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Citations for Electron Microscopy Sciences :
251 - 300 of 1193 citations for 1 3 Fluorophenyl 5 oxopyrrolidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and 3 µL of the complex/detergent mixture was immediately applied to QuantiFoil 300 mesh 1.2/1.3 grids (Electron Microscopy Sciences, Hatfield, PA) that had been freshly glow discharged with PELCO easiGLOW (Ted Pella ...
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bioRxiv - Microbiology 2024Quote: ... amylovora strain Ea110 were collected at 3 dpi and fixed in paraformaldehyde/ glutaraldehyde (2.5% of each compound in 0.1 M sodium cacodylate buffer) (Electron Microscopy Sciences, Hatfield, PA) at 25 C overnight ...
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Protein identification using cryo-EM and artificial intelligence guides improved sample purificationbioRxiv - Biochemistry 2024Quote: ... 3 µL of the diluted sample was applied to a freshly glow discharged 400 carbon square mesh grid (Electron Microscopy Sciences, EMS) and allowed to adsorb onto the grid for 30 seconds ...
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Protein identification using cryo-EM and artificial intelligence guides improved sample purificationbioRxiv - Biochemistry 2024Quote: ... 3 µL of the diluted sample was applied to a freshly glow discharged 400 carbon square mesh grid (Electron Microscopy Sciences, EMS) and allowed to adsorb onto the grid for 30 seconds ...
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bioRxiv - Cell Biology 2021Quote: ... fixed for 5 min with 1% glutaraldehyde (Electron Microscopy Sciences).
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: Flow chambers were created using tape placed ~3 mm apart on a glass slide to adhere coverslips coated with 0.1% collodion in amyl acetate (Electron Microscopy Sciences, Hatfield, PA). For standard gliding assays with β-cardiac myosin eGFP ...
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bioRxiv - Cell Biology 2023Quote: ... astrocytes loaded with either unlabeled or AF647 NHS ester-labeled tau fibrils (0N4R PFF, described earlier in methods) were fixed with 3% (vol/vol) paraformaldehyde (Electron Microscopy Sciences, 15714) and 0.1% (vol/vol ...
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bioRxiv - Cell Biology 2024Quote: ... Embedded cell pellets were washed five times for 3 min each in cacodylate buffer (0.15 M cacodylate with 4 mM CaCl2; Electron Microscopy Sciences, Cat. # 12300) before being post-fixed with osmium-ferricyanide (2 % OsO4 ...
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bioRxiv - Cell Biology 2022Quote: ... 3) three double-sided 9 x 0.12 mm round well electron microscopy grade double sided adhesive spacers (Electron Microscopy Sciences, Cat# 70327-8S), 4 ...
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bioRxiv - Evolutionary Biology 2024Quote: Negative staining grids were prepared using a standard protocol,86 pipetting 3-8 µL samples on glow discharged ultrathin carbon film supported copper grids (EMS CF400-Cu-UL), followed by staining with 2% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... using a Leica ARTOS 3D ultramicrotome, 2-3 74 nm thick sections were collected (4 levels, ∼ 8-10 μm apart) on copper grids (EMS, cat# G150-Cu) and imaging on TEM - 120kv JEOL 1400-Flash equipped with a 4k Gatan OneView digital camera ...
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bioRxiv - Physiology 2024Quote: ... Hearts were perfused for 3 min with PBS at 37°C to wash out the blood followed by a fixative solution of Glutaraldehyde 3% in 0.1M Cacodylate buffer pH7.4 (Electron Microscopy Sciences-cat#16538-15) for less than 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... The vibratome sections were stained with 1% tannic acid and 1% uranyl acetate (21700 and 22400, Electron Microscopy Sciences, Hatfield PA USA), dehydrated with ethanol ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with freshly prepared and filtered 1% tannic acid (TA, CAS#1401-55-4, EMS) in water using a Pelco BioWave microwave for seven 1-minute cycles alternating between 150 W and 0 W power ...
