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Citations for Electron Microscopy Sciences :
451 - 475 of 475 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... and pancreas were harvested at the experimental endpoint and immediately fixed in 4% paraformaldehyde in 1X PBS (16% solution, Electron Microscopy Sciences, cat# 15711) at 4°C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... Slides were washed twice for 5 min in wash buffer and fixed for 5 min in diluted glutaraldehyde solution 2% (Electron Microscopy Sciences, Hatfield, PA) in PBS-low barium (Ionpath ...
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bioRxiv - Cell Biology 2022Quote: Four μm kidney cortices optimal cutting temperature (OCT) compound embedded sections were incubated with 100 μl freshly prepared Oil Red O solution (Electron Microscopy Science, Hatfield, PA) for 15 minutes and counterstained with Hematoxylin Harris solution (VWR ...
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bioRxiv - Neuroscience 2024Quote: ... Cat# 70011044) and fixation with 4% paraformaldehyde (PFA) (dilution 1:4; Paraformaldehyde 16% Aqueous Solution EM Grade, Electron Microscopy Sciences, Hatfield, PA; Cat# 15710) were performed 6 hours after saline or LPS injection ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated in a 2% OsO4 in 0.15 M cacodylate buffer solution containing 2 mM calcium chloride (19152, Electron Microscopy Sciences, Hatfield, PA, USA) for 2 hours in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... A 3 µL droplet of the D-GQD solution (5 µM) was placed on the carbon-coated copper TEM grid (Purchased from Electron Microscopy Sciences; PA, USA), and air-dried ...
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bioRxiv - Cell Biology 2024Quote: ... fixed for 20 min in 4% EM-grade paraformaldehyde (PFA) diluted in phosphate-buffered saline (PBS) from an 8% PFA Aqueous Solution (Electron Microscopy Sciences, 157-8) and blocked for 1 h in PBS plus 0.2% Triton X-100 and 4% normal goat serum (NGS ...
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bioRxiv - Biophysics 2020Quote: ... 5 uL of DNA origami solution (5 nM) was deposited onto fresh glow discharged carbon film with copper grids (CF200H-CU; Electron Microscopy Sciences Inc., Hatfeld, PA), and the sample was then allowed to absorb onto the surface for 30s ...
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bioRxiv - Genetics 2020Quote: ... Then the cells were counted and diluted to 106 cells/ml and fixed with 4% paraformaldehyde aqueous solution (Electron Microscopy Sciences #15710, Formaldehyde methanol-free) for 10 minutes at room temperature (RT) ...
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bioRxiv - Neuroscience 2022Quote: Postnatal mouse were transcardially perfused with 15ml (P14) or 25ml (>P21) of 2% PFA freshly prepared from 32% PFA solution (Electron Microscopy Sciences, 50-980-495). Perfused brains were dissected out ...
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bioRxiv - Developmental Biology 2021Quote: ... The eye imaginal discs were dissected in cold 1X solution of Phosphate Buffered Saline (PBS) and fixed using 4% p-formaldehyde (Electron Microscopy Sciences, Catalog no. 15710). Fixed tissues were washed using PBS-T [0.2% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... the cultured neurons were fixed for 10-15 min by transferal of the coverslip into a dish with 4% paraformaldehyde (16% aqueous solution, 15710; Electron Microscopy Sciences, Hatfield, Pennsylvania, USA) and 0.2% glutaraldehyde (25% aqueous solution ...
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bioRxiv - Bioengineering 2020Quote: Animals were implanted with a stainless-steel probe or a Fiber F1/F2 for 4 weeks and then anesthetized with a ketamine (20 mg/mL)/xylazine (2 mg/mL) solution and transcardially perfused with phosphate-buffered saline (PBS) (Fisher BP661-10) followed by 4% paraformaldehyde (PFA) (Electron Microscopy Sciences Cat. #15714-S) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... and tran-scardially perfused with 20ml of 0.01M phosphate buffered saline (1X PBS) followed by 20ml of 4% paraformaldehyde (diluted from a 32% aqueous paraformaldehyde solution with PBS; Electron Microscopy Sciences [EMS], Hatfield, Pennsylvania) at a flow rate of 10-14ml/min ...
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bioRxiv - Neuroscience 2021Quote: ... cultures were washed in imaging buffer to remove non-internalised HRP and fixed with a solution of 2 % glutaraldehyde (Electron Microscopy Sciences, Hatfield, USA, 16019) in phosphate buffered saline ...
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bioRxiv - Neuroscience 2023Quote: ... cultures were washed in imaging buffer to remove non-internalised HRP and fixed with a solution of 2 % glutaraldehyde (Electron Microscopy Sciences, Hatfield, USA, 16019) and 2% PFA in 0.1 M in phosphate buffer (PB) ...
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bioRxiv - Neuroscience 2023Quote: ... the coverslips were rinsed with 1X PBS and then fixed with freshly prepared 4% paraformaldehyde (from 32% Paraformaldehyde Solution, Electron Microscopy Science Cat# 15714-S) for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... A portion of each EV sample (10 μl) was fixed in a 1:1 solution using 4% EM-grade paraformaldehyde (Electron Microscopy Sciences, 157-4-100) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The 2 ml of EMS supplemented M9 buffer was added to the 2 ml worm suspension (final concentration of EMS in solution is ∼50 mM). The worms were incubated for 4 hours at room temperature constantly mixing on a nutator ...
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bioRxiv - Neuroscience 2024Quote: mice were perfused 7 dpI and 10 dpl with 25 mL of 2% PBS-paraformaldehyde freshly prepared from 32% PFA solution (Electron Microscopy Sciences, 50-980-495). Perfused brains were dissected out ...
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bioRxiv - Developmental Biology 2022Quote: ... with commercial antigen unmasking solution (VECTOR, Catalog #: H-3300) and underwent immunostaining procedure using the Sequenza™ Immunostaining Center System (Electron Microscopy Sciences, Catalog #: 71407-01)(Zhao et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The midbrain and eyestalk neural tissue was dissected free from the exoskeleton and its enveloping sheath in ice-cold chromating solution comprising 1 part 25% glutaraldehyde (Electron Microscopy Sciences; Cat# 16220; Hatfield, PA), 5 parts 2.5% potassium dichromate (Sigma Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... 50 uL of the gelation solution was added to the samples before immediately transferring them to silicone incubation chambers (Electron Microscopy Sciences, Cat. No. 70324-05) and sealing with a glass microscope slide ...
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bioRxiv - Neuroscience 2020Quote: ... and then deeply anesthetized by Fatal-plus injection (0.075 mL of 97.5 mg/mL solution in NaCl) followed by a standard transcardial perfusion using PBS (50 mL) followed by PFA (Electron Microscopy Sciences, 50 mL, 4% in PBS).
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and the pellet was fixed in 2.5% glutaraldehyde and 4% PFA (16% stock solution, EM grade, Electron Microscopy Sciences in 0.1 M PIPES (pH 7.2)) for minimum 2h ...