specified in Supplementary Information Sec ...
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bioRxiv - Biophysics 2022Quote: ... Sf9 cells were cultured in ESF 921 medium (Expression Systems, Davis, CA) and P1 virus was generated from cells transfected with Escort IV reagent (MillaporeSigma ...
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bioRxiv - Immunology 2022Quote: ... 500 ml SF9 cells (∼106 cells/ml; Expression Systems, 94-001F) were infected with high-titer baculoviral supernatant ...
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bioRxiv - Immunology 2022Quote: ... and subsequently grown for 72 h at 27 °C and 85 rpm in ESF 921 culture medium (Expression Systems, 96-001-01). Cell pellets were obtained by centrifugation at 1000 rcf for 5 min and routinely flash frozen in liquid nitrogen (−196 °C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Tni and Sf9 cells (Expression Systems) were maintained in ESF-921 media (Expression Systems ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... were maintained in ESF-921 media (Expression Systems) at 27°C.
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bioRxiv - Pharmacology and Toxicology 2022Quote: PfMyoA was expressed as described previously [17] in Sf9 cells adapted to serum-free ESF 921 media purchased from Expression Systems. Sf9 cells were co-infected with PfMyoA ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were kept in ESF 921 Insect Cell Culture Medium (Expression systems) that was supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... were co-expressed in SF9 insect cells in ESF921 medium (Expression Systems), in the presence of 50 µM zinc acetate ...
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bioRxiv - Cell Biology 2022Quote: SF9 cells growing in ESF921 media (Expression Systems) were co-transfected with linearized viral genome and the expression plasmid ...
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bioRxiv - Physiology 2022Quote: ... 2X TaqMan Universal Master Mix (Applied Biosystems, TaqMan® Gene Expression Systems), and cDNA template ...
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bioRxiv - Biophysics 2022Quote: ... Protein Free (Expression Systems) and seeded in a 6-well plate to produce baculovirus ...
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bioRxiv - Biophysics 2022Quote: ... Sf9 cells were grown in ESF921 media (Expression Systems) at 27 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Trichoplusia ni Hi5 insect cells (unauthenticated and untested for mycoplasma contamination, Expression Systems) were infected at a density of 3.0 x 106 cells/mL with high titer Gβ1γ2-baculovirus ...
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bioRxiv - Biochemistry 2022Quote: ... in Spodoptera frugiperda Sf9 insect cells (unauthenticated and untested for mycoplasma contamination, Expression Systems). For expression ...
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bioRxiv - Biophysics 2022Quote: ... Viral titers were performed by the flow cytometric method23 on a Guava easycyte 8HT in which cells were stained with Anti-gp64-PE antibody (Expression Systems).
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bioRxiv - Biophysics 2021Quote: ... Sf9 cells were seeded at 2 x 106 cells / mL in ESF-921 (Expression Systems), and infected simultaneously with myosin-15 baculovirus at a multiplicity of infection (MOI ...
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bioRxiv - Biophysics 2021Quote: ... in sf9 insect cells (Expression Systems). High Five™ cells (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... The protein was expressed in 2 L of Sf9 cells at 2×106 cells/mL maintained in ESF921 media (Expression Systems) by adding 25 mL virus per liter of culture ...
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bioRxiv - Immunology 2021Quote: ... along with BestBac 2.0 v-cath/chiA Deleted Linearized Baculovirus DNA (Expression Systems) for 6 hours ...
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bioRxiv - Genetics 2021Quote: ... ESF921-adapted S2 cells (Expression Systems, 94-005S) were transfected using PEI transfection methods and cultured in protein-free ESF921 media (Expression Systems ...
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bioRxiv - Genetics 2021Quote: ... were transfected using PEI transfection methods and cultured in protein-free ESF921 media (Expression Systems, 96-001). For 6-well plate transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Subsequent steps used Spodopetera frugiperda SF9 cells cultured in ESF 921 medium (Expression Systems, Davis, CA). Bacmid was transfected into adherent SF9 cells using the Escort IV (MillaporeSigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell cultures were grown to a density of 4×106 cells/ mL in ESF 921 serum-free medium (Expression Systems). For the expression of the DRD1-Gs complexes ...
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bioRxiv - Molecular Biology 2021Quote: ... Trichoplusia ni High Five cells (Expression Systems) were infected (or coinfected ...
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bioRxiv - Molecular Biology 2021Quote: Spodoptera frugiperda (Sf9) insect cells (Expression Systems) were grown in ESF 921 serum-free medium (Expression Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... were grown in ESF 921 serum-free medium (Expression Systems) at 27°C and 120 rpm ...
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bioRxiv - Immunology 2021Quote: ... GenBank: MN908947.3) under the control of a tetracycline-inducible promoter (Retro-X™ Tet-One™ Inducible Expression System, Takarabio) with a puromycin selection marker ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: ... were cultured in ESF 921 serum-free medium (Expression Systems) at 27°C and 120 rpm ...