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Citations for Electron Microscopy Sciences :
401 - 450 of 475 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... slides were washed twice with 1 mL of PBS wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Cell Biology 2023Quote: ... The diluted sperm solution was mixed at a ratio of 1:1with 10 X concentrated 10 nm protein A-coated gold fiducials (Electron Microscopy Sciences). 2.7 μL of the mixture was applied onto the grids and blotted for 1.5 to 2.5 s in 100% relative humidity and 4 °C ...
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bioRxiv - Bioengineering 2024Quote: 10 μL of samples with an optimized concentration (1.0∼1.5 x 1010 /100 μL range) in double-filtered PBS solution were deposited onto the TEM grid (Formvar/Carbon 300 Mesh, Copper; #FCF300-CU, Electron Microscopy Sciences) and incubated for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... stained with Hoechst dye according to the manufacturer’s specifications and then overlayed with CitiFluor Mountant AF1 Solution (Electron Microscopy Sciences, Hatfield, PA).
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bioRxiv - Neuroscience 2024Quote: ... iPS cell-derived neuronal cultures were fixed using 4% paraformaldehyde diluted in 1 PBS from a 16% aqueous solution (Electron Microscopy Sciences) for 20 min and then permeabilized with 0.2% Triton X-100 (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: For wholemount immunofluorescent labeling heads were bisected and brains removed prior to immersion fixation of the intact inner ear and skull in 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences) prepared in 67mM Sorensons phosphate buffer (pH 7.4) ...
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bioRxiv - Synthetic Biology 2023Quote: ... then fixed for 10 min with pre-warmed 4% Paraformaldehyde solution (37°C; prepared from 16% PFA (Electron Microscopy Sciences and DPBS) at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: Primed and naïve hESCs were prepared as described in the above section and fixed for 10 min in a 3% paraformaldehyde solution (Electron Microscopy Science). Cells were then permeabilized for 7 min with ice-cold PBS supplemented with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2023Quote: ... and sections were heat-mounted to glass slides and stained with a diluted toluidine blue solution (catalog no. 14950, Electron Microscopy Sciences).
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bioRxiv - Neuroscience 2023Quote: Mice were anesthetized and perfused with normal Ringer’s solution at 35°C followed by 0.15M cacodylate buffer containing 4% formaldehyde (Electron Microscopy Sciences, Hartfield, PA). Brain tissue was cut into 100 μm vibratome sections ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were washed five times for 10 min each with sterile deionized water and then transferred to fresh solution containing 1% (w/v) thiocarbohydrazide (EMS, Cat# 21900) in deionized water and incubated for 1 hour under rotation at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Labella were dissected from 6—8 day-old flies and fixed in a solution containing 4% paraformaldehyde (Electron Microscopy Sciences, Cat # 15710) and 0.2% Triton X-100 for 15 minutes at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... egg-PC/ Sphingomyelin (3:1 molar ratio)} solutions were applied to a thin film of carbon-coated copper grids (Carbon film 300 mesh, Copper, Electron Microscopy Sciences) at room temperature ...
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bioRxiv - Pathology 2024Quote: ... then incubated overnight at room temperature in LFB solution (0.1% in 95% alcohol with acetic acid, Electron Microscopy Sciences, Hatfield, PA, USA). In groups of 3 or 4 with at least one normal sample ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of diluted phage solution was applied onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA, USA). Following a 3-minute incubation ...
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bioRxiv - Developmental Biology 2024Quote: ... The leg fragments were then placed directly into 500 µl of 4% paraformaldehyde (20% EM grade stock solution, Electron Microscopy Sciences #15713) in filtered artificial seawater for 50 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... cells grown on coverslips were immediately chemically crosslinked for 15 minutes with 4% PFA diluted from a 16% stock solution (Electron Microscopy Sciences, 15710) with 1x PHEM (60 mM PIPES ...
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bioRxiv - Neuroscience 2021Quote: ... the astrocytes were washed with DPBS and then fixed with 4% paraformaldehyde (diluted from a 16% solution; Electron Microscopy Sciences cat. no. 15710) for 15 min at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μL of sample solution was applied onto a glow-discharged 300-mesh copper grid coated with continuous carbon film (EMS, Hatfield, PA, USA). The sample solution was left for 30 s before blotted with a piece of filter paper ...
