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Citations for Electron Microscopy Sciences :
451 - 500 of 1193 citations for 1 3 Fluorophenyl 5 oxopyrrolidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Slides were subsequently washed three more times for 5 minutes by immersion in 0.1% Tween-20 in PBS and mounted in EMS Shield Mount with DABCO (Electron Microscopy Sciences). Imaging was performed using a Carl Zeiss LSM710 confocal microscope at the microscopy imaging center (MIC ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μg of EVs was placed onto glow discharged 200-mesh formvar/carbon copper grids (Electron Microscopy Sciences, Hatfield Township, PA). The EVs were washed two times with water and then fixed in 2.5% glutaraldehyde ...
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bioRxiv - Plant Biology 2024Quote: Aliquots (5 µL) of resuspended P40 pellets were spotted onto Formvar and carbon-coated copper electron microscopy grids (Electron Microscopy Sciences) that were glow-discharged at 15mA for 60 seconds prior to application of samples ...
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bioRxiv - Neuroscience 2024Quote: ... and the hole in the bone was covered with a round cover glass (d = 5 mm; Electron Microscopy Sciences, 72296–05). A helicopter-shaped head plate (model 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... main inflorescences (not side branches) were cut and dissected with a Dumont tweezer (Electron Microscopy Sciences, style 5, no. 72701-D) to remove buds older than stage 9 or 10 ...
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bioRxiv - Neuroscience 2024Quote: ... transcardially perfused with PBS for 5 min and then with 10 mL of 4% PFA solution (Electron Microscopy Sciences, Cat#15714) for immunohistochemistry or with 10 mL of 2.5% glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: Cells were first fixed 1.5h at room temperature with 2x fixation solution (5% glutaraldehyde and 4% paraformaldehyde (EMS, Delta-Microscopies, France) in 0.1 M Sorensen buffer ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed three times (650xg, 5 min, RT) with Perm Buffer before fixation in 1.6% paraformaldehyde (PFA) (Electron Microscopy Sciences) in PBS overnight with 125nM Iridium (Fluidigm ...
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bioRxiv - Bioengineering 2024Quote: ... A 5 μL drop of the solution was deposited onto a glow-discharged formvar/carbon-coated copper grid (Electron Microscopy Sciences). The grid was incubated for 2 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... TolAQR (abs280=5) or TonB-ExbBD (abs280=1.4) were applied to a Quantifoil R1.2/1.3 300 mesh grid (Electron Microscopy Sciences, Protochips, Inc.) that had been glow discharged for 45 s at 15 mA (Pelco easiGlow ...
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bioRxiv - Immunology 2024Quote: ... slides were washed twice for 5 min in wash buffer and fixed in a solution of 2% glutaraldehyde (Electron Microscopy Sciences) solution in low-barium PBS for 5 min ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µl of protein complex was deposited on a holey copper grid (QUANTIFOIL R1.2/1.3, 200 mesh, EMS cat# Q250-CR1.3) or a carbon film copper grid (QUANTIFOIL R 1.2/1.3 ...
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bioRxiv - Neuroscience 2024Quote: ... at 1 : 1 for 30 min and 2 : 1 for 30 min and twice with pure Durcupan (Electron Microscopy Sciences, Hatfield, PA, USA) for 30 min each ...
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bioRxiv - Neuroscience 2023Quote: ... with 0.5% uranyl acetate (Uranyl acetate solution 1%, Electron Microscopy Sciences, cat.#22400-1) and 3% lead citrate (Ultrostain 2 ...
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bioRxiv - Genomics 2023Quote: ... a minimum of 1 million ESCs were fixed with 1% Formaldehyde (Electron Microscopy Sciences) for 10 min on a shaker at room temperature and quenched with Glycine 0.125M for 5 min at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... Preinfiltration was performed in a 1:1 mixture of ethanol:Technovit 7100 (Electron Microscopy Sciences) for 4 h under light agitation ...
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bioRxiv - Cell Biology 2023Quote: ... cells were post-fixed for 1 h in 1% osmium tetroxide (Electron Microscopy Sciences) in the same buffer at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... cells were post-fixed for 1 hour in 1% osmium tetroxide (Electron Microscopy Sciences) in the same buffer at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... containing 1% OsO4 (EMS, USA) and 1.5% C6FeK4N6 (EMS ...
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bioRxiv - Biophysics 2020Quote: ... and 1 % Uranyl acetate (EMS). These sections were then dehydrated by washing them serially with increasing concentrations of ethanol (30-100 % ethanol) ...
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bioRxiv - Plant Biology 2020Quote: ... thaliana max2-1 mutant (EMS mutant in Col-0-background ...
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bioRxiv - Cell Biology 2020Quote: ... and 1% uranyl acetate (EMS) in water with water washes between contrasting steps ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% Glutaraldehyde (GA) (EMS) in 0.1 M phosphate buffer of pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were diluted 10-fold in liposome buffer and 5 μL was applied to glow-discharged 400 mesh copper grids coated with formvar/carbon film (Electron Microscopy Sciences). After 1 min ...
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bioRxiv - Bioengineering 2021Quote: ... ∼5 μL of each sEV-particle complex was left to adhere onto formvar carbon coated copper Grid 200 mesh (Electron Microscopy Sciences) for 5 mins followed by 5 mins of negative staining with 2% aqueous uranyl acetate ...