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bioRxiv - Neuroscience 2022Quote: ... and a 5-mm-diameter #1 glass coverslip (Electron Microscopy Sciences) was placed over the window and sealed to a custom-made metal ring using cyanoacrylate and dental cement ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was diluted to ~0.2 μM concentration in SEC buffer and 3 μL applied to negative glow discharged carbon coated copper grids (EMS, Cat No. CF300-Cu-UL). Sample was incubated on grids for 30 s ...
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bioRxiv - Genetics 2022Quote: ... Dissected gonads were fixed in 2 mL 3% paraformaldehyde (PFA) (10 mL 16% PFA, EM Grade, Electron Microscopy Sciences, Hatfield, PA Catalog No 15710) phosphate-buffered solution for 10 minutes at room temperature and then post-fixed with 2 mL 100% methanol (Gold-label from Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... were injected intraperitoneally in n=3 control and n=3 KA Nestin-GFP animals 30 minutes before perfusion with a PFA 4 %-Glutaraldehyde 0.5 % (Cat#16536-05, Electron Microscopy Sciences, Hatfield, PA, USA) mixture ...
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bioRxiv - Cell Biology 2024Quote: ... coli was washed 3 times in PBS and then immediately used live in phagocytic assays or fixed in 4% paraformaldehyde (PFA; Electron Microscopy Sciences, Hatfield, PA) in PBS and stored for a few weeks.
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bioRxiv - Cell Biology 2021Quote: ... 4 mM NaHCO3, 1 mM NaH2PO4, 5 mM trehalose, 10 mM sucrose, 5 mM HEPES, pH 7.5, prepared by Electron Microscopy Sciences) and then bathed in whole organ ex vivo culture medium with 10 µg/ml isradipine (Selleck Chemical LLC ...
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bioRxiv - Genomics 2020Quote: Ten million cells from two MSC preparations at early (P2 and 3) and late (P7 and 9) passages were cross-linked with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA, USA) for 10 min. ...
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bioRxiv - Cell Biology 2021Quote: Immediately after cold exposure mice were anaesthetized with isoflurane (3%) and perfused with heparinized saline followed by 4% paraformaldefyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA). Fat pads were carefully dissected and postfixed overnight in 4% PFA at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... with red anti-Alfa FluoTag for 30 min before being washed 3 times with PBS and fixed with 2% methanol-free PFA (Electron Microscopy Sciences, cat#157-8). Coverslips were directly mounted using Prolong Diamond Antifade mounting medium (ThermoFischer ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl sample was applied to copper 400-mesh grids with an ultra-thin layer of carbon (Electron Microscopy Sciences, cat. No. CF400-Cu-UL), that had been glow-discharged in air for 30 seconds ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated in cacodylate buffer containing 1% low molecular weight tannic acid for 30 min (Electron Microscopy Sciences) between the osmication step and the uranyl acetate step ...
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bioRxiv - Neuroscience 2024Quote: ... Poly-l-lysine coated chambers and coverslips were prepared by washing glass microscope slides and glass coverslips with acid alcohol (1% HCl in 70% ethanol) and placing 1:10 0.1% poly-l-lysine (Electron Microscopy Sciences, catalog #19320, Hatfield, PA) in ddH20 on slide for 1 hour before aspirating off and leaving covered in fume hood overnight (Suvarna SK 2013 ...
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bioRxiv - Neuroscience 2023Quote: ... a cylinder of tissue 2 mm in diameter and 3 mm in height for each area was collected with a tissue punch (EMS-core sampling tool, 69039-20, EMS), and ejected into PCR tubes filled with 100 µL of RNAlater and kept at −80°C for long-term storage ...
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bioRxiv - Microbiology 2021Quote: ... after receiving 1 μl of 5× concentrated 10 nm Au fiducial solution (EMS) in the back-side and being blotted from the back ...
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bioRxiv - Neuroscience 2023Quote: ... for 5 min and negative staining was performed with 1% uranyl acetate (EMS) for 1 min.
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bioRxiv - Cell Biology 2024Quote: ... treated with 0.1% w/v tannic acid (EMS) for 20min ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1 % tannic acid (w/v) (Electron Microscopy Sciences, 21700), 3 mM calcium chloride in 0.1 M sodium cacodylate buffer pH 7.4 (Ted Pella Incorporated ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1% sirius red in saturated picric acid (26357-02; EMS) for 60 min ...