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bioRxiv - Bioengineering 2020Quote: ... kept in ice and then they incubated for 2h in ice and in the dark in a freshly prepared staining solution (SSI) made of 1% OsO4 (Electron Microscopy Sciences, 19190), 1.25% potassium hexacyanoferrate (II ...
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bioRxiv - Cell Biology 2021Quote: ... cells grown on coverslips were immediately chemically crosslinked for 15 minutes with 4% PFA diluted from a 16% stock solution (Electron Microscopy Sciences, 15710) with 1x PHEM (60 mM PIPES ...
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Cobalt(III) Schiff Base complexes stabilize non-fibrillar amyloid-β aggregates with reduced toxicitybioRxiv - Neuroscience 2020Quote: ... Aggregated solutions were diluted to 10 μM and 10 μl was spotted onto formvar/carbon coated nickel grids (Electron Microscopy Sciences, Hatfield, PA) for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: The cells of interest were washed three times in PBS pH 7.2 and fixed in 2.5% glutaraldehyde solution grade I (Electron Microscopy Sciences, Hatfield, PA, USA) in sodium cacodylate buffer 0.1 M pH 7.2 for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Ovaries of the female mice that were in the fertility trials were fixed in Modified Davidsons fixative solution (Electron Microscopy Sciences, #6413-50) for 6–12 h and were processed by the Office of Translational Science at Rutgers University for histology services ...
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bioRxiv - Biophysics 2022Quote: ... The grids were then stained with 50 μL aqueous uranyl acetate solution (2% v/v) (Electron Microscopy Sciences, Philadelphia, USA. Product No: 22400) for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... At that time cells were washed once with sterile 1x PBS and then fixed with 4% PFA solution (Electron Microscopy Sciences; Ref# 15710) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1x PBS (∼5-7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1x PBS (∼5-10 ml ...
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bioRxiv - Biophysics 2021Quote: Neural cell cultures were fixed post-live-cell fluorescent imaging using a solution of 4% v/v paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) + 8% w/v sucrose in 1× PBS for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... The tethered EVs were fixed in a 2 % glutaraldehyde (MilliporeSigma, Burlington, MA) and 0.1 M sodium cacodylate solution (Electron Microscopy Sciences, Hatfield, PA) for 3 hr ...
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bioRxiv - Microbiology 2024Quote: ... the wells were fixed by adding on top of the supernatant 10% formaldehyde (Formaldehyde Aqueous Solution EM grade, 32%, Liquid Electron Microscopy Sciences, # 15700) at a volume-to-volume ratio for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: 5 μl of PFF solution (∼1 mg/mL) was spotted on a freshly glow-discharged 300-mesh carbon coated formvar grid (Electron Microscopy Sciences, USA) and incubated for 3 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Acute slices were washed briefly with PBS to remove excess ACSF solution then transferred to a 4% PFA (Electron Microscopy Science Cat# 15714) in PBS solution in 4°C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... VLP samples were fixed with 2% (v/v) glutaraldehyde in water solution on 400 mesh Formvar-coated copper grids (EMS Cat#FF400-Cu). After fixation ...
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bioRxiv - Cancer Biology 2023Quote: 3.105 cells were seeded onto glass slides and fixed for 12min at room temperature with a solution of 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15710) diluted in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... dehydrated in a graded series of ethanol ranging from 30% to absolute solution and embedded in resin (EMBed-812, Electron Microscopy Sciences, USA). 80 nm thick sections from at least four blocks from Ctr iPSC-derived MYOrganoids in presence or absence of Ctr fibroblasts were stained with uranyl acetate and lead citrate ...
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bioRxiv - Cell Biology 2024Quote: ... The filters were then removed from the culture dishes and incubated overnight in 4% paraformaldehyde solution at 4 °C (Electron Microscopy Sciences #15713). In preparation for paraffin embedding and sectioning ...
