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Citations for Electron Microscopy Sciences :
201 - 250 of 1193 citations for 1 3 Fluorophenyl 5 oxopyrrolidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... a 3 μL sample was spotted onto a freshly glow-discharged carbon film 300 mesh copper grid (Electron Microscopy Sciences), incubated for 2 min ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed three times in sterile PBS and fixed in 3% paraformaldehyde (PFA) (Electron Microscopy Sciences, Hatfield, PA, USA) for 15 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... washed 3 times with PBS (Eurobio #CS1PBS01-01) and fixed and permeabilized using a 4% paraformaldehyde (Electron Microscopy Sciences # 15714), 0.2% Triton X-100 (Bio-Rad #1610407 ...
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bioRxiv - Microbiology 2023Quote: ... washed 3 times with PBS (Eurobio #CS1PBS01-01) then fixed and permeabilized using a 4% paraformaldehyde (Electron Microscopy Sciences # 15714), 0.2% Triton X-100 (Bio-Rad #1610407 ...
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bioRxiv - Neuroscience 2023Quote: Human PSD I pellets prepared from gestational week (GW) 23 samples were fixed with 3% glutaraldehyde (Electron Microscopy Sciences, EMS) for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 ul aliquots of the mixture were applied to UltrAu foil R1.2/1.3 gold 300-mesh grids (Electron Microscopy Sciences). Grids were then blotted and vitrified in liquid ethane cooled by liquid nitrogen using Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... FFPE sections (3 um) were collected and underwent antigen retrieval in a citrate (pH 6.0, Electron Microscopy Sciences, 62706-10) or Tris-EDTA (pH 9.0 ...
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bioRxiv - Biophysics 2024Quote: ... the flame sealed end of the tubing was immediately dipped into a parlodion solution (∼3 cm2 parlodion, Electron Microscopy Sciences [Hatfield ...
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bioRxiv - Biophysics 2024Quote: ... were diluted to[9 mg/ml and aliquots (3[μl) were applied to GF-0.6/1.0 300 Au-flat (Electron Microscopy Sciences). The cryo-EM grids were prepared using Thermo Fisher Vitrobot Mark IV at 8[°C and 100% humidity (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde, 2% paraformaldehyde, and 0.1% tannic acid prepared in 0.1 M phosphate buffer, pH 7, 15720, Electron Microscopy Sciences, Inc., PA). We washed the cells once with 0.1 M pH 7.2 phosphate buffer ...
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bioRxiv - Microbiology 2022Quote: ... 5 μL of 1% uranyl acetate in water (Electron Microscopy Sciences 22400-1) was added to the grid ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then rinsed 3 times on ice with ice-cold PBS and immediately fixed in 4% paraformaldehyde (EMS, 15710-S) for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to glow discharged holey carbon grids which was then back blotted before adding 3 µL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Physiology 2021Quote: The mouse liver was cut into 2-3 mm3 cubes and immediately immersed in a fixation solution containing 2.5% glutaraldehyde (Electron Microscopy Science) in 0.1 M cacodylate buffer pH 7.4 for 1 hour at room temperature and then overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL protein solution at 0.01 mg/mL was added to a glow-discharged carbon-coated copper grid (Electron Microscopy Science). Grids were stained in 2% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of sample was applied to glow-discharged (30 s) Quantifoil copper grids (R2/2, 200 mesh, Electron Microscopy Sciences), blotted on both sides for 4 s ...
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bioRxiv - Microbiology 2023Quote: ... 3 μL of concentrated pseudovirus sample was applied to a carbon-coated grid (2/2-4C C-flats; Electron Microscopy Sciences), blotted with filter paper ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated in 2x SSC twice for 3 minutes each before being placed in 50% formamide (Electron Microscopy Sciences)/2x SSC at room temperature for approximately 18 hours ...
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bioRxiv - Microbiology 2023Quote: ... 3 µl of bacterial suspension were placed on a freshly glow-discharged carbon covered electron microscopic support grid (EMS, Hatfield, PA) for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... 0.02% sodium azide (1hr rinse, 3 rinses) and was mounted on glass slides in anti-fade media (Citifluor, Electron Microscopy Sciences) and coverslipped ...
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bioRxiv - Biochemistry 2024Quote: ... Both sets of grids were then back blotted before adding 3 μL of 10 nm Protein-A-coated gold (EMS, USA) as fiducial markers for 3D reconstruction of tilt series ...
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bioRxiv - Microbiology 2023Quote: ... freshly made samples using the purification pipeline above were negatively stained with 3% uranyl acetate (Electron Microscopy Sciences [EMS], Cat# 22400). For TEM of macrophages ...
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bioRxiv - Microbiology 2023Quote: ... freshly made samples using the purification pipeline above were negatively stained with 3% uranyl acetate (Electron Microscopy Sciences [EMS], Cat# 22400). For TEM of macrophages ...
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bioRxiv - Biochemistry 2023Quote: ... Purified proteins were diluted to 0.005-0.01 mg/mL and 3.5 µL was applied onto copper grids with a thin layer of continuous carbon (approximately 3-4 nm, Electron Microscopy Sciences) that had been plasma-cleaned (Gatan Solarus ...