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bioRxiv - Neuroscience 2020Quote: ... Each coverslip was mounted on a stub with carbon tape and samples were sputter coated with iridium at 10μA to a thickness of approximately 5 nm (EMS model 150T S). The coverslips were then examined on a Hitachi S4300 SEM (Hitachi High Technologies America Inc. ...
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... resuspended in plating media and plated at a density of 4-5×105 cells/12-mm coverslip (Electron Microscopy Sciences, Hatfield, PA) coated with poly-Ornithine (0.1mg/ml ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for 10-15 minutes at 4 °C before fixation in 2% paraformaldehyde solution for 5 minutes (Electron Microscopy Sciences). In the case of intracellular staining for TCRζ ...
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bioRxiv - Microbiology 2022Quote: ... infected cells (including supernatant) were collected from the 6-wells plate, centrifuged (500× g, 5 min, RT) and fixed with 4% PFA (Electron Microscopy Sciences) for 30min at RT ...
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bioRxiv - Bioengineering 2022Quote: ... We applied 5 μL RBC-EV sample to a 200 mesh copper grid coated with formvar carbon film (FCF200-Cu, Electron Microscopy Sciences) for 30 seconds ...
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bioRxiv - Molecular Biology 2024Quote: ... a 5 µL sample was spotted onto a glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences CF300-Cu-50), incubated for two minutes then gently blotted onto filter paper to remove excess solution ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were then washed three times in PBS for 5 minutes each prior to cover slipping using Fluorogel mounting medium (Electron Microscopy Sciences).
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bioRxiv - Microbiology 2023Quote: ... and 150 mM NaCl buffer and 5 µL was applied to glow-discharged 400 mesh copper grids coated with formvar/carbon film (Electron Microscopy Sciences). Grids were blotted after 1 min ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were stained with 10% Uranyl acetate (Sisco Research Laboratory Pvt. Limited, 81405) for 5-min and Sato’s lead citrate (Electron Microscopy Sciences, 17800) stain for 1-min ...
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bioRxiv - Immunology 2024Quote: ... and 5% glycerol and applied to glow-discharged grid with continuous carbon film on 400 square mesh copper EM grids (Electron Microscopy Sciences). The grids were stained with 2% uranyl formate (UF) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then gently washed with phosphate buffer saline (PBS) at room temperature for 5 minutes and then fixed with 4% paraformaldehyde (PFA) (cat:15710-S, Electron Microscopy Science) for 20 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of diluted phage solution was applied onto carbon-coated copper grids (FCF-200-Cu, Electron Microscopy Sciences, Hatfield, PA, USA). Following a 3-minute incubation ...
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bioRxiv - Cell Biology 2024Quote: ... cells grown on 13-mm round poly-L-lysine-coated coverslips were fixed by adding to the culture medium an equal volume of 4% PFA (Electron Microcopy Sciences) and 5% glutaraldehyde (GTA) (Electron Microscopy Sciences) in 0.2 M cacodylate pH 7.4 for 15 min at room temperature ...
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bioRxiv - Genetics 2021Quote: We screened for lst-1 or sygl-1 enhancers using a modified ethyl methanesulfonate (EMS) protocol (Brenner 1974) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were fixed at 14-DIV for 1-h in 1% glutaraldehyde (EMS, Hatfield, USA) diluted in 0.1M cacodylate buffer ...
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bioRxiv - Molecular Biology 2020Quote: 1×105 of U2OS-EpiGo cells were cultured on the #1 coverslip (Electron Microscopy Sciences) and added 500 ng/ml doxycycline for induction of dCas9 or dCas9-KRAB expression ...
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bioRxiv - Immunology 2021Quote: ... tissues were incubated in a 1:1 mixture of EMBed 812 (Electron Microscopy Sciences 14120) and propylene oxide ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed for 1 hour at room temperature with 1% OsO4 (Electron Microscopy Science, 19100), dehydrated in the ethanol/acetone series and embedded in Epon/Araldite epoxy embedding media (Electron Microscopy Science ...
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bioRxiv - Cell Biology 2022Quote: ... (6) infiltrated with 1:1 mixture of EMBED 812 (Electron Microscopy Sciences, Hatfield, PA, USA) and propylene oxide ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were rinsed three times with PBST for 5 min on ice and then embedded and frozen in Tissue Freezing Medium (Electron Microscopy Sciences, PA). Samples were sectioned (8μm ...
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bioRxiv - Biochemistry 2020Quote: End-point samples from wt α-syn aggregation assays (5 μL) were loaded onto carbon-coated 300 mesh copper grids (Electron microscopy Sciences) that had been glow discharged for 40 seconds using an PELCO easiGLOW™ glow discharge unit (Ted Pella Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... were deeply anesthetized by isoflurane and transcardially perfused with ice cold 1x PBS (∼5-7 ml) followed by 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences: 15713) in 1x PBS (∼5-10 ml ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µg of the sample was placed onto a glow discharged 200-mesh formvar/carbon copper grid (Electron Microscopy Sciences, Hatfield Township, PA) for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
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bioRxiv - Biophysics 2024Quote: ... the cells were fixed for 10 minutes in PEM buffer (80 mM PIPES, 5 mM EGTA, 2 mM MgCl2) containing 4% formaldehyde (FA) (32% sol. in glass ampoules, Electron Microscopy Sciences, #50980494) with 4% sucrose (Sigma ...