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Citations for Electron Microscopy Sciences :
351 - 400 of 475 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... 5 μL of the diluted DNA solution was dropped onto a carbon film coated TEM grid (Electron Microscopy Sciences, PA, USA), and incubated at room temperature for 1.5 min ...
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bioRxiv - Cell Biology 2020Quote: ... The slices were then transferred to capped glass vials and postfixed with 1% osmium tetroxide diluted in PBS from a 4% aqueous solution (EMS 19170) for 30 minutes on a rotator ...
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bioRxiv - Developmental Biology 2020Quote: ... Then the samples were dehydrated in sequential ethanol solutions and embedded in an Eponate mixture (Electron Microscopy Sciences, Hatfield, PA, USA) for polymerization for 24h at 60°C ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... or FHIP1B KO stably expressing FHIP1B-TagRFPT-V5) were grown on fibronectin coated glass coverslips and fixed with a solution of 3.7% formaldehyde (Electron Microscopy Sciences), 90% Methanol (VWR) ...
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bioRxiv - Biochemistry 2020Quote: ... 4μL of the resulting solution was deposited on glow-discharged Quantifoil grids (R2/1, Cu 200-mesh grid, Electron Microscopy Sciences). The solution on the grid was then blotted using a Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences. 2,2’-Bipyridine 99+% (bipy ...
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bioRxiv - Biophysics 2022Quote: ... neurons were rinsed with sterile Hank’s Balanced Salt Solution and fixed for 10min with pre-warmed 4% PFA (Electron Microscopy Sciences) in Phosphate Buffered Saline (PBS) ...
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bioRxiv - Microbiology 2022Quote: ... salivary glands collected from infected mosquitos at day 27 post-infection were fixed in a solution of 2,5% glutaraldehyde (Electron Microscopy sciences) with 2% formaldehyde (Electron Microscopy sciences ...
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bioRxiv - Microbiology 2021Quote: ... Monolayers were fixed and plaques visualized with a solution of 0.4% crystal violet by weight in 80% methanol (MilliporeSigma) and 4% PFA (Electron Microscopy Sciences) for 20 min at room-temperature (RT).
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bioRxiv - Immunology 2020Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 300 mesh formvar-carbon-coated copper grids and uranyl acetate replacement solution (UAR) were purchased from Electron Microscopy Sciences (Hatfeild, PA).
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bioRxiv - Physiology 2021Quote: The mouse liver was cut into 2-3 mm3 cubes and immediately immersed in a fixation solution containing 2.5% glutaraldehyde (Electron Microscopy Science) in 0.1 M cacodylate buffer pH 7.4 for 1 hour at room temperature and then overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL protein solution at 0.01 mg/mL was added to a glow-discharged carbon-coated copper grid (Electron Microscopy Science). Grids were stained in 2% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were rinsed three times at five-minute intervals with deionized ultrapure water followed by a 30-minute incubation at RT with a filtered (Whatman, 0.2 μm) freshly prepared solution of 1% thiocarbohydrazide (Electron Microscopy Sciences) made by dissolving it at 60°C for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... followed by staining in Sato’s lead (a solution of 1% lead acetate, 1% lead nitrate,and 1% lead citrate; all from Electron Microscopy Sciences) for 2 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5 µL of diluted phage solution was dropped onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA). After 3 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed thoroughly in 0.1M PBS, followed by a series of washes in Enhancement Conditioning Solution (1X concentration per package instructions, Electron Microscopy Science (EMS), Cat 25830 ...
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bioRxiv - Immunology 2024Quote: ... slides were washed twice for 5 min in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low- barium PBS for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... and a 50% resin solution (24 g Embed 812, 9 g DDSA, 15 g NMA, 1.2 g BDMA; 14121; Electron Microscopy Sciences) in propylene oxide ...
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bioRxiv - Cancer Biology 2024Quote: ... and pellets were resuspended in a 5 ml volume of fixative master mix of Paraformaldehyde-Glutaraldehyde Solution Karnovsky’s Fixative (Electron Microscopy Sciences). Fixative solution was prepared with 16% paraformaldehyde solution ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were rinsed thoroughly in 0.1M PBS, followed by a series of washes in Enhancement Conditioning Solution (1X concentration per package instructions, Electron Microscopy Science (EMS), Cat 25830 ...
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bioRxiv - Systems Biology 2023Quote: ... slides were washed twice for 5 minutes in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... they were counterstained with uranyl acetate and Reynold’s lead citrate (Reynolds, 1963) or uranyl acetate replacement solution (EMS, Science Services, USA). Micrographs of both hair cell types from the striolar and extrastriolar region were acquired at 10,000-x magnification with a Gatan Orius 1200A camera (Gatan GmbH ...
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bioRxiv - Biophysics 2023Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences, and 2,2’-Bipyridine 99+% (bipy ...
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bioRxiv - Cell Biology 2023Quote: Neurons plated on coverslips were rinsed with sterile Hank’s Balanced Salt Solution (HBSS) and fixed for 10min with pre-warmed 4% paraformaldehyde (PFA) (Electron Microscopy Sciences) in phosphate buffered saline (PBS) ...