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bioRxiv - Microbiology 2024Quote: ... 3 µL sample was applied to glow discharged Quantifoil® R1.2/1.3 Cu 300 mesh grid (Electron Microscopy Sciences, PA, USA). The samples were then plunged into liquid ethane using a Vitrobot™ Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Copper grids were post stained with a 1.2% UA solution and a 3% lead citrate solution (Electron Microscopy Sciences Cat#22410) for 4 min each ...
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bioRxiv - Cell Biology 2023Quote: ... Retinas were then incubated in half dram vials containing 1% tannic acid (EMS, Cat# 21700) + 0.5% saponin diluted in 0.1M HEPES (pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... and stained at room temperature for 1 hour with 1% (wt/vol) low-molecular weight tannic acid (Electron Microscopy Science) in 01 ...
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bioRxiv - Neuroscience 2021Quote: ... 0.2% picric acid (EMS, USA) in 0.1M PB ...
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bioRxiv - Microbiology 2023Quote: ... 0.06% picric acid (EMS #19552), 20 mM Lysine (EMS #L5626) ...
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bioRxiv - Cell Biology 2023Quote: ... containing 0.1% tanic acid (EMS),1% glutaraldehyde (EMS ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were dehydrated through a series of increasing concentration of ethanol (30%, 50%, 70%, 90%, 3 times 100% anhydrous) and embedded in EMBed 812 epoxy resin (EMS, Hatfield, PA). After resin polymerization ...
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bioRxiv - Genetics 2021Quote: ... 3 μl of the diluted 3C library was deposited onto a plasma-clean Quantifoil R2/2 holey carbon grid (Electron Microscopy Sciences), blotted with a filter paper for 2 seconds ...
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bioRxiv - Bioengineering 2020Quote: ... were prepared by applying 3 µL of 10 mg/mL sample on pretreated lacey carbon 400 mesh TEM grids (Electron Microscopy Sciences). Following a 3 s blot ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Cell Biology 2022Quote: ... falciparum 3D7 culture was pelleted by centrifugation (1500g, 3 min, RT) and resuspended in freshly prepared PBS containing 4% EM-grade para-formaldehyde (PFA, Electron Microscopy Sciences) and 0.1% EM-grade GA (Sigma ...
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bioRxiv - Biochemistry 2020Quote: Freshly prepared filament samples (3 μL) were applied to glow-discharged Lacey carbon film grids (LC200-Cu; Electron Microscopy Sciences, Hatfield PA). The grid was blotted for 2.0 s using Whatman #1 filter paper (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µl of freshly purified full-length S protein was adsorbed to a glow-discharged carbon-coated copper grid (Electron Microscopy Sciences), washed with deionized water ...
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bioRxiv - Biophysics 2020Quote: ... the head was placed onto the scanner directly after cleaving the glued-on 3 mm Muscovite Mica disk (Electron Microscopy Sciences, Hatfield). The concentrated sample was diluted in imaging buffer to 60 µg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... PCW18 and PCW19 spinal cords were dissected out of the vertebral column and fixed for 2-3 hours at 4°C with 4% paraformaldehyde (PFA, Electron Microscopy Sciences), washed with PBS and transferred to a 30% sucrose solution for 48-72 hours ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was rinsed five times for 3 min in DDH2O and then dehydrated through graded alcohols into acetone and flat-embedded in Durcopan resin (Electron Microscopy Sciences) between mylar strips and placed in a 60°C oven for 48h.
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed using Karnovsky Fixative Reagent Grade (3% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer, pH 7.4) (Electron Microscopy Sciences, 15732-10) for 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... and cryo-ET grids were prepared by applying 3 μL of sample to glow-discharged (30 s) Lacey carbon grids (Electron Microscopy Sciences). Grids were blotted on both sides for 6 s with 0 blotting force and vitrified immediately by plunge freezing into liquid nitrogen-cooled liquid ethane with an FEI Mark IV Vitrobot cryo-sample plunger ...
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bioRxiv - Genomics 2023Quote: ... the cells were washed five times with PBS-T and post-fixed with 3% paraformaldehyde and 0.1% glutaraldehyde (Electron Microscopy Sciences #16120) in 1X PBS for 30 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... A 3-µl drop of the mixture was applied to a plasma-cleaned C-flat 2/2 holey carbon grid (Electron Microscopy Sciences), manually blotted and vitrified in liquid ethane with a homemade plunger.
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bioRxiv - Neuroscience 2022Quote: ... was perfused with 10 mL of 1x PBS followed by 30 mL of fixative 2% PFA/0.075% GA in PBS (32% PFA Alfa Aesar, 3% GA Electron Microscopy Science). Group 2 (indirect PFA ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were gradually dehydrated in an ethanol series (50%,75%,95%, 3×100%) and then embedded in EMbed-812 epoxy resin (Electron Microscopy Sciences), followed by polymerization for 48h at 60°C.Thin sections (70nm ...
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bioRxiv - Molecular Biology 2023Quote: Primed and naïve hESCs were prepared as described in the above section and fixed for 10 min in a 3% paraformaldehyde solution (Electron Microscopy Science). Cells were then permeabilized for 7 min with ice-cold PBS supplemented with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: Negatively stained samples were made by standard methods: 3 µl of sample was placed on a glow-discharged grid with ultrathin carbon layered on lacey carbon (Electron Microscopy Sciences), washed with three drops of water and stained with 1% uranyl acetate ...