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Exploring the Role of Spatial Confinement in Immune Cell Recruitment and Regeneration of Skin WoundsbioRxiv - Bioengineering 2023Quote: ... The slides were placed in Biebrich Scarlet-Acid Fuchsin (EMS) for 15 minutes and rinsed in DI water for 5 minutes ...
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bioRxiv - Genomics 2022Quote: ... 5% fixation buffer [5% paraformaldehyde (EMS, cat. no. 50-980-493), 1.25x dPBS] was added to each well ...
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bioRxiv - Immunology 2024Quote: ... Cells were rinsed twice in FACS buffer then fixed in 1% PFA (Electron Microscopy Sciences, 15714-5) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... sections were stained with phosphotungstatic acid (Electron Microscopy Science, Fort Washington, USA) and uranyl acetate (Electron Microscopy Science ...
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bioRxiv - Plant Biology 2023Quote: Roughly 30,000 Arabidopsis bak1-5 bkk1-1 cerk1-2 (bbc) seeds were mutagenized using 0.2% ethyl methanesulfonate (EMS). Mutagenized M1 seeds were sown on soil and allowed to grow to set seeds ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then negatively stained for 1 min with 5 μL of 2% uranyl acetate (Electron Microscopy Sciences, 541-09-3 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with ddH2O three times 5 min and incubated in 1% thiocarbohydrazide (EMS, cat# 2231-57-4) diluted in ddH2O for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μL of sample was applied to a glow discharged QF-R2/1 Cu 200M grid (Electron Microscopy Sciences). Grids were frozen into -185 °C liquid ethane using a Leica EM GP2 automatic plunge freezer equilibrated to 80% humidity and 10 °C with a 10 second sample incubation time and a 2.5 second blot time on Whatman 1 paper ...
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bioRxiv - Cell Biology 2020Quote: ... 300,000 cells were seeded on acid-washed 25mm cover glass (Electron Microscopy Sciences). After 24 h of growth ...
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bioRxiv - Immunology 2021Quote: ... Joint and spine samples were decalcified in 96% formic acid (Electron Microscopy Sciences). Paraffin-embedded sections were stained with hematoxylin and eosin (H&E) ...
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bioRxiv - Biophysics 2021Quote: ... after which 5-μL were deposited in a glow-discharged Quantifoil grid (200-mesh copper R2/1, Electron Microscopy Sciences), followed by manual blotting and plunge freezing in a 50/50 ethane propane mix (Airgas ...
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bioRxiv - Biochemistry 2024Quote: ... AFM samples were prepared by adsorbing 10 μl of 5 times diluted sample onto mica (Muscovite mica, V 1 quality, EMS) for 45 minutes ...
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bioRxiv - Pathology 2022Quote: ... sections were incubated with 0.1% Sirius Red in Saturated Picric Acid (Electron Microscopy Sciences) with 10% w/v Fast Green FCF (Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... fine-tipped forceps (Dumont #5, EMS). Holding the coverslip vertically ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA) and 5 μL of the mixture was applied to carbon-coated 300 mesh Cu grids (Electron Microscopy Sciences) after glow discharging for 1 min at −15 mA in an EasiGlo glow discharger (Ted Pella) ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL of the EV sample was adsorbed for 1 min to a carbon-coated grid (# CF400-CU, Electron Microscopy Sciences) that had been made hydrophilic by a 20-sec exposure to a glow discharge (25mA) ...
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bioRxiv - Microbiology 2022Quote: ... LSUCC0664 was grown to 106 cells mL-1 and 5 µL of culture was loaded onto a glow discharged 300 mesh carbon filmed grid (EMS:CF300-cu). We removed excess liquid with filter paper after 2 minutes and stained with 2% uranyl acetate (TED Pella Cat ...
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bioRxiv - Immunology 2024Quote: ... Vesicles were negatively stained with 200 µl of 1.5% phosphotungstic acid (PTA; Electron Microscopy Sciences). Excess liquid was blotted with filter paper and grids were air dried for at least 10 minutes ...