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bioRxiv - Biophysics 2023Quote: ... they were fixed for 90 min at room temperature with 4% PFA/2% Sucrose solution supplemented with 0.1% Glutaraldehyde (GA, Electron Microscopy Sciences), washed five times with PBS ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of diluted phage solution was dropped onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA). After 3 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The grid was washed with MQ water and stained for 20 min with 10 μl 0.1% uranyl formate solution (Electron Microscopy Sciences). The uranyl formate solution was prepared freshly and filtered with a 0.22 μm sterile syringe filter (Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... The sample was prepared as follows: 1 µL of ∼0.1 nM nanoparticles solution was left to dry onto a TEM grid (CF300-Cu, Electron Microscopy Sciences) for 30 min ...
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Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomographybioRxiv - Cell Biology 2023Quote: ... followed by staining in Sato’s lead (a solution of 1% lead acetate, 1% lead nitrate, and 1% lead citrate; all from Electron Microscopy Sciences) for 2 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... A 5 μL drop of the solution was deposited onto a glow-discharged formvar/carbon-coated copper grid (Electron Microscopy Sciences). The grid was incubated for 2 minutes ...
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bioRxiv - Biophysics 2024Quote: ... grids containing sections were incubated (face down) on 100 μL drops of Aurion Blocking Solution (Electron Microscopy Sciences, Hatfield, PA, USA) for 1h at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... A small drop (2.5 mL) of the protein solution was applied to the carbon side of a glow-discharged grid (EMS, CF300-CU) and left on the grid for 1 min ...
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bioRxiv - Cell Biology 2024Quote: ... mexicana were fixed directly in medium for 10 min at room temperature in 2.5% glutaraldehyde (glutaraldehyde 25% stock solution, EM grade, Electron Microscopy Sciences). Centrifugation was carried out at room temperature for 5 min at 16,000 g ...
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bioRxiv - Cell Biology 2024Quote: Cells were first fixed 1.5h at room temperature with 2x fixation solution (5% glutaraldehyde and 4% paraformaldehyde (EMS, Delta-Microscopies, France) in 0.1 M Sorensen buffer ...
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bioRxiv - Immunology 2024Quote: ... Sections were fixed in 10% formalin for 30 minutes at room temperature and incubated in Bouin’s fixative solution (Electron Microscopy Sciences) overnight at room temperature ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was added to immobilize rosettes (300 µL of a 1:2,000 dilution of a 16% PFA stock solution; Electron Microscopy Sciences 15714 ...
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bioRxiv - Neuroscience 2024Quote: ... transcardially perfused with PBS for 5 min and then with 10 mL of 4% PFA solution (Electron Microscopy Sciences, Cat#15714) for immunohistochemistry or with 10 mL of 2.5% glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... were mixed with 0.5 µl of 0.04 mM Lauryl maltose neopentyl glycol (LMNG) solution immediately before sample deposition onto QUANTIFOIL® 2/1 400 mesh grids (EMS), which were plasma cleaned for 10 s using Gatan Solarus 950 Plasma system (Ar/O2 gas mixture) ...
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bioRxiv - Immunology 2024Quote: ... slides were washed twice for 5 min in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... followed by staining in Sato’s lead (a solution of 1% lead acetate, 1% lead nitrate, and 1% lead citrate; all from Electron Microscopy Sciences) for 2 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Uranyl Acetate (1% solution, 22400-1) and TEM grids (Carbon support film on 400 mesh copper, CF400-CU) were purchased from Electron Microscopy Sciences.
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bioRxiv - Immunology 2021Quote: ... EM grids were prepared by staining 1:20 and 1:100 dilutions (in PBS plus 250 mM NaCl) of lyophilised and resuspended sample with 2 % uranyl formate solution on carbon-coated CF400-CU-UL grids (Electron Microscopy Sciences) as described earlier ...
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bioRxiv - Cell Biology 2022Quote: ... then fixed for 10 min at room temperature with pre-warmed 4% Paraformaldehyde solution in DPBS (37°C; prepared from 16% PFA (Electron Microscopy Sciences)) ...
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bioRxiv - Cell Biology 2021Quote: Mice were sacrificed by cervical dislocation and sciatic nerve were exposed and fixed by adding fixative solution (2% PFA (15710, Electron Microscopy Sciences), 2.5% Glutaraldehyde (16220 ...
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bioRxiv - Bioengineering 2020Quote: ... 10 μl of the particle solutions were dropped on the 200 mesh copper grid covered with a carbon-stabilized Formvar film (Electron Microscopy Sciences). The residual solution was removed after 5 min ...
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bioRxiv - Microbiology 2021Quote: ... of 0.01 and cultured in 6-well plates for 48 h with cMEM or cells from ETAs of COVID-19-infected patients were fixed with a freshly prepared 2% or 4% PFA solution (20% stock diluted in 1X HBSS; Electron Microscopy Sciences) or a 1.6X BD FACS™ Lysis Solution (1:6 in sterile dH2O ...
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bioRxiv - Physiology 2021Quote: ... whole heads or enucleated eyes were fixed for 20 minutes on ice in 4% PFA prepared in PBS from 20% stock solution (15713, Electron Microscopy Sciences). The samples were rinsed with PBS and stored in 100% methanol at −20°C ...
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bioRxiv - Neuroscience 2021Quote: ... the patch pipette was gently removed from the recorded PC and the slice was immediately transferred from the recording chamber to a fixation solution composed of 4% paraformaldehyde (Electron Microscopy Sciences) in ACSF for a maximum of 24h (4°C).