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bioRxiv - Neuroscience 2024Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1X PBS (∼5–7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1X PBS (∼5–10 ml ...
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bioRxiv - Neuroscience 2024Quote: Three weeks after cervical AAV injection and one to fourteen days after sham or intracortical injury animals underwent transcardial perfusion with 0.9% saline and 4% paraformaldehyde (PFA) solutions in 1× phosphate-buffered saline (PBS) (15710, Electron Microscopy Sciences, Hatfield, PA). Whole brains were dissected and fixed overnight in 4% PFA at 4°C and washed three times in PBS pH 7.4 ...
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bioRxiv - Physiology 2024Quote: ... Hearts were perfused for 3 min with PBS at 37°C to wash out the blood followed by a fixative solution of Glutaraldehyde 3% in 0.1M Cacodylate buffer pH7.4 (Electron Microscopy Sciences-cat#16538-15) for less than 5 min ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were initially washed with 1X Assay buffer (GOLGI-ID kit) and then fixed with 4% of 16% paraformaldehyde solution (Electron Microscopy Sciences, 15710) at room temperature for 10 minutes before staining the cells for detection of Golgi bodies using the manufacture’s manual for “Staining Adherent Fixed Cells” ...
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bioRxiv - Biophysics 2024Quote: Cryo-EM samples of concentrated peptide solutions were prepared on either 300 mesh 2/2 Quantifoil or C-flat EM grids (Electron Microscopy Sciences, USA), on which 10 nm protein A-conjugated colloidal gold particles (Au–NP ...
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bioRxiv - Cell Biology 2024Quote: Primary neurons or astrocytes cultured on glass coverslips at the same plating density as was used for live cell imaging experiments were fixed for 10 minutes at 37°C/5% CO2 in a solution containing 2% paraformaldehyde (PFA; Electron Microscopy Sciences 15713) and 0.05% glutaraldehyde in PHEM buffer (EMS ...
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bioRxiv - Immunology 2021Quote: ... was added to anti-CD16/32 solution at a 1 : 100 dilution for dead cell exclusion and fixed with 4% paraformaldehyde (15710, Electron Microscopy Sciences, Lucerne, Switzerland) in PBS for 20 min on ice after staining.
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bioRxiv - Neuroscience 2022Quote: ... specimens were dehydrated using serially graded ethanol solutions (30%, 50%, 70%, 90% and 100%) and infiltrated and embedded in Spurs resin (Electron Microscopy Sciences, Hatfield, PA) following the manufacturer’s recommendations ...
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bioRxiv - Systems Biology 2022Quote: ... 6F- Volume was completed to 8 ml with room temperature 1 X PBS and then 500 μl of 16 % PFA solution (Electron Microscopy Sciences, cat. # 15710) was added ...
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bioRxiv - Cell Biology 2021Quote: ... The sections are again washed thrice in PBS and stained with DAPI for 5 minutes and mounted with Fluoromount-G solution (17984-25, Electron Microscopy Sciences, Pennsylvania, USA). Using a Zeiss Axio Imager Z1 ApoTome Microscope ...
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bioRxiv - Neuroscience 2021Quote: ... and tran-scardially perfused with 20ml of 0.01M phosphate buffered saline (1X PBS) followed by 20ml of 4% paraformaldehyde (diluted from a 32% aqueous paraformaldehyde solution with PBS; Electron Microscopy Sciences [EMS], Hatfield, Pennsylvania) at a flow rate of 10-14ml/min ...
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bioRxiv - Neuroscience 2023Quote: ... rats were transcardially perfused with an isotonic solution at 9.25% in distilled water as a prewash and 4% paraformaldehyde (PFA; fixative, Cat No. 15714-S, Electron Microscopy Sciences, Hatfield, PA, USA) using the Perfusion Two TM Automated Pressure Perfusion System (Leica Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with heparin (12.5 U/ml) followed by 25 ml RT fixative solution consisting of 4% PFA and 2.5% glutaraldehyde (both Electron Microscopy Science, Hatfield, PA, USA) in PBS at a flow rate of 2 ml/